This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.08 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 269,361 (10.5%) Reads that were too short: 7,553 (0.3%) Reads written (passing filters): 261,808 (10.2%) Total basepairs processed: 774,927,510 bp Total written (filtered): 75,329,830 bp (9.7%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 269361 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3041 628.5 0 3041 7 130640 628.5 0 130640 8 3663 628.5 0 3663 9 109001 628.5 0 109001 10 193 628.5 0 193 11 58 628.5 0 58 12 37 628.5 0 37 13 40 628.5 0 40 14 41 628.5 0 41 15 38 628.5 0 38 16 37 628.5 0 37 17 48 628.5 0 48 18 40 628.5 0 40 19 41 628.5 0 41 20 45 628.5 0 45 21 53 628.5 0 53 22 83 628.5 0 83 23 461 628.5 0 461 24 258 628.5 0 258 25 570 628.5 0 570 26 28 628.5 0 28 27 29 628.5 0 29 28 27 628.5 0 27 29 32 628.5 0 32 30 40 628.5 0 40 31 39 628.5 0 39 32 39 628.5 0 39 33 35 628.5 0 35 34 38 628.5 0 38 35 34 628.5 0 34 36 35 628.5 0 35 37 39 628.5 0 39 38 32 628.5 0 32 39 31 628.5 0 31 40 44 628.5 0 44 41 36 628.5 0 36 42 35 628.5 0 35 43 35 628.5 0 35 44 39 628.5 0 39 45 23 628.5 0 23 46 35 628.5 0 35 47 23 628.5 0 23 48 27 628.5 0 27 49 21 628.5 0 21 50 35 628.5 0 35 51 35 628.5 0 35 52 30 628.5 0 30 53 23 628.5 0 23 54 35 628.5 0 35 55 37 628.5 0 37 56 30 628.5 0 30 57 28 628.5 0 28 58 30 628.5 0 30 59 22 628.5 0 22 60 27 628.5 0 27 61 32 628.5 0 32 62 20 628.5 0 20 63 22 628.5 0 22 64 30 628.5 0 30 65 18 628.5 0 18 66 34 628.5 0 34 67 23 628.5 0 23 68 33 628.5 0 33 69 30 628.5 0 30 70 32 628.5 0 32 71 54 628.5 0 54 72 41 628.5 0 41 73 267 628.5 0 267 74 52 628.5 0 52 75 38 628.5 0 38 76 33 628.5 0 33 77 25 628.5 0 25 78 40 628.5 0 40 79 24 628.5 0 24 80 35 628.5 0 35 81 59 628.5 0 59 82 41 628.5 0 41 83 39 628.5 0 39 84 59 628.5 0 59 85 398 628.5 0 398 86 81 628.5 0 81 87 61 628.5 0 61 88 528 628.5 0 528 89 1421 628.5 0 1421 90 497 628.5 0 497 91 19 628.5 0 19 92 22 628.5 0 22 93 21 628.5 0 21 94 31 628.5 0 31 95 22 628.5 0 22 96 60 628.5 0 60 97 60 628.5 0 60 98 41 628.5 0 41 99 53 628.5 0 53 100 68 628.5 0 68 101 554 628.5 0 554 102 352 628.5 0 352 103 493 628.5 0 493 104 43 628.5 0 43 105 45 628.5 0 45 106 45 628.5 0 45 107 22 628.5 0 22 108 36 628.5 0 36 109 22 628.5 0 22 110 51 628.5 0 51 111 51 628.5 0 51 112 34 628.5 0 34 113 51 628.5 0 51 114 42 628.5 0 42 115 32 628.5 0 32 116 21 628.5 0 21 117 51 628.5 0 51 118 29 628.5 0 29 119 34 628.5 0 34 120 33 628.5 0 33 121 32 628.5 0 32 122 44 628.5 0 44 123 29 628.5 0 29 124 45 628.5 0 45 125 154 628.5 0 154 126 61 628.5 0 61 127 1188 628.5 0 1188 128 371 628.5 0 371 129 454 628.5 0 454 130 38 628.5 0 38 131 30 628.5 0 30 132 34 628.5 0 34 133 45 628.5 0 45 134 35 628.5 0 35 135 36 628.5 0 36 136 26 628.5 0 26 137 26 628.5 0 26 138 23 628.5 0 23 139 16 628.5 0 16 140 37 628.5 0 37 141 42 628.5 0 42 142 40 628.5 0 40 143 43 628.5 0 43 144 638 628.5 0 638 145 69 628.5 0 69 146 50 628.5 0 50 147 184 628.5 0 184 148 245 628.5 0 245 149 151 628.5 0 151 150 26 628.5 0 26 151 42 628.5 0 42 152 25 628.5 0 25 153 33 628.5 0 33 154 19 628.5 0 19 155 23 628.5 0 23 156 32 628.5 0 32 157 40 628.5 0 40 158 32 628.5 0 32 159 27 628.5 0 27 160 30 628.5 0 30 161 39 628.5 0 39 162 28 628.5 0 28 163 26 628.5 0 26 164 26 628.5 0 26 165 22 628.5 0 22 166 21 628.5 0 21 167 41 628.5 0 41 168 22 628.5 0 22 169 46 628.5 0 46 170 25 628.5 0 25 171 17 628.5 0 17 172 23 628.5 0 23 173 12 628.5 0 12 174 16 628.5 0 16 175 18 628.5 0 18 176 38 628.5 0 38 177 34 628.5 0 34 178 32 628.5 0 32 179 27 628.5 0 27 180 25 628.5 0 25 181 27 628.5 0 27 182 35 628.5 0 35 183 30 628.5 0 30 184 29 628.5 0 29 185 26 628.5 0 26 186 37 628.5 0 37 187 36 628.5 0 36 188 24 628.5 0 24 189 29 628.5 0 29 190 40 628.5 0 40 191 17 628.5 0 17 192 29 628.5 0 29 193 39 628.5 0 39 194 46 