This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 68.89 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 119,589 (4.6%) Reads that were too short: 29,017 (1.1%) Reads written (passing filters): 90,572 (3.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 21,939,142 bp (2.8%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 119589 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1417 628.5 0 1417 7 566 628.5 0 566 8 21992 628.5 0 21992 9 20385 628.5 0 20385 10 76 628.5 0 76 11 42 628.5 0 42 12 57 628.5 0 57 13 50 628.5 0 50 14 52 628.5 0 52 15 49 628.5 0 49 16 43 628.5 0 43 17 51 628.5 0 51 18 64 628.5 0 64 19 49 628.5 0 49 20 34 628.5 0 34 21 39 628.5 0 39 22 49 628.5 0 49 23 51 628.5 0 51 24 84 628.5 0 84 25 83 628.5 0 83 26 39 628.5 0 39 27 41 628.5 0 41 28 30 628.5 0 30 29 32 628.5 0 32 30 52 628.5 0 52 31 45 628.5 0 45 32 57 628.5 0 57 33 49 628.5 0 49 34 47 628.5 0 47 35 91 628.5 0 91 36 37 628.5 0 37 37 39 628.5 0 39 38 39 628.5 0 39 39 41 628.5 0 41 40 42 628.5 0 42 41 55 628.5 0 55 42 71 628.5 0 71 43 53 628.5 0 53 44 41 628.5 0 41 45 26 628.5 0 26 46 69 628.5 0 69 47 50 628.5 0 50 48 33 628.5 0 33 49 46 628.5 0 46 50 39 628.5 0 39 51 79 628.5 0 79 52 118 628.5 0 118 53 88 628.5 0 88 54 469 628.5 0 469 55 80 628.5 0 80 56 52 628.5 0 52 57 102 628.5 0 102 58 71 628.5 0 71 59 57 628.5 0 57 60 59 628.5 0 59 61 67 628.5 0 67 62 103 628.5 0 103 63 111 628.5 0 111 64 108 628.5 0 108 65 182 628.5 0 182 66 163 628.5 0 163 67 425 628.5 0 425 68 595 628.5 0 595 69 6547 628.5 0 6547 70 2694 628.5 0 2694 71 7161 628.5 0 7161 72 69 628.5 0 69 73 56 628.5 0 56 74 44 628.5 0 44 75 74 628.5 0 74 76 66 628.5 0 66 77 55 628.5 0 55 78 58 628.5 0 58 79 56 628.5 0 56 80 222 628.5 0 222 81 118 628.5 0 118 82 187 628.5 0 187 83 63 628.5 0 63 84 62 628.5 0 62 85 49 628.5 0 49 86 67 628.5 0 67 87 117 628.5 0 117 88 83 628.5 0 83 89 74 628.5 0 74 90 78 628.5 0 78 91 70 628.5 0 70 92 60 628.5 0 60 93 84 628.5 0 84 94 110 628.5 0 110 95 89 628.5 0 89 96 88 628.5 0 88 97 104 628.5 0 104 98 76 628.5 0 76 99 111 628.5 0 111 100 914 628.5 0 914 101 1752 628.5 0 1752 102 1891 628.5 0 1891 103 77 628.5 0 77 104 59 628.5 0 59 105 46 628.5 0 46 106 53 628.5 0 53 107 63 628.5 0 63 108 31 628.5 0 31 109 59 628.5 0 59 110 89 628.5 0 89 111 83 628.5 0 83 112 60 628.5 0 60 113 99 628.5 0 99 114 104 628.5 0 104 115 80 628.5 0 80 116 67 628.5 0 67 117 63 628.5 0 63 118 98 628.5 0 98 119 85 628.5 0 85 120 78 628.5 0 78 121 71 628.5 0 71 122 101 628.5 0 101 123 97 628.5 0 97 124 113 628.5 0 113 125 76 628.5 0 76 126 76 628.5 0 76 127 68 628.5 0 68 128 77 628.5 0 77 129 55 628.5 0 55 130 100 628.5 0 100 131 101 628.5 0 101 132 416 628.5 0 416 133 423 628.5 0 423 134 395 628.5 0 395 135 99 628.5 0 99 136 499 628.5 0 499 137 742 628.5 0 742 138 388 628.5 0 388 139 152 628.5 0 152 140 86 628.5 0 86 141 72 628.5 0 72 142 76 628.5 0 76 143 72 628.5 0 72 144 94 628.5 0 94 145 98 628.5 0 98 146 99 628.5 0 99 147 66 628.5 0 66 148 78 628.5 0 78 149 68 628.5 0 68 150 96 628.5 0 96 151 101 628.5 0 101 152 114 628.5 0 114 153 140 628.5 0 140 154 116 628.5 0 116 155 85 628.5 0 85 156 80 