This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 71.90 s (28 us/read; 2.15 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 1,132,152 (44.0%) Reads that were too short: 34,095 (1.3%) Reads written (passing filters): 1,098,057 (42.7%) Total basepairs processed: 774,927,510 bp Total written (filtered): 299,518,735 bp (38.7%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1132152 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2920 628.5 0 2920 7 80859 628.5 0 80859 8 5959 628.5 0 5959 9 81046 628.5 0 81046 10 613 628.5 0 613 11 469 628.5 0 469 12 7164 628.5 0 7164 13 2148 628.5 0 2148 14 419 628.5 0 419 15 1047 628.5 0 1047 16 456 628.5 0 456 17 643 628.5 0 643 18 1593 628.5 0 1593 19 778 628.5 0 778 20 834 628.5 0 834 21 332 628.5 0 332 22 597 628.5 0 597 23 1958 628.5 0 1958 24 2175 628.5 0 2175 25 4045 628.5 0 4045 26 5737 628.5 0 5737 27 6104 628.5 0 6104 28 15237 628.5 0 15237 29 23677 628.5 0 23677 30 304141 628.5 0 304141 31 172286 628.5 0 172286 32 356403 628.5 0 356403 33 1447 628.5 0 1447 34 147 628.5 0 147 35 348 628.5 0 348 36 554 628.5 0 554 37 528 628.5 0 528 38 36 628.5 0 36 39 32 628.5 0 32 40 28 628.5 0 28 41 63 628.5 0 63 42 36 628.5 0 36 43 43 628.5 0 43 44 112 628.5 0 112 45 33 628.5 0 33 46 43 628.5 0 43 47 49 628.5 0 49 48 31 628.5 0 31 49 37 628.5 0 37 50 35 628.5 0 35 51 40 628.5 0 40 52 38 628.5 0 38 53 54 628.5 0 54 54 404 628.5 0 404 55 658 628.5 0 658 56 760 628.5 0 760 57 42 628.5 0 42 58 32 628.5 0 32 59 15 628.5 0 15 60 23 628.5 0 23 61 25 628.5 0 25 62 27 628.5 0 27 63 16 628.5 0 16 64 27 628.5 0 27 65 28 628.5 0 28 66 24 628.5 0 24 67 17 628.5 0 17 68 40 628.5 0 40 69 71 628.5 0 71 70 110 628.5 0 110 71 66 628.5 0 66 72 30 628.5 0 30 73 33 628.5 0 33 74 26 628.5 0 26 75 29 628.5 0 29 76 24 628.5 0 24 77 27 628.5 0 27 78 19 628.5 0 19 79 14 628.5 0 14 80 28 628.5 0 28 81 35 628.5 0 35 82 99 628.5 0 99 83 34 628.5 0 34 84 31 628.5 0 31 85 29 628.5 0 29 86 32 628.5 0 32 87 52 628.5 0 52 88 48 628.5 0 48 89 123 628.5 0 123 90 48 628.5 0 48 91 38 628.5 0 38 92 24 628.5 0 24 93 15 628.5 0 15 94 22 628.5 0 22 95 33 628.5 0 33 96 44 628.5 0 44 97 31 628.5 0 31 98 31 628.5 0 31 99 32 628.5 0 32 100 39 628.5 0 39 101 39 628.5 0 39 102 184 628.5 0 184 103 317 628.5 0 317 104 1016 628.5 0 1016 105 758 628.5 0 758 106 443 628.5 0 443 107 72 628.5 0 72 108 26 628.5 0 26 109 29 628.5 0 29 110 21 628.5 0 21 111 44 628.5 0 44 112 50 628.5 0 50 113 62 628.5 0 62 114 44 628.5 0 44 115 24 628.5 0 24 116 28 628.5 0 28 117 23 628.5 0 23 118 40 628.5 0 40 119 38 628.5 0 38 120 32 628.5 0 32 121 23 628.5 0 23 122 32 628.5 0 32 123 64 628.5 0 64 124 185 628.5 0 185 125 193 628.5 0 193 126 211 628.5 0 211 127 50 628.5 0 50 128 35 628.5 0 35 129 26 628.5 0 26 130 25 628.5 0 25 131 33 628.5 