This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.75 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 415,071 (16.1%) Reads that were too short: 29,186 (1.1%) Reads written (passing filters): 385,885 (15.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 86,983,949 bp (11.2%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 415071 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 377 628.5 0 377 7 1696 628.5 0 1696 8 26034 628.5 0 26034 9 30513 628.5 0 30513 10 31261 628.5 0 31261 11 48 628.5 0 48 12 45 628.5 0 45 13 32 628.5 0 32 14 18 628.5 0 18 15 31 628.5 0 31 16 30 628.5 0 30 17 43 628.5 0 43 18 27 628.5 0 27 19 50 628.5 0 50 20 30 628.5 0 30 21 43 628.5 0 43 22 48 628.5 0 48 23 73 628.5 0 73 24 98 628.5 0 98 25 62 628.5 0 62 26 65 628.5 0 65 27 96 628.5 0 96 28 41 628.5 0 41 29 39 628.5 0 39 30 38 628.5 0 38 31 45 628.5 0 45 32 62 628.5 0 62 33 26 628.5 0 26 34 32 628.5 0 32 35 32 628.5 0 32 36 21 628.5 0 21 37 21 628.5 0 21 38 30 628.5 0 30 39 70 628.5 0 70 40 98 628.5 0 98 41 147 628.5 0 147 42 68 628.5 0 68 43 41 628.5 0 41 44 27 628.5 0 27 45 29 628.5 0 29 46 40 628.5 0 40 47 42 628.5 0 42 48 92 628.5 0 92 49 76 628.5 0 76 50 62 628.5 0 62 51 44 628.5 0 44 52 76 628.5 0 76 53 53 628.5 0 53 54 65 628.5 0 65 55 32 628.5 0 32 56 40 628.5 0 40 57 78 628.5 0 78 58 34 628.5 0 34 59 63 628.5 0 63 60 60 628.5 0 60 61 39 628.5 0 39 62 69 628.5 0 69 63 57 628.5 0 57 64 32 628.5 0 32 65 50 628.5 0 50 66 111 628.5 0 111 67 74 628.5 0 74 68 193 628.5 0 193 69 106 628.5 0 106 70 69 628.5 0 69 71 43 628.5 0 43 72 129 628.5 0 129 73 96 628.5 0 96 74 111 628.5 0 111 75 193 628.5 0 193 76 232 628.5 0 232 77 364 628.5 0 364 78 720 628.5 0 720 79 1426 628.5 0 1426 80 413 628.5 0 413 81 449 628.5 0 449 82 939 628.5 0 939 83 458 628.5 0 458 84 407 628.5 0 407 85 1046 628.5 0 1046 86 1269 628.5 0 1269 87 1238 628.5 0 1238 88 1019 628.5 0 1019 89 1612 628.5 0 1612 90 2213 628.5 0 2213 91 2716 628.5 0 2716 92 78134 628.5 0 78134 93 95134 628.5 0 95134 94 88257 628.5 0 88257 95 1729 628.5 0 1729 96 35 628.5 0 35 97 51 628.5 0 51 98 58 628.5 0 58 99 27 628.5 0 27 100 28 628.5 0 28 101 19 628.5 0 19 102 32 628.5 0 32 103 17 628.5 0 17 104 19 628.5 0 19 105 15 628.5 0 15 106 14 628.5 0 14 107 24 628.5 0 24 108 32 628.5 0 32 109 43 628.5 0 43 110 52 628.5 0 52 111 103 628.5 0 103 112 59 628.5 0 59 113 14 628.5 0 14 114 43 628.5 0 43 115 24 628.5 0 24 116 22 628.5 0 22 117 65 628.5 0 65 118 37 628.5 0 37 119 312 628.5 0 312 120 21 628.5 0 21 121 21 628.5 0 21 122 12 628.5 0 12 123 16 628.5 0 16 124 18 628.5 0 18 125 40 628.5 0 40 126 24 628.5 0 24 127 15 628.5 0 15 128 48 628.5 0 48 129 25 628.5 0 25 130 26 628.5 0 26 131 26 628.5 0 26 132 22 628.5 0 22 133 22 628.5 0 22 134 10 628.5 0 10 135 22 628.5 0 22 136 44 628.5 0 44 137 33 628.5 0 33 138 46 628.5 0 46 139 27 628.5 0 27 140 17 628.5 0 17 141 67 628.5 0 67 142 17 628.5 0 17 143 37 628.5 0 37 144 26 628.5 0 26 145 31 628.5 0 31 146 23 628.5 0 23 147 21 628.5 0 21 148 26 628.5 0 26 149 19 628.5 0 19 150 20 628.5 0 20 151 25 628.5 0 25 152 21 628.5 0 21 153 24 628.5 0 24 154 23 628.5 0 23 155 24 628.5 0 24 156 24 628.5 0 24 157 31 628.5 0 31 158 30 628.5 0 30 159 33 628.5 0 33 160 20 628.5 0 20 161 19 628.5 0 19 162 14 628.5 0 14 163 24 628.5 0 24 164 22 628.5 0 22 165 16 628.5 0 16 166 17 628.5 0 17 167 25 628.5 0 25 168 32 628.5 0 32 169 25 628.5 0 25 170 30 628.5 0 30 171 31 628.5 0 31 172 27 628.5 0 27 173 28 628.5 0 28 174 22 628.5 0 22 175 31 628.5 0 31 176 38 628.5 0 38 177 81 628.5 0 81 178 292 628.5 0 292 179 180 628.5 0 180 180 136 628.5 0 136 181 24 628.5 0 24 182 44 628.5 0 44 183 44 628.5 0 44 184 39 628.5 0 39 185 50 628.5 0 50 186 65 628.5 0 65 187 116 628.5 0 116 188 255 628.5 0 255 189 101 628.5 0 101 190 218 628.5 0 218 191 212 628.5 0 212 192 140 628.5 0 140 193 58 628.5 0 58 