628.5 0 80 157 174 628.5 0 174 158 225 628.5 0 225 159 325 628.5 0 325 160 138 628.5 0 138 161 93 628.5 0 93 162 115 628.5 0 115 163 145 628.5 0 145 164 152 628.5 0 152 165 99 628.5 0 99 166 125 628.5 0 125 167 174 628.5 0 174 168 153 628.5 0 153 169 213 628.5 0 213 170 185 628.5 0 185 171 114 628.5 0 114 172 132 628.5 0 132 173 98 628.5 0 98 174 111 628.5 0 111 175 104 628.5 0 104 176 263 628.5 0 263 177 403 628.5 0 403 178 712 628.5 0 712 179 794 628.5 0 794 180 258 628.5 0 258 181 219 628.5 0 219 182 660 628.5 0 660 183 714 628.5 0 714 184 1231 628.5 0 1231 185 223 628.5 0 223 186 144 628.5 0 144 187 145 628.5 0 145 188 117 628.5 0 117 189 198 628.5 0 198 190 169 628.5 0 169 191 123 628.5 0 123 192 152 628.5 0 152 193 183 628.5 0 183 194 127 628.5 0 127 195 99 628.5 0 99 196 143 628.5 0 143 197 214 628.5 0 214 198 306 628.5 0 306 199 256 628.5 0 256 200 150 628.5 0 150 201 135 628.5 0 135 202 929 628.5 0 929 203 1262 628.5 0 1262 204 1646 628.5 0 1646 205 1641 628.5 0 1641 206 87 628.5 0 87 207 88 628.5 0 88 208 83 628.5 0 83 209 90 628.5 0 90 210 124 628.5 0 124 211 220 628.5 0 220 212 182 628.5 0 182 213 158 628.5 0 158 214 91 628.5 0 91 215 99 628.5 0 99 216 111 628.5 0 111 217 155 628.5 0 155 218 148 628.5 0 148 219 137 628.5 0 137 220 96 628.5 0 96 221 129 628.5 0 129 222 409 628.5 0 409 223 892 628.5 0 892 224 894 628.5 0 894 225 551 628.5 0 551 226 223 628.5 0 223 227 161 628.5 0 161 228 140 628.5 0 140 229 177 628.5 0 177 230 95 628.5 0 95 231 107 628.5 0 107 232 95 628.5 0 95 233 87 628.5 0 87 234 113 628.5 0 113 235 96 628.5 0 96 236 73 628.5 0 73 237 80 628.5 0 80 238 125 628.5 0 125 239 94 628.5 0 94 240 138 628.5 0 138 241 117 628.5 0 117 242 451 628.5 0 451 243 715 628.5 0 715 244 834 628.5 0 834 245 795 628.5 0 795 246 134 628.5 0 134 247 87 628.5 0 87 248 83 628.5 0 83 249 84 628.5 0 84 250 101 628.5 0 101 251 170 628.5 0 170 252 196 628.5 0 196 253 186 628.5 0 186 254 109 628.5 0 109 255 704 628.5 0 704 256 272 628.5 0 272 257 412 628.5 0 412 258 194 628.5 0 194 259 165 628.5 0 165 260 117 628.5 0 117 261 101 628.5 0 101 262 159 628.5 0 159 263 194 628.5 0 194 264 256 628.5 0 256 265 601 628.5 0 601 266 336 628.5 0 336 267 294 628.5 0 294 268 135 628.5 0 135 269 422 628.5 0 422 270 330 628.5 0 330 271 654 628.5 0 654 272 798 628.5 0 798 273 473 628.5 0 473 274 202 628.5 0 202 275 226 628.5 0 226 276 229 628.5 0 229 277 269 628.5 0 269 278 408 628.5 0 408 279 455 628.5 0 455 280 124 628.5 0 124 281 146 628.5 0 146 282 97 628.5 0 97 283 184 628.5 0 184 284 182 628.5 0 182 285 232 628.5 0 232 286 207 628.5 0 207 287 153 628.5 0 153 288 151 628.5 0 151 289 125 628.5 0 125 290 122 628.5 0 122 291 299 628.5 0 299 292 491 628.5 0 491 293 462 628.5 0 462 294 411 628.5 0 411 295 112 628.5 0 112 296 97 628.5 0 97 297 115 628.5 0 115 298 190 628.5 0 190 299 179 628.5 0 179 300 264 628.5 0 264 301 180 628.5 0 180 Finished in 3.91 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 90,572 Reads with adapters: 23,314 (25.7%) Reads that were too short: 237 (0.3%) Reads written (passing filters): 23,077 (25.5%) Total basepairs processed: 21,939,142 bp Total written (filtered): 6,318,710 bp (28.