This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.42 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 267,095 (10.4%) Reads that were too short: 14,392 (0.6%) Reads written (passing filters): 252,703 (9.8%) Total basepairs processed: 774,927,510 bp Total written (filtered): 71,689,211 bp (9.3%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 267095 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3071 628.5 0 3071 7 106896 628.5 0 106896 8 6169 628.5 0 6169 9 111160 628.5 0 111160 10 274 628.5 0 274 11 78 628.5 0 78 12 65 628.5 0 65 13 90 628.5 0 90 14 118 628.5 0 118 15 93 628.5 0 93 16 79 628.5 0 79 17 80 628.5 0 80 18 76 628.5 0 76 19 100 628.5 0 100 20 126 628.5 0 126 21 126 628.5 0 126 22 188 628.5 0 188 23 456 628.5 0 456 24 350 628.5 0 350 25 523 628.5 0 523 26 74 628.5 0 74 27 71 628.5 0 71 28 71 628.5 0 71 29 83 628.5 0 83 30 82 628.5 0 82 31 62 628.5 0 62 32 85 628.5 0 85 33 81 628.5 0 81 34 93 628.5 0 93 35 125 628.5 0 125 36 107 628.5 0 107 37 80 628.5 0 80 38 79 628.5 0 79 39 78 628.5 0 78 40 80 628.5 0 80 41 57 628.5 0 57 42 68 628.5 0 68 43 66 628.5 0 66 44 70 628.5 0 70 45 78 628.5 0 78 46 65 628.5 0 65 47 62 628.5 0 62 48 62 628.5 0 62 49 66 628.5 0 66 50 62 628.5 0 62 51 46 628.5 0 46 52 59 628.5 0 59 53 59 628.5 0 59 54 66 628.5 0 66 55 71 628.5 0 71 56 82 628.5 0 82 57 58 628.5 0 58 58 65 628.5 0 65 59 62 628.5 0 62 60 75 628.5 0 75 61 62 628.5 0 62 62 54 628.5 0 54 63 78 628.5 0 78 64 73 628.5 0 73 65 73 628.5 0 73 66 74 628.5 0 74 67 74 628.5 0 74 68 70 628.5 0 70 69 62 628.5 0 62 70 73 628.5 0 73 71 98 628.5 0 98 72 64 628.5 0 64 73 270 628.5 0 270 74 99 628.5 0 99 75 70 628.5 0 70 76 54 628.5 0 54 77 56 628.5 0 56 78 72 628.5 0 72 79 62 628.5 0 62 80 74 628.5 0 74 81 97 628.5 0 97 82 105 628.5 0 105 83 77 628.5 0 77 84 115 628.5 0 115 85 431 628.5 0 431 86 111 628.5 0 111 87 114 628.5 0 114 88 971 628.5 0 971 89 1690 628.5 0 1690 90 796 628.5 0 796 91 57 628.5 0 57 92 53 628.5 0 53 93 55 628.5 0 55 94 91 628.5 0 91 95 82 628.5 0 82 96 268 628.5 0 268 97 289 628.5 0 289 98 223 628.5 0 223 99 125 628.5 0 125 100 163 628.5 0 163 101 909 628.5 0 909 102 457 628.5 0 457 103 712 628.5 0 712 104 67 628.5 0 67 105 95 628.5 0 95 106 77 628.5 0 77 107 44 628.5 0 44 108 50 628.5 0 50 109 76 628.5 0 76 110 119 628.5 0 119 111 97 628.5 0 97 112 87 628.5 0 87 113 82 628.5 0 82 114 85 628.5 0 85 115 83 628.5 0 83 116 57 628.5 0 57 117 85 628.5 0 85 118 67 628.5 0 67 119 76 628.5 0 76 120 50 628.5 0 50 121 63 628.5 0 63 122 84 628.5 0 84 123 94 628.5 0 94 124 75 628.5 0 75 125 244 628.5 0 244 126 135 628.5 0 135 127 1244 628.5 0 1244 128 425 628.5 0 425 129 477 628.5 0 477 130 95 628.5 0 95 131 85 628.5 0 85 132 59 628.5 0 59 133 93 628.5 0 93 134 75 628.5 0 75 135 88 628.5 0 88 136 67 628.5 0 67 137 65 628.5 0 65 138 77 628.5 0 77 139 58 628.5 0 58 140 85 628.5 0 85 141 99 628.5 0 99 142 76 628.5 0 76 143 103 628.5 0 103 144 527 628.5 0 527 145 96 628.5 0 96 146 85 628.5 