This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAGAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.38 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 318,763 (12.4%) Reads that were too short: 9,430 (0.4%) Reads written (passing filters): 309,333 (12.0%) Total basepairs processed: 774,927,510 bp Total written (filtered): 68,540,254 bp (8.8%) === Adapter 1 === Sequence: AGAGAC; Type: regular 5'; Length: 6; Trimmed: 318763 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 72 628.5 0 72 7 6308 628.5 0 6308 8 3745 628.5 0 3745 9 19 628.5 0 19 10 28 628.5 0 28 11 65 628.5 0 65 12 13 628.5 0 13 13 5 628.5 0 5 14 4 628.5 0 4 15 6 628.5 0 6 16 5 628.5 0 5 17 10 628.5 0 10 18 9 628.5 0 9 19 11 628.5 0 11 20 17 628.5 0 17 21 44 628.5 0 44 22 22 628.5 0 22 23 14 628.5 0 14 24 7 628.5 0 7 25 11 628.5 0 11 26 16 628.5 0 16 27 28 628.5 0 28 28 77 628.5 0 77 29 17 628.5 0 17 30 21 628.5 0 21 31 11 628.5 0 11 32 10 628.5 0 10 33 19 628.5 0 19 34 20 628.5 0 20 35 41 628.5 0 41 36 40 628.5 0 40 37 6 628.5 0 6 38 10 628.5 0 10 39 25 628.5 0 25 40 24 628.5 0 24 41 22 628.5 0 22 42 72 628.5 0 72 43 41 628.5 0 41 44 45 628.5 0 45 45 55 628.5 0 55 46 55 628.5 0 55 47 37 628.5 0 37 48 17 628.5 0 17 49 76 628.5 0 76 50 26 628.5 0 26 51 56 628.5 0 56 52 459 628.5 0 459 53 109 628.5 0 109 54 67 628.5 0 67 55 93 628.5 0 93 56 18 628.5 0 18 57 21 628.5 0 21 58 16 628.5 0 16 59 132 628.5 0 132 60 98 628.5 0 98 61 222 628.5 0 222 62 604 628.5 0 604 63 112 628.5 0 112 64 284 628.5 0 284 65 281 628.5 0 281 66 467 628.5 0 467 67 839 628.5 0 839 68 284 628.5 0 284 69 271 628.5 0 271 70 105 628.5 0 105 71 160 628.5 0 160 72 483 628.5 0 483 73 516 628.5 0 516 74 963 628.5 0 963 75 1588 628.5 0 1588 76 1656 628.5 0 1656 77 4689 628.5 0 4689 78 6560 628.5 0 6560 79 119117 628.5 0 119117 80 38183 628.5 0 38183 81 107654 628.5 0 107654 82 645 628.5 0 645 83 226 628.5 0 226 84 43 628.5 0 43 85 18 628.5 0 18 86 19 628.5 0 19 87 22 628.5 0 22 88 14 628.5 0 14 89 14 628.5 0 14 90 11 628.5 0 11 91 9 628.5 0 9 92 13 628.5 0 13 93 11 628.5 0 11 94 12 628.5 0 12 95 15 628.5 0 15 96 13 628.5 0 13 97 7 628.5 0 7 98 9 628.5 0 9 99 10 628.5 0 10 100 14 628.5 0 14 101 9 628.5 0 9 102 19 628.5 0 19 103 19 628.5 0 19 104 12 628.5 0 12 105 13 628.5 0 13 106 12 628.5 0 12 107 14 628.5 0 14 108 13 628.5 0 13 109 19 628.5 0 19 110 12 628.5 0 12 111 18 628.5 0 18 112 15 628.5 0 15 113 7 628.5 0 7 114 21 628.5 0 21 115 14 628.5 0 14 116 114 628.5 0 114 117 128 628.5 0 128 118 351 628.5 0 351 119 16 628.5 0 16 120 27 628.5 0 27 121 36 628.5 0 36 122 38 628.5 0 38 123 77 628.5 0 77 124 50 628.5 0 50 125 39 628.5 0 39 126 31 628.5 0 31 127 27 628.5 0 27 128 21 628.5 0 21 129 41 628.5 0 41 130 96 628.5 0 96 131 185 628.5 0 185 132 230 628.5 0 230 133 32 628.5 0 32 134 15 628.5 0 15 135 39 628.5 0 39 136 42 628.5 0 42 137 76 628.5 0 76 138 18 628.5 0 18 139 39 628.5 0 39 140 77 628.5 0 77 141 116 628.5 0 116 142 199 628.5 0 199 143 48 628.5 0 48 144 56 628.5 0 56 145 61 628.5 0 61 146 73 628.5 0 73 147 102 628.5 0 102 148 82 628.5 0 82 149 115 628.5 0 115 150 1756 628.5 0 1756 151 2678 628.5 0 2678 152 2018 628.5 0 2018 153 36 628.5 0 36 154 20 628.5 0 20 155 28 628.5 0 28 156 18 628.5 0 18 157 19 628.5 0 19 158 53 628.5 0 53 159 11 628.5 0 11 160 19 628.5 0 19 161 17 628.5 0 17 162 17 628.5 0 17 163 17 628.5 0 17 164 27 628.5 0 27 165 26 628.5 0 26 166 29 628.5 0 29 167 49 628.5 0 49 168 42 628.5 0 42 169 74 628.5 0 74 170 57 628.5 0 57 171 33 628.5 0 33 172 107 628.5 0 107 173 77 628.5 0 77 174 74 628.5 0 74 175 20 628.5 0 20 176 9 628.5 0 9 177 8 628.5 0 8 178 13 628.5 0 13 179 20 628.5 0 20 180 25 628.5 0 25 181 19 628.5 0 19 182 19 628.5 0 19 183 24 628.5 0 24 184 19 628.5 