This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.76 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 223,362 (8.7%) Reads that were too short: 15,388 (0.6%) Reads written (passing filters): 207,974 (8.1%) Total basepairs processed: 774,927,510 bp Total written (filtered): 56,514,296 bp (7.3%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 223362 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 849 628.5 0 849 7 1625 628.5 0 1625 8 78518 628.5 0 78518 9 81604 628.5 0 81604 10 147 628.5 0 147 11 107 628.5 0 107 12 73 628.5 0 73 13 98 628.5 0 98 14 113 628.5 0 113 15 56 628.5 0 56 16 69 628.5 0 69 17 70 628.5 0 70 18 77 628.5 0 77 19 63 628.5 0 63 20 66 628.5 0 66 21 77 628.5 0 77 22 92 628.5 0 92 23 80 628.5 0 80 24 89 628.5 0 89 25 94 628.5 0 94 26 95 628.5 0 95 27 95 628.5 0 95 28 98 628.5 0 98 29 108 628.5 0 108 30 80 628.5 0 80 31 95 628.5 0 95 32 127 628.5 0 127 33 69 628.5 0 69 34 82 628.5 0 82 35 109 628.5 0 109 36 79 628.5 0 79 37 79 628.5 0 79 38 101 628.5 0 101 39 84 628.5 0 84 40 88 628.5 0 88 41 124 628.5 0 124 42 70 628.5 0 70 43 82 628.5 0 82 44 101 628.5 0 101 45 197 628.5 0 197 46 117 628.5 0 117 47 149 628.5 0 149 48 93 628.5 0 93 49 117 628.5 0 117 50 103 628.5 0 103 51 76 628.5 0 76 52 84 628.5 0 84 53 91 628.5 0 91 54 82 628.5 0 82 55 89 628.5 0 89 56 107 628.5 0 107 57 123 628.5 0 123 58 102 628.5 0 102 59 98 628.5 0 98 60 94 628.5 0 94 61 88 628.5 0 88 62 112 628.5 0 112 63 106 628.5 0 106 64 128 628.5 0 128 65 169 628.5 0 169 66 218 628.5 0 218 67 110 628.5 0 110 68 139 628.5 0 139 69 158 628.5 0 158 70 136 628.5 0 136 71 182 628.5 0 182 72 204 628.5 0 204 73 262 628.5 0 262 74 159 628.5 0 159 75 175 628.5 0 175 76 249 628.5 0 249 77 265 628.5 0 265 78 289 628.5 0 289 79 5559 628.5 0 5559 80 7048 628.5 0 7048 81 4770 628.5 0 4770 82 187 628.5 0 187 83 190 628.5 0 190 84 176 628.5 0 176 85 109 628.5 0 109 86 113 628.5 0 113 87 109 628.5 0 109 88 137 628.5 0 137 89 119 628.5 0 119 90 113 628.5 0 113 91 109 628.5 0 109 92 125 628.5 0 125 93 110 628.5 0 110 94 119 628.5 0 119 95 126 628.5 0 126 96 125 628.5 0 125 97 161 628.5 0 161 98 123 628.5 0 123 99 130 628.5 0 130 100 136 628.5 0 136 101 123 628.5 0 123 102 117 628.5 0 117 103 118 628.5 0 118 104 124 628.5 0 124 105 134 628.5 0 134 106 118 628.5 0 118 107 155 628.5 0 155 108 173 628.5 0 173 109 137 628.5 0 137 110 96 628.5 0 96 111 129 628.5 0 129 112 141 628.5 0 141 113 119 628.5 0 119 114 104 628.5 0 104 115 118 628.5 0 118 116 132 628.5 0 132 117 163 628.5 0 163 118 100 628.5 0 100 119 124 628.5 0 124 120 127 628.5 0 127 121 123 628.5 0 123 122 156 628.5 0 156 123 130 628.5 0 130 124 196 628.5 0 196 125 210 628.5 0 210 126 235 628.5 0 235 127 213 628.5 0 213 128 198 628.5 0 198 129 228 628.5 0 228 130 241 628.5 0 241 131 143 628.5 0 143 132 119 628.5 0 119 133 103 628.5 0 103 134 126 628.5 0 126 135 108 628.5 0 108 136 143 628.5 0 143 137 155 628.5 0 155 138 172 628.5 0 172 139 233 628.5 0 233 140 188 628.5 0 188 141 614 628.5 0 614 142 240 628.5 0 240 143 248 628.5 0 248 144 150 628.5 0 150 145 102 628.5 0 102 146 128 628.5 0 128 147 187 628.5 0 187 148 102 628.5 0 102 149 104 628.5 0 104 150 123 628.5 0 123 151 157 628.5 0 157 152 161 628.5 0 161 153 114 628.5 0 114 154 110 628.5 0 110 155 125 628.5 0 125 156 109 628.5 0 109 157 122 628.5 0 122 158 254 628.5 0 254 159 271 628.5 0 271 160 235 628.5 0 235 161 125 628.5 0 125 162 114 628.5 0 114 163 103 628.5 0 103 164 316 628.5 0 316 165 318 628.5 0 318 166 217 628.5 0 217 167 100 628.5 0 100 168 126 628.5 0 126 169 120 628.5 0 120 170 125 628.5 0 125 171 134 628.5 0 134 172 107 628.5 0 107 173 106 628.5 0 106 174 136 628.5 0 136 175 120 628.5 0 120 176 83 628.5 0 83 177 142 628.5 0 142 178 143 628.5 0 143 179 166 628.5 0 166 180 143 628.5 0 143 181 199 628.5 0 199 182 1279 628.5 0 1279 183 637 628.5 0 637 184 829 628.5 0 829 185 104 628.5 0 104 186 83 628.5 0 83 187 102 628.5 0 102 188 110 628.5 0 110 189 114 628.5 0 114 190 104 628.5 0 104 191 105 628.5 0 105 192 115 628.5 0 115 193 109 628.5 0 109 194 137 628.5 