This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.76 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 125,919 (4.9%) Reads that were too short: 10,243 (0.4%) Reads written (passing filters): 115,676 (4.5%) Total basepairs processed: 774,927,510 bp Total written (filtered): 32,847,948 bp (4.2%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 125919 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 552 628.5 0 552 7 1374 628.5 0 1374 8 52257 628.5 0 52257 9 51809 628.5 0 51809 10 104 628.5 0 104 11 37 628.5 0 37 12 22 628.5 0 22 13 20 628.5 0 20 14 16 628.5 0 16 15 12 628.5 0 12 16 15 628.5 0 15 17 12 628.5 0 12 18 41 628.5 0 41 19 30 628.5 0 30 20 29 628.5 0 29 21 13 628.5 0 13 22 20 628.5 0 20 23 22 628.5 0 22 24 14 628.5 0 14 25 13 628.5 0 13 26 14 628.5 0 14 27 15 628.5 0 15 28 25 628.5 0 25 29 26 628.5 0 26 30 40 628.5 0 40 31 23 628.5 0 23 32 25 628.5 0 25 33 20 628.5 0 20 34 27 628.5 0 27 35 21 628.5 0 21 36 24 628.5 0 24 37 35 628.5 0 35 38 11 628.5 0 11 39 13 628.5 0 13 40 14 628.5 0 14 41 13 628.5 0 13 42 23 628.5 0 23 43 49 628.5 0 49 44 39 628.5 0 39 45 193 628.5 0 193 46 238 628.5 0 238 47 289 628.5 0 289 48 19 628.5 0 19 49 18 628.5 0 18 50 17 628.5 0 17 51 16 628.5 0 16 52 28 628.5 0 28 53 24 628.5 0 24 54 18 628.5 0 18 55 13 628.5 0 13 56 26 628.5 0 26 57 17 628.5 0 17 58 18 628.5 0 18 59 16 628.5 0 16 60 12 628.5 0 12 61 19 628.5 0 19 62 21 628.5 0 21 63 10 628.5 0 10 64 21 628.5 0 21 65 20 628.5 0 20 66 19 628.5 0 19 67 18 628.5 0 18 68 70 628.5 0 70 69 17 628.5 0 17 70 28 628.5 0 28 71 36 628.5 0 36 72 28 628.5 0 28 73 53 628.5 0 53 74 17 628.5 0 17 75 15 628.5 0 15 76 16 628.5 0 16 77 26 628.5 0 26 78 28 628.5 0 28 79 43 628.5 0 43 80 29 628.5 0 29 81 23 628.5 0 23 82 24 628.5 0 24 83 16 628.5 0 16 84 28 628.5 0 28 85 23 628.5 0 23 86 18 628.5 0 18 87 28 628.5 0 28 88 31 628.5 0 31 89 24 628.5 0 24 90 29 628.5 0 29 91 29 628.5 0 29 92 39 628.5 0 39 93 50 628.5 0 50 94 643 628.5 0 643 95 593 628.5 0 593 96 174 628.5 0 174 97 350 628.5 0 350 98 18 628.5 0 18 99 28 628.5 0 28 100 22 628.5 0 22 101 25 628.5 0 25 102 11 628.5 0 11 103 22 628.5 0 22 104 10 628.5 0 10 105 8 628.5 0 8 106 18 628.5 0 18 107 12 628.5 0 12 108 19 628.5 0 19 109 13 628.5 0 13 110 20 628.5 0 20 111 20 628.5 0 20 112 58 628.5 0 58 113 128 628.5 0 128 114 135 628.5 0 135 115 218 628.5 0 218 116 49 628.5 0 49 117 21 628.5 0 21 118 7 628.5 0 7 119 18 628.5 0 18 120 35 628.5 0 35 121 19 628.5 0 19 122 23 628.5 0 23 123 17 628.5 0 17 124 73 628.5 0 73 125 23 628.5 0 23 126 25 628.5 0 25 127 20 628.5 0 20 128 28 628.5 0 28 129 24 628.5 0 24 130 23 628.5 0 23 131 20 628.5 0 20 132 37 628.5 0 37 133 29 628.5 0 29 134 34 628.5 0 34 135 