This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.98 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 51,796 (2.0%) Reads that were too short: 7,990 (0.3%) Reads written (passing filters): 43,806 (1.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 11,934,867 bp (1.5%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 51796 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 430 628.5 0 430 7 17492 628.5 0 17492 8 17552 628.5 0 17552 9 53 628.5 0 53 10 41 628.5 0 41 11 31 628.5 0 31 12 23 628.5 0 23 13 41 628.5 0 41 14 16 628.5 0 16 15 27 628.5 0 27 16 37 628.5 0 37 17 32 628.5 0 32 18 28 628.5 0 28 19 20 628.5 0 20 20 22 628.5 0 22 21 22 628.5 0 22 22 26 628.5 0 26 23 22 628.5 0 22 24 22 628.5 0 22 25 26 628.5 0 26 26 26 628.5 0 26 27 27 628.5 0 27 28 39 628.5 0 39 29 27 628.5 0 27 30 21 628.5 0 21 31 35 628.5 0 35 32 29 628.5 0 29 33 29 628.5 0 29 34 28 628.5 0 28 35 15 628.5 0 15 36 120 628.5 0 120 37 28 628.5 0 28 38 39 628.5 0 39 39 26 628.5 0 26 40 24 628.5 0 24 41 24 628.5 0 24 42 27 628.5 0 27 43 19 628.5 0 19 44 22 628.5 0 22 45 29 628.5 0 29 46 18 628.5 0 18 47 28 628.5 0 28 48 25 628.5 0 25 49 29 628.5 0 29 50 23 628.5 0 23 51 35 628.5 0 35 52 23 628.5 0 23 53 30 628.5 0 30 54 27 628.5 0 27 55 25 628.5 0 25 56 14 628.5 0 14 57 18 628.5 0 18 58 25 628.5 0 25 59 26 628.5 0 26 60 21 628.5 0 21 61 20 628.5 0 20 62 21 628.5 0 21 63 23 628.5 0 23 64 21 628.5 0 21 65 24 628.5 0 24 66 37 628.5 0 37 67 24 628.5 0 24 68 31 628.5 0 31 69 24 628.5 0 24 70 21 628.5 0 21 71 28 628.5 0 28 72 26 628.5 0 26 73 19 628.5 0 19 74 19 628.5 0 19 75 36 628.5 0 36 76 26 628.5 0 26 77 22 628.5 0 22 78 25 628.5 0 25 79 31 628.5 0 31 80 45 628.5 0 45 81 26 628.5 0 26 82 35 628.5 0 35 83 40 628.5 0 40 84 34 628.5 0 34 85 56 628.5 0 56 86 40 628.5 0 40 87 28 628.5 0 28 88 27 628.5 0 27 89 30 628.5 0 30 90 37 628.5 0 37 91 51 628.5 0 51 92 33 628.5 0 33 93 22 628.5 0 22 94 40 628.5 0 40 95 31 628.5 0 31 96 35 628.5 0 35 97 39 628.5 0 39 98 37 628.5 0 37 99 41 628.5 0 41 100 45 628.5 0 45 101 41 628.5 0 41 102 39 628.5 0 39 103 47 628.5 0 47 104 30 628.5 0 30 105 31 628.5 0 31 106 50 628.5 0 50 107 48 628.5 0 48 108 94 628.5 0 94 109 138 628.5 0 138 110 263 628.5 0 263 111 63 628.5 0 63 112 56 628.5 0 56 113 54 628.5 0 54 114 79 628.5 0 79 115 59 628.5 0 59 116 84 628.5 0 84 117 53 628.5 0 53 118 41 628.5 0 41 119 51 628.5 0 51 120 36 628.5 0 36 121 37 628.5 0 37 122 55 628.5 0 55 123 46 628.5 0 46 124 49 628.5 0 49 125 27 628.5 0 27 126 39 628.5 0 39 127 48 628.5 0 48 128 48 628.5 0 48 129 53 628.5 0 53 130 40 628.5 0 40 131 92 628.5 0 92 132 38 628.5 0 38 133 50 628.5 0 50 134 57 628.5 0 57 135 54 628.5 0 54 136 70 628.5 0 70 137 55 628.5 0 55 138 53 628.5 0 53 139 53 628.5 0 53 140 81 628.5 0 81 141 54 628.5 0 54 142 76 628.5 0 76 143 60 628.5 0 60 144 53 628.5 0 53 145 57 628.5 0 57 146 38 628.5 0 38 147 37 628.5 0 37 148 32 628.5 0 32 149 38 628.5 0 38 150 49 628.5 0 49 151 44 628.5 0 44 152 38 628.5 0 38 153 46 628.5 0 46 154 64 628.5 0 64 155 75 628.5 0 75 156 55 628.5 0 55 157 67 628.5 0 67 158 66 628.5 0 66 159 44 628.5 0 44 160 51 628.5 0 51 161 38 628.5 0 38 162 57 628.5 0 57 163 97 628.5 0 97 164 45 628.5 0 45 165 60 628.5 0 60 166 60 628.5 0 60 167 58 628.5 0 58 168 44 628.5 0 44 169 52 628.5 0 52 170 49 628.5 0 49 171 45 628.5 0 45 172 42 628.5 0 42 173 44 628.5 0 44 174 37 628.5 0 37 175 43 628.5 0 43 176 62 628.5 0 62 177 44 628.5 0 44 178 73 628.5 0 73 179 49 628.5 0 49 180 42 628.5 0 42 181 53 628.5 0 53 182 70 628.5 0 70 183 55 628.5 0 55 184 