This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.37 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 141,687 (5.5%) Reads that were too short: 45,871 (1.8%) Reads written (passing filters): 95,816 (3.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 24,927,263 bp (3.2%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 141687 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4274 628.5 0 4274 7 26666 628.5 0 26666 8 1023 628.5 0 1023 9 33070 628.5 0 33070 10 105 628.5 0 105 11 78 628.5 0 78 12 74 628.5 0 74 13 97 628.5 0 97 14 80 628.5 0 80 15 68 628.5 0 68 16 52 628.5 0 52 17 137 628.5 0 137 18 229 628.5 0 229 19 170 628.5 0 170 20 54 628.5 0 54 21 76 628.5 0 76 22 80 628.5 0 80 23 90 628.5 0 90 24 57 628.5 0 57 25 71 628.5 0 71 26 67 628.5 0 67 27 70 628.5 0 70 28 58 628.5 0 58 29 58 628.5 0 58 30 80 628.5 0 80 31 89 628.5 0 89 32 100 628.5 0 100 33 87 628.5 0 87 34 78 628.5 0 78 35 50 628.5 0 50 36 68 628.5 0 68 37 82 628.5 0 82 38 83 628.5 0 83 39 82 628.5 0 82 40 98 628.5 0 98 41 100 628.5 0 100 42 109 628.5 0 109 43 92 628.5 0 92 44 82 628.5 0 82 45 103 628.5 0 103 46 99 628.5 0 99 47 407 628.5 0 407 48 547 628.5 0 547 49 888 628.5 0 888 50 142 628.5 0 142 51 76 628.5 0 76 52 83 628.5 0 83 53 81 628.5 0 81 54 85 628.5 0 85 55 84 628.5 0 84 56 72 628.5 0 72 57 74 628.5 0 74 58 112 628.5 0 112 59 207 628.5 0 207 60 296 628.5 0 296 61 250 628.5 0 250 62 96 628.5 0 96 63 82 628.5 0 82 64 93 628.5 0 93 65 73 628.5 0 73 66 76 628.5 0 76 67 69 628.5 0 69 68 81 628.5 0 81 69 81 628.5 0 81 70 131 628.5 0 131 71 137 628.5 0 137 72 173 628.5 0 173 73 68 628.5 0 68 74 73 628.5 0 73 75 77 628.5 0 77 76 105 628.5 0 105 77 302 628.5 0 302 78 269 628.5 0 269 79 281 628.5 0 281 80 90 628.5 0 90 81 115 628.5 0 115 82 281 628.5 0 281 83 140 628.5 0 140 84 620 628.5 0 620 85 566 628.5 0 566 86 673 628.5 0 673 87 73 628.5 0 73 88 62 628.5 0 62 89 74 628.5 0 74 90 52 628.5 0 52 91 95 628.5 0 95 92 57 628.5 0 57 93 90 628.5 0 90 94 60 628.5 0 60 95 45 628.5 0 45 96 83 628.5 0 83 97 99 628.5 0 99 98 47 628.5 0 47 99 76 628.5 0 76 100 91 628.5 0 91 101 53 628.5 0 53 102 43 628.5 0 43 103 52 628.5 0 52 104 57 628.5 0 57 105 72 628.5 0 72 106 73 628.5 0 73 107 88 628.5 0 88 108 67 628.5 0 67 109 90 628.5 0 90 110 76 628.5 0 76 111 75 628.5 0 75 112 375 628.5 0 375 113 386 628.5 0 386 114 406 628.5 0 406 115 89 628.5 0 89 116 80 628.5 0 80 117 155 628.5 0 155 118 163 628.5 0 163 119 220 628.5 0 220 120 97 628.5 0 97 121 67 628.5 0 67 122 79 628.5 0 79 123 65 628.5 0 65 124 70 628.5 0 70 125 81 628.5 0 81 126 104 628.5 0 104 127 127 628.5 0 127 128 199 628.5 0 199 129 256 628.5 0 256 130 134 628.5 0 134 131 184 628.5 0 184 132 367 628.5 0 367 133 561 628.5 0 561 134 718 628.5 0 718 135 330 628.5 0 330 136 228 628.5 0 228 137 127 628.5 0 127 138 146 628.5 0 146 139 189 628.5 0 189 140 146 628.5 0 146 141 440 628.5 0 440 142 2121 628.5 0 2121 143 2719 628.5 0 2719 144 80 628.5 0 80 145 67 628.5 0 67 146 88 628.5 0 88 147 99 628.5 0 99 148 114 628.5 0 114 149 103 628.5 0 103 150 93 628.5 0 93 151 126 628.5 0 126 152 343 628.5 0 343 153 565 628.5 0 565 154 523 628.5 0 523 155 215 628.5 0 215 156 58 628.5 0 58 157 71 628.5 0 71 158 71 628.5 0 71 159 87 628.5 0 87 160 58 628.5 0 58 161 54 628.5 0 54 162 67 628.5 0 67 163 70 628.5 0 70 164 94 628.5 0 94 165 49 628.5 0 49 166 80 628.5 0 80 167 57 628.5 0 57 168 65 628.5 0 65 169 92 628.5 0 92 170 