This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.14 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 83,850 (3.3%) Reads that were too short: 3,864 (0.2%) Reads written (passing filters): 79,986 (3.1%) Total basepairs processed: 774,927,510 bp Total written (filtered): 21,486,370 bp (2.8%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 83850 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2343 628.5 0 2343 7 25886 628.5 0 25886 8 811 628.5 0 811 9 19786 628.5 0 19786 10 35 628.5 0 35 11 30 628.5 0 30 12 30 628.5 0 30 13 24 628.5 0 24 14 31 628.5 0 31 15 25 628.5 0 25 16 27 628.5 0 27 17 28 628.5 0 28 18 25 628.5 0 25 19 30 628.5 0 30 20 34 628.5 0 34 21 28 628.5 0 28 22 30 628.5 0 30 23 26 628.5 0 26 24 42 628.5 0 42 25 32 628.5 0 32 26 25 628.5 0 25 27 59 628.5 0 59 28 47 628.5 0 47 29 28 628.5 0 28 30 58 628.5 0 58 31 30 628.5 0 30 32 19 628.5 0 19 33 42 628.5 0 42 34 90 628.5 0 90 35 90 628.5 0 90 36 32 628.5 0 32 37 74 628.5 0 74 38 35 628.5 0 35 39 50 628.5 0 50 40 42 628.5 0 42 41 32 628.5 0 32 42 44 628.5 0 44 43 66 628.5 0 66 44 55 628.5 0 55 45 31 628.5 0 31 46 37 628.5 0 37 47 51 628.5 0 51 48 65 628.5 0 65 49 98 628.5 0 98 50 162 628.5 0 162 51 180 628.5 0 180 52 291 628.5 0 291 53 408 628.5 0 408 54 5791 628.5 0 5791 55 4071 628.5 0 4071 56 10772 628.5 0 10772 57 67 628.5 0 67 58 33 628.5 0 33 59 27 628.5 0 27 60 53 628.5 0 53 61 22 628.5 0 22 62 29 628.5 0 29 63 41 628.5 0 41 64 28 628.5 0 28 65 18 628.5 0 18 66 26 628.5 0 26 67 46 628.5 0 46 68 16 628.5 0 16 69 48 628.5 0 48 70 44 628.5 0 44 71 31 628.5 0 31 72 15 628.5 0 15 73 24 628.5 0 24 74 30 628.5 0 30 75 32 628.5 0 32 76 14 628.5 0 14 77 37 628.5 0 37 78 26 628.5 0 26 79 21 628.5 0 21 80 37 628.5 0 37 81 49 628.5 0 49 82 25 628.5 0 25 83 35 628.5 0 35 84 40 628.5 0 40 85 27 628.5 0 27 86 31 628.5 0 31 87 42 628.5 0 42 88 35 628.5 0 35 89 37 628.5 0 37 90 34 628.5 0 34 91 30 628.5 0 30 92 34 628.5 0 34 93 36 628.5 0 36 94 658 628.5 0 658 95 1100 628.5 0 1100 96 376 628.5 0 376 97 22 628.5 0 22 98 19 628.5 0 19 99 24 628.5 0 24 100 29 628.5 0 29 101 18 628.5 0 18 102 19 628.5 0 19 103 31 628.5 0 31 104 29 628.5 0 29 105 30 628.5 0 30 106 27 628.5 0 27 107 34 628.5 0 34 108 29 628.5 0 29 109 96 628.5 0 96 110 64 628.5 0 64 111 111 628.5 0 111 112 11 628.5 0 11 113 20 628.5 0 20 114 23 628.5 0 23 115 23 628.5 0 23 116 18 628.5 0 18 117 46 628.5 0 46 118 38 628.5 0 38 119 47 628.5 0 47 120 44 628.5 0 44 121 207 628.5 0 207 122 127 628.5 0 127 123 79 628.5 0 79 124 23 628.5 0 23 125 47 628.5 0 47 126 36 628.5 0 36 127 20 628.5 0 20 128 23 628.5 0 23 129 29 628.5 0 29 130 29 628.5 0 29 131 30 628.5 0 30 132 43 628.5 0 43 133 28 628.5 0 28 134 28 628.5 0 28 135 40 628.5 0 40 136 50 628.5 0 50 137 74 628.5 0 74 138 30 628.5 0 30 139 26 628.5 0 26 140 50 628.5 0 50 141 64 628.5 0 64 142 33 628.5 0 33 143 19 628.5 0 19 144 28 628.5 0 28 145 45 628.5 0 45 146 25 628.5 0 25 147 32 628.5 0 32 148 37 628.5 0 37 149 43 628.5 0 43 150 22 628.5 0 22 151 14 628.5 0 14 152 36 628.5 0 36 153 37 628.5 0 37 154 48 628.5 0 48 155 35 628.5 0 35 156 31 628.5 0 31 157 30 628.5 0 30 158 33 628.5 0 33 159 43 628.5 0 43 160 62 628.5 0 62 161 61 628.5 0 61 162 66 628.5 0 66 163 23 628.5 0 23 164 27 628.5 0 27 165 35 628.5 0 35 166 33 628.5 0 33 167 50 628.5 0 50 168 54 628.5 0 54 169 29 628.5 0 29 170 28 628.5 0 28 171 24 628.5 0 24 172 21 628.5 0 21 173 26 628.5 0 26 174 32 628.5 0 32 175 75 628.5 0 75 176 79 628.5 0 79 177 82 628.5 0 82 178 85 628.5 0 85 179 39 628.5 0 39 180 196 628.5 0 196 