This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGACCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.02 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 144,357 (5.6%) Reads that were too short: 59,485 (2.3%) Reads written (passing filters): 84,872 (3.3%) Total basepairs processed: 774,927,510 bp Total written (filtered): 23,198,699 bp (3.0%) === Adapter 1 === Sequence: AGACCG; Type: regular 5'; Length: 6; Trimmed: 144357 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 463 628.5 0 463 7 32665 628.5 0 32665 8 538 628.5 0 538 9 38459 628.5 0 38459 10 67 628.5 0 67 11 15 628.5 0 15 12 27 628.5 0 27 13 5 628.5 0 5 14 13 628.5 0 13 15 16 628.5 0 16 16 14 628.5 0 14 17 14 628.5 0 14 18 15 628.5 0 15 19 14 628.5 0 14 20 7 628.5 0 7 21 15 628.5 0 15 22 16 628.5 0 16 23 9 628.5 0 9 24 7 628.5 0 7 25 11 628.5 0 11 26 24 628.5 0 24 27 14 628.5 0 14 28 17 628.5 0 17 29 13 628.5 0 13 30 22 628.5 0 22 31 12 628.5 0 12 32 17 628.5 0 17 33 28 628.5 0 28 34 23 628.5 0 23 35 15 628.5 0 15 36 24 628.5 0 24 37 33 628.5 0 33 38 12 628.5 0 12 39 17 628.5 0 17 40 19 628.5 0 19 41 16 628.5 0 16 42 14 628.5 0 14 43 14 628.5 0 14 44 20 628.5 0 20 45 10 628.5 0 10 46 17 628.5 0 17 47 19 628.5 0 19 48 16 628.5 0 16 49 18 628.5 0 18 50 12 628.5 0 12 51 9 628.5 0 9 52 14 628.5 0 14 53 12 628.5 0 12 54 11 628.5 0 11 55 22 628.5 0 22 56 23 628.5 0 23 57 18 628.5 0 18 58 12 628.5 0 12 59 20 628.5 0 20 60 30 628.5 0 30 61 30 628.5 0 30 62 22 628.5 0 22 63 23 628.5 0 23 64 17 628.5 0 17 65 22 628.5 0 22 66 18 628.5 0 18 67 33 628.5 0 33 68 20 628.5 0 20 69 33 628.5 0 33 70 149 628.5 0 149 71 51 628.5 0 51 72 34 628.5 0 34 73 28 628.5 0 28 74 86 628.5 0 86 75 121 628.5 0 121 76 125 628.5 0 125 77 37 628.5 0 37 78 42 628.5 0 42 79 232 628.5 0 232 80 63 628.5 0 63 81 150 628.5 0 150 82 355 628.5 0 355 83 154 628.5 0 154 84 529 628.5 0 529 85 31 628.5 0 31 86 40 628.5 0 40 87 24 628.5 0 24 88 25 628.5 0 25 89 20 628.5 0 20 90 18 628.5 0 18 91 28 628.5 0 28 92 31 628.5 0 31 93 29 628.5 0 29 94 26 628.5 0 26 95 31 628.5 0 31 96 28 628.5 0 28 97 27 628.5 0 27 98 26 628.5 0 26 99 32 628.5 0 32 100 22 628.5 0 22 101 34 628.5 0 34 102 31 628.5 0 31 103 30 628.5 0 30 104 27 628.5 0 27 105 37 628.5 0 37 106 31 628.5 0 31 107 27 628.5 0 27 108 25 628.5 0 25 109 21 628.5 0 21 110 29 628.5 0 29 111 38 628.5 0 38 112 32 628.5 0 32 113 38 628.5 0 38 114 38 628.5 0 38 115 36 628.5 0 36 116 25 628.5 0 25 117 27 628.5 0 27 118 30 628.5 0 30 119 39 628.5 0 39 120 26 628.5 0 26 121 44 628.5 0 44 122 65 628.5 0 65 123 143 628.5 0 143 124 49 628.5 0 49 125 99 628.5 0 99 126 47 628.5 0 47 127 37 628.5 0 37 128 51 628.5 0 51 129 39 628.5 0 39 130 35 628.5 0 35 131 32 628.5 0 32 132 32 628.5 0 32 133 38 628.5 0 38 134 40 628.5 0 40 135 30 628.5 0 30 136 42 628.5 0 42 137 37 628.5 0 37 138 64 628.5 0 64 139 45 628.5 0 45 140 46 628.5 0 46 141 32 628.5 0 32 142 26 628.5 0 26 143 38 628.5 0 38 144 41 628.5 0 41 145 51 628.5 0 51 146 54 628.5 0 54 147 48 628.5 0 48 148 60 628.5 0 60 149 54 628.5 0 54 150 65 628.5 0 65 151 52 628.5 0 52 152 56 628.5 0 56 153 44 628.5 0 44 154 55 628.5 0 55 155 55 628.5 0 55 156 60 628.5 0 60 157 69 628.5 0 69 158 57 628.5 0 57 159 50 628.5 0 50 160 67 628.5 0 67 161 61 628.5 0 61 162 74 628.5 0 74 163 82 628.5 0 82 164 72 628.5 0 72 165 92 628.5 0 92 166 118 628.5 0 118 167 731 628.5 0 731 168 242 628.5 0 242 169 687 628.5 0 687 170 87 628.5 0 87 171 200 628.5 0 200 172 128 628.5 0 128 173 199 628.5 0 199 174 79 628.5 0 79 175 76 628.5 0 76 176 89 628.5 0 89 177 69 628.5 0 69 178 81 628.5 0 81 179 100 628.5 0 100 180 115 628.5 0 115 181 112 628.5 0 112 182 172 628.5 0 172 183 100 628.5 0 100 184 98 628.5 0 98 185 100 628.5 0 100 186 127 628.5 0 127 187 100 628.5 0 100 188 99 628.5 0 