This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.70 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 100,445 (3.9%) Reads that were too short: 52,064 (2.0%) Reads written (passing filters): 48,381 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,166,748 bp (1.4%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 100445 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1088 628.5 0 1088 7 11097 628.5 0 11097 8 1039 628.5 0 1039 9 12271 628.5 0 12271 10 234 628.5 0 234 11 36 628.5 0 36 12 33 628.5 0 33 13 33 628.5 0 33 14 47 628.5 0 47 15 33 628.5 0 33 16 92 628.5 0 92 17 73 628.5 0 73 18 228 628.5 0 228 19 33 628.5 0 33 20 38 628.5 0 38 21 46 628.5 0 46 22 35 628.5 0 35 23 24 628.5 0 24 24 28 628.5 0 28 25 74 628.5 0 74 26 36 628.5 0 36 27 39 628.5 0 39 28 38 628.5 0 38 29 32 628.5 0 32 30 40 628.5 0 40 31 38 628.5 0 38 32 37 628.5 0 37 33 30 628.5 0 30 34 38 628.5 0 38 35 43 628.5 0 43 36 28 628.5 0 28 37 39 628.5 0 39 38 29 628.5 0 29 39 23 628.5 0 23 40 25 628.5 0 25 41 33 628.5 0 33 42 33 628.5 0 33 43 32 628.5 0 32 44 30 628.5 0 30 45 34 628.5 0 34 46 27 628.5 0 27 47 26 628.5 0 26 48 52 628.5 0 52 49 42 628.5 0 42 50 121 628.5 0 121 51 72 628.5 0 72 52 36 628.5 0 36 53 46 628.5 0 46 54 46 628.5 0 46 55 27 628.5 0 27 56 41 628.5 0 41 57 32 628.5 0 32 58 28 628.5 0 28 59 37 628.5 0 37 60 43 628.5 0 43 61 63 628.5 0 63 62 42 628.5 0 42 63 53 628.5 0 53 64 35 628.5 0 35 65 39 628.5 0 39 66 56 628.5 0 56 67 446 628.5 0 446 68 479 628.5 0 479 69 377 628.5 0 377 70 26 628.5 0 26 71 65 628.5 0 65 72 55 628.5 0 55 73 45 628.5 0 45 74 54 628.5 0 54 75 36 628.5 0 36 76 38 628.5 0 38 77 40 628.5 0 40 78 33 628.5 0 33 79 28 628.5 0 28 80 36 628.5 0 36 81 40 628.5 0 40 82 37 628.5 0 37 83 44 628.5 0 44 84 41 628.5 0 41 85 87 628.5 0 87 86 178 628.5 0 178 87 68 628.5 0 68 88 49 628.5 0 49 89 47 628.5 0 47 90 63 628.5 0 63 91 27 628.5 0 27 92 47 628.5 0 47 93 30 628.5 0 30 94 52 628.5 0 52 95 40 628.5 0 40 96 56 628.5 0 56 97 59 628.5 0 59 98 58 628.5 0 58 99 34 628.5 0 34 100 38 628.5 0 38 101 27 628.5 0 27 102 24 628.5 0 24 103 37 628.5 0 37 104 31 628.5 0 31 105 24 628.5 0 24 106 48 628.5 0 48 107 32 628.5 0 32 108 28 628.5 0 28 109 30 628.5 0 30 110 45 628.5 0 45 111 38 628.5 0 38 112 33 628.5 0 33 113 56 628.5 0 56 114 35 628.5 0 35 115 36 628.5 0 36 116 46 628.5 0 46 117 60 628.5 0 60 118 110 628.5 0 110 119 109 628.5 0 109 120 118 628.5 0 118 121 113 628.5 0 113 122 94 628.5 0 94 123 70 628.5 0 70 124 39 628.5 0 39 125 37 628.5 0 37 126 78 628.5 0 78 127 87 628.5 0 87 128 143 628.5 0 143 129 105 628.5 0 105 130 62 628.5 0 62 131 141 628.5 0 141 132 110 628.5 0 110 133 126 628.5 0 126 134 100 628.5 0 100 135 52 628.5 0 52 136 51 628.5 0 51 137 32 628.5 0 32 138 51 628.5 0 51 139 50 628.5 0 50 140 48 628.5 0 48 141 34 628.5 0 34 142 43 628.5 0 43 143 35 628.5 0 35 144 49 628.5 0 49 145 72 628.5 0 72 146 84 628.5 0 84 