0 33 132 44 628.5 0 44 133 64 628.5 0 64 134 56 628.5 0 56 135 50 628.5 0 50 136 55 628.5 0 55 137 430 628.5 0 430 138 1024 628.5 0 1024 139 1787 628.5 0 1787 140 83 628.5 0 83 141 202 628.5 0 202 142 185 628.5 0 185 143 230 628.5 0 230 144 49 628.5 0 49 145 26 628.5 0 26 146 43 628.5 0 43 147 67 628.5 0 67 148 71 628.5 0 71 149 191 628.5 0 191 150 59 628.5 0 59 151 98 628.5 0 98 152 34 628.5 0 34 153 25 628.5 0 25 154 39 628.5 0 39 155 33 628.5 0 33 156 34 628.5 0 34 157 24 628.5 0 24 158 43 628.5 0 43 159 84 628.5 0 84 160 37 628.5 0 37 161 85 628.5 0 85 162 38 628.5 0 38 163 61 628.5 0 61 164 52 628.5 0 52 165 28 628.5 0 28 166 42 628.5 0 42 167 65 628.5 0 65 168 72 628.5 0 72 169 33 628.5 0 33 170 40 628.5 0 40 171 54 628.5 0 54 172 24 628.5 0 24 173 27 628.5 0 27 174 30 628.5 0 30 175 23 628.5 0 23 176 26 628.5 0 26 177 46 628.5 0 46 178 36 628.5 0 36 179 35 628.5 0 35 180 34 628.5 0 34 181 48 628.5 0 48 182 90 628.5 0 90 183 90 628.5 0 90 184 108 628.5 0 108 185 75 628.5 0 75 186 71 628.5 0 71 187 36 628.5 0 36 188 48 628.5 0 48 189 41 628.5 0 41 190 30 628.5 0 30 191 26 628.5 0 26 192 28 628.5 0 28 193 35 628.5 0 35 194 32 628.5 0 32 195 37 628.5 0 37 196 34 628.5 0 34 197 26 628.5 0 26 198 39 628.5 0 39 199 54 628.5 0 54 200 43 628.5 0 43 201 52 628.5 0 52 202 32 628.5 0 32 203 29 628.5 0 29 204 82 628.5 0 82 205 178 628.5 0 178 206 315 628.5 0 315 207 209 628.5 0 209 208 39 628.5 0 39 209 42 628.5 0 42 210 47 628.5 0 47 211 55 628.5 0 55 212 90 628.5 0 90 213 94 628.5 0 94 214 52 628.5 0 52 215 56 628.5 0 56 216 55 628.5 0 55 217 32 628.5 0 32 218 46 628.5 0 46 219 68 628.5 0 68 220 72 628.5 0 72 221 53 628.5 0 53 222 37 628.5 0 37 223 24 628.5 0 24 224 50 628.5 0 50 225 56 628.5 0 56 226 114 628.5 0 114 227 125 628.5 0 125 228 131 628.5 0 131 229 49 628.5 0 49 230 49 628.5 0 49 231 112 628.5 0 112 232 165 628.5 0 165 233 129 628.5 0 129 234 59 628.5 0 59 235 31 628.5 0 31 236 51 628.5 0 51 237 45 628.5 0 45 238 38 628.5 0 38 239 49 628.5 0 49 240 40 628.5 0 40 241 57 628.5 0 57 242 46 628.5 0 46 243 50 628.5 0 50 244 46 628.5 0 46 245 72 628.5 0 72 246 91 628.5 0 91 247 141 628.5 0 141 248 76 628.5 0 76 249 81 628.5 0 81 250 124 628.5 0 124 251 70 628.5 0 70 252 74 628.5 0 74 253 115 628.5 0 115 254 653 628.5 0 653 255 1168 628.5 0 1168 256 816 628.5 0 816 257 599 628.5 0 599 258 442 628.5 0 442 259 431 628.5 0 431 260 5052 628.5 0 5052 261 8621 628.5 0 8621 262 4472 628.5 0 4472 263 92 628.5 0 92 264 56 628.5 0 56 265 65 628.5 0 65 266 66 628.5 0 66 267 119 628.5 0 119 268 148 628.5 0 148 269 164 628.5 0 164 270 104 628.5 0 104 271 54 628.5 0 54 272 241 628.5 0 241 273 270 628.5 0 270 274 329 628.5 0 329 275 838 628.5 0 838 276 764 628.5 0 764 277 872 628.5 0 872 278 511 628.5 0 511 279 417 628.5 0 417 