0 85 147 196 628.5 0 196 148 274 628.5 0 274 149 195 628.5 0 195 150 52 628.5 0 52 151 59 628.5 0 59 152 77 628.5 0 77 153 79 628.5 0 79 154 56 628.5 0 56 155 52 628.5 0 52 156 68 628.5 0 68 157 61 628.5 0 61 158 87 628.5 0 87 159 86 628.5 0 86 160 52 628.5 0 52 161 69 628.5 0 69 162 47 628.5 0 47 163 51 628.5 0 51 164 52 628.5 0 52 165 47 628.5 0 47 166 35 628.5 0 35 167 68 628.5 0 68 168 72 628.5 0 72 169 84 628.5 0 84 170 57 628.5 0 57 171 76 628.5 0 76 172 48 628.5 0 48 173 36 628.5 0 36 174 41 628.5 0 41 175 52 628.5 0 52 176 97 628.5 0 97 177 82 628.5 0 82 178 82 628.5 0 82 179 51 628.5 0 51 180 52 628.5 0 52 181 78 628.5 0 78 182 103 628.5 0 103 183 65 628.5 0 65 184 49 628.5 0 49 185 47 628.5 0 47 186 63 628.5 0 63 187 67 628.5 0 67 188 50 628.5 0 50 189 59 628.5 0 59 190 78 628.5 0 78 191 39 628.5 0 39 192 76 628.5 0 76 193 85 628.5 0 85 194 95 628.5 0 95 195 252 628.5 0 252 196 174 628.5 0 174 197 102 628.5 0 102 198 56 628.5 0 56 199 56 628.5 0 56 200 97 628.5 0 97 201 177 628.5 0 177 202 151 628.5 0 151 203 109 628.5 0 109 204 99 628.5 0 99 205 84 628.5 0 84 206 72 628.5 0 72 207 134 628.5 0 134 208 123 628.5 0 123 209 198 628.5 0 198 210 97 628.5 0 97 211 78 628.5 0 78 212 68 628.5 0 68 213 82 628.5 0 82 214 95 628.5 0 95 215 109 628.5 0 109 216 86 628.5 0 86 217 88 628.5 0 88 218 367 628.5 0 367 219 75 628.5 0 75 220 66 628.5 0 66 221 64 628.5 0 64 222 79 628.5 0 79 223 125 628.5 0 125 224 112 628.5 0 112 225 79 628.5 0 79 226 81 628.5 0 81 227 61 628.5 0 61 228 74 628.5 0 74 229 75 628.5 0 75 230 78 628.5 0 78 231 113 628.5 0 113 232 121 628.5 0 121 233 85 628.5 0 85 234 107 628.5 0 107 235 128 628.5 0 128 236 116 628.5 0 116 237 95 628.5 0 95 238 159 628.5 0 159 239 267 628.5 0 267 240 262 628.5 0 262 241 194 628.5 0 194 242 136 628.5 0 136 243 71 628.5 0 71 244 62 628.5 0 62 245 60 628.5 0 60 246 68 628.5 0 68 247 74 628.5 0 74 248 75 628.5 0 75 249 81 628.5 0 81 250 81 628.5 0 81 251 65 628.5 0 65 252 64 628.5 0 64 253 62 628.5 0 62 254 91 628.5 0 91 255 106 628.5 0 106 256 144 628.5 0 144 257 92 628.5 0 92 258 111 628.5 0 111 259 1080 628.5 0 1080 260 1409 628.5 0 1409 261 1043 628.5 0 1043 262 516 628.5 0 516 263 256 628.5 0 256 264 51 628.5 0 51 265 61 628.5 0 61 266 72 628.5 0 72 267 59 628.5 0 59 268 75 628.5 0 75 269 76 628.5 0 76 270 50 628.5 0 50 271 45 628.5 0 45 272 71 628.5 0 71 273 89 628.5 0 89 274 76 628.5 0 76 275 90 628.5 0 90 276 71 628.5 0 71 277 61 628.5 0 61 278 77 628.5 0 77 279 320 628.5 0 320 280 382 628.5 0 382 281 596 628.5 0 596 282 74 628.5 0 74 283 72 628.5 0 72 284 77 628.5 0 77 285 39 628.5 0 39 286 70 628.5 0 70 287 42 628.5 0 42 288 72 628.5 0 72 289 71 628.5 0 71 290 66 628.5 0 66 291 109 628.5 0 109 292 124 628.5 0 124 293 139 628.5 0 139 294 100 628.5 0 100 295 105 628.5 0 105 296 124 628.5 0 124 297 62 628.5 0 62 298 74 628.5 0 74 299 70 628.5 0 70 300 86 628.5 0 86 301 91 628.5 0 91 Finished in 11.22 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 252,703 Reads with adapters: 118,940 (47.1%) Reads that were too short: 154 (0.1%) Reads written (passing filters): 118,786 (47.0%) Total basepairs processed: 71,689,211 bp Total written (filtered): 32,637,828 bp (45.