60 628.5 0 60 185 54 628.5 0 54 186 49 628.5 0 49 187 55 628.5 0 55 188 48 628.5 0 48 189 57 628.5 0 57 190 50 628.5 0 50 191 43 628.5 0 43 192 63 628.5 0 63 193 38 628.5 0 38 194 43 628.5 0 43 195 54 628.5 0 54 196 55 628.5 0 55 197 56 628.5 0 56 198 34 628.5 0 34 199 44 628.5 0 44 200 51 628.5 0 51 201 73 628.5 0 73 202 67 628.5 0 67 203 59 628.5 0 59 204 62 628.5 0 62 205 38 628.5 0 38 206 49 628.5 0 49 207 54 628.5 0 54 208 90 628.5 0 90 209 98 628.5 0 98 210 103 628.5 0 103 211 104 628.5 0 104 212 47 628.5 0 47 213 53 628.5 0 53 214 38 628.5 0 38 215 45 628.5 0 45 216 53 628.5 0 53 217 52 628.5 0 52 218 31 628.5 0 31 219 47 628.5 0 47 220 49 628.5 0 49 221 35 628.5 0 35 222 62 628.5 0 62 223 91 628.5 0 91 224 116 628.5 0 116 225 62 628.5 0 62 226 74 628.5 0 74 227 47 628.5 0 47 228 41 628.5 0 41 229 31 628.5 0 31 230 46 628.5 0 46 231 35 628.5 0 35 232 51 628.5 0 51 233 30 628.5 0 30 234 32 628.5 0 32 235 53 628.5 0 53 236 41 628.5 0 41 237 49 628.5 0 49 238 46 628.5 0 46 239 37 628.5 0 37 240 53 628.5 0 53 241 62 628.5 0 62 242 74 628.5 0 74 243 105 628.5 0 105 244 138 628.5 0 138 245 108 628.5 0 108 246 124 628.5 0 124 247 49 628.5 0 49 248 50 628.5 0 50 249 47 628.5 0 47 250 52 628.5 0 52 251 55 628.5 0 55 252 86 628.5 0 86 253 224 628.5 0 224 254 186 628.5 0 186 255 359 628.5 0 359 256 78 628.5 0 78 257 59 628.5 0 59 258 369 628.5 0 369 259 421 628.5 0 421 260 615 628.5 0 615 261 52 628.5 0 52 262 74 628.5 0 74 263 84 628.5 0 84 264 99 628.5 0 99 265 80 628.5 0 80 266 36 628.5 0 36 267 22 628.5 0 22 268 36 628.5 0 36 269 42 628.5 0 42 270 47 628.5 0 47 271 88 628.5 0 88 272 81 628.5 0 81 273 57 628.5 0 57 274 46 628.5 0 46 275 49 628.5 0 49 276 49 628.5 0 49 277 41 628.5 0 41 278 57 628.5 0 57 279 48 628.5 0 48 280 45 628.5 0 45 281 70 628.5 0 70 282 52 628.5 0 52 283 85 628.5 0 85 284 76 628.5 0 76 285 73 628.5 0 73 286 62 628.5 0 62 287 56 628.5 0 56 288 50 628.5 0 50 289 48 628.5 0 48 290 68 628.5 0 68 291 147 628.5 0 147 292 117 628.5 0 117 293 36 628.5 0 36 294 47 628.5 0 47 295 47 628.5 0 47 296 60 628.5 0 60 297 70 628.5 0 70 298 87 628.5 0 87 299 65 628.5 0 65 300 54 628.5 0 54 301 55 628.5 0 55 Finished in 2.25 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 43,806 Reads with adapters: 19,129 (43.7%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 19,125 (43.7%) Total basepairs processed: 11,934,867 bp Total written (filtered): 5,105,054 bp (42.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19129 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 2 0.0 3 0 0 2 19 150 0.0 3 0 147 3 20 17810 0.0 4 17539 260 11 21 37 0.0 4 7 29 1 22 8 0.0 4 4 1 2 0 1 23 14 0.0 4 0 0 0 13 1 24 1 0.0 4 0 0 0 1 26 6 0.0 4 0 0 0 0 6 29 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 15 0.0 4 0 0 0 0 15 127 1058 0.0 4 0 0 0 0 1058 128 15 0.0 4 0 0 0 0 15 130 1 0.0 4 0 0 0 0 1 143 1 0.0 4 1 211 1 0.0 4 0 1 234 1 0.0 4 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.67 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 19,125 Reads with adapters: 2,367 (12.4%) Reads written (passing filters): 19,125 (100.0%) Total basepairs processed: 5,105,054 bp Total written (filtered): 5,079,228 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2367 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 1.9% G: 95.1% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 298.8 0 12 4 4 74.7 0 4 9 9 0.1 0 1 8 10 267 0.0 1 16 251 11 2068 0.0 1 10 2058 12 4 0.0 1 1 3 14 1 0.0 1 0 1 62 1 0.0 1 1 151 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.