This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.86 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 441,032 (17.1%) Reads that were too short: 31,211 (1.2%) Reads written (passing filters): 409,821 (15.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 91,490,916 bp (11.8%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 441032 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 484 628.5 0 484 7 1749 628.5 0 1749 8 27060 628.5 0 27060 9 25046 628.5 0 25046 10 28059 628.5 0 28059 11 61 628.5 0 61 12 109 628.5 0 109 13 36 628.5 0 36 14 15 628.5 0 15 15 29 628.5 0 29 16 50 628.5 0 50 17 51 628.5 0 51 18 34 628.5 0 34 19 39 628.5 0 39 20 39 628.5 0 39 21 45 628.5 0 45 22 58 628.5 0 58 23 70 628.5 0 70 24 76 628.5 0 76 25 56 628.5 0 56 26 74 628.5 0 74 27 81 628.5 0 81 28 42 628.5 0 42 29 51 628.5 0 51 30 54 628.5 0 54 31 73 628.5 0 73 32 219 628.5 0 219 33 37 628.5 0 37 34 26 628.5 0 26 35 23 628.5 0 23 36 24 628.5 0 24 37 37 628.5 0 37 38 121 628.5 0 121 39 121 628.5 0 121 40 249 628.5 0 249 41 379 628.5 0 379 42 67 628.5 0 67 43 50 628.5 0 50 44 24 628.5 0 24 45 39 628.5 0 39 46 24 628.5 0 24 47 44 628.5 0 44 48 114 628.5 0 114 49 99 628.5 0 99 50 76 628.5 0 76 51 110 628.5 0 110 52 163 628.5 0 163 53 89 628.5 0 89 54 96 628.5 0 96 55 28 628.5 0 28 56 32 628.5 0 32 57 77 628.5 0 77 58 43 628.5 0 43 59 47 628.5 0 47 60 65 628.5 0 65 61 39 628.5 0 39 62 63 628.5 0 63 63 49 628.5 0 49 64 30 628.5 0 30 65 47 628.5 0 47 66 121 628.5 0 121 67 71 628.5 0 71 68 172 628.5 0 172 69 87 628.5 0 87 70 69 628.5 0 69 71 52 628.5 0 52 72 179 628.5 0 179 73 83 628.5 0 83 74 126 628.5 0 126 75 161 628.5 0 161 76 228 628.5 0 228 77 360 628.5 0 360 78 740 628.5 0 740 79 1417 628.5 0 1417 80 439 628.5 0 439 81 575 628.5 0 575 82 917 628.5 0 917 83 462 628.5 0 462 84 465 628.5 0 465 85 970 628.5 0 970 86 1251 628.5 0 1251 87 1342 628.5 0 1342 88 1277 628.5 0 1277 89 2093 628.5 0 2093 90 2964 628.5 0 2964 91 4070 628.5 0 4070 92 89779 628.5 0 89779 93 107812 628.5 0 107812 94 91298 628.5 0 91298 95 1759 628.5 0 1759 96 47 628.5 0 47 97 87 628.5 0 87 98 176 628.5 0 176 99 60 628.5 0 60 100 26 628.5 0 26 101 14 628.5 0 14 102 24 628.5 0 24 103 26 628.5 0 26 104 20 628.5 0 20 105 20 628.5 0 20 106 18 628.5 0 18 107 30 628.5 0 30 108 34 628.5 0 34 109 39 628.5 0 39 110 85 628.5 0 85 111 181 628.5 0 181 112 125 628.5 0 125 113 32 628.5 0 32 114 64 628.5 0 64 115 73 628.5 0 73 116 63 628.5 0 63 117 89 628.5 0 89 118 45 628.5 0 45 119 294 628.5 0 294 120 16 628.5 0 16 121 19 628.5 0 19 122 7 628.5 0 7 123 12 628.5 0 12 124 16 628.5 0 16 125 34 628.5 0 34 126 20 628.5 0 20 127 17 628.5 0 17 128 36 628.5 0 36 129 32 628.5 0 32 130 55 628.5 0 55 131 62 628.5 0 62 132 23 628.5 0 23 133 35 628.5 0 35 134 20 628.5 0 20 135 34 628.5 0 34 136 46 628.5 0 46 137 35 628.5 0 35 138 59 628.5 0 59 139 33 628.5 0 33 140 19 628.5 0 19 141 85 628.5 0 85 142 30 628.5 0 30 143 43 628.5 0 43 144 57 628.5 0 57 145 82 628.5 0 82 146 33 628.5 0 33 147 30 628.5 0 30 148 43 628.5 0 43 149 24 628.5 0 24 150 31 628.5 0 31 151 18 628.5 0 18 152 23 628.5 0 23 153 23 628.5 0 23 154 19 628.5 0 19 155 21 628.5 0 21 156 24 628.5 0 24 157 35 628.5 0 35 158 41 628.5 0 41 159 26 628.5 0 26 160 29 628.5 0 29 