This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.34 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 207,361 (8.1%) Reads that were too short: 14,277 (0.6%) Reads written (passing filters): 193,084 (7.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 52,629,963 bp (6.8%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 207361 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1167 628.5 0 1167 7 2398 628.5 0 2398 8 81548 628.5 0 81548 9 68597 628.5 0 68597 10 121 628.5 0 121 11 83 628.5 0 83 12 55 628.5 0 55 13 55 628.5 0 55 14 65 628.5 0 65 15 50 628.5 0 50 16 60 628.5 0 60 17 54 628.5 0 54 18 49 628.5 0 49 19 40 628.5 0 40 20 56 628.5 0 56 21 43 628.5 0 43 22 53 628.5 0 53 23 73 628.5 0 73 24 50 628.5 0 50 25 52 628.5 0 52 26 57 628.5 0 57 27 65 628.5 0 65 28 65 628.5 0 65 29 56 628.5 0 56 30 48 628.5 0 48 31 90 628.5 0 90 32 141 628.5 0 141 33 54 628.5 0 54 34 50 628.5 0 50 35 59 628.5 0 59 36 58 628.5 0 58 37 55 628.5 0 55 38 73 628.5 0 73 39 50 628.5 0 50 40 56 628.5 0 56 41 106 628.5 0 106 42 65 628.5 0 65 43 71 628.5 0 71 44 64 628.5 0 64 45 183 628.5 0 183 46 91 628.5 0 91 47 144 628.5 0 144 48 47 628.5 0 47 49 68 628.5 0 68 50 46 628.5 0 46 51 50 628.5 0 50 52 43 628.5 0 43 53 53 628.5 0 53 54 49 628.5 0 49 55 50 628.5 0 50 56 76 628.5 0 76 57 50 628.5 0 50 58 69 628.5 0 69 59 75 628.5 0 75 60 70 628.5 0 70 61 63 628.5 0 63 62 103 628.5 0 103 63 70 628.5 0 70 64 55 628.5 0 55 65 119 628.5 0 119 66 183 628.5 0 183 67 87 628.5 0 87 68 103 628.5 0 103 69 129 628.5 0 129 70 118 628.5 0 118 71 149 628.5 0 149 72 308 628.5 0 308 73 218 628.5 0 218 74 153 628.5 0 153 75 182 628.5 0 182 76 203 628.5 0 203 77 219 628.5 0 219 78 284 628.5 0 284 79 5186 628.5 0 5186 80 6318 628.5 0 6318 81 4187 628.5 0 4187 82 152 628.5 0 152 83 204 628.5 0 204 84 179 628.5 0 179 85 62 628.5 0 62 86 46 628.5 0 46 87 42 628.5 0 42 88 59 628.5 0 59 89 55 628.5 0 55 90 51 628.5 0 51 91 61 628.5 0 61 92 77 628.5 0 77 93 68 628.5 0 68 94 54 628.5 0 54 95 75 628.5 0 75 96 65 628.5 0 65 97 80 628.5 0 80 98 137 628.5 0 137 99 155 628.5 0 155 100 101 628.5 0 101 101 69 628.5 0 69 102 76 628.5 0 76 103 79 628.5 0 79 104 97 628.5 0 97 105 83 628.5 0 83 106 68 628.5 0 68 107 84 628.5 0 84 108 102 628.5 0 102 109 78 628.5 0 78 110 75 628.5 0 75 111 92 628.5 0 92 112 99 628.5 0 99 113 71 628.5 0 71 114 71 628.5 0 71 115 83 628.5 0 83 116 78 628.5 0 78 117 102 628.5 0 102 118 80 628.5 0 80 119 86 628.5 0 86 120 99 628.5 0 99 121 132 628.5 0 132 122 109 628.5 0 109 123 123 628.5 0 123 124 164 628.5 0 164 125 324 628.5 0 324 126 311 628.5 0 311 127 304 628.5 0 304 128 184 628.5 0 184 129 219 628.5 0 219 130 259 628.5 0 259 131 115 628.5 0 115 132 81 628.5 0 81 133 72 628.5 0 72 134 112 628.5 0 112 135 109 628.5 0 109 136 121 628.5 0 121 137 167 628.5 0 167 138 133 628.5 0 133 139 290 628.5 0 290 140 188 628.5 0 188 141 619 628.5 0 619 142 204 628.5 0 204 143 236 628.5 0 236 144 105 628.5 0 105 145 82 628.5 0 82 146 86 628.5 0 86 147 123 628.5 0 123 148 80 628.5 0 80 149 63 