This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.12 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 60,495 (2.3%) Reads that were too short: 11,803 (0.5%) Reads written (passing filters): 48,692 (1.9%) Total basepairs processed: 774,927,510 bp Total written (filtered): 12,684,450 bp (1.6%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 60495 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 688 628.5 0 688 7 17283 628.5 0 17283 8 15691 628.5 0 15691 9 69 628.5 0 69 10 47 628.5 0 47 11 57 628.5 0 57 12 40 628.5 0 40 13 63 628.5 0 63 14 49 628.5 0 49 15 54 628.5 0 54 16 101 628.5 0 101 17 54 628.5 0 54 18 67 628.5 0 67 19 58 628.5 0 58 20 38 628.5 0 38 21 33 628.5 0 33 22 46 628.5 0 46 23 50 628.5 0 50 24 51 628.5 0 51 25 61 628.5 0 61 26 111 628.5 0 111 27 88 628.5 0 88 28 273 628.5 0 273 29 34 628.5 0 34 30 41 628.5 0 41 31 46 628.5 0 46 32 58 628.5 0 58 33 41 628.5 0 41 34 32 628.5 0 32 35 45 628.5 0 45 36 126 628.5 0 126 37 65 628.5 0 65 38 36 628.5 0 36 39 53 628.5 0 53 40 39 628.5 0 39 41 43 628.5 0 43 42 58 628.5 0 58 43 44 628.5 0 44 44 41 628.5 0 41 45 38 628.5 0 38 46 48 628.5 0 48 47 46 628.5 0 46 48 41 628.5 0 41 49 41 628.5 0 41 50 44 628.5 0 44 51 60 628.5 0 60 52 35 628.5 0 35 53 33 628.5 0 33 54 48 628.5 0 48 55 35 628.5 0 35 56 35 628.5 0 35 57 32 628.5 0 32 58 38 628.5 0 38 59 46 628.5 0 46 60 39 628.5 0 39 61 46 628.5 0 46 62 40 628.5 0 40 63 45 628.5 0 45 64 43 628.5 0 43 65 53 628.5 0 53 66 49 628.5 0 49 67 49 628.5 0 49 68 45 628.5 0 45 69 49 628.5 0 49 70 36 628.5 0 36 71 40 628.5 0 40 72 44 628.5 0 44 73 38 628.5 0 38 74 48 628.5 0 48 75 63 628.5 0 63 76 51 628.5 0 51 77 75 628.5 0 75 78 48 628.5 0 48 79 49 628.5 0 49 80 46 628.5 0 46 81 59 628.5 0 59 82 61 628.5 0 61 83 50 628.5 0 50 84 49 628.5 0 49 85 53 628.5 0 53 86 43 628.5 0 43 87 45 628.5 0 45 88 25 628.5 0 25 89 43 628.5 0 43 90 42 628.5 0 42 91 60 628.5 0 60 92 59 628.5 0 59 93 32 628.5 0 32 94 57 628.5 0 57 95 53 628.5 0 53 96 66 628.5 0 66 97 54 628.5 0 54 98 80 628.5 0 80 99 84 628.5 0 84 100 101 628.5 0 101 101 64 628.5 0 64 102 68 628.5 0 68 103 60 628.5 0 60 104 48 628.5 0 48 105 56 628.5 0 56 106 80 628.5 0 80 107 73 628.5 0 73 108 310 628.5 0 310 109 391 628.5 0 391 110 1072 628.5 0 1072 111 116 628.5 0 116 112 103 628.5 0 103 113 86 628.5 0 86 114 178 628.5 0 178 115 90 628.5 0 90 116 144 628.5 0 144 117 69 628.5 0 69 118 42 628.5 0 42 119 67 628.5 0 67 120 75 628.5 0 75 121 47 628.5 0 47 122 91 628.5 0 91 123 76 628.5 0 76 124 57 628.5 0 57 125 59 628.5 0 59 126 61 628.5 0 61 127 52 628.5 0 52 128 62 628.5 0 62 129 95 628.5 0 95 130 71 628.5 0 71 131 113 628.5 0 113 132 76 628.5 0 76 133 80 628.5 0 80 134 85 628.5 0 85 135 89 628.5 0 89 136 109 628.5 0 109 137 84 628.5 0 84 138 84 628.5 0 84 139 114 628.5 0 114 140 164 628.5 0 164 141 140 628.5 0 140 142 115 628.5 0 115 143 99 628.5 0 99 144 90 628.5 0 90 145 126 628.5 0 126 146 76 628.5 0 76 147 73 628.5 0 73 148 71 628.5 0 71 149 66 628.5 0 66 150 76 628.5 0 76 151 48 628.5 0 48 152 56 628.5 0 56 153 71 628.5 0 71 154 61 628.5 0 61 155 145 628.5 0 145 156 69 628.5 0 69 157 120 628.5 0 120 158 119 628.5 0 119 159 78 628.5 0 78 160 87 628.5 0 87 161 78 628.5 0 78 162 86 628.5 0 86 163 186 628.5 0 186 164 99 628.5 0 99 165 147 628.5 0 147 166 100 628.5 0 100 167 119 628.5 0 119 168 62 628.5 0 62 169 95 628.5 0 95 170 81 628.5 0 81 171 70 628.5 0 70 172 81 628.5 0 81 173 68 628.5 0 68 174 50 628.5 0 50 175 49 628.5 0 49 176 83 628.5 0 83 177 57 628.5 0 57 178 110 628.5 0 110 179 86 628.5 0 86 180 97 628.5 0 97 181 64 628.5 0 64 182 101 628.5 0 101 183 72 