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 118940 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 38 0.0 2 0 0 0 38 15 98 0.0 3 0 0 77 21 16 1242 0.0 3 0 1026 147 69 17 116319 0.0 3 105462 8738 1750 369 18 291 0.0 3 8 235 30 18 19 313 0.0 3 5 1 172 135 20 229 0.0 3 6 3 4 216 21 1 0.0 3 0 0 0 1 22 1 0.0 3 1 23 1 0.0 3 1 31 1 0.0 3 0 0 1 33 1 0.0 3 1 34 5 0.0 3 0 0 0 5 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 5 0.0 3 0 0 4 1 42 2 0.0 3 1 0 0 1 44 8 0.0 3 0 0 0 8 46 3 0.0 3 0 0 0 3 47 3 0.0 3 0 0 0 3 48 3 0.0 3 0 0 1 2 50 1 0.0 3 0 0 1 56 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 1 3 62 2 0.0 3 0 0 0 2 64 53 0.0 3 0 0 0 53 65 3 0.0 3 0 0 0 3 66 13 0.0 3 0 0 0 13 67 3 0.0 3 0 0 0 3 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 75 11 0.0 3 0 0 0 11 78 2 0.0 3 0 0 0 2 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 86 2 0.0 3 1 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 4 0.0 3 0 0 0 4 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 8 0.0 3 0 0 0 8 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 40 0.0 3 0 0 2 38 104 17 0.0 3 0 0 0 17 106 5 0.0 3 0 0 0 5 111 4 0.0 3 0 0 0 4 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 1 117 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 1 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 4 0.0 3 0 0 0 4 164 3 0.0 3 0 0 0 3 166 3 0.0 3 0 0 1 2 167 5 0.0 3 0 0 0 5 168 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 178 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 4 0.0 3 0 0 1 3 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 194 12 0.0 3 0 0 1 11 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 200 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 3 0.0 3 0 0 0 3 206 4 0.0 3 0 0 0 4 210 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 225 2 0.0 3 0 0 0 2 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 0 2 240 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 3 0.0 3 0 0 0 3 279 8 0.0 3 0 0 0 8 280 8 0.0 3 0 0 1 7 281 14 0.0 3 0 0 0 14 282 6 0.0 3 0 0 0 6 283 2 0.0 3 0 0 0 2 287 3 0.0 3 0 0 0 3 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 294 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.12 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 118,786 Reads with adapters: 2,426 (2.0%) Reads written (passing filters): 118,786 (100.0%) Total basepairs processed: 32,637,828 bp Total written (filtered): 32,630,002 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2426 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.1% C: 11.3% G: 36.8% T: 1.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2011 1856.0 0 2011 4 409 464.0 0 409 5 3 116.0 0 3 6 1 29.0 0 1 12 1 0.0 1 0 1 124 1 0.0 2 0 0 1