280 339 628.5 0 339 281 450 628.5 0 450 282 530 628.5 0 530 283 564 628.5 0 564 284 47 628.5 0 47 285 29 628.5 0 29 286 35 628.5 0 35 287 53 628.5 0 53 288 37 628.5 0 37 289 59 628.5 0 59 290 55 628.5 0 55 291 41 628.5 0 41 292 44 628.5 0 44 293 51 628.5 0 51 294 80 628.5 0 80 295 63 628.5 0 63 296 60 628.5 0 60 297 15 628.5 0 15 298 32 628.5 0 32 299 26 628.5 0 26 300 40 628.5 0 40 301 38 628.5 0 38 Finished in 46.10 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 1,098,057 Reads with adapters: 89,981 (8.2%) Reads that were too short: 1,017 (0.1%) Reads written (passing filters): 88,964 (8.1%) Total basepairs processed: 299,518,735 bp Total written (filtered): 24,384,282 bp (8.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 89981 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 34 0.0 2 0 0 0 34 15 68 0.0 3 0 0 48 20 16 1053 0.0 3 0 859 123 71 17 86040 0.0 3 77952 6346 1369 373 18 267 0.0 3 10 200 25 32 19 313 0.0 3 17 7 160 129 20 575 0.0 3 342 35 9 189 21 5 0.0 3 0 3 1 1 22 2 0.0 3 1 0 0 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 33 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 3 0.0 3 0 0 0 3 44 1 0.0 3 0 0 0 1 45 7 0.0 3 0 0 0 7 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 8 0.0 3 0 0 0 8 49 2 0.0 3 0 0 0 2 51 6 0.0 3 0 0 0 6 53 1 0.0 3 0 0 0 1 54 5 0.0 3 0 0 0 5 55 12 0.0 3 0 0 0 12 56 1 0.0 3 0 1 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 1 65 2 0.0 3 0 0 0 2 67 20 0.0 3 0 1 2 17 68 14 0.0 3 0 0 0 14 69 8 0.0 3 0 0 0 8 70 5 0.0 3 0 0 0 5 71 5 0.0 3 0 0 0 5 72 4 0.0 3 0 0 0 4 73 2 0.0 3 0 1 0 1 75 3 0.0 3 1 0 0 2 76 17 0.0 3 0 0 1 16 77 1 0.0 3 1 78 1 0.0 3 0 0 0 1 80 2 0.0 3 0 1 0 1 81 2 0.0 3 0 0 1 1 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 3 0.0 3 0 0 0 3 88 3 0.0 3 1 0 0 2 89 2 0.0 3 1 0 0 1 90 2 0.0 3 0 0 0 2 92 2 0.0 3 0 1 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 9 0.0 3 0 0 0 9 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 1 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 7 0.0 3 0 0 0 7 106 3 0.0 3 1 0 0 2 108 2 0.0 3 0 0 0 2 112 4 0.0 3 0 1 0 3 113 6 0.0 3 0 0 0 6 115 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 15 0.0 3 0 0 0 15 118 2 0.0 3 0 0 0 2 119 2 0.0 3 0 0 0 2 120 5 0.0 3 0 0 0 5 121 3 0.0 3 0 0 0 3 125 3 0.0 3 0 0 0 3 127 12 0.0 3 0 0 0 12 128 3 0.0 3 0 0 0 3 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 15 0.0 3 0 0 1 14 134 2 0.0 3 0 0 0 2 136 6 0.0 3 0 0 1 5 137 12 0.0 3 0 0 0 12 138 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 140 2 0.0 3 0 0 0 2 141 23 0.0 3 0 0 0 23 142 2 0.0 3 0 0 1 1 143 3 0.0 3 0 0 0 3 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 155 4 0.0 3 0 0 0 4 156 16 0.0 3 0 0 0 16 157 7 0.0 3 0 0 0 7 159 6 0.0 3 0 0 0 6 160 9 0.0 3 0 0 0 9 161 21 