161 20 628.5 0 20 162 23 628.5 0 23 163 25 628.5 0 25 164 18 628.5 0 18 165 16 628.5 0 16 166 17 628.5 0 17 167 17 628.5 0 17 168 18 628.5 0 18 169 20 628.5 0 20 170 14 628.5 0 14 171 28 628.5 0 28 172 25 628.5 0 25 173 27 628.5 0 27 174 12 628.5 0 12 175 27 628.5 0 27 176 41 628.5 0 41 177 61 628.5 0 61 178 260 628.5 0 260 179 212 628.5 0 212 180 154 628.5 0 154 181 38 628.5 0 38 182 31 628.5 0 31 183 40 628.5 0 40 184 39 628.5 0 39 185 47 628.5 0 47 186 74 628.5 0 74 187 148 628.5 0 148 188 226 628.5 0 226 189 97 628.5 0 97 190 177 628.5 0 177 191 180 628.5 0 180 192 116 628.5 0 116 193 71 628.5 0 71 194 116 628.5 0 116 195 113 628.5 0 113 196 143 628.5 0 143 197 125 628.5 0 125 198 182 628.5 0 182 199 247 628.5 0 247 200 377 628.5 0 377 201 5549 628.5 0 5549 202 5452 628.5 0 5452 203 6962 628.5 0 6962 204 236 628.5 0 236 205 303 628.5 0 303 206 748 628.5 0 748 207 615 628.5 0 615 208 513 628.5 0 513 209 137 628.5 0 137 210 87 628.5 0 87 211 78 628.5 0 78 212 71 628.5 0 71 213 68 628.5 0 68 214 59 628.5 0 59 215 107 628.5 0 107 216 170 628.5 0 170 217 150 628.5 0 150 218 116 628.5 0 116 219 85 628.5 0 85 220 78 628.5 0 78 221 186 628.5 0 186 222 303 628.5 0 303 223 220 628.5 0 220 224 137 628.5 0 137 225 394 628.5 0 394 226 915 628.5 0 915 227 1077 628.5 0 1077 228 754 628.5 0 754 229 670 628.5 0 670 230 47 628.5 0 47 231 47 628.5 0 47 232 43 628.5 0 43 233 36 628.5 0 36 234 23 628.5 0 23 235 23 628.5 0 23 236 77 628.5 0 77 237 73 628.5 0 73 238 73 628.5 0 73 239 26 628.5 0 26 240 21 628.5 0 21 241 19 628.5 0 19 242 15 628.5 0 15 243 42 628.5 0 42 244 28 628.5 0 28 245 44 628.5 0 44 246 69 628.5 0 69 247 57 628.5 0 57 248 42 628.5 0 42 249 29 628.5 0 29 250 38 628.5 0 38 251 35 628.5 0 35 252 31 628.5 0 31 253 67 628.5 0 67 254 33 628.5 0 33 255 47 628.5 0 47 256 46 628.5 0 46 257 45 628.5 0 45 258 69 628.5 0 69 259 362 628.5 0 362 260 553 628.5 0 553 261 582 628.5 0 582 262 373 628.5 0 373 263 169 628.5 0 169 264 67 628.5 0 67 265 95 628.5 0 95 266 35 628.5 0 35 267 27 628.5 0 27 268 31 628.5 0 31 269 61 628.5 0 61 270 123 628.5 0 123 271 240 628.5 0 240 272 507 628.5 0 507 273 112 628.5 0 112 274 49 628.5 0 49 275 52 628.5 0 52 276 84 628.5 0 84 277 87 628.5 0 87 278 85 628.5 0 85 279 50 628.5 0 50 280 128 628.5 0 128 281 108 628.5 0 108 282 129 628.5 0 129 283 74 628.5 0 74 284 161 628.5 0 161 285 123 628.5 0 123 286 182 628.5 0 182 287 107 628.5 0 107 288 50 628.5 0 50 289 36 628.5 0 36 290 52 628.5 0 52 291 36 628.5 0 36 292 51 628.5 0 51 293 68 628.5 0 68 294 64 628.5 0 64 295 122 628.5 0 122 296 149 628.5 0 149 297 783 628.5 0 783 298 851 628.5 0 851 299 2189 628.5 0 2189 300 49 628.5 0 49 301 19 628.5 0 19 Finished in 15.36 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 409,821 Reads with adapters: 33,094 (8.1%) Reads that were too short: 896 (0.2%) Reads written (passing filters): 32,198 (7.9%) Total basepairs processed: 91,490,916 bp Total written (filtered): 7,810,653 bp (8.