This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.99 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 262,844 (10.2%) Reads that were too short: 17,347 (0.7%) Reads written (passing filters): 245,497 (9.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 53,882,363 bp (7.0%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 262844 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 212 628.5 0 212 7 5869 628.5 0 5869 8 3043 628.5 0 3043 9 13 628.5 0 13 10 55 628.5 0 55 11 75 628.5 0 75 12 13 628.5 0 13 13 30 628.5 0 30 14 9 628.5 0 9 15 11 628.5 0 11 16 6 628.5 0 6 17 8 628.5 0 8 18 20 628.5 0 20 19 20 628.5 0 20 20 15 628.5 0 15 21 42 628.5 0 42 22 19 628.5 0 19 23 24 628.5 0 24 24 9 628.5 0 9 25 8 628.5 0 8 26 10 628.5 0 10 27 22 628.5 0 22 28 62 628.5 0 62 29 23 628.5 0 23 30 18 628.5 0 18 31 22 628.5 0 22 32 16 628.5 0 16 33 23 628.5 0 23 34 11 628.5 0 11 35 31 628.5 0 31 36 20 628.5 0 20 37 5 628.5 0 5 38 12 628.5 0 12 39 20 628.5 0 20 40 25 628.5 0 25 41 18 628.5 0 18 42 61 628.5 0 61 43 37 628.5 0 37 44 43 628.5 0 43 45 45 628.5 0 45 46 47 628.5 0 47 47 45 628.5 0 45 48 28 628.5 0 28 49 63 628.5 0 63 50 19 628.5 0 19 51 56 628.5 0 56 52 338 628.5 0 338 53 102 628.5 0 102 54 122 628.5 0 122 55 110 628.5 0 110 56 30 628.5 0 30 57 17 628.5 0 17 58 19 628.5 0 19 59 116 628.5 0 116 60 76 628.5 0 76 61 195 628.5 0 195 62 579 628.5 0 579 63 158 628.5 0 158 64 355 628.5 0 355 65 501 628.5 0 501 66 756 628.5 0 756 67 1960 628.5 0 1960 68 241 628.5 0 241 69 224 628.5 0 224 70 102 628.5 0 102 71 142 628.5 0 142 72 424 628.5 0 424 73 442 628.5 0 442 74 860 628.5 0 860 75 1243 628.5 0 1243 76 1298 628.5 0 1298 77 3480 628.5 0 3480 78 5456 628.5 0 5456 79 82937 628.5 0 82937 80 30664 628.5 0 30664 81 82513 628.5 0 82513 82 688 628.5 0 688 83 397 628.5 0 397 84 43 628.5 0 43 85 32 628.5 0 32 86 22 628.5 0 22 87 34 628.5 0 34 88 24 628.5 0 24 89 15 628.5 0 15 90 8 628.5 0 8 91 11 628.5 0 11 92 15 628.5 0 15 93 32 628.5 0 32 94 14 628.5 0 14 95 7 628.5 0 7 96 12 628.5 0 12 97 11 628.5 0 11 98 12 628.5 0 12 99 8 628.5 0 8 100 20 628.5 0 20 101 16 628.5 0 16 102 21 628.5 0 21 103 35 628.5 0 35 104 14 628.5 0 14 105 24 628.5 0 24 106 12 628.5 0 12 107 16 628.5 0 16 108 13 628.5 0 13 109 23 628.5 0 23 110 22 628.5 0 22 111 28 628.5 0 28 112 28 628.5 0 28 113 30 628.5 0 30 114 36 628.5 0 36 115 63 628.5 0 63 116 601 628.5 0 601 117 614 628.5 0 614 118 856 628.5 0 856 119 37 628.5 0 37 120 73 628.5 0 73 121 83 628.5 0 83 122 86 628.5 0 86 123 213 628.5 0 213 124 165 628.5 0 165 125 189 628.5 0 189 126 185 628.5 0 185 127 64 628.5 0 64 128 78 628.5 0 78 129 95 628.5 0 95 130 324 628.5 0 324 131 503 628.5 0 503 132 671 628.5 0 671 133 72 628.5 0 72 134 49 628.5 0 49 135 54 628.5 0 54 136 58 628.5 0 58 137 104 628.5 0 104 138 78 628.5 0 78 139 106 628.5 0 106 140 109 628.5 0 109 141 151 628.5 0 151 142 234 628.5 0 234 143 85 628.5 0 85 144 94 628.5 0 94 145 174 628.5 0 174 146 192 628.5 0 192 147 246 628.5 0 246 148 186 628.5 0 186 149 194 628.5 0 194 150 1981 628.5 0 1981 151 2934 628.5 0 2934 152 2476 628.5 0 2476 153 102 628.5 0 102 154 37 628.5 0 37 155 43 