194 139 628.5 0 139 195 138 628.5 0 138 196 133 628.5 0 133 197 103 628.5 0 103 198 155 628.5 0 155 199 208 628.5 0 208 200 296 628.5 0 296 201 6444 628.5 0 6444 202 6116 628.5 0 6116 203 8649 628.5 0 8649 204 261 628.5 0 261 205 352 628.5 0 352 206 676 628.5 0 676 207 544 628.5 0 544 208 298 628.5 0 298 209 122 628.5 0 122 210 111 628.5 0 111 211 67 628.5 0 67 212 77 628.5 0 77 213 59 628.5 0 59 214 59 628.5 0 59 215 70 628.5 0 70 216 86 628.5 0 86 217 109 628.5 0 109 218 90 628.5 0 90 219 84 628.5 0 84 220 69 628.5 0 69 221 219 628.5 0 219 222 352 628.5 0 352 223 199 628.5 0 199 224 84 628.5 0 84 225 456 628.5 0 456 226 930 628.5 0 930 227 881 628.5 0 881 228 761 628.5 0 761 229 346 628.5 0 346 230 23 628.5 0 23 231 14 628.5 0 14 232 19 628.5 0 19 233 19 628.5 0 19 234 16 628.5 0 16 235 24 628.5 0 24 236 46 628.5 0 46 237 35 628.5 0 35 238 33 628.5 0 33 239 24 628.5 0 24 240 20 628.5 0 20 241 19 628.5 0 19 242 23 628.5 0 23 243 37 628.5 0 37 244 16 628.5 0 16 245 36 628.5 0 36 246 39 628.5 0 39 247 40 628.5 0 40 248 27 628.5 0 27 249 27 628.5 0 27 250 25 628.5 0 25 251 30 628.5 0 30 252 19 628.5 0 19 253 27 628.5 0 27 254 28 628.5 0 28 255 39 628.5 0 39 256 41 628.5 0 41 257 45 628.5 0 45 258 83 628.5 0 83 259 376 628.5 0 376 260 647 628.5 0 647 261 652 628.5 0 652 262 387 628.5 0 387 263 151 628.5 0 151 264 61 628.5 0 61 265 30 628.5 0 30 266 29 628.5 0 29 267 31 628.5 0 31 268 25 628.5 0 25 269 34 628.5 0 34 270 54 628.5 0 54 271 136 628.5 0 136 272 366 628.5 0 366 273 101 628.5 0 101 274 24 628.5 0 24 275 19 628.5 0 19 276 69 628.5 0 69 277 51 628.5 0 51 278 61 628.5 0 61 279 42 628.5 0 42 280 77 628.5 0 77 281 74 628.5 0 74 282 60 628.5 0 60 283 36 628.5 0 36 284 146 628.5 0 146 285 52 628.5 0 52 286 121 628.5 0 121 287 59 628.5 0 59 288 23 628.5 0 23 289 28 628.5 0 28 290 36 628.5 0 36 291 27 628.5 0 27 292 23 628.5 0 23 293 53 628.5 0 53 294 35 628.5 0 35 295 51 628.5 0 51 296 45 628.5 0 45 297 418 628.5 0 418 298 447 628.5 0 447 299 971 628.5 0 971 300 38 628.5 0 38 301 19 628.5 0 19 Finished in 16.51 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 385,885 Reads with adapters: 28,453 (7.4%) Reads that were too short: 73 (0.0%) Reads written (passing filters): 28,380 (7.4%) Total basepairs processed: 86,983,949 bp Total written (filtered): 7,670,362 bp (8.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 28453 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 18 0.0 3 0 0 18 19 514 0.0 3 0 508 5 1 20 27276 0.0 4 26868 392 15 1 21 56 0.0 4 7 48 1 22 13 0.0 4 7 1 5 23 18 0.0 4 1 0 0 17 24 1 0.0 4 1 25 1 0.0 4 1 27 2 0.0 4 2 34 1 0.0 4 1 51 1 0.0 4 1 68 9 0.0 4 0 0 0 0 9 89 1 0.0 4 1 92 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 1 108 1 0.0 4 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 1 125 3 0.0 4 0 0 0 0 3 126 5 0.0 4 0 0 0 0 5 127 456 0.0 4 0 0 0 0 456 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 1 131 1 0.0 4 0 0 0 0 1 136 7 0.0 4 0 0 0 0 7 137 1 0.0 4 0 0 0 0 1 139 2 0.0 4 0 0 0 0 2 149 3 0.0 4 0 0 0 0 3 150 7 0.0 4 0 0 0 0 7 151 35 0.0 4 0 0 0 0 35 174 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 1 186 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.02 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 28,380 Reads with adapters: 3,912 (13.8%) Reads written (passing filters): 28,380 (100.0%) Total basepairs processed: 7,670,362 bp Total written (filtered): 7,631,425 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3912 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 3.6% G: 93.5% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 443.4 0 16 4 7 110.9 0 7 6 1 6.9 0 1 8 2 0.4 0 2 9 1633 0.1 0 46 1587 10 1798 0.0 1 70 1728 11 438 0.0 1 7 431 12 7 0.0 1 0 7 13 2 0.0 1 0 2 122 1 0.0 1 1 150 5 0.0 1 0 5 154 1 0.0 1 0 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.