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33094 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 30 0.0 3 0 0 17 13 16 280 0.0 3 0 198 55 27 17 25055 0.0 3 22529 1983 440 103 18 77 0.0 3 6 58 6 7 19 148 0.0 3 16 2 73 57 20 81 0.0 3 5 1 1 74 23 1 0.0 3 0 0 0 1 25 2 0.0 3 0 0 0 2 26 4 0.0 3 0 0 0 4 27 1 0.0 3 0 1 29 2 0.0 3 0 0 0 2 30 19 0.0 3 0 0 0 19 31 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 42 5 0.0 3 0 0 0 5 44 2 0.0 3 0 0 0 2 45 5 0.0 3 0 0 0 5 46 1 0.0 3 0 0 1 47 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 6 0.0 3 0 0 0 6 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 2 0.0 3 0 0 0 2 71 2 0.0 3 0 0 0 2 72 1 0.0 3 0 0 0 1 74 54 0.0 3 0 0 0 54 75 35 0.0 3 0 0 0 35 76 13 0.0 3 0 0 0 13 77 36 0.0 3 0 0 2 34 78 4 0.0 3 0 0 0 4 79 220 0.0 3 0 0 4 216 80 11 0.0 3 0 0 0 11 81 5 0.0 3 0 0 0 5 82 32 0.0 3 0 0 0 32 83 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 95 3 0.0 3 0 0 0 3 96 4 0.0 3 0 0 1 3 97 2 0.0 3 0 0 0 2 98 23 0.0 3 0 0 0 23 99 4048 0.0 3 0 0 38 4010 100 1315 0.0 3 0 0 9 1306 101 5 0.0 3 0 0 1 4 102 368 0.0 3 0 0 17 351 103 201 0.0 3 0 0 5 196 104 4 0.0 3 0 0 0 4 105 14 0.0 3 0 0 0 14 107 2 0.0 3 0 0 0 2 108 1 0.0 3 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 7 0.0 3 0 0 0 7 123 15 0.0 3 0 0 0 15 124 16 0.0 3 0 0 0 16 125 47 0.0 3 0 0 0 47 126 14 0.0 3 0 0 0 14 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 6 0.0 3 0 0 0 6 131 2 0.0 3 0 0 0 2 132 6 0.0 3 0 0 0 6 134 4 0.0 3 0 0 0 4 135 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 144 5 0.0 3 1 2 1 1 145 3 0.0 3 0 0 0 3 146 5 0.0 3 0 0 0 5 147 5 0.0 3 0 0 0 5 149 6 0.0 3 0 0 0 6 150 71 0.0 3 0 0 1 70 151 28 0.0 3 0 0 1 27 152 7 0.0 3 0 0 0 7 153 18 0.0 3 0 0 2 16 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 6 0.0 3 0 0 0 6 158 12 0.0 3 0 0 1 11 159 22 0.0 3 0 0 0 22 160 23 0.0 3 0 0 0 23 161 4 0.0 3 0 0 1 3 162 3 0.0 3 0 0 0 3 163 5 0.0 3 0 0 0 5 167 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 174 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 176 4 0.0 3 0 0 0 4 177 5 0.0 3 0 0 0 5 178 4 0.0 3 0 0 1 3 179 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 0 2 183 4 0.0 3 0 0 0 4 184 2 0.0 3 0 0 0 2 185 4 0.0 3 0 0 1 3 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 190 8 0.0 3 0 0 0 8 191 39 0.0 3 0 0 3 36 192 8 0.0 3 0 0 0 8 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 4 0.0 3 0 0 0 4 197 1 0.0 3 0 0 0 1 199 32 0.0 3 0 0 0 32 200 242 0.0 3 0 0 7 235 201 85 0.0 3 0 0 4 81 202 25 0.0 3 0 0 1 24 203 2 0.0 3 0 0 0 2 204 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 4 0.0 3 0 0 0 4 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 8 0.0 3 0 0 0 8 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 214 7 0.0 3 0 0 0 7 215 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 247 2 0.0 3 0 0 0 2 253 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 267 3 0.0 3 0 0 0 3 268 2 0.0 3 0 0 0 2 271 1 0.0 3 0 0 0 1 276 4 0.0 3 0 0 0 4 277 2 0.0 3 0 0 0 2 278 4 0.0 3 0 0 1 3 279 4 0.0 3 0 0 0 4 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.04 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 32,198 Reads with adapters: 135 (0.4%) Reads written (passing filters): 32,198 (100.0%) Total basepairs processed: 7,810,653 bp Total written (filtered): 7,810,212 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.2% C: 17.0% G: 33.3% T: 4.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 116 503.1 0 116 4 14 125.8 0 14 5 3 31.4 0 3 11 2 0.0 1 0 2