628.5 0 43 156 34 628.5 0 34 157 52 628.5 0 52 158 83 628.5 0 83 159 43 628.5 0 43 160 42 628.5 0 42 161 46 628.5 0 46 162 40 628.5 0 40 163 44 628.5 0 44 164 32 628.5 0 32 165 60 628.5 0 60 166 76 628.5 0 76 167 153 628.5 0 153 168 127 628.5 0 127 169 147 628.5 0 147 170 93 628.5 0 93 171 98 628.5 0 98 172 228 628.5 0 228 173 241 628.5 0 241 174 188 628.5 0 188 175 32 628.5 0 32 176 36 628.5 0 36 177 37 628.5 0 37 178 31 628.5 0 31 179 45 628.5 0 45 180 46 628.5 0 46 181 45 628.5 0 45 182 49 628.5 0 49 183 53 628.5 0 53 184 72 628.5 0 72 185 55 628.5 0 55 186 75 628.5 0 75 187 62 628.5 0 62 188 86 628.5 0 86 189 39 628.5 0 39 190 41 628.5 0 41 191 40 628.5 0 40 192 47 628.5 0 47 193 73 628.5 0 73 194 97 628.5 0 97 195 63 628.5 0 63 196 52 628.5 0 52 197 61 628.5 0 61 198 68 628.5 0 68 199 83 628.5 0 83 200 88 628.5 0 88 201 69 628.5 0 69 202 42 628.5 0 42 203 40 628.5 0 40 204 39 628.5 0 39 205 67 628.5 0 67 206 81 628.5 0 81 207 46 628.5 0 46 208 73 628.5 0 73 209 66 628.5 0 66 210 88 628.5 0 88 211 71 628.5 0 71 212 114 628.5 0 114 213 94 628.5 0 94 214 101 628.5 0 101 215 69 628.5 0 69 216 67 628.5 0 67 217 382 628.5 0 382 218 225 628.5 0 225 219 502 628.5 0 502 220 114 628.5 0 114 221 124 628.5 0 124 222 143 628.5 0 143 223 132 628.5 0 132 224 156 628.5 0 156 225 276 628.5 0 276 226 2014 628.5 0 2014 227 1065 628.5 0 1065 228 1734 628.5 0 1734 229 184 628.5 0 184 230 99 628.5 0 99 231 63 628.5 0 63 232 46 628.5 0 46 233 64 628.5 0 64 234 61 628.5 0 61 235 46 628.5 0 46 236 29 628.5 0 29 237 59 628.5 0 59 238 77 628.5 0 77 239 76 628.5 0 76 240 78 628.5 0 78 241 82 628.5 0 82 242 76 628.5 0 76 243 69 628.5 0 69 244 67 628.5 0 67 245 82 628.5 0 82 246 165 628.5 0 165 247 166 628.5 0 166 248 204 628.5 0 204 249 120 628.5 0 120 250 88 628.5 0 88 251 108 628.5 0 108 252 133 628.5 0 133 253 147 628.5 0 147 254 97 628.5 0 97 255 111 628.5 0 111 256 122 628.5 0 122 257 103 628.5 0 103 258 78 628.5 0 78 259 121 628.5 0 121 260 103 628.5 0 103 261 136 628.5 0 136 262 100 628.5 0 100 263 87 628.5 0 87 264 133 628.5 0 133 265 168 628.5 0 168 266 202 628.5 0 202 267 130 628.5 0 130 268 147 628.5 0 147 269 103 628.5 0 103 270 66 628.5 0 66 271 73 628.5 0 73 272 105 628.5 0 105 273 97 628.5 0 97 274 206 628.5 0 206 275 183 628.5 0 183 276 148 628.5 0 148 277 209 628.5 0 209 278 144 628.5 0 144 279 267 628.5 0 267 280 302 628.5 0 302 281 264 628.5 0 264 282 144 628.5 0 144 283 88 628.5 0 88 284 90 628.5 0 90 285 88 628.5 0 88 286 153 628.5 0 153 287 132 628.5 0 132 288 151 628.5 0 151 289 195 628.5 0 195 290 146 628.5 0 146 291 214 628.5 0 214 292 248 628.5 0 248 293 143 628.5 0 143 294 93 628.5 0 93 295 91 628.5 0 91 296 100 628.5 0 100 297 117 628.5 0 117 298 194 628.5 0 194 299 202 628.5 0 202 300 258 628.5 0 258 301 181 628.5 0 181 Finished in 9.18 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 245,497 Reads with adapters: 4,531 (1.8%) Reads that were too short: 471 (0.2%) Reads written (passing filters): 4,060 (1.7%) Total basepairs processed: 53,882,363 bp Total written (filtered): 1,032,510 bp (1.