This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 72.85 s (28 us/read; 2.12 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 1,330,368 (51.7%) Reads that were too short: 56,785 (2.2%) Reads written (passing filters): 1,273,583 (49.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 347,782,834 bp (44.9%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1330368 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1882 628.5 0 1882 7 98677 628.5 0 98677 8 3957 628.5 0 3957 9 78287 628.5 0 78287 10 558 628.5 0 558 11 436 628.5 0 436 12 7042 628.5 0 7042 13 2708 628.5 0 2708 14 477 628.5 0 477 15 1206 628.5 0 1206 16 599 628.5 0 599 17 776 628.5 0 776 18 1922 628.5 0 1922 19 1016 628.5 0 1016 20 1010 628.5 0 1010 21 406 628.5 0 406 22 696 628.5 0 696 23 2111 628.5 0 2111 24 2119 628.5 0 2119 25 4032 628.5 0 4032 26 6153 628.5 0 6153 27 6679 628.5 0 6679 28 17608 628.5 0 17608 29 24486 628.5 0 24486 30 361835 628.5 0 361835 31 207122 628.5 0 207122 32 428886 628.5 0 428886 33 1548 628.5 0 1548 34 156 628.5 0 156 35 197 628.5 0 197 36 197 628.5 0 197 37 196 628.5 0 196 38 26 628.5 0 26 39 31 628.5 0 31 40 32 628.5 0 32 41 46 628.5 0 46 42 29 628.5 0 29 43 37 628.5 0 37 44 93 628.5 0 93 45 35 628.5 0 35 46 31 628.5 0 31 47 36 628.5 0 36 48 15 628.5 0 15 49 25 628.5 0 25 50 34 628.5 0 34 51 29 628.5 0 29 52 29 628.5 0 29 53 19 628.5 0 19 54 142 628.5 0 142 55 135 628.5 0 135 56 145 628.5 0 145 57 32 628.5 0 32 58 25 628.5 0 25 59 20 628.5 0 20 60 32 628.5 0 32 61 24 628.5 0 24 62 23 628.5 0 23 63 21 628.5 0 21 64 25 628.5 0 25 65 31 628.5 0 31 66 27 628.5 0 27 67 29 628.5 0 29 68 39 628.5 0 39 69 34 628.5 0 34 70 54 628.5 0 54 71 43 628.5 0 43 72 22 628.5 0 22 73 27 628.5 0 27 74 28 628.5 0 28 75 27 628.5 0 27 76 27 628.5 0 27 77 25 628.5 0 25 78 23 628.5 0 23 79 19 628.5 0 19 80 31 628.5 0 31 81 36 628.5 0 36 82 69 628.5 0 69 83 51 628.5 0 51 84 41 628.5 0 41 85 33 628.5 0 33 86 65 628.5 0 65 87 57 628.5 0 57 88 45 628.5 0 45 89 150 628.5 0 150 90 51 628.5 0 51 91 35 628.5 0 35 92 41 628.5 0 41 93 21 628.5 0 21 94 23 628.5 0 23 95 32 628.5 0 32 96 30 628.5 0 30 97 39 628.5 0 39 98 36 628.5 0 36 99 38 628.5 0 38 100 41 628.5 0 41 101 30 628.5 0 30 102 80 628.5 0 80 103 122 628.5 0 122 104 346 628.5 0 346 105 329 628.5 0 329 106 216 628.5 0 216 107 67 628.5 0 67 108 19 628.5 0 19 109 42 628.5 0 42 110 39 628.5 0 39 111 52 628.5 0 52 112 32 628.5 0 32 113 41 628.5 0 41 114 48 628.5 0 48 115 35 628.5 0 35 116 40 628.5 0 40 117 33 628.5 0 33 118 42 628.5 0 42 119 38 628.5 0 38 120 31 628.5 0 31 121 27 628.5 0 27 122 31 628.5 0 31 123 40 628.5 0 40 124 129 628.5 0 129 125 159 628.5 0 159 126 236 628.5 0 236 127 37 628.5 0 37 128 38 628.5 0 38 129 36 628.5 0 36 130 29 628.5 0 29 131 24 628.5 0 24 132 25 628.5 0 25 133 35 628.5 0 35 134 34 628.5 0 34 135 38 