181 74 628.5 0 74 182 125 628.5 0 125 183 35 628.5 0 35 184 22 628.5 0 22 185 30 628.5 0 30 186 33 628.5 0 33 187 24 628.5 0 24 188 36 628.5 0 36 189 30 628.5 0 30 190 20 628.5 0 20 191 44 628.5 0 44 192 30 628.5 0 30 193 52 628.5 0 52 194 43 628.5 0 43 195 32 628.5 0 32 196 30 628.5 0 30 197 21 628.5 0 21 198 40 628.5 0 40 199 33 628.5 0 33 200 21 628.5 0 21 201 26 628.5 0 26 202 31 628.5 0 31 203 39 628.5 0 39 204 43 628.5 0 43 205 47 628.5 0 47 206 36 628.5 0 36 207 34 628.5 0 34 208 27 628.5 0 27 209 31 628.5 0 31 210 25 628.5 0 25 211 23 628.5 0 23 212 31 628.5 0 31 213 35 628.5 0 35 214 22 628.5 0 22 215 26 628.5 0 26 216 26 628.5 0 26 217 22 628.5 0 22 218 34 628.5 0 34 219 37 628.5 0 37 220 33 628.5 0 33 221 38 628.5 0 38 222 45 628.5 0 45 223 43 628.5 0 43 224 32 628.5 0 32 225 37 628.5 0 37 226 43 628.5 0 43 227 38 628.5 0 38 228 41 628.5 0 41 229 37 628.5 0 37 230 39 628.5 0 39 231 28 628.5 0 28 232 21 628.5 0 21 233 26 628.5 0 26 234 19 628.5 0 19 235 28 628.5 0 28 236 37 628.5 0 37 237 42 628.5 0 42 238 66 628.5 0 66 239 34 628.5 0 34 240 38 628.5 0 38 241 22 628.5 0 22 242 25 628.5 0 25 243 29 628.5 0 29 244 20 628.5 0 20 245 29 628.5 0 29 246 24 628.5 0 24 247 49 628.5 0 49 248 27 628.5 0 27 249 35 628.5 0 35 250 20 628.5 0 20 251 22 628.5 0 22 252 31 628.5 0 31 253 42 628.5 0 42 254 40 628.5 0 40 255 39 628.5 0 39 256 30 628.5 0 30 257 34 628.5 0 34 258 43 628.5 0 43 259 26 628.5 0 26 260 36 628.5 0 36 261 39 628.5 0 39 262 75 628.5 0 75 263 56 628.5 0 56 264 76 628.5 0 76 265 24 628.5 0 24 266 37 628.5 0 37 267 33 628.5 0 33 268 30 628.5 0 30 269 36 628.5 0 36 270 26 628.5 0 26 271 39 628.5 0 39 272 28 628.5 0 28 273 57 628.5 0 57 274 63 628.5 0 63 275 75 628.5 0 75 276 34 628.5 0 34 277 26 628.5 0 26 278 59 628.5 0 59 279 93 628.5 0 93 280 101 628.5 0 101 281 39 628.5 0 39 282 41 628.5 0 41 283 49 628.5 0 49 284 41 628.5 0 41 285 38 628.5 0 38 286 30 628.5 0 30 287 37 628.5 0 37 288 37 628.5 0 37 289 26 628.5 0 26 290 37 628.5 0 37 291 57 628.5 0 57 292 74 628.5 0 74 293 34 628.5 0 34 294 41 628.5 0 41 295 36 628.5 0 36 296 43 628.5 0 43 297 31 628.5 0 31 298 25 628.5 0 25 299 49 628.5 0 49 300 85 628.5 0 85 301 50 628.5 0 50 Finished in 4.18 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 79,986 Reads with adapters: 24,580 (30.7%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 24,577 (30.7%) Total basepairs processed: 21,486,370 bp Total written (filtered): 6,628,320 bp (30.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24580 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 6 0.0 3 0 0 6 19 247 0.0 3 0 241 6 20 23244 0.0 4 22869 363 12 21 65 0.0 4 7 56 1 1 22 10 0.0 4 5 2 1 0 2 23 17 0.0 4 0 0 0 17 24 1 0.0 4 1 26 1 0.0 4 1 28 1 0.0 4 1 113 1 0.0 4 0 0 0 1 120 1 0.0 4 1 126 15 0.0 4 0 0 0 0 15 127 898 0.0 4 0 0 0 0 898 128 5 0.0 4 0 0 0 0 5 129 64 0.0 4 0 0 0 0 64 132 1 0.0 4 0 0 0 1 153 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.83 s (34 us/read; 1.78 M reads/minute). === Summary === Total reads processed: 24,577 Reads with adapters: 350 (1.4%) Reads written (passing filters): 24,577 (100.0%) Total basepairs processed: 6,628,320 bp Total written (filtered): 6,624,521 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 350 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.9% C: 5.7% G: 78.9% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 47 384.0 0 47 4 6 96.0 0 6 5 3 24.0 0 3 6 1 6.0 0 1 9 1 0.1 0 0 1 11 212 0.0 1 3 209 12 73 0.0 1 0 73 13 1 0.0 1 0 1 15 4 0.0 1 0 4 152 1 0.0 1 0 1 171 1 0.0 1 0 1