This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.96 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 115,729 (4.5%) Reads that were too short: 19,531 (0.8%) Reads written (passing filters): 96,198 (3.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 25,473,254 bp (3.3%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 115729 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4012 628.5 0 4012 7 33605 628.5 0 33605 8 854 628.5 0 854 9 31546 628.5 0 31546 10 120 628.5 0 120 11 103 628.5 0 103 12 92 628.5 0 92 13 110 628.5 0 110 14 97 628.5 0 97 15 92 628.5 0 92 16 63 628.5 0 63 17 90 628.5 0 90 18 118 628.5 0 118 19 93 628.5 0 93 20 85 628.5 0 85 21 108 628.5 0 108 22 76 628.5 0 76 23 91 628.5 0 91 24 91 628.5 0 91 25 79 628.5 0 79 26 81 628.5 0 81 27 98 628.5 0 98 28 78 628.5 0 78 29 74 628.5 0 74 30 73 628.5 0 73 31 55 628.5 0 55 32 80 628.5 0 80 33 81 628.5 0 81 34 85 628.5 0 85 35 84 628.5 0 84 36 64 628.5 0 64 37 86 628.5 0 86 38 79 628.5 0 79 39 103 628.5 0 103 40 108 628.5 0 108 41 92 628.5 0 92 42 123 628.5 0 123 43 81 628.5 0 81 44 82 628.5 0 82 45 89 628.5 0 89 46 84 628.5 0 84 47 141 628.5 0 141 48 179 628.5 0 179 49 164 628.5 0 164 50 99 628.5 0 99 51 74 628.5 0 74 52 153 628.5 0 153 53 99 628.5 0 99 54 104 628.5 0 104 55 135 628.5 0 135 56 106 628.5 0 106 57 85 628.5 0 85 58 101 628.5 0 101 59 146 628.5 0 146 60 182 628.5 0 182 61 200 628.5 0 200 62 109 628.5 0 109 63 91 628.5 0 91 64 79 628.5 0 79 65 95 628.5 0 95 66 85 628.5 0 85 67 103 628.5 0 103 68 92 628.5 0 92 69 108 628.5 0 108 70 133 628.5 0 133 71 113 628.5 0 113 72 173 628.5 0 173 73 105 628.5 0 105 74 78 628.5 0 78 75 92 628.5 0 92 76 121 628.5 0 121 77 135 628.5 0 135 78 108 628.5 0 108 79 129 628.5 0 129 80 112 628.5 0 112 81 101 628.5 0 101 82 122 628.5 0 122 83 112 628.5 0 112 84 380 628.5 0 380 85 305 628.5 0 305 86 417 628.5 0 417 87 76 628.5 0 76 88 71 628.5 0 71 89 122 628.5 0 122 90 54 628.5 0 54 91 94 628.5 0 94 92 94 628.5 0 94 93 84 628.5 0 84 94 117 628.5 0 117 95 78 628.5 0 78 96 76 628.5 0 76 97 97 628.5 0 97 98 53 628.5 0 53 99 78 628.5 0 78 100 82 628.5 0 82 101 93 628.5 0 93 102 102 628.5 0 102 103 53 628.5 0 53 104 58 628.5 0 58 105 82 628.5 0 82 106 115 628.5 0 115 107 115 628.5 0 115 108 93 628.5 0 93 109 113 628.5 0 113 110 82 628.5 0 82 111 89 628.5 0 89 112 434 628.5 0 434 113 430 628.5 0 430 114 480 628.5 0 480 115 70 628.5 0 70 116 70 628.5 0 70 117 106 628.5 0 106 118 91 628.5 0 91 119 110 628.5 0 110 120 71 628.5 0 71 121 72 628.5 0 72 122 81 628.5 0 81 123 91 628.5 0 91 124 81 628.5 0 81 125 78 628.5 0 78 126 92 628.5 0 92 127 124 628.5 0 124 128 153 628.5 0 153 129 123 628.5 0 123 130 126 628.5 0 126 131 136 628.5 0 136 132 186 628.5 0 186 133 261 628.5 0 261 134 331 628.5 0 331 135 190 628.5 0 190 136 130 628.5 0 130 137 83 628.5 0 83 138 97 628.5 0 97 139 124 628.5 0 124 140 150 628.5 0 150 141 414 628.5 0 414 142 1986 628.5 0 1986 143 2611 628.5 0 2611 144 91 628.5 0 91 145 78 628.5 0 78 146 96 628.5 0 96 147 100 628.5 0 100 148 100 628.5 0 100 149 99 628.5 0 99 150 75 628.5 0 75 151 100 628.5 0 100 152 209 628.5 0 209 153 247 628.5 0 247 154 235 628.5 0 235 155 133 628.5 0 133 156 105 628.5 0 105 157 92 628.5 0 92 158 81 628.5 0 81 159 108 628.5 0 108 160 87 628.5 0 87 161 87 628.5 0 87 162 85 628.5 0 85 163 94 628.5 0 94 164 85 628.5 0 85 165 80 628.5 0 80 166 115 628.5 0 115 167 90 628.5 0 90 168 63 628.5 0 63 169 105 628.5 0 105 170 113 628.5 0 113 171 86 628.5 0 86 172 97 628.5 0 97 173 95 628.5 0 95 174 79 628.5 0 79 175 88 628.5 0 88 176 80 628.5 0 80 177 76 628.5 0 76 178 87 628.5 0 87 179 73 628.5 0 73 180 81 628.5 0 81 181 67 628.5 0 67 182 91 628.5 0 91 183 71 628.5 0 71 184 110 628.5 0 110 185 121 628.5 0 121 186 81 628.5 0 81 187 154 628.5 0 154 188 90 628.5 0 90 189 93 628.5 