147 108 628.5 0 108 148 91 628.5 0 91 149 81 628.5 0 81 150 81 628.5 0 81 151 103 628.5 0 103 152 516 628.5 0 516 153 574 628.5 0 574 154 798 628.5 0 798 155 999 628.5 0 999 156 52 628.5 0 52 157 54 628.5 0 54 158 144 628.5 0 144 159 392 628.5 0 392 160 191 628.5 0 191 161 87 628.5 0 87 162 81 628.5 0 81 163 356 628.5 0 356 164 561 628.5 0 561 165 716 628.5 0 716 166 287 628.5 0 287 167 141 628.5 0 141 168 64 628.5 0 64 169 73 628.5 0 73 170 44 628.5 0 44 171 52 628.5 0 52 172 73 628.5 0 73 173 87 628.5 0 87 174 119 628.5 0 119 175 126 628.5 0 126 176 66 628.5 0 66 177 116 628.5 0 116 178 141 628.5 0 141 179 191 628.5 0 191 180 265 628.5 0 265 181 152 628.5 0 152 182 130 628.5 0 130 183 616 628.5 0 616 184 879 628.5 0 879 185 651 628.5 0 651 186 492 628.5 0 492 187 197 628.5 0 197 188 61 628.5 0 61 189 84 628.5 0 84 190 82 628.5 0 82 191 121 628.5 0 121 192 412 628.5 0 412 193 595 628.5 0 595 194 446 628.5 0 446 195 367 628.5 0 367 196 65 628.5 0 65 197 73 628.5 0 73 198 221 628.5 0 221 199 332 628.5 0 332 200 349 628.5 0 349 201 277 628.5 0 277 202 194 628.5 0 194 203 125 628.5 0 125 204 103 628.5 0 103 205 170 628.5 0 170 206 218 628.5 0 218 207 230 628.5 0 230 208 181 628.5 0 181 209 174 628.5 0 174 210 113 628.5 0 113 211 85 628.5 0 85 212 145 628.5 0 145 213 116 628.5 0 116 214 210 628.5 0 210 215 265 628.5 0 265 216 285 628.5 0 285 217 582 628.5 0 582 218 923 628.5 0 923 219 9884 628.5 0 9884 220 6622 628.5 0 6622 221 15212 628.5 0 15212 222 366 628.5 0 366 223 112 628.5 0 112 224 114 628.5 0 114 225 47 628.5 0 47 226 69 628.5 0 69 227 48 628.5 0 48 228 84 628.5 0 84 229 89 628.5 0 89 230 118 628.5 0 118 231 114 628.5 0 114 232 96 628.5 0 96 233 85 628.5 0 85 234 134 628.5 0 134 235 179 628.5 0 179 236 120 628.5 0 120 237 175 628.5 0 175 238 143 628.5 0 143 239 193 628.5 0 193 240 231 628.5 0 231 241 168 628.5 0 168 242 95 628.5 0 95 243 61 628.5 0 61 244 82 628.5 0 82 245 64 628.5 0 64 246 83 628.5 0 83 247 112 628.5 0 112 248 344 628.5 0 344 249 621 628.5 0 621 250 588 628.5 0 588 251 277 628.5 0 277 252 213 628.5 0 213 253 265 628.5 0 265 254 352 628.5 0 352 255 141 628.5 0 141 256 103 628.5 0 103 257 98 628.5 0 98 258 110 628.5 0 110 259 122 628.5 0 122 260 107 628.5 0 107 261 93 628.5 0 93 262 99 628.5 0 99 263 90 628.5 0 90 264 98 628.5 0 98 265 77 628.5 0 77 266 101 628.5 0 101 267 158 628.5 0 158 268 154 628.5 0 154 269 150 628.5 0 150 270 117 628.5 0 117 271 93 628.5 0 93 272 215 628.5 0 215 273 325 628.5 0 325 274 251 628.5 0 251 275 179 628.5 0 179 276 88 628.5 0 88 277 137 628.5 0 137 278 259 628.5 0 259 279 859 628.5 0 859 280 1061 628.5 0 1061 281 1039 628.5 0 1039 282 310 628.5 0 310 283 146 628.5 0 146 284 206 628.5 0 206 285 134 628.5 0 134 286 132 628.5 0 132 287 173 628.5 0 173 288 191 628.5 0 191 289 300 628.5 0 300 290 131 628.5 0 131 291 116 628.5 0 116 292 257 628.5 0 257 293 250 628.5 0 250 294 308 628.5 0 308 295 170 628.5 0 170 296 157 628.5 0 157 297 146 628.5 0 146 298 150 628.5 0 150 299 215 628.5 0 215 300 280 628.5 0 280 301 289 628.5 0 289 Finished in 2.11 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 48,381 Reads with adapters: 13,528 (28.0%) Reads that were too short: 188 (0.4%) Reads written (passing filters): 13,340 (27.6%) Total basepairs processed: 11,166,748 bp Total written (filtered): 3,642,135 bp (32.