78 628.5 0 78 171 88 628.5 0 88 172 75 628.5 0 75 173 85 628.5 0 85 174 77 628.5 0 77 175 65 628.5 0 65 176 62 628.5 0 62 177 62 628.5 0 62 178 58 628.5 0 58 179 74 628.5 0 74 180 61 628.5 0 61 181 66 628.5 0 66 182 70 628.5 0 70 183 67 628.5 0 67 184 90 628.5 0 90 185 90 628.5 0 90 186 90 628.5 0 90 187 117 628.5 0 117 188 93 628.5 0 93 189 110 628.5 0 110 190 125 628.5 0 125 191 129 628.5 0 129 192 89 628.5 0 89 193 102 628.5 0 102 194 76 628.5 0 76 195 81 628.5 0 81 196 95 628.5 0 95 197 112 628.5 0 112 198 87 628.5 0 87 199 109 628.5 0 109 200 120 628.5 0 120 201 103 628.5 0 103 202 90 628.5 0 90 203 117 628.5 0 117 204 93 628.5 0 93 205 92 628.5 0 92 206 118 628.5 0 118 207 106 628.5 0 106 208 82 628.5 0 82 209 87 628.5 0 87 210 101 628.5 0 101 211 120 628.5 0 120 212 190 628.5 0 190 213 185 628.5 0 185 214 266 628.5 0 266 215 188 628.5 0 188 216 171 628.5 0 171 217 190 628.5 0 190 218 304 628.5 0 304 219 341 628.5 0 341 220 225 628.5 0 225 221 103 628.5 0 103 222 88 628.5 0 88 223 57 628.5 0 57 224 92 628.5 0 92 225 145 628.5 0 145 226 139 628.5 0 139 227 133 628.5 0 133 228 123 628.5 0 123 229 122 628.5 0 122 230 231 628.5 0 231 231 177 628.5 0 177 232 203 628.5 0 203 233 163 628.5 0 163 234 230 628.5 0 230 235 868 628.5 0 868 236 1150 628.5 0 1150 237 1335 628.5 0 1335 238 582 628.5 0 582 239 734 628.5 0 734 240 534 628.5 0 534 241 199 628.5 0 199 242 109 628.5 0 109 243 168 628.5 0 168 244 197 628.5 0 197 245 362 628.5 0 362 246 468 628.5 0 468 247 558 628.5 0 558 248 317 628.5 0 317 249 194 628.5 0 194 250 219 628.5 0 219 251 252 628.5 0 252 252 512 628.5 0 512 253 350 628.5 0 350 254 525 628.5 0 525 255 660 628.5 0 660 256 1383 628.5 0 1383 257 1146 628.5 0 1146 258 850 628.5 0 850 259 143 628.5 0 143 260 171 628.5 0 171 261 183 628.5 0 183 262 112 628.5 0 112 263 137 628.5 0 137 264 122 628.5 0 122 265 96 628.5 0 96 266 119 628.5 0 119 267 101 628.5 0 101 268 103 628.5 0 103 269 104 628.5 0 104 270 113 628.5 0 113 271 114 628.5 0 114 272 141 628.5 0 141 273 294 628.5 0 294 274 332 628.5 0 332 275 192 628.5 0 192 276 120 628.5 0 120 277 125 628.5 0 125 278 160 628.5 0 160 279 196 628.5 0 196 280 199 628.5 0 199 281 144 628.5 0 144 282 231 628.5 0 231 283 425 628.5 0 425 284 883 628.5 0 883 285 1149 628.5 0 1149 286 557 628.5 0 557 287 579 628.5 0 579 288 384 628.5 0 384 289 264 628.5 0 264 290 199 628.5 0 199 291 297 628.5 0 297 292 258 628.5 0 258 293 324 628.5 0 324 294 306 628.5 0 306 295 420 628.5 0 420 296 508 628.5 0 508 297 598 628.5 0 598 298 5238 628.5 0 5238 299 6642 628.5 0 6642 300 3427 628.5 0 3427 301 917 628.5 0 917 Finished in 4.36 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 95,816 Reads with adapters: 36,339 (37.9%) Reads that were too short: 224 (0.2%) Reads written (passing filters): 36,115 (37.7%) Total basepairs processed: 24,927,263 bp Total written (filtered): 9,846,181 bp (39.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36339 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 24 0.0 3 0 0 20 4 16 305 0.0 3 0 241 45 19 17 33772 0.0 3 30565 2550 517 140 18 109 0.0 3 13 66 12 18 19 1198 0.0 3 1002 84 65 47 20 111 0.0 3 0 3 1 107 21 6 0.0 3 1 0 4 1 22 2 0.0 3 0 0 0 2 23 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 2 0.0 3 0 0 0 2 32 2 0.0 3 1 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 3 0.0 3 0 0 0 3 39 98 0.0 3 0 0 2 96 40 4 0.0 3 0 0 0 4 41 3 0.0 3 0 0 0 3 42 22 