0 137 195 129 628.5 0 129 196 112 628.5 0 112 197 98 628.5 0 98 198 118 628.5 0 118 199 134 628.5 0 134 200 137 628.5 0 137 201 149 628.5 0 149 202 120 628.5 0 120 203 130 628.5 0 130 204 127 628.5 0 127 205 130 628.5 0 130 206 142 628.5 0 142 207 150 628.5 0 150 208 200 628.5 0 200 209 150 628.5 0 150 210 125 628.5 0 125 211 151 628.5 0 151 212 152 628.5 0 152 213 152 628.5 0 152 214 91 628.5 0 91 215 126 628.5 0 126 216 131 628.5 0 131 217 109 628.5 0 109 218 130 628.5 0 130 219 108 628.5 0 108 220 167 628.5 0 167 221 152 628.5 0 152 222 122 628.5 0 122 223 130 628.5 0 130 224 164 628.5 0 164 225 153 628.5 0 153 226 149 628.5 0 149 227 151 628.5 0 151 228 137 628.5 0 137 229 129 628.5 0 129 230 117 628.5 0 117 231 179 628.5 0 179 232 115 628.5 0 115 233 163 628.5 0 163 234 127 628.5 0 127 235 142 628.5 0 142 236 119 628.5 0 119 237 142 628.5 0 142 238 165 628.5 0 165 239 141 628.5 0 141 240 127 628.5 0 127 241 135 628.5 0 135 242 140 628.5 0 140 243 122 628.5 0 122 244 121 628.5 0 121 245 109 628.5 0 109 246 105 628.5 0 105 247 137 628.5 0 137 248 156 628.5 0 156 249 134 628.5 0 134 250 136 628.5 0 136 251 138 628.5 0 138 252 166 628.5 0 166 253 390 628.5 0 390 254 791 628.5 0 791 255 1143 628.5 0 1143 256 276 628.5 0 276 257 148 628.5 0 148 258 121 628.5 0 121 259 127 628.5 0 127 260 188 628.5 0 188 261 223 628.5 0 223 262 129 628.5 0 129 263 105 628.5 0 105 264 165 628.5 0 165 265 169 628.5 0 169 266 417 628.5 0 417 267 99 628.5 0 99 268 111 628.5 0 111 269 102 628.5 0 102 270 88 628.5 0 88 271 122 628.5 0 122 272 115 628.5 0 115 273 115 628.5 0 115 274 135 628.5 0 135 275 150 628.5 0 150 276 136 628.5 0 136 277 109 628.5 0 109 278 115 628.5 0 115 279 103 628.5 0 103 280 131 628.5 0 131 281 110 628.5 0 110 282 110 628.5 0 110 283 112 628.5 0 112 284 144 628.5 0 144 285 107 628.5 0 107 286 108 628.5 0 108 287 103 628.5 0 103 288 103 628.5 0 103 289 145 628.5 0 145 290 102 628.5 0 102 291 97 628.5 0 97 292 108 628.5 0 108 293 115 628.5 0 115 294 114 628.5 0 114 295 100 628.5 0 100 296 97 628.5 0 97 297 123 628.5 0 123 298 118 628.5 0 118 299 118 628.5 0 118 300 96 628.5 0 96 301 121 628.5 0 121 Finished in 9.97 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 207,974 Reads with adapters: 86,751 (41.7%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 86,722 (41.7%) Total basepairs processed: 56,514,296 bp Total written (filtered): 23,426,783 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 86751 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 23 0.0 3 0 0 23 19 889 0.0 3 0 875 14 20 83997 0.0 4 82703 1248 37 9 21 167 0.0 4 35 127 5 22 15 0.0 4 3 1 8 2 1 23 57 0.0 4 1 0 0 54 2 24 4 0.0 4 1 0 0 0 3 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 78 1 0.0 4 1 83 1 0.0 4 1 86 1 0.0 4 1 114 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 25 0.0 4 0 0 0 0 25 127 1523 0.0 4 0 0 0 0 1523 128 4 0.0 4 0 0 0 0 4 130 3 0.0 4 0 0 0 0 3 146 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 1 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 1 183 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 1 229 1 0.0 4 0 0 0 0 1 235 4 0.0 4 0 0 0 0 4 242 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.06 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 86,722 Reads with adapters: 10,674 (12.3%) Reads written (passing filters): 86,722 (100.0%) Total basepairs processed: 23,426,783 bp Total written (filtered): 23,278,826 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10674 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 4.7% G: 92.9% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 17 1355.0 0 17 4 13 338.8 0 13 5 12 84.7 0 12 6 4 21.2 0 4 7 2 5.3 0 2 8 1 1.3 0 1 9 3764 0.3 0 126 3638 10 5724 0.1 1 213 5511 11 915 0.0 1 4 911 12 9 0.0 1 0 9 13 4 0.0 1 0 4 36 1 0.0 1 1 150 2 0.0 1 0 2 151 1 0.0 1 0 1 169 2 0.0 1 2 185 1 0.0 1 0 1 189 1 0.0 1 0 1 196 1 0.0 1 1 208 1 0.0 1 0 1 210 1 0.0 1 1 223 177 0.0 1 171 6 224 8 0.0 1 8 225 1 0.0 1 1 257 1 0.0 1 1 259 11 0.0 1 11 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.