0 93 190 85 628.5 0 85 191 94 628.5 0 94 192 93 628.5 0 93 193 69 628.5 0 69 194 69 628.5 0 69 195 80 628.5 0 80 196 95 628.5 0 95 197 77 628.5 0 77 198 81 628.5 0 81 199 90 628.5 0 90 200 75 628.5 0 75 201 93 628.5 0 93 202 102 628.5 0 102 203 149 628.5 0 149 204 80 628.5 0 80 205 88 628.5 0 88 206 63 628.5 0 63 207 77 628.5 0 77 208 56 628.5 0 56 209 79 628.5 0 79 210 75 628.5 0 75 211 81 628.5 0 81 212 83 628.5 0 83 213 119 628.5 0 119 214 93 628.5 0 93 215 71 628.5 0 71 216 115 628.5 0 115 217 82 628.5 0 82 218 90 628.5 0 90 219 83 628.5 0 83 220 77 628.5 0 77 221 57 628.5 0 57 222 61 628.5 0 61 223 48 628.5 0 48 224 63 628.5 0 63 225 93 628.5 0 93 226 103 628.5 0 103 227 89 628.5 0 89 228 82 628.5 0 82 229 61 628.5 0 61 230 135 628.5 0 135 231 96 628.5 0 96 232 93 628.5 0 93 233 74 628.5 0 74 234 90 628.5 0 90 235 254 628.5 0 254 236 304 628.5 0 304 237 342 628.5 0 342 238 129 628.5 0 129 239 135 628.5 0 135 240 98 628.5 0 98 241 77 628.5 0 77 242 65 628.5 0 65 243 67 628.5 0 67 244 75 628.5 0 75 245 114 628.5 0 114 246 130 628.5 0 130 247 117 628.5 0 117 248 136 628.5 0 136 249 78 628.5 0 78 250 78 628.5 0 78 251 88 628.5 0 88 252 124 628.5 0 124 253 122 628.5 0 122 254 123 628.5 0 123 255 172 628.5 0 172 256 394 628.5 0 394 257 389 628.5 0 389 258 288 628.5 0 288 259 65 628.5 0 65 260 110 628.5 0 110 261 113 628.5 0 113 262 60 628.5 0 60 263 64 628.5 0 64 264 69 628.5 0 69 265 58 628.5 0 58 266 76 628.5 0 76 267 72 628.5 0 72 268 64 628.5 0 64 269 74 628.5 0 74 270 73 628.5 0 73 271 59 628.5 0 59 272 80 628.5 0 80 273 157 628.5 0 157 274 326 628.5 0 326 275 219 628.5 0 219 276 77 628.5 0 77 277 77 628.5 0 77 278 70 628.5 0 70 279 81 628.5 0 81 280 91 628.5 0 91 281 89 628.5 0 89 282 92 628.5 0 92 283 127 628.5 0 127 284 198 628.5 0 198 285 250 628.5 0 250 286 162 628.5 0 162 287 221 628.5 0 221 288 198 628.5 0 198 289 104 628.5 0 104 290 110 628.5 0 110 291 167 628.5 0 167 292 164 628.5 0 164 293 215 628.5 0 215 294 136 628.5 0 136 295 225 628.5 0 225 296 182 628.5 0 182 297 230 628.5 0 230 298 2395 628.5 0 2395 299 3092 628.5 0 3092 300 2017 628.5 0 2017 301 315 628.5 0 315 Finished in 4.86 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 96,198 Reads with adapters: 35,824 (37.2%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 35,808 (37.2%) Total basepairs processed: 25,473,254 bp Total written (filtered): 9,773,315 bp (38.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35824 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 20 0.0 3 0 0 20 19 418 0.0 3 0 409 6 2 1 20 33451 0.0 4 32622 791 32 3 3 21 105 0.0 4 26 78 1 22 1428 0.0 4 1390 25 12 0 1 23 43 0.0 4 3 1 0 28 11 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 1 26 1 0.0 4 1 40 1 0.0 4 1 46 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 1 119 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 306 0.0 4 0 0 0 0 306 128 2 0.0 4 0 0 1 0 1 129 19 0.0 4 0 0 0 0 19 130 2 0.0 4 0 0 0 0 2 136 1 0.0 4 1 145 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 1 188 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.22 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 35,808 Reads with adapters: 1,296 (3.6%) Reads written (passing filters): 35,808 (100.0%) Total basepairs processed: 9,773,315 bp Total written (filtered): 9,758,265 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1296 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.2% C: 5.1% G: 89.5% T: 1.1% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 68 559.5 0 68 4 6 139.9 0 6 5 4 35.0 0 4 6 3 8.7 0 3 7 1 2.2 0 1 9 82 0.1 0 4 78 10 109 0.0 1 2 107 11 890 0.0 1 8 882 12 113 0.0 1 4 109 13 11 0.0 1 1 10 111 1 0.0 1 1 152 3 0.0 1 0 3 169 1 0.0 1 1 171 1 0.0 1 0 1 210 1 0.0 1 1 269 1 0.0 1 1 274 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.