628.5 0 63 150 120 628.5 0 120 151 129 628.5 0 129 152 161 628.5 0 161 153 81 628.5 0 81 154 72 628.5 0 72 155 81 628.5 0 81 156 78 628.5 0 78 157 81 628.5 0 81 158 165 628.5 0 165 159 220 628.5 0 220 160 201 628.5 0 201 161 90 628.5 0 90 162 75 628.5 0 75 163 118 628.5 0 118 164 231 628.5 0 231 165 220 628.5 0 220 166 155 628.5 0 155 167 77 628.5 0 77 168 77 628.5 0 77 169 77 628.5 0 77 170 96 628.5 0 96 171 90 628.5 0 90 172 63 628.5 0 63 173 67 628.5 0 67 174 69 628.5 0 69 175 57 628.5 0 57 176 57 628.5 0 57 177 118 628.5 0 118 178 109 628.5 0 109 179 132 628.5 0 132 180 143 628.5 0 143 181 176 628.5 0 176 182 1883 628.5 0 1883 183 1010 628.5 0 1010 184 1290 628.5 0 1290 185 77 628.5 0 77 186 58 628.5 0 58 187 52 628.5 0 52 188 63 628.5 0 63 189 72 628.5 0 72 190 64 628.5 0 64 191 81 628.5 0 81 192 61 628.5 0 61 193 76 628.5 0 76 194 73 628.5 0 73 195 76 628.5 0 76 196 60 628.5 0 60 197 58 628.5 0 58 198 71 628.5 0 71 199 56 628.5 0 56 200 73 628.5 0 73 201 81 628.5 0 81 202 72 628.5 0 72 203 83 628.5 0 83 204 86 628.5 0 86 205 99 628.5 0 99 206 157 628.5 0 157 207 219 628.5 0 219 208 138 628.5 0 138 209 134 628.5 0 134 210 94 628.5 0 94 211 116 628.5 0 116 212 150 628.5 0 150 213 175 628.5 0 175 214 108 628.5 0 108 215 98 628.5 0 98 216 87 628.5 0 87 217 79 628.5 0 79 218 116 628.5 0 116 219 89 628.5 0 89 220 125 628.5 0 125 221 119 628.5 0 119 222 97 628.5 0 97 223 131 628.5 0 131 224 147 628.5 0 147 225 146 628.5 0 146 226 162 628.5 0 162 227 153 628.5 0 153 228 140 628.5 0 140 229 68 628.5 0 68 230 86 628.5 0 86 231 135 628.5 0 135 232 145 628.5 0 145 233 151 628.5 0 151 234 90 628.5 0 90 235 90 628.5 0 90 236 103 628.5 0 103 237 106 628.5 0 106 238 104 628.5 0 104 239 123 628.5 0 123 240 85 628.5 0 85 241 110 628.5 0 110 242 95 628.5 0 95 243 70 628.5 0 70 244 64 628.5 0 64 245 69 628.5 0 69 246 77 628.5 0 77 247 94 628.5 0 94 248 85 628.5 0 85 249 87 628.5 0 87 250 100 628.5 0 100 251 104 628.5 0 104 252 98 628.5 0 98 253 239 628.5 0 239 254 502 628.5 0 502 255 732 628.5 0 732 256 218 628.5 0 218 257 110 628.5 0 110 258 73 628.5 0 73 259 103 628.5 0 103 260 220 628.5 0 220 261 291 628.5 0 291 262 171 628.5 0 171 263 161 628.5 0 161 264 375 628.5 0 375 265 408 628.5 0 408 266 1532 628.5 0 1532 267 87 628.5 0 87 268 81 628.5 0 81 269 93 628.5 0 93 270 122 628.5 0 122 271 92 628.5 0 92 272 89 628.5 0 89 273 108 628.5 0 108 274 98 628.5 0 98 275 130 628.5 0 130 276 158 628.5 0 158 277 149 628.5 0 149 278 119 628.5 0 119 279 89 628.5 0 89 280 85 628.5 0 85 281 84 628.5 0 84 282 110 628.5 0 110 283 76 628.5 0 76 284 87 628.5 0 87 285 73 628.5 0 73 286 83 628.5 0 83 287 83 628.5 0 83 288 96 628.5 0 96 289 167 628.5 0 167 290 114 628.5 0 114 291 94 628.5 0 94 292 96 628.5 0 96 293 89 628.5 0 89 294 78 628.5 0 78 295 85 628.5 0 85 296 86 628.5 0 86 297 85 628.5 0 85 298 98 628.5 0 98 299 81 628.5 0 81 300 98 628.5 0 98 301 120 628.5 0 120 Finished in 8.43 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 193,084 Reads with adapters: 80,658 (41.8%) Reads that were too short: 268 (0.1%) Reads written (passing filters): 80,390 (41.6%) Total basepairs processed: 52,629,963 bp Total written (filtered): 22,151,846 bp (42.