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23314 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 35 0.0 3 0 0 12 23 16 321 0.0 3 0 252 37 32 17 21115 0.0 3 18629 1890 461 135 18 80 0.0 3 12 45 15 8 19 142 0.0 3 8 18 66 50 20 1063 0.0 3 913 77 21 52 21 6 0.0 3 1 3 0 2 22 14 0.0 3 0 0 4 10 23 3 0.0 3 0 0 0 3 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 29 3 0.0 3 0 0 0 3 32 4 0.0 3 0 1 0 3 33 1 0.0 3 0 0 0 1 34 24 0.0 3 0 0 1 23 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 37 9 0.0 3 0 0 1 8 38 2 0.0 3 0 0 0 2 40 7 0.0 3 0 0 0 7 42 4 0.0 3 0 0 1 3 46 1 0.0 3 0 0 0 1 47 3 0.0 3 0 0 1 2 48 2 0.0 3 0 0 0 2 49 4 0.0 3 0 0 0 4 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 55 31 0.0 3 0 0 0 31 58 9 0.0 3 0 0 1 8 59 1 0.0 3 0 0 0 1 60 6 0.0 3 0 0 1 5 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 69 1 0.0 3 0 0 0 1 71 11 0.0 3 0 0 0 11 73 2 0.0 3 0 0 0 2 74 5 0.0 3 0 0 0 5 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 1 78 3 0.0 3 0 0 0 3 79 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 6 0.0 3 0 0 0 6 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 89 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 91 119 0.0 3 0 0 4 115 92 8 0.0 3 0 0 0 8 93 1 0.0 3 0 0 0 1 94 15 0.0 3 0 0 0 15 95 3 0.0 3 0 0 0 3 99 4 0.0 3 0 0 0 4 100 5 0.0 3 0 0 0 5 101 2 0.0 3 0 0 0 2 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 7 0.0 3 0 0 0 7 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 6 0.0 3 0 0 0 6 110 2 0.0 3 0 0 0 2 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 114 4 0.0 3 0 0 0 4 116 1 0.0 3 0 0 0 1 117 4 0.0 3 0 0 0 4 118 3 0.0 3 0 0 0 3 119 3 0.0 3 0 0 0 3 122 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 130 4 0.0 3 0 0 0 4 131 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 4 0.0 3 0 0 0 4 136 1 0.0 3 0 0 0 1 137 3 0.0 3 0 0 0 3 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 144 3 0.0 3 0 0 1 2 146 6 0.0 3 0 0 0 6 147 5 0.0 3 0 0 0 5 148 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 150 3 0.0 3 0 0 0 3 151 6 0.0 3 0 0 0 6 154 1 0.0 3 0 0 0 1 156 9 0.0 3 0 0 1 8 157 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 3 0.0 3 0 0 0 3 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 6 0.0 3 0 0 0 6 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 8 0.0 3 0 0 0 8 193 4 0.0 3 0 0 0 4 194 4 0.0 3 0 0 0 4 200 1 0.0 3 0 0 0 1 205 7 0.0 3 0 0 0 7 206 1 0.0 3 0 0 1 211 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 219 3 0.0 3 0 0 0 3 220 5 0.0 3 0 0 0 5 221 4 0.0 3 0 0 0 4 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 281 6 0.0 3 0 0 1 5 282 1 0.0 3 0 0 0 1 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 23,077 Reads with adapters: 142 (0.6%) Reads written (passing filters): 23,077 (100.0%) Total basepairs processed: 6,318,710 bp Total written (filtered): 6,318,241 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 142 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 56.3% C: 15.5% G: 21.1% T: 7.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 360.6 0 107 4 27 90.1 0 27 5 8 22.5 0 8