628.5 0 38 136 43 628.5 0 43 137 92 628.5 0 92 138 187 628.5 0 187 139 371 628.5 0 371 140 47 628.5 0 47 141 94 628.5 0 94 142 82 628.5 0 82 143 98 628.5 0 98 144 32 628.5 0 32 145 36 628.5 0 36 146 34 628.5 0 34 147 26 628.5 0 26 148 39 628.5 0 39 149 112 628.5 0 112 150 28 628.5 0 28 151 67 628.5 0 67 152 33 628.5 0 33 153 29 628.5 0 29 154 28 628.5 0 28 155 46 628.5 0 46 156 24 628.5 0 24 157 32 628.5 0 32 158 44 628.5 0 44 159 74 628.5 0 74 160 34 628.5 0 34 161 70 628.5 0 70 162 40 628.5 0 40 163 32 628.5 0 32 164 37 628.5 0 37 165 26 628.5 0 26 166 38 628.5 0 38 167 52 628.5 0 52 168 37 628.5 0 37 169 34 628.5 0 34 170 40 628.5 0 40 171 55 628.5 0 55 172 35 628.5 0 35 173 22 628.5 0 22 174 36 628.5 0 36 175 30 628.5 0 30 176 40 628.5 0 40 177 53 628.5 0 53 178 40 628.5 0 40 179 25 628.5 0 25 180 28 628.5 0 28 181 42 628.5 0 42 182 57 628.5 0 57 183 61 628.5 0 61 184 66 628.5 0 66 185 35 628.5 0 35 186 31 628.5 0 31 187 39 628.5 0 39 188 40 628.5 0 40 189 38 628.5 0 38 190 37 628.5 0 37 191 39 628.5 0 39 192 49 628.5 0 49 193 43 628.5 0 43 194 43 628.5 0 43 195 50 628.5 0 50 196 34 628.5 0 34 197 25 628.5 0 25 198 43 628.5 0 43 199 37 628.5 0 37 200 44 628.5 0 44 201 57 628.5 0 57 202 39 628.5 0 39 203 27 628.5 0 27 204 41 628.5 0 41 205 70 628.5 0 70 206 148 628.5 0 148 207 77 628.5 0 77 208 33 628.5 0 33 209 41 628.5 0 41 210 37 628.5 0 37 211 37 628.5 0 37 212 43 628.5 0 43 213 48 628.5 0 48 214 36 628.5 0 36 215 34 628.5 0 34 216 47 628.5 0 47 217 48 628.5 0 48 218 53 628.5 0 53 219 53 628.5 0 53 220 61 628.5 0 61 221 53 628.5 0 53 222 43 628.5 0 43 223 41 628.5 0 41 224 29 628.5 0 29 225 43 628.5 0 43 226 79 628.5 0 79 227 80 628.5 0 80 228 80 628.5 0 80 229 53 628.5 0 53 230 41 628.5 0 41 231 83 628.5 0 83 232 83 628.5 0 83 233 68 628.5 0 68 234 55 628.5 0 55 235 47 628.5 0 47 236 52 628.5 0 52 237 64 628.5 0 64 238 38 628.5 0 38 239 67 628.5 0 67 240 58 628.5 0 58 241 52 628.5 0 52 242 64 628.5 0 64 243 66 628.5 0 66 244 65 628.5 0 65 245 86 628.5 0 86 246 106 628.5 0 106 247 200 628.5 0 200 248 94 628.5 0 94 249 114 628.5 0 114 250 182 628.5 0 182 251 87 628.5 0 87 252 101 628.5 0 101 253 169 628.5 0 169 254 814 628.5 0 814 255 1513 628.5 0 1513 256 840 628.5 0 840 257 711 628.5 0 711 258 388 628.5 0 388 259 474 628.5 0 474 260 9090 628.5 0 9090 261 17463 628.5 0 17463 262 8998 628.5 0 8998 263 138 628.5 0 138 264 74 628.5 0 74 265 69 628.5 0 69 266 66 628.5 0 66 267 104 628.5 0 104 268 112 628.5 0 112 269 111 628.5 0 111 270 96 628.5 0 96 271 81 628.5 0 81 272 466 628.5 0 466 273 552 628.5 0 552 274 709 628.5 0 709 275 1570 628.5 0 1570 276 1856 628.5 0 1856 277 1870 628.5 0 1870 278 1481 628.5 0 1481 279 1105 628.5 0 1105 280 451 628.5 0 451 281 434 628.5 0 434 282 488 628.5 0 488 283 457 628.5 0 457 284 32 628.5 0 32 285 32 628.5 0 32 286 41 628.5 