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 80658 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 23 0.0 2 0 0 0 23 15 97 0.0 3 0 0 55 42 16 876 0.0 3 0 686 121 69 17 78303 0.0 3 70491 6075 1371 366 18 193 0.0 3 11 131 28 23 19 445 0.0 3 2 3 252 188 20 189 0.0 3 1 0 0 188 21 30 0.0 3 1 0 3 26 24 2 0.0 3 0 0 1 1 25 13 0.0 3 0 0 1 12 28 29 0.0 3 0 0 0 29 29 1 0.0 3 0 0 0 1 31 7 0.0 3 0 0 1 6 32 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 10 0.0 3 0 0 0 10 42 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 0 5 45 2 0.0 3 0 0 0 2 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 6 0.0 3 0 0 1 5 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 3 0.0 3 0 0 0 3 54 2 0.0 3 0 0 0 2 55 4 0.0 3 0 0 0 4 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 1 58 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 8 0.0 3 0 0 0 8 68 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 1 1 96 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 1 3 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 8 0.0 3 0 0 0 8 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 47 0.0 3 0 0 0 47 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 18 0.0 3 0 0 2 16 122 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 134 14 0.0 3 0 0 0 14 135 3 0.0 3 0 0 0 3 136 18 0.0 3 0 0 0 18 137 1 0.0 3 0 0 0 1 138 5 0.0 3 0 0 0 5 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 1 0.0 3 0 0 0 1 145 3 0.0 3 0 0 0 3 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 150 3 0.0 3 0 0 0 3 151 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 163 5 0.0 3 0 0 0 5 164 2 0.0 3 0 0 1 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 40 0.0 3 0 0 0 40 173 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 175 7 0.0 3 0 0 0 7 185 6 0.0 3 0 0 0 6 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 192 4 0.0 3 0 0 0 4 194 11 0.0 3 0 0 1 10 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 1 202 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 1 205 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 213 16 0.0 3 0 0 1 15 214 45 0.0 3 0 1 2 42 215 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 3 0.0 3 0 0 0 3 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 7 0.0 3 0 0 1 6 281 8 0.0 3 0 0 0 8 282 7 0.0 3 0 0 0 7 283 3 0.0 3 0 0 1 2 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.80 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 80,390 Reads with adapters: 489 (0.6%) Reads written (passing filters): 80,390 (100.0%) Total basepairs processed: 22,151,846 bp Total written (filtered): 22,150,203 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 489 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.3% C: 12.9% G: 32.5% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 343 1256.1 0 343 4 129 314.0 0 129 5 14 78.5 0 14 6 1 19.6 0 1 11 2 0.0 1 0 2