This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.90 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 136,074 (5.3%) Reads that were too short: 18,611 (0.7%) Reads written (passing filters): 117,463 (4.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 32,749,967 bp (4.2%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 136074 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 745 628.5 0 745 7 2738 628.5 0 2738 8 56961 628.5 0 56961 9 41139 628.5 0 41139 10 178 628.5 0 178 11 50 628.5 0 50 12 15 628.5 0 15 13 30 628.5 0 30 14 37 628.5 0 37 15 112 628.5 0 112 16 38 628.5 0 38 17 75 628.5 0 75 18 73 628.5 0 73 19 96 628.5 0 96 20 106 628.5 0 106 21 33 628.5 0 33 22 28 628.5 0 28 23 33 628.5 0 33 24 39 628.5 0 39 25 31 628.5 0 31 26 22 628.5 0 22 27 24 628.5 0 24 28 84 628.5 0 84 29 208 628.5 0 208 30 103 628.5 0 103 31 23 628.5 0 23 32 25 628.5 0 25 33 32 628.5 0 32 34 44 628.5 0 44 35 50 628.5 0 50 36 46 628.5 0 46 37 84 628.5 0 84 38 28 628.5 0 28 39 32 628.5 0 32 40 39 628.5 0 39 41 26 628.5 0 26 42 33 628.5 0 33 43 107 628.5 0 107 44 108 628.5 0 108 45 479 628.5 0 479 46 433 628.5 0 433 47 524 628.5 0 524 48 26 628.5 0 26 49 24 628.5 0 24 50 26 628.5 0 26 51 24 628.5 0 24 52 17 628.5 0 17 53 13 628.5 0 13 54 15 628.5 0 15 55 16 628.5 0 16 56 15 628.5 0 15 57 20 628.5 0 20 58 20 628.5 0 20 59 18 628.5 0 18 60 17 628.5 0 17 61 27 628.5 0 27 62 24 628.5 0 24 63 23 628.5 0 23 64 37 628.5 0 37 65 18 628.5 0 18 66 25 628.5 0 25 67 23 628.5 0 23 68 84 628.5 0 84 69 32 628.5 0 32 70 68 628.5 0 68 71 165 628.5 0 165 72 56 628.5 0 56 73 109 628.5 0 109 74 45 628.5 0 45 75 16 628.5 0 16 76 21 628.5 0 21 77 16 628.5 0 16 78 26 628.5 0 26 79 36 628.5 0 36 80 111 628.5 0 111 81 42 628.5 0 42 82 36 628.5 0 36 83 53 628.5 0 53 84 66 628.5 0 66 85 44 628.5 0 44 86 37 628.5 0 37 87 26 628.5 0 26 88 23 628.5 0 23 89 14 628.5 0 14 90 26 628.5 0 26 91 29 628.5 0 29 92 26 628.5 0 26 93 50 628.5 0 50 94 564 628.5 0 564 95 521 628.5 0 521 96 184 628.5 0 184 97 333 628.5 0 333 98 30 628.5 0 30 99 22 628.5 0 22 100 15 628.5 0 15 101 19 628.5 0 19 102 26 628.5 0 26 103 19 628.5 0 19 104 27 628.5 0 27 105 15 628.5 0 15 106 13 628.5 0 13 107 24 628.5 0 24 108 17 628.5 0 17 109 21 628.5 0 21 110 36 628.5 0 36 111 47 628.5 0 47 112 88 628.5 0 88 113 502 628.5 0 502 114 347 628.5 0 347 115 432 628.5 0 432 116 68 628.5 0 68 117 43 628.5 0 43 118 20 628.5 0 20 119 26 628.5 0 26 120 29 628.5 0 29 121 27 628.5 0 27 122 42 628.5 0 42 123 56 628.5 0 56 124 73 628.5 0 73 125 44 628.5 0 44 126 32 628.5 0 32 127 37 628.5 0 37 128 32 628.5 0 32 129 34 628.5 0 34 130 43 628.5 0 43 131 33 628.5 0 33 132 52 628.5 0 52 133 40 628.5 0 40 134 47 628.5 0 47 135 166 628.5 0 166 136 142 628.5 0 142 137 373 628.5 0 373 138 467 628.5 0 467 139 259 628.5 0 259 140 355 628.5 0 355 141 54 628.5 0 54 142 191 628.5 0 191 143 165 628.5 0 165 144 96 628.5 0 96 145 31 628.5 0 31 146 22 628.5 0 22 147 34 628.5 0 34 148 46 628.5 0 46 149 86 628.5 0 86 