This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.09 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 136,499 (5.3%) Reads that were too short: 51,751 (2.0%) Reads written (passing filters): 84,748 (3.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 22,752,560 bp (2.9%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 136499 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 933 628.5 0 933 7 36293 628.5 0 36293 8 729 628.5 0 729 9 29974 628.5 0 29974 10 481 628.5 0 481 11 32 628.5 0 32 12 48 628.5 0 48 13 43 628.5 0 43 14 20 628.5 0 20 15 21 628.5 0 21 16 30 628.5 0 30 17 16 628.5 0 16 18 14 628.5 0 14 19 15 628.5 0 15 20 28 628.5 0 28 21 49 628.5 0 49 22 20 628.5 0 20 23 30 628.5 0 30 24 18 628.5 0 18 25 27 628.5 0 27 26 33 628.5 0 33 27 11 628.5 0 11 28 29 628.5 0 29 29 35 628.5 0 35 30 30 628.5 0 30 31 16 628.5 0 16 32 29 628.5 0 29 33 32 628.5 0 32 34 25 628.5 0 25 35 18 628.5 0 18 36 15 628.5 0 15 37 29 628.5 0 29 38 23 628.5 0 23 39 25 628.5 0 25 40 37 628.5 0 37 41 28 628.5 0 28 42 29 628.5 0 29 43 27 628.5 0 27 44 22 628.5 0 22 45 22 628.5 0 22 46 23 628.5 0 23 47 27 628.5 0 27 48 21 628.5 0 21 49 34 628.5 0 34 50 18 628.5 0 18 51 17 628.5 0 17 52 26 628.5 0 26 53 40 628.5 0 40 54 39 628.5 0 39 55 104 628.5 0 104 56 71 628.5 0 71 57 56 628.5 0 56 58 18 628.5 0 18 59 60 628.5 0 60 60 42 628.5 0 42 61 76 628.5 0 76 62 34 628.5 0 34 63 37 628.5 0 37 64 42 628.5 0 42 65 47 628.5 0 47 66 17 628.5 0 17 67 35 628.5 0 35 68 27 628.5 0 27 69 54 628.5 0 54 70 183 628.5 0 183 71 76 628.5 0 76 72 46 628.5 0 46 73 37 628.5 0 37 74 104 628.5 0 104 75 103 628.5 0 103 76 157 628.5 0 157 77 44 628.5 0 44 78 45 628.5 0 45 79 186 628.5 0 186 80 63 628.5 0 63 81 254 628.5 0 254 82 375 628.5 0 375 83 305 628.5 0 305 84 573 628.5 0 573 85 119 628.5 0 119 86 122 628.5 0 122 87 31 628.5 0 31 88 37 628.5 0 37 89 66 628.5 0 66 90 36 628.5 0 36 91 20 628.5 0 20 92 33 628.5 0 33 93 26 628.5 0 26 94 13 628.5 0 13 95 33 628.5 0 33 96 38 628.5 0 38 97 32 628.5 0 32 98 27 628.5 0 27 99 29 628.5 0 29 100 23 628.5 0 23 101 18 628.5 0 18 102 32 628.5 0 32 103 40 628.5 0 40 104 65 628.5 0 65 105 29 628.5 0 29 106 35 628.5 0 35 107 31 628.5 0 31 108 29 628.5 0 29 109 19 628.5 0 19 110 41 628.5 0 41 111 41 628.5 0 41 112 45 628.5 0 45 113 68 628.5 0 68 114 55 628.5 0 55 115 41 628.5 0 41 116 22 628.5 0 22 117 35 628.5 0 35 118 45 628.5 0 45 119 39 628.5 0 39 120 44 628.5 0 44 121 158 628.5 0 158 122 277 628.5 0 277 123 372 628.5 0 372 124 49 628.5 0 49 125 112 628.5 0 112 126 73 628.5 0 73 127 88 628.5 0 88 128 70 628.5 0 70 129 60 628.5 0 60 130 40 628.5 0 40 131 34 628.5 0 34 132 33 628.5 0 33 133 68 628.5 0 68 134 49 628.5 0 49 135 43 628.5 0 43 136 21 628.5 0 21 137 23 628.5 0 23 138 64 628.5 0 64 139 50 628.5 0 50 140 54 628.5 0 54 141 34 628.5 0 34 142 20 628.5 0 20 143 36 628.5 0 36 144 33 628.5 0 33 145 40 628.5 0 40 146 27 628.5 0 27 147 52 628.5 0 52 148 51 628.5 0 51 149 25 628.5 0 25 150 35 628.5 0 35 151 43 628.5 0 43 152 65 628.5 0 65 153 93 628.5 0 93 154 62 628.5 0 62 155 35 628.5 0 35 156 35 628.5 0 35 157 51 628.5 0 51 158 44 628.5 0 44 159 41 628.5 0 41 160 37 628.5 0 37 161 38 628.5 0 38 162 48 628.5 0 48 163 76 628.5 0 76 164 47 628.5 0 47 165 75 628.5 0 75 166 91 628.5 0 91 167 699 628.5 0 699 168 370 628.5 0 370 169 785 628.5 0 785 170 132 628.5 0 132 171 281 628.5 0 281 172 190 628.5 0 190 173 353 628.5 0 353 174 82 628.5 0 82 175 49 628.5 0 49 176 43 628.5 0 43 177 40 628.5 0 40 178 44 628.5 0 44 179 40 628.5 0 40 180 184 628.5 0 184 181 198 628.5 0 198 182 363 628.5 0 363 183 98 628.5 0 98 184 100 628.5 0 100 185 96 628.5 0 96 186 98 628.5 0 98 187 85 628.5 0 85 188 109 628.5 0 109 189 94 628.5 0 94 190 85 628.5 0 85 191 93 628.5 0 93 192 59 628.5 0 59 193 71 628.5 0 71 194 88 628.5 0 88 195 71 628.5 0 71 196 84 628.5 0 84 197 94 628.5 0 94 198 131 628.5 0 131 199 215 628.5 0 215 200 1280 628.5 0 1280 201 661 628.5 0 661 202 607 628.5 0 607 203 118 628.5 0 118 204 65 628.5 0 65 205 120 628.5 0 120 206 157 628.5 0 157 207 129 628.5 0 129 208 124 628.5 