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4531 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 34 0.0 3 0 29 5 17 3366 0.0 3 3024 264 60 18 18 20 0.0 3 2 9 1 8 19 28 0.0 3 7 5 11 5 20 30 0.0 3 4 2 3 21 21 1 0.0 3 0 0 0 1 22 3 0.0 3 0 0 0 3 23 7 0.0 3 0 0 0 7 24 1 0.0 3 0 0 0 1 25 3 0.0 3 0 1 0 2 26 18 0.0 3 1 0 1 16 27 3 0.0 3 0 0 0 3 28 3 0.0 3 0 0 0 3 29 6 0.0 3 0 0 0 6 31 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 202 0.0 3 0 0 1 201 42 1 0.0 3 0 0 0 1 43 2 0.0 3 0 0 0 2 44 39 0.0 3 0 0 1 38 45 2 0.0 3 0 0 0 2 46 2 0.0 3 0 0 0 2 47 11 0.0 3 0 0 0 11 50 4 0.0 3 0 0 0 4 52 2 0.0 3 0 0 0 2 54 1 0.0 3 0 0 0 1 55 16 0.0 3 0 0 0 16 56 6 0.0 3 0 0 0 6 57 7 0.0 3 0 0 0 7 58 6 0.0 3 0 0 0 6 59 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 67 11 0.0 3 0 0 0 11 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 6 0.0 3 0 0 0 6 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 5 0.0 3 0 0 0 5 75 9 0.0 3 0 0 0 9 76 27 0.0 3 0 0 0 27 77 1 0.0 3 0 0 0 1 79 8 0.0 3 0 0 1 7 80 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 84 9 0.0 3 0 0 1 8 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 5 0.0 3 0 0 0 5 94 5 0.0 3 0 0 0 5 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 1 1 104 2 0.0 3 0 0 0 2 105 1 0.0 3 0 0 0 1 106 59 0.0 3 0 0 2 57 107 5 0.0 3 0 0 0 5 108 11 0.0 3 0 0 0 11 109 24 0.0 3 0 0 2 22 110 4 0.0 3 0 0 0 4 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 5 0.0 3 0 0 0 5 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 120 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 8 0.0 3 0 0 0 8 124 3 0.0 3 0 0 0 3 125 3 0.0 3 0 0 0 3 127 5 0.0 3 0 0 0 5 131 1 0.0 3 0 0 0 1 132 7 0.0 3 0 0 0 7 133 32 0.0 3 0 0 0 32 134 5 0.0 3 0 0 1 4 135 7 0.0 3 0 0 0 7 137 2 0.0 3 0 0 0 2 139 3 0.0 3 0 0 0 3 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 142 14 0.0 3 0 0 0 14 143 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 0 3 145 6 0.0 3 0 0 1 5 146 21 0.0 3 0 0 0 21 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 1 149 32 0.0 3 0 0 1 31 150 10 0.0 3 0 0 0 10 151 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 3 0.0 3 0 0 0 3 161 4 0.0 3 0 0 0 4 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 4 0.0 3 0 0 1 3 167 5 0.0 3 0 0 0 5 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 1 171 6 0.0 3 0 0 0 6 172 6 0.0 3 0 0 1 5 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 4 0.0 3 0 0 0 4 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 1 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 207 24 0.0 3 0 0 0 24 208 20 0.0 3 0 0 1 19 209 62 0.0 3 0 0 2 60 210 36 0.0 3 0 0 2 34 211 17 0.0 3 0 0 1 16 212 1 0.0 3 0 0 0 1 213 6 0.0 3 0 0 0 6 215 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 3 0.0 3 0 0 0 3 219 1 0.0 3 0 0 0 1 220 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 4,060 Reads with adapters: 19 (0.5%) Reads written (passing filters): 4,060 (100.0%) Total basepairs processed: 1,032,510 bp Total written (filtered): 1,032,338 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.3% C: 68.4% G: 15.8% T: 10.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 63.4 0 4 4 3 15.9 0 3 5 1 4.0 0 1 13 11 0.0 1 1 10