0 19 185 26 628.5 0 26 186 37 628.5 0 37 187 33 628.5 0 33 188 45 628.5 0 45 189 19 628.5 0 19 190 19 628.5 0 19 191 21 628.5 0 21 192 22 628.5 0 22 193 30 628.5 0 30 194 19 628.5 0 19 195 22 628.5 0 22 196 30 628.5 0 30 197 35 628.5 0 35 198 37 628.5 0 37 199 34 628.5 0 34 200 32 628.5 0 32 201 22 628.5 0 22 202 16 628.5 0 16 203 21 628.5 0 21 204 20 628.5 0 20 205 39 628.5 0 39 206 22 628.5 0 22 207 14 628.5 0 14 208 36 628.5 0 36 209 41 628.5 0 41 210 43 628.5 0 43 211 34 628.5 0 34 212 44 628.5 0 44 213 45 628.5 0 45 214 38 628.5 0 38 215 48 628.5 0 48 216 54 628.5 0 54 217 406 628.5 0 406 218 254 628.5 0 254 219 655 628.5 0 655 220 46 628.5 0 46 221 40 628.5 0 40 222 94 628.5 0 94 223 49 628.5 0 49 224 118 628.5 0 118 225 245 628.5 0 245 226 2010 628.5 0 2010 227 493 628.5 0 493 228 1293 628.5 0 1293 229 46 628.5 0 46 230 34 628.5 0 34 231 22 628.5 0 22 232 35 628.5 0 35 233 23 628.5 0 23 234 31 628.5 0 31 235 24 628.5 0 24 236 19 628.5 0 19 237 18 628.5 0 18 238 29 628.5 0 29 239 30 628.5 0 30 240 36 628.5 0 36 241 33 628.5 0 33 242 30 628.5 0 30 243 30 628.5 0 30 244 30 628.5 0 30 245 34 628.5 0 34 246 45 628.5 0 45 247 41 628.5 0 41 248 52 628.5 0 52 249 28 628.5 0 28 250 36 628.5 0 36 251 40 628.5 0 40 252 28 628.5 0 28 253 45 628.5 0 45 254 25 628.5 0 25 255 29 628.5 0 29 256 39 628.5 0 39 257 39 628.5 0 39 258 34 628.5 0 34 259 22 628.5 0 22 260 30 628.5 0 30 261 23 628.5 0 23 262 38 628.5 0 38 263 21 628.5 0 21 264 32 628.5 0 32 265 31 628.5 0 31 266 55 628.5 0 55 267 30 628.5 0 30 268 34 628.5 0 34 269 33 628.5 0 33 270 28 628.5 0 28 271 25 628.5 0 25 272 34 628.5 0 34 273 53 628.5 0 53 274 110 628.5 0 110 275 153 628.5 0 153 276 52 628.5 0 52 277 180 628.5 0 180 278 37 628.5 0 37 279 59 628.5 0 59 280 53 628.5 0 53 281 40 628.5 0 40 282 31 628.5 0 31 283 25 628.5 0 25 284 57 628.5 0 57 285 39 628.5 0 39 286 88 628.5 0 88 287 46 628.5 0 46 288 40 628.5 0 40 289 51 628.5 0 51 290 60 628.5 0 60 291 68 628.5 0 68 292 81 628.5 0 81 293 44 628.5 0 44 294 35 628.5 0 35 295 29 628.5 0 29 296 36 628.5 0 36 297 40 628.5 0 40 298 68 628.5 0 68 299 58 628.5 0 58 300 88 628.5 0 88 301 70 628.5 0 70 Finished in 13.06 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 309,333 Reads with adapters: 6,704 (2.2%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 6,693 (2.2%) Total basepairs processed: 68,540,254 bp Total written (filtered): 1,823,376 bp (2.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6704 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 36 0.0 3 0 34 1 1 20 3726 0.0 4 3678 46 2 21 32 0.0 4 3 29 22 2887 0.0 4 2857 27 2 1 23 3 0.0 4 0 3 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 64 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 109 1 0.0 4 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 1 120 1 0.0 4 0 0 0 1 129 1 0.0 4 0 0 0 0 1 137 1 0.0 4 1 141 1 0.0 4 1 151 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 1 164 1 0.0 4 0 0 1 171 1 0.0 4 1 203 1 0.0 4 0 1 211 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.24 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 6,693 Reads with adapters: 911 (13.6%) Reads written (passing filters): 6,693 (100.0%) Total basepairs processed: 1,823,376 bp Total written (filtered): 1,802,725 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 911 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 3.8% G: 88.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 104.6 0 3 4 6 26.1 0 6 9 282 0.0 0 9 273 10 207 0.0 1 9 198 11 364 0.0 1 5 359 12 1 0.0 1 0 1 13 1 0.0 1 0 1 150 1 0.0 1 0 1 170 1 0.0 1 1 224 1 0.0 1 1 256 1 0.0 1 1 258 3 0.0 1 3 259 25 0.0 1 24 1 260 5 0.0 1 5 263 9 0.0 1 8 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.