0.0 3 0 0 0 21 162 7 0.0 3 0 0 0 7 163 2 0.0 3 0 0 0 2 164 13 0.0 3 0 0 1 12 165 2 0.0 3 0 0 1 1 167 10 0.0 3 0 0 0 10 168 6 0.0 3 0 0 0 6 169 41 0.0 3 0 0 0 41 170 10 0.0 3 1 0 1 8 171 152 0.0 3 0 0 8 144 172 23 0.0 3 0 0 0 23 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 0 3 177 1 0.0 3 0 0 0 1 179 4 0.0 3 0 0 0 4 180 7 0.0 3 0 0 1 6 181 50 0.0 3 0 0 1 49 182 55 0.0 3 0 0 1 54 183 10 0.0 3 0 0 1 9 184 8 0.0 3 0 0 0 8 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 188 2 0.0 3 0 0 0 2 190 5 0.0 3 0 0 0 5 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 0 1 193 6 0.0 3 0 0 0 6 194 16 0.0 3 0 0 1 15 195 22 0.0 3 0 0 0 22 197 1 0.0 3 0 0 0 1 198 41 0.0 3 0 0 0 41 199 13 0.0 3 0 0 0 13 200 3 0.0 3 0 0 1 2 201 3 0.0 3 0 0 0 3 202 2 0.0 3 0 0 0 2 203 3 0.0 3 0 0 0 3 204 3 0.0 3 0 0 0 3 205 2 0.0 3 0 0 0 2 207 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 212 3 0.0 3 0 0 0 3 213 4 0.0 3 0 0 0 4 214 8 0.0 3 0 0 0 8 215 16 0.0 3 0 0 1 15 216 6 0.0 3 0 0 0 6 217 19 0.0 3 0 0 0 19 218 4 0.0 3 0 0 0 4 219 2 0.0 3 0 0 0 2 220 4 0.0 3 0 0 0 4 221 6 0.0 3 0 0 1 5 224 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 232 2 0.0 3 0 0 0 2 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 8 0.0 3 0 0 0 8 239 3 0.0 3 0 0 0 3 240 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 244 1 0.0 3 0 0 0 1 245 2 0.0 3 0 0 0 2 246 1 0.0 3 0 0 0 1 247 27 0.0 3 0 0 0 27 248 9 0.0 3 0 0 0 9 249 5 0.0 3 0 0 2 3 250 4 0.0 3 0 0 0 4 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 255 6 0.0 3 0 0 0 6 256 65 0.0 3 0 0 0 65 257 71 0.0 3 0 0 1 70 258 146 0.0 3 0 0 5 141 259 76 0.0 3 0 0 3 73 260 19 0.0 3 0 0 0 19 261 4 0.0 3 0 0 0 4 262 8 0.0 3 0 0 0 8 263 1 0.0 3 0 0 0 1 264 8 0.0 3 0 0 0 8 265 14 0.0 3 0 0 1 13 266 4 0.0 3 0 0 0 4 267 3 0.0 3 0 0 0 3 268 4 0.0 3 0 0 0 4 269 5 0.0 3 0 0 0 5 270 9 0.0 3 0 0 0 9 271 13 0.0 3 0 0 0 13 272 4 0.0 3 0 0 0 4 273 6 0.0 3 0 0 0 6 274 3 0.0 3 0 0 0 3 276 3 0.0 3 0 0 0 3 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 6 0.0 3 0 0 0 6 280 5 0.0 3 0 0 0 5 281 11 0.0 3 0 0 0 11 282 11 0.0 3 0 0 0 11 283 7 0.0 3 0 0 2 5 285 2 0.0 3 0 0 0 2 287 2 0.0 3 0 0 0 2 288 3 0.0 3 0 0 0 3 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 292 2 0.0 3 0 0 1 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.11 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 88,964 Reads with adapters: 1,797 (2.0%) Reads written (passing filters): 88,964 (100.0%) Total basepairs processed: 24,384,282 bp Total written (filtered): 24,378,640 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1797 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 45.2% C: 9.7% G: 43.5% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1555 1390.1 0 1555 4 237 347.5 0 237 5 4 86.9 0 4 9 1 0.3 0 0 1