628.5 0 72 184 82 628.5 0 82 185 59 628.5 0 59 186 75 628.5 0 75 187 83 628.5 0 83 188 78 628.5 0 78 189 64 628.5 0 64 190 103 628.5 0 103 191 77 628.5 0 77 192 87 628.5 0 87 193 67 628.5 0 67 194 61 628.5 0 61 195 100 628.5 0 100 196 69 628.5 0 69 197 69 628.5 0 69 198 65 628.5 0 65 199 67 628.5 0 67 200 76 628.5 0 76 201 116 628.5 0 116 202 115 628.5 0 115 203 102 628.5 0 102 204 117 628.5 0 117 205 86 628.5 0 86 206 85 628.5 0 85 207 89 628.5 0 89 208 145 628.5 0 145 209 146 628.5 0 146 210 230 628.5 0 230 211 130 628.5 0 130 212 71 628.5 0 71 213 83 628.5 0 83 214 67 628.5 0 67 215 80 628.5 0 80 216 102 628.5 0 102 217 80 628.5 0 80 218 64 628.5 0 64 219 74 628.5 0 74 220 86 628.5 0 86 221 73 628.5 0 73 222 117 628.5 0 117 223 218 628.5 0 218 224 276 628.5 0 276 225 126 628.5 0 126 226 154 628.5 0 154 227 74 628.5 0 74 228 46 628.5 0 46 229 63 628.5 0 63 230 51 628.5 0 51 231 48 628.5 0 48 232 76 628.5 0 76 233 60 628.5 0 60 234 82 628.5 0 82 235 57 628.5 0 57 236 56 628.5 0 56 237 71 628.5 0 71 238 75 628.5 0 75 239 65 628.5 0 65 240 97 628.5 0 97 241 80 628.5 0 80 242 96 628.5 0 96 243 167 628.5 0 167 244 251 628.5 0 251 245 164 628.5 0 164 246 218 628.5 0 218 247 73 628.5 0 73 248 68 628.5 0 68 249 56 628.5 0 56 250 59 628.5 0 59 251 74 628.5 0 74 252 100 628.5 0 100 253 262 628.5 0 262 254 250 628.5 0 250 255 488 628.5 0 488 256 104 628.5 0 104 257 77 628.5 0 77 258 305 628.5 0 305 259 324 628.5 0 324 260 430 628.5 0 430 261 55 628.5 0 55 262 141 628.5 0 141 263 239 628.5 0 239 264 293 628.5 0 293 265 223 628.5 0 223 266 67 628.5 0 67 267 54 628.5 0 54 268 49 628.5 0 49 269 74 628.5 0 74 270 77 628.5 0 77 271 130 628.5 0 130 272 125 628.5 0 125 273 89 628.5 0 89 274 56 628.5 0 56 275 73 628.5 0 73 276 78 628.5 0 78 277 71 628.5 0 71 278 80 628.5 0 80 279 53 628.5 0 53 280 63 628.5 0 63 281 90 628.5 0 90 282 89 628.5 0 89 283 158 628.5 0 158 284 142 628.5 0 142 285 163 628.5 0 163 286 109 628.5 0 109 287 71 628.5 0 71 288 61 628.5 0 61 289 75 628.5 0 75 290 113 628.5 0 113 291 198 628.5 0 198 292 207 628.5 0 207 293 71 628.5 0 71 294 90 628.5 0 90 295 53 628.5 0 53 296 83 628.5 0 83 297 94 628.5 0 94 298 107 628.5 0 107 299 86 628.5 0 86 300 74 628.5 0 74 301 96 628.5 0 96 Finished in 2.24 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 48,692 Reads with adapters: 17,408 (35.8%) Reads that were too short: 41 (0.1%) Reads written (passing filters): 17,367 (35.7%) Total basepairs processed: 12,684,450 bp Total written (filtered): 4,803,302 bp (37.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17408 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 19 0.0 3 0 0 10 9 16 216 0.0 3 0 177 23 16 17 16881 0.0 3 15232 1321 251 77 18 53 0.0 3 2 33 6 12 19 68 0.0 3 0 0 28 40 20 73 0.0 3 1 0 0 72 21 1 0.0 3 1 24 1 0.0 3 0 0 0 1 26 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 5 0.0 3 0 0 0 5 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 63 6 0.0 3 0 0 0 6 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 7 0.0 3 0 0 0 7 85 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 143 1 0.0 3 0 1 144 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 194 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 17,367 Reads with adapters: 49 (0.3%) Reads written (passing filters): 17,367 (100.0%) Total basepairs processed: 4,803,302 bp Total written (filtered): 4,803,145 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 49 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 38.8% C: 30.6% G: 26.5% T: 4.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 41 271.4 0 41 4 6 67.8 0 6 5 2 17.0 0 2