0 124 209 51 628.5 0 51 210 58 628.5 0 58 211 126 628.5 0 126 212 63 628.5 0 63 213 94 628.5 0 94 214 166 628.5 0 166 215 181 628.5 0 181 216 217 628.5 0 217 217 132 628.5 0 132 218 130 628.5 0 130 219 174 628.5 0 174 220 176 628.5 0 176 221 144 628.5 0 144 222 119 628.5 0 119 223 189 628.5 0 189 224 263 628.5 0 263 225 338 628.5 0 338 226 694 628.5 0 694 227 1233 628.5 0 1233 228 12491 628.5 0 12491 229 8739 628.5 0 8739 230 8472 628.5 0 8472 231 250 628.5 0 250 232 124 628.5 0 124 233 62 628.5 0 62 234 52 628.5 0 52 235 45 628.5 0 45 236 87 628.5 0 87 237 83 628.5 0 83 238 101 628.5 0 101 239 119 628.5 0 119 240 99 628.5 0 99 241 78 628.5 0 78 242 80 628.5 0 80 243 122 628.5 0 122 244 104 628.5 0 104 245 135 628.5 0 135 246 221 628.5 0 221 247 296 628.5 0 296 248 388 628.5 0 388 249 335 628.5 0 335 250 200 628.5 0 200 251 93 628.5 0 93 252 78 628.5 0 78 253 71 628.5 0 71 254 103 628.5 0 103 255 112 628.5 0 112 256 348 628.5 0 348 257 411 628.5 0 411 258 408 628.5 0 408 259 123 628.5 0 123 260 139 628.5 0 139 261 384 628.5 0 384 262 641 628.5 0 641 263 998 628.5 0 998 264 280 628.5 0 280 265 365 628.5 0 365 266 148 628.5 0 148 267 226 628.5 0 226 268 787 628.5 0 787 269 765 628.5 0 765 270 616 628.5 0 616 271 208 628.5 0 208 272 110 628.5 0 110 273 86 628.5 0 86 274 126 628.5 0 126 275 152 628.5 0 152 276 138 628.5 0 138 277 149 628.5 0 149 278 187 628.5 0 187 279 95 628.5 0 95 280 122 628.5 0 122 281 206 628.5 0 206 282 281 628.5 0 281 283 345 628.5 0 345 284 128 628.5 0 128 285 78 628.5 0 78 286 107 628.5 0 107 287 159 628.5 0 159 288 232 628.5 0 232 289 136 628.5 0 136 290 89 628.5 0 89 291 89 628.5 0 89 292 351 628.5 0 351 293 436 628.5 0 436 294 399 628.5 0 399 295 143 628.5 0 143 296 99 628.5 0 99 297 86 628.5 0 86 298 100 628.5 0 100 299 131 628.5 0 131 300 417 628.5 0 417 301 719 628.5 0 719 Finished in 3.91 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 84,748 Reads with adapters: 36,619 (43.2%) Reads that were too short: 142 (0.2%) Reads written (passing filters): 36,477 (43.0%) Total basepairs processed: 22,752,560 bp Total written (filtered): 10,090,422 bp (44.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36619 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 26 0.0 3 0 0 13 13 16 325 0.0 3 0 261 44 20 17 35675 0.0 3 31747 3160 622 146 18 80 0.0 3 9 61 5 5 19 149 0.0 3 2 2 62 83 20 123 0.0 3 1 1 0 121 21 1 0.0 3 0 0 1 24 6 0.0 3 1 0 0 5 27 3 0.0 3 0 0 0 3 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 0 0 0 1 39 4 0.0 3 0 0 0 4 40 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 3 0.0 3 0 0 0 3 46 15 0.0 3 0 0 0 15 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 70 2 0.0 3 0 0 0 2 71 12 0.0 3 0 0 0 12 72 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 88 5 0.0 3 0 0 0 5 89 1 0.0 3 0 0 0 1 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 6 0.0 3 0 0 2 4 99 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 110 1 0.0 3 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 2 0.0 3 0 0 0 2 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 5 0.0 3 0 0 0 5 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 156 2 0.0 3 0 0 0 2 159 1 0.0 3 0 0 1 161 4 0.0 3 0 0 0 4 162 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 182 3 0.0 3 0 0 0 3 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 3 0.0 3 0 0 0 3 195 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 228 2 0.0 3 0 0 1 1 230 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 264 4 0.0 3 0 0 0 4 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 282 1 0.0 3 0 0 0 1 283 3 0.0 3 0 0 1 2 287 1 0.0 3 0 0 0 1 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 36,477 Reads with adapters: 113 (0.3%) Reads written (passing filters): 36,477 (100.0%) Total basepairs processed: 10,090,422 bp Total written (filtered): 10,090,079 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 113 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.6% C: 48.7% G: 16.8% T: 23.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 109 570.0 0 109 4 4 142.5 0 4