0.0 3 0 0 1 21 44 2 0.0 3 0 0 0 2 45 4 0.0 3 0 0 1 3 47 10 0.0 3 0 0 0 10 48 9 0.0 3 0 0 1 8 49 1 0.0 3 0 0 1 50 131 0.0 3 0 0 1 130 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 15 0.0 3 0 0 0 15 54 3 0.0 3 0 0 1 2 56 2 0.0 3 0 0 0 2 59 20 0.0 3 0 0 0 20 62 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 65 5 0.0 3 0 0 0 5 66 4 0.0 3 0 0 0 4 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 0 4 75 3 0.0 3 0 0 0 3 76 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 32 0.0 3 0 0 0 32 80 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 20 0.0 3 0 0 2 18 88 2 0.0 3 0 0 0 2 89 7 0.0 3 0 0 0 7 90 5 0.0 3 0 0 0 5 91 6 0.0 3 0 0 0 6 92 4 0.0 3 0 0 0 4 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 1 95 5 0.0 3 0 0 1 4 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 99 11 0.0 3 0 0 1 10 100 4 0.0 3 0 0 0 4 101 53 0.0 3 0 0 1 52 102 4 0.0 3 0 0 0 4 103 4 0.0 3 0 0 0 4 104 5 0.0 3 0 0 0 5 105 1 0.0 3 0 0 0 1 106 4 0.0 3 0 0 0 4 107 1 0.0 3 0 0 0 1 108 10 0.0 3 0 0 0 10 109 2 0.0 3 0 0 0 2 110 4 0.0 3 0 0 0 4 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 6 0.0 3 0 0 0 6 114 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 1 1 119 10 0.0 3 0 0 1 9 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 1 123 8 0.0 3 0 0 0 8 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 132 9 0.0 3 0 0 0 9 133 4 0.0 3 0 0 0 4 134 1 0.0 3 0 0 0 1 135 7 0.0 3 0 0 1 6 138 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 0 4 140 2 0.0 3 0 0 0 2 141 3 0.0 3 0 0 0 3 142 6 0.0 3 0 0 1 5 143 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 145 4 0.0 3 0 0 0 4 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 149 4 0.0 3 0 0 0 4 150 1 0.0 3 0 0 0 1 151 9 0.0 3 0 0 0 9 152 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 5 0.0 3 0 0 0 5 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 1 158 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 4 0.0 3 0 0 1 3 164 2 0.0 3 0 0 0 2 165 2 0.0 3 0 0 0 2 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 169 4 0.0 3 0 0 0 4 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 1 2 175 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 2 0.0 3 0 0 0 2 183 4 0.0 3 0 0 0 4 186 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 1 2 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 193 3 0.0 3 0 0 1 2 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 4 0.0 3 0 0 0 4 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 1 212 1 0.0 3 0 0 0 1 215 2 0.0 3 0 0 1 1 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 1 1 218 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 246 2 0.0 3 0 0 0 2 248 1 0.0 3 0 0 0 1 252 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 5 0.0 3 0 0 0 5 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 5 0.0 3 0 0 0 5 282 5 0.0 3 0 0 0 5 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 294 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.27 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 36,115 Reads with adapters: 1,320 (3.7%) Reads written (passing filters): 36,115 (100.0%) Total basepairs processed: 9,846,181 bp Total written (filtered): 9,842,151 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1320 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 53.9% C: 12.3% G: 31.2% T: 2.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1259 564.3 0 1259 4 60 141.1 0 60 13 1 0.0 1 0 1