This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 69.53 s (27 us/read; 2.22 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 98,932 (3.8%) Reads that were too short: 12,256 (0.5%) Reads written (passing filters): 86,676 (3.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 22,108,935 bp (2.9%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 98932 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1421 628.5 0 1421 7 499 628.5 0 499 8 21818 628.5 0 21818 9 23735 628.5 0 23735 10 98 628.5 0 98 11 48 628.5 0 48 12 66 628.5 0 66 13 67 628.5 0 67 14 53 628.5 0 53 15 56 628.5 0 56 16 56 628.5 0 56 17 66 628.5 0 66 18 67 628.5 0 67 19 75 628.5 0 75 20 74 628.5 0 74 21 40 628.5 0 40 22 49 628.5 0 49 23 47 628.5 0 47 24 41 628.5 0 41 25 58 628.5 0 58 26 43 628.5 0 43 27 57 628.5 0 57 28 59 628.5 0 59 29 38 628.5 0 38 30 63 628.5 0 63 31 67 628.5 0 67 32 57 628.5 0 57 33 63 628.5 0 63 34 63 628.5 0 63 35 71 628.5 0 71 36 68 628.5 0 68 37 53 628.5 0 53 38 41 628.5 0 41 39 65 628.5 0 65 40 54 628.5 0 54 41 66 628.5 0 66 42 205 628.5 0 205 43 68 628.5 0 68 44 53 628.5 0 53 45 62 628.5 0 62 46 63 628.5 0 63 47 59 628.5 0 59 48 70 628.5 0 70 49 58 628.5 0 58 50 53 628.5 0 53 51 99 628.5 0 99 52 96 628.5 0 96 53 65 628.5 0 65 54 105 628.5 0 105 55 79 628.5 0 79 56 78 628.5 0 78 57 115 628.5 0 115 58 84 628.5 0 84 59 71 628.5 0 71 60 61 628.5 0 61 61 53 628.5 0 53 62 91 628.5 0 91 63 103 628.5 0 103 64 129 628.5 0 129 65 189 628.5 0 189 66 175 628.5 0 175 67 399 628.5 0 399 68 582 628.5 0 582 69 8408 628.5 0 8408 70 3501 628.5 0 3501 71 9013 628.5 0 9013 72 72 628.5 0 72 73 56 628.5 0 56 74 64 628.5 0 64 75 54 628.5 0 54 76 59 628.5 0 59 77 59 628.5 0 59 78 54 628.5 0 54 79 62 628.5 0 62 80 192 628.5 0 192 81 96 628.5 0 96 82 156 628.5 0 156 83 69 628.5 0 69 84 51 628.5 0 51 85 47 628.5 0 47 86 70 628.5 0 70 87 78 628.5 0 78 88 69 628.5 0 69 89 73 628.5 0 73 90 57 628.5 0 57 91 67 628.5 0 67 92 68 628.5 0 68 93 87 628.5 0 87 94 69 628.5 0 69 95 79 628.5 0 79 96 66 628.5 0 66 97 71 628.5 0 71 98 81 628.5 0 81 99 60 628.5 0 60 100 170 628.5 0 170 101 316 628.5 0 316 102 359 628.5 0 359 103 78 628.5 0 78 104 47 628.5 0 47 105 43 628.5 0 43 106 59 628.5 0 59 107 62 628.5 0 62 108 48 628.5 0 48 109 63 628.5 0 63 110 68 628.5 0 68 111 72 628.5 0 72 112 54 628.5 0 54 113 72 628.5 0 72 114 92 628.5 0 92 115 75 628.5 0 75 116 64 628.5 0 64 117 69 628.5 0 69 118 57 628.5 0 57 119 54 628.5 0 54 120 62 628.5 0 62 121 49 628.5 0 49 122 49 628.5 0 49 123 62 628.5 0 62 124 66 628.5 0 66 125 66 628.5 0 66 126 77 628.5 0 77 127 56 628.5 0 56 128 61 628.5 0 61 129 55 628.5 0 55 130 93 628.5 0 93 131 69 628.5 0 69 132 228 628.5 0 228 133 199 628.5 0 199 134 193 628.5 0 193 135 64 628.5 0 64 136 193 628.5 0 193 137 337 628.5 0 337 138 153 628.5 0 153 139 103 628.5 0 103 140 71 628.5 0 71 141 71 628.5 0 71 142 56 628.5 0 56 143 65 628.5 0 65 144 62 628.5 0 62 145 66 628.5 0 66 146 53 628.5 0 53 147 72 628.5 0 72 148 78 628.5 0 78 149 54 628.5 0 54 150 75 628.5 0 75 151 72 628.5 0 72 152 84 628.5 0 84 153 81 628.5 0 81 154 89 628.5 0 89 155 73 628.5 0 73 156 78 628.5 0 78 157 110 628.5 0 110 158 99 628.5 0 99 159 169 628.5 0 169 160 72 628.5 0 72 161 94 628.5 0 94 162 73 628.5 0 73 163 84 628.5 0 84 164 80 628.5 0 80 165 61 628.5 0 61 166 103 628.5 0 103 167 129 628.5 0 129 168 84 628.5 0 84 169 95 628.5 0 95 170 111 628.5 0 111 171 68 628.5 0 68 172 97 628.5 0 97 173 95 628.5 0 95 174 76 628.5 0 76 175 98 628.5 0 98 176 139 628.5 0 139 177 206 628.5 0 206 178 367 628.5 0 367 179 410 628.5 0 410 180 136 628.5 0 136 181 102 628.5 0 102 182 251 628.5 0 251 183 263 628.5 0 263 184 401 628.5 0 401 185 115 628.5 0 115 186 89 628.5 0 