0 41 287 38 628.5 0 38 288 35 628.5 0 35 289 47 628.5 0 47 290 38 628.5 0 38 291 32 628.5 0 32 292 41 628.5 0 41 293 42 628.5 0 42 294 54 628.5 0 54 295 55 628.5 0 55 296 42 628.5 0 42 297 23 628.5 0 23 298 26 628.5 0 26 299 42 628.5 0 42 300 27 628.5 0 27 301 41 628.5 0 41 Finished in 58.60 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 1,273,583 Reads with adapters: 100,555 (7.9%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 100,507 (7.9%) Total basepairs processed: 347,782,834 bp Total written (filtered): 27,372,345 bp (7.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 100555 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 20 0.0 3 0 0 20 19 738 0.0 3 0 720 15 2 1 20 97974 0.0 4 96496 1429 42 6 1 21 211 0.0 4 26 184 1 22 15 0.0 4 0 1 14 23 42 0.0 4 1 0 3 33 5 24 2 0.0 4 0 0 0 1 1 37 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 63 1 0.0 4 1 70 1 0.0 4 1 75 1 0.0 4 1 77 1 0.0 4 1 78 2 0.0 4 2 79 1 0.0 4 1 80 3 0.0 4 0 0 0 0 3 81 1 0.0 4 0 1 84 1 0.0 4 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 1 111 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 116 2 0.0 4 2 123 2 0.0 4 0 0 0 0 2 124 297 0.0 4 0 0 0 0 297 125 4 0.0 4 0 0 0 0 4 126 7 0.0 4 0 0 0 0 7 127 1066 0.0 4 0 0 0 0 1066 128 1 0.0 4 0 0 0 0 1 129 3 0.0 4 1 0 0 0 2 130 14 0.0 4 0 0 0 1 13 135 1 0.0 4 1 142 10 0.0 4 0 0 0 0 10 148 50 0.0 4 0 0 0 0 50 149 23 0.0 4 0 1 0 0 22 150 1 0.0 4 0 0 0 0 1 153 2 0.0 4 1 0 0 0 1 154 4 0.0 4 0 1 1 0 2 166 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 1 173 1 0.0 4 1 176 1 0.0 4 1 181 1 0.0 4 0 0 0 0 1 186 1 0.0 4 1 189 1 0.0 4 1 190 1 0.0 4 1 191 1 0.0 4 1 201 3 0.0 4 0 1 0 0 2 205 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 1 215 1 0.0 4 1 217 1 0.0 4 1 220 1 0.0 4 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 1 0.0 4 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 1 237 1 0.0 4 0 0 0 0 1 247 2 0.0 4 0 0 0 0 2 248 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 1 260 1 0.0 4 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 269 1 0.0 4 1 273 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.42 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 100,507 Reads with adapters: 2,270 (2.3%) Reads written (passing filters): 100,507 (100.0%) Total basepairs processed: 27,372,345 bp Total written (filtered): 27,271,910 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2270 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.2% C: 17.4% G: 72.1% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 203 1570.4 0 203 4 20 392.6 0 20 5 8 98.2 0 8 6 1 24.5 0 1 9 9 0.4 0 2 7 10 17 0.1 1 1 16 11 1413 0.0 1 21 1392 12 291 0.0 1 3 288 13 1 0.0 1 0 1 15 3 0.0 1 0 3 49 1 0.0 1 1 140 1 0.0 1 1 149 1 0.0 1 1 152 5 0.0 1 0 5 191 1 0.0 1 1 210 5 0.0 1 2 3 269 283 0.0 1 265 18 270 7 0.0 1 7