628.5 0 46 195 56 628.5 0 56 196 41 628.5 0 41 197 26 628.5 0 26 198 17 628.5 0 17 199 25 628.5 0 25 200 45 628.5 0 45 201 80 628.5 0 80 202 51 628.5 0 51 203 39 628.5 0 39 204 32 628.5 0 32 205 37 628.5 0 37 206 30 628.5 0 30 207 32 628.5 0 32 208 58 628.5 0 58 209 96 628.5 0 96 210 29 628.5 0 29 211 38 628.5 0 38 212 31 628.5 0 31 213 35 628.5 0 35 214 43 628.5 0 43 215 37 628.5 0 37 216 38 628.5 0 38 217 38 628.5 0 38 218 382 628.5 0 382 219 33 628.5 0 33 220 26 628.5 0 26 221 26 628.5 0 26 222 35 628.5 0 35 223 45 628.5 0 45 224 40 628.5 0 40 225 43 628.5 0 43 226 38 628.5 0 38 227 32 628.5 0 32 228 28 628.5 0 28 229 35 628.5 0 35 230 36 628.5 0 36 231 45 628.5 0 45 232 32 628.5 0 32 233 30 628.5 0 30 234 65 628.5 0 65 235 46 628.5 0 46 236 132 628.5 0 132 237 68 628.5 0 68 238 69 628.5 0 69 239 132 628.5 0 132 240 101 628.5 0 101 241 60 628.5 0 60 242 35 628.5 0 35 243 46 628.5 0 46 244 40 628.5 0 40 245 19 628.5 0 19 246 45 628.5 0 45 247 45 628.5 0 45 248 48 628.5 0 48 249 50 628.5 0 50 250 46 628.5 0 46 251 40 628.5 0 40 252 32 628.5 0 32 253 30 628.5 0 30 254 57 628.5 0 57 255 77 628.5 0 77 256 81 628.5 0 81 257 48 628.5 0 48 258 67 628.5 0 67 259 820 628.5 0 820 260 777 628.5 0 777 261 404 628.5 0 404 262 340 628.5 0 340 263 95 628.5 0 95 264 21 628.5 0 21 265 31 628.5 0 31 266 42 628.5 0 42 267 38 628.5 0 38 268 35 628.5 0 35 269 29 628.5 0 29 270 19 628.5 0 19 271 22 628.5 0 22 272 24 628.5 0 24 273 43 628.5 0 43 274 29 628.5 0 29 275 28 628.5 0 28 276 47 628.5 0 47 277 31 628.5 0 31 278 30 628.5 0 30 279 158 628.5 0 158 280 245 628.5 0 245 281 366 628.5 0 366 282 39 628.5 0 39 283 29 628.5 0 29 284 42 628.5 0 42 285 29 628.5 0 29 286 41 628.5 0 41 287 30 628.5 0 30 288 26 628.5 0 26 289 32 628.5 0 32 290 22 628.5 0 22 291 38 628.5 0 38 292 42 628.5 0 42 293 65 628.5 0 65 294 58 628.5 0 58 295 61 628.5 0 61 296 62 628.5 0 62 297 58 628.5 0 58 298 32 628.5 0 32 299 33 628.5 0 33 300 49 628.5 0 49 301 42 628.5 0 42 Finished in 12.37 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 261,808 Reads with adapters: 134,509 (51.4%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 134,496 (51.4%) Total basepairs processed: 75,329,830 bp Total written (filtered): 36,611,247 bp (48.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 134509 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 44 0.0 3 0 0 43 0 1 19 1303 0.0 3 0 1275 28 20 130474 0.0 4 128292 2094 70 12 6 21 332 0.0 4 38 288 5 1 22 19 0.0 4 3 1 15 23 88 0.0 4 2 0 0 75 11 25 2 0.0 4 2 33 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 93 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 103 2 0.0 4 2 119 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 18 0.0 4 0 0 0 0 18 127 2168 0.0 4 0 0 0 0 2168 128 3 0.0 4 0 0 0 0 3 130 2 0.0 4 0 0 0 0 2 135 33 0.0 4 0 0 0 0 33 178 1 0.0 4 1 205 2 0.0 4 1 0 0 1 227 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 1 257 1 0.0 4 1 260 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.57 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 134,496 Reads with adapters: 2,453 (1.8%) Reads written (passing filters): 134,496 (100.0%) Total basepairs processed: 36,611,247 bp Total written (filtered): 36,580,920 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2453 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.3% C: 6.3% G: 80.9% T: 2.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 279 2101.5 0 279 4 39 525.4 0 39 5 8 131.3 0 8 6 4 32.8 0 4 7 5 8.2 0 5 8 1 2.1 0 1 9 12 0.5 0 0 12 10 29 0.1 1 2 27 11 1595 0.0 1 14 1581 12 436 0.0 1 7 429 13 8 0.0 1 1 7 15 4 0.0 1 0 4 54 1 0.0 1 1 83 1 0.0 1 1 93 1 0.0 1 1 145 1 0.0 1 1 149 1 0.0 1 1 152 5 0.0 1 0 5 171 11 0.0 1 11 191 1 0.0 1 0 1 210 7 0.0 1 4 3 259 2 0.0 1 1 1 271 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.