150 40 628.5 0 40 151 37 628.5 0 37 152 19 628.5 0 19 153 34 628.5 0 34 154 29 628.5 0 29 155 35 628.5 0 35 156 36 628.5 0 36 157 18 628.5 0 18 158 29 628.5 0 29 159 21 628.5 0 21 160 18 628.5 0 18 161 26 628.5 0 26 162 62 628.5 0 62 163 71 628.5 0 71 164 116 628.5 0 116 165 59 628.5 0 59 166 37 628.5 0 37 167 115 628.5 0 115 168 207 628.5 0 207 169 343 628.5 0 343 170 504 628.5 0 504 171 28 628.5 0 28 172 37 628.5 0 37 173 33 628.5 0 33 174 33 628.5 0 33 175 26 628.5 0 26 176 18 628.5 0 18 177 26 628.5 0 26 178 35 628.5 0 35 179 67 628.5 0 67 180 108 628.5 0 108 181 86 628.5 0 86 182 48 628.5 0 48 183 79 628.5 0 79 184 187 628.5 0 187 185 361 628.5 0 361 186 368 628.5 0 368 187 72 628.5 0 72 188 44 628.5 0 44 189 43 628.5 0 43 190 51 628.5 0 51 191 52 628.5 0 52 192 47 628.5 0 47 193 28 628.5 0 28 194 31 628.5 0 31 195 18 628.5 0 18 196 22 628.5 0 22 197 24 628.5 0 24 198 41 628.5 0 41 199 79 628.5 0 79 200 74 628.5 0 74 201 78 628.5 0 78 202 60 628.5 0 60 203 61 628.5 0 61 204 319 628.5 0 319 205 451 628.5 0 451 206 472 628.5 0 472 207 191 628.5 0 191 208 65 628.5 0 65 209 87 628.5 0 87 210 132 628.5 0 132 211 160 628.5 0 160 212 68 628.5 0 68 213 82 628.5 0 82 214 58 628.5 0 58 215 112 628.5 0 112 216 134 628.5 0 134 217 106 628.5 0 106 218 63 628.5 0 63 219 78 628.5 0 78 220 77 628.5 0 77 221 77 628.5 0 77 222 84 628.5 0 84 223 147 628.5 0 147 224 264 628.5 0 264 225 2126 628.5 0 2126 226 1899 628.5 0 1899 227 3500 628.5 0 3500 228 3247 628.5 0 3247 229 74 628.5 0 74 230 51 628.5 0 51 231 40 628.5 0 40 232 45 628.5 0 45 233 36 628.5 0 36 234 77 628.5 0 77 235 126 628.5 0 126 236 144 628.5 0 144 237 85 628.5 0 85 238 82 628.5 0 82 239 47 628.5 0 47 240 66 628.5 0 66 241 59 628.5 0 59 242 40 628.5 0 40 243 45 628.5 0 45 244 41 628.5 0 41 245 46 628.5 0 46 246 42 628.5 0 42 247 53 628.5 0 53 248 78 628.5 0 78 249 38 628.5 0 38 250 37 628.5 0 37 251 26 628.5 0 26 252 25 628.5 0 25 253 51 628.5 0 51 254 42 628.5 0 42 255 38 628.5 0 38 256 37 628.5 0 37 257 45 628.5 0 45 258 60 628.5 0 60 259 63 628.5 0 63 260 69 628.5 0 69 261 70 628.5 0 70 262 38 628.5 0 38 263 45 628.5 0 45 264 98 628.5 0 98 265 107 628.5 0 107 266 111 628.5 0 111 267 57 628.5 0 57 268 34 628.5 0 34 269 66 628.5 0 66 270 57 628.5 0 57 271 37 628.5 0 37 272 34 628.5 0 34 273 50 628.5 0 50 274 61 628.5 0 61 275 121 628.5 0 121 276 112 628.5 0 112 277 26 628.5 0 26 278 30 628.5 0 30 279 38 628.5 0 38 280 68 628.5 0 68 281 53 628.5 0 53 282 55 628.5 0 55 283 34 628.5 0 34 284 31 628.5 0 31 285 36 628.5 0 36 286 27 628.5 0 27 287 42 628.5 0 42 288 33 628.5 0 33 289 43 628.5 0 43 290 46 628.5 0 46 291 42 628.5 0 42 292 42 628.5 0 42 293 78 628.5 0 78 294 66 628.5 0 66 295 75 628.5 0 75 296 113 628.5 0 113 297 198 628.5 0 198 298 90 628.5 0 90 299 93 628.5 0 93 300 55 628.5 0 55 301 171 628.5 0 171 Finished in 5.56 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 117,463 Reads with adapters: 55,961 (47.6%) Reads that were too short: 153 (0.1%) Reads written (passing filters): 55,808 (47.5%) Total basepairs processed: 32,749,967 bp Total written (filtered): 15,374,114 bp (46.