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13528 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 20 0.0 3 0 0 13 7 16 143 0.0 3 0 120 12 11 17 12842 0.0 3 11640 930 228 44 18 43 0.0 3 1 28 7 7 19 53 0.0 3 3 2 21 27 20 53 0.0 3 2 3 0 48 21 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 0 2 26 5 0.0 3 0 0 0 5 27 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 34 4 0.0 3 0 0 0 4 35 2 0.0 3 0 0 0 2 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 39 15 0.0 3 0 0 0 15 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 5 0.0 3 0 0 0 5 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 1 48 2 0.0 3 0 0 0 2 50 6 0.0 3 0 0 0 6 52 5 0.0 3 0 0 0 5 53 3 0.0 3 0 0 0 3 54 11 0.0 3 0 0 0 11 55 4 0.0 3 0 0 1 3 56 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 59 8 0.0 3 0 0 1 7 61 4 0.0 3 0 0 0 4 62 2 0.0 3 0 0 0 2 63 6 0.0 3 0 0 0 6 66 1 0.0 3 0 0 0 1 68 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 1 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 3 0.0 3 0 0 0 3 85 3 0.0 3 0 0 1 2 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 90 11 0.0 3 0 0 0 11 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 98 5 0.0 3 0 0 0 5 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 1 0 1 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 11 0.0 3 0 0 0 11 105 3 0.0 3 0 0 1 2 106 5 0.0 3 0 0 0 5 107 4 0.0 3 0 0 0 4 108 2 0.0 3 0 0 0 2 109 52 0.0 3 0 0 0 52 110 3 0.0 3 0 0 1 2 111 3 0.0 3 0 0 0 3 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 116 30 0.0 3 0 0 0 30 118 1 0.0 3 0 0 0 1 119 6 0.0 3 0 0 0 6 120 12 0.0 3 0 0 1 11 121 1 0.0 3 0 0 0 1 122 9 0.0 3 0 0 0 9 123 5 0.0 3 0 0 0 5 124 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 7 0.0 3 0 0 0 7 141 4 0.0 3 0 0 0 4 143 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 1 1 165 2 0.0 3 0 0 1 1 169 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 1 178 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.46 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 13,340 Reads with adapters: 101 (0.8%) Reads written (passing filters): 13,340 (100.0%) Total basepairs processed: 3,642,135 bp Total written (filtered): 3,641,801 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 101 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.9% C: 5.9% G: 85.1% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 71 208.4 0 71 4 29 52.1 0 29 5 1 13.0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.