89 187 81 628.5 0 81 188 103 628.5 0 103 189 97 628.5 0 97 190 103 628.5 0 103 191 90 628.5 0 90 192 98 628.5 0 98 193 110 628.5 0 110 194 90 628.5 0 90 195 72 628.5 0 72 196 87 628.5 0 87 197 108 628.5 0 108 198 107 628.5 0 107 199 96 628.5 0 96 200 85 628.5 0 85 201 80 628.5 0 80 202 263 628.5 0 263 203 375 628.5 0 375 204 319 628.5 0 319 205 328 628.5 0 328 206 59 628.5 0 59 207 55 628.5 0 55 208 83 628.5 0 83 209 82 628.5 0 82 210 84 628.5 0 84 211 129 628.5 0 129 212 111 628.5 0 111 213 110 628.5 0 110 214 77 628.5 0 77 215 81 628.5 0 81 216 70 628.5 0 70 217 81 628.5 0 81 218 86 628.5 0 86 219 80 628.5 0 80 220 67 628.5 0 67 221 68 628.5 0 68 222 113 628.5 0 113 223 254 628.5 0 254 224 226 628.5 0 226 225 183 628.5 0 183 226 91 628.5 0 91 227 125 628.5 0 125 228 118 628.5 0 118 229 152 628.5 0 152 230 72 628.5 0 72 231 83 628.5 0 83 232 75 628.5 0 75 233 66 628.5 0 66 234 65 628.5 0 65 235 66 628.5 0 66 236 52 628.5 0 52 237 68 628.5 0 68 238 71 628.5 0 71 239 57 628.5 0 57 240 79 628.5 0 79 241 63 628.5 0 63 242 129 628.5 0 129 243 176 628.5 0 176 244 178 628.5 0 178 245 191 628.5 0 191 246 101 628.5 0 101 247 94 628.5 0 94 248 42 628.5 0 42 249 66 628.5 0 66 250 125 628.5 0 125 251 99 628.5 0 99 252 99 628.5 0 99 253 85 628.5 0 85 254 84 628.5 0 84 255 192 628.5 0 192 256 122 628.5 0 122 257 122 628.5 0 122 258 136 628.5 0 136 259 90 628.5 0 90 260 78 628.5 0 78 261 72 628.5 0 72 262 103 628.5 0 103 263 122 628.5 0 122 264 100 628.5 0 100 265 216 628.5 0 216 266 154 628.5 0 154 267 122 628.5 0 122 268 76 628.5 0 76 269 265 628.5 0 265 270 165 628.5 0 165 271 261 628.5 0 261 272 279 628.5 0 279 273 148 628.5 0 148 274 112 628.5 0 112 275 145 628.5 0 145 276 125 628.5 0 125 277 138 628.5 0 138 278 149 628.5 0 149 279 163 628.5 0 163 280 63 628.5 0 63 281 80 628.5 0 80 282 71 628.5 0 71 283 129 628.5 0 129 284 109 628.5 0 109 285 115 628.5 0 115 286 120 628.5 0 120 287 84 628.5 0 84 288 87 628.5 0 87 289 98 628.5 0 98 290 78 628.5 0 78 291 134 628.5 0 134 292 177 628.5 0 177 293 151 628.5 0 151 294 147 628.5 0 147 295 67 628.5 0 67 296 71 628.5 0 71 297 98 628.5 0 98 298 139 628.5 0 139 299 157 628.5 0 157 300 176 628.5 0 176 301 94 628.5 0 94 Finished in 4.26 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 86,676 Reads with adapters: 25,390 (29.3%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 25,378 (29.3%) Total basepairs processed: 22,108,935 bp Total written (filtered): 6,827,821 bp (30.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 25390 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 9 19 438 0.0 3 0 430 7 1 20 24127 0.0 4 23691 414 19 1 2 21 53 0.0 4 15 34 4 22 5 0.0 4 2 0 3 23 24 0.0 4 1 0 0 20 3 24 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 124 1 0.0 4 1 126 6 0.0 4 0 0 0 0 6 127 569 0.0 4 0 0 0 0 569 128 5 0.0 4 0 0 0 0 5 129 1 0.0 4 0 0 0 0 1 130 137 0.0 4 0 0 0 0 137 153 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 249 1 0.0 4 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.89 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 25,378 Reads with adapters: 3,169 (12.5%) Reads written (passing filters): 25,378 (100.0%) Total basepairs processed: 6,827,821 bp Total written (filtered): 6,783,284 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3169 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.5% G: 92.4% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 396.5 0 4 6 2 6.2 0 2 7 2 1.5 0 2 9 1130 0.1 0 45 1085 10 1719 0.0 1 59 1660 11 241 0.0 1 2 239 12 1 0.0 1 0 1 13 3 0.0 1 0 3 150 1 0.0 1 0 1 169 6 0.0 1 6 170 2 0.0 1 2 220 1 0.0 1 1 222 1 0.0 1 1 223 54 0.0 1 48 6 224 1 0.0 1 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.