99 189 131 628.5 0 131 190 89 628.5 0 89 191 135 628.5 0 135 192 100 628.5 0 100 193 96 628.5 0 96 194 104 628.5 0 104 195 110 628.5 0 110 196 109 628.5 0 109 197 100 628.5 0 100 198 124 628.5 0 124 199 137 628.5 0 137 200 496 628.5 0 496 201 362 628.5 0 362 202 276 628.5 0 276 203 138 628.5 0 138 204 102 628.5 0 102 205 127 628.5 0 127 206 112 628.5 0 112 207 133 628.5 0 133 208 115 628.5 0 115 209 97 628.5 0 97 210 124 628.5 0 124 211 210 628.5 0 210 212 116 628.5 0 116 213 152 628.5 0 152 214 143 628.5 0 143 215 162 628.5 0 162 216 200 628.5 0 200 217 165 628.5 0 165 218 149 628.5 0 149 219 144 628.5 0 144 220 150 628.5 0 150 221 178 628.5 0 178 222 188 628.5 0 188 223 253 628.5 0 253 224 318 628.5 0 318 225 380 628.5 0 380 226 773 628.5 0 773 227 1467 628.5 0 1467 228 16340 628.5 0 16340 229 11304 628.5 0 11304 230 10598 628.5 0 10598 231 216 628.5 0 216 232 114 628.5 0 114 233 122 628.5 0 122 234 117 628.5 0 117 235 112 628.5 0 112 236 127 628.5 0 127 237 131 628.5 0 131 238 131 628.5 0 131 239 131 628.5 0 131 240 132 628.5 0 132 241 129 628.5 0 129 242 131 628.5 0 131 243 128 628.5 0 128 244 112 628.5 0 112 245 129 628.5 0 129 246 158 628.5 0 158 247 174 628.5 0 174 248 175 628.5 0 175 249 181 628.5 0 181 250 146 628.5 0 146 251 136 628.5 0 136 252 127 628.5 0 127 253 117 628.5 0 117 254 175 628.5 0 175 255 155 628.5 0 155 256 308 628.5 0 308 257 315 628.5 0 315 258 288 628.5 0 288 259 168 628.5 0 168 260 134 628.5 0 134 261 288 628.5 0 288 262 441 628.5 0 441 263 655 628.5 0 655 264 266 628.5 0 266 265 290 628.5 0 290 266 160 628.5 0 160 267 200 628.5 0 200 268 826 628.5 0 826 269 777 628.5 0 777 270 633 628.5 0 633 271 218 628.5 0 218 272 188 628.5 0 188 273 138 628.5 0 138 274 152 628.5 0 152 275 138 628.5 0 138 276 204 628.5 0 204 277 268 628.5 0 268 278 299 628.5 0 299 279 150 628.5 0 150 280 178 628.5 0 178 281 160 628.5 0 160 282 206 628.5 0 206 283 296 628.5 0 296 284 139 628.5 0 139 285 133 628.5 0 133 286 133 628.5 0 133 287 171 628.5 0 171 288 221 628.5 0 221 289 160 628.5 0 160 290 150 628.5 0 150 291 145 628.5 0 145 292 164 628.5 0 164 293 194 628.5 0 194 294 186 628.5 0 186 295 121 628.5 0 121 296 161 628.5 0 161 297 138 628.5 0 138 298 136 628.5 0 136 299 157 628.5 0 157 300 239 628.5 0 239 301 273 628.5 0 273 Finished in 4.33 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 84,872 Reads with adapters: 40,603 (47.8%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 40,595 (47.8%) Total basepairs processed: 23,198,699 bp Total written (filtered): 10,927,895 bp (47.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 40603 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 12 0.0 3 0 0 11 1 19 306 0.0 3 0 303 3 20 39054 0.0 4 38464 564 20 4 2 21 83 0.0 4 25 57 1 22 7 0.0 4 1 0 6 23 30 0.0 4 0 1 2 25 2 24 2 0.0 4 1 0 0 0 1 68 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 124 3 0.0 4 0 0 0 0 3 125 6 0.0 4 0 0 0 0 6 126 14 0.0 4 0 0 0 0 14 127 1076 0.0 4 0 0 0 0 1076 179 1 0.0 4 1 212 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.45 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 40,595 Reads with adapters: 7,056 (17.4%) Reads written (passing filters): 40,595 (100.0%) Total basepairs processed: 10,927,895 bp Total written (filtered): 10,850,747 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7056 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.9% G: 95.1% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 634.3 0 8 4 46 158.6 0 46 5 1 39.6 0 1 6 1 9.9 0 1 7 1 2.5 0 1 8 1 0.6 0 1 9 3921 0.2 0 121 3800 10 2566 0.0 1 83 2483 11 456 0.0 1 4 452 12 9 0.0 1 1 8 13 2 0.0 1 0 2 150 2 0.0 1 0 2 208 3 0.0 1 1 2 223 2 0.0 1 2 224 1 0.0 1 1 256 33 0.0 1 32 1 257 2 0.0 1 2 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.