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 55961 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 14 0.0 2 0 0 0 14 15 33 0.0 3 0 0 19 14 16 587 0.0 3 0 433 100 54 17 54210 0.0 3 49500 3735 773 202 18 232 0.0 3 24 126 20 62 19 139 0.0 3 0 1 67 71 20 390 0.0 3 0 1 0 389 24 14 0.0 3 0 0 0 14 26 1 0.0 3 0 0 0 1 27 5 0.0 3 0 0 0 5 28 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 36 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 1 1 43 1 0.0 3 0 0 0 1 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 7 0.0 3 0 0 0 7 54 12 0.0 3 0 0 1 11 55 4 0.0 3 0 0 0 4 56 10 0.0 3 0 0 0 10 57 5 0.0 3 0 0 0 5 58 12 0.0 3 0 0 1 11 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 66 3 0.0 3 0 0 0 3 68 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 25 0.0 3 0 0 0 25 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 10 0.0 3 0 0 2 8 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 2 1 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 1 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 1 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 109 5 0.0 3 0 0 0 5 112 1 0.0 3 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 124 3 0.0 3 0 0 0 3 125 8 0.0 3 0 0 0 8 126 13 0.0 3 0 0 2 11 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 2 130 2 0.0 3 0 0 0 2 132 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 1 145 1 0.0 3 0 0 0 1 146 7 0.0 3 0 0 0 7 147 5 0.0 3 0 0 0 5 149 1 0.0 3 0 0 0 1 150 5 0.0 3 1 0 0 4 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 4 0.0 3 0 0 0 4 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 1 163 6 0.0 3 0 0 0 6 164 3 0.0 3 0 0 0 3 167 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 3 0.0 3 0 0 0 3 178 2 0.0 3 0 0 0 2 179 5 0.0 3 0 0 0 5 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 4 0.0 3 0 0 0 4 185 3 0.0 3 0 0 0 3 187 2 0.0 3 0 0 0 2 192 3 0.0 3 0 0 0 3 194 9 0.0 3 0 0 0 9 195 2 0.0 3 0 0 0 2 197 2 0.0 3 0 0 0 2 200 2 0.0 3 0 0 0 2 205 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 4 0.0 3 0 0 0 4 218 3 0.0 3 0 0 0 3 221 2 0.0 3 0 0 0 2 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 1 1 243 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 248 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 5 0.0 3 0 0 0 5 281 12 0.0 3 0 0 0 12 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.95 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 55,808 Reads with adapters: 218 (0.4%) Reads written (passing filters): 55,808 (100.0%) Total basepairs processed: 15,374,114 bp Total written (filtered): 15,373,370 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 218 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.8% C: 14.2% G: 34.9% T: 10.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 147 872.0 0 147 4 62 218.0 0 62 5 7 54.5 0 7 6 1 13.6 0 1 14 1 0.0 1 0 1