119 628.5 0 119 136 75 628.5 0 75 137 302 628.5 0 302 138 400 628.5 0 400 139 212 628.5 0 212 140 414 628.5 0 414 141 28 628.5 0 28 142 109 628.5 0 109 143 68 628.5 0 68 144 35 628.5 0 35 145 29 628.5 0 29 146 17 628.5 0 17 147 12 628.5 0 12 148 22 628.5 0 22 149 30 628.5 0 30 150 23 628.5 0 23 151 24 628.5 0 24 152 20 628.5 0 20 153 12 628.5 0 12 154 19 628.5 0 19 155 23 628.5 0 23 156 27 628.5 0 27 157 19 628.5 0 19 158 31 628.5 0 31 159 19 628.5 0 19 160 21 628.5 0 21 161 22 628.5 0 22 162 43 628.5 0 43 163 52 628.5 0 52 164 32 628.5 0 32 165 25 628.5 0 25 166 18 628.5 0 18 167 42 628.5 0 42 168 101 628.5 0 101 169 162 628.5 0 162 170 201 628.5 0 201 171 19 628.5 0 19 172 17 628.5 0 17 173 19 628.5 0 19 174 29 628.5 0 29 175 14 628.5 0 14 176 15 628.5 0 15 177 31 628.5 0 31 178 24 628.5 0 24 179 20 628.5 0 20 180 26 628.5 0 26 181 25 628.5 0 25 182 32 628.5 0 32 183 32 628.5 0 32 184 68 628.5 0 68 185 131 628.5 0 131 186 132 628.5 0 132 187 49 628.5 0 49 188 38 628.5 0 38 189 21 628.5 0 21 190 34 628.5 0 34 191 28 628.5 0 28 192 24 628.5 0 24 193 22 628.5 0 22 194 16 628.5 0 16 195 22 628.5 0 22 196 13 628.5 0 13 197 28 628.5 0 28 198 41 628.5 0 41 199 49 628.5 0 49 200 27 628.5 0 27 201 25 628.5 0 25 202 29 628.5 0 29 203 31 628.5 0 31 204 101 628.5 0 101 205 136 628.5 0 136 206 119 628.5 0 119 207 84 628.5 0 84 208 36 628.5 0 36 209 27 628.5 0 27 210 54 628.5 0 54 211 81 628.5 0 81 212 27 628.5 0 27 213 48 628.5 0 48 214 32 628.5 0 32 215 45 628.5 0 45 216 72 628.5 0 72 217 35 628.5 0 35 218 29 628.5 0 29 219 34 628.5 0 34 220 46 628.5 0 46 221 42 628.5 0 42 222 52 628.5 0 52 223 95 628.5 0 95 224 180 628.5 0 180 225 1166 628.5 0 1166 226 1164 628.5 0 1164 227 2117 628.5 0 2117 228 2103 628.5 0 2103 229 36 628.5 0 36 230 19 628.5 0 19 231 35 628.5 0 35 232 21 628.5 0 21 233 20 628.5 0 20 234 33 628.5 0 33 235 36 628.5 0 36 236 46 628.5 0 46 237 41 628.5 0 41 238 27 628.5 0 27 239 29 628.5 0 29 240 29 628.5 0 29 241 31 628.5 0 31 242 26 628.5 0 26 243 25 628.5 0 25 244 16 628.5 0 16 245 18 628.5 0 18 246 18 628.5 0 18 247 29 628.5 0 29 248 26 628.5 0 26 249 25 628.5 0 25 250 28 628.5 0 28 251 17 628.5 0 17 252 21 628.5 0 21 253 31 628.5 0 31 254 34 628.5 0 34 255 31 628.5 0 31 256 36 628.5 0 36 257 30 628.5 0 30 258 33 628.5 0 33 259 35 628.5 0 35 260 33 628.5 0 33 261 24 628.5 0 24 262 21 628.5 0 21 263 25 628.5 0 25 264 47 628.5 0 47 265 43 628.5 0 43 266 29 628.5 0 29 267 32 628.5 0 32 268 29 628.5 0 29 269 34 628.5 0 34 270 28 628.5 0 28 271 26 628.5 0 26 272 19 628.5 0 19 273 24 628.5 0 24 274 29 628.5 0 29 275 28 628.5 0 28 276 27 628.5 0 27 277 25 628.5 0 25 278 32 628.5 0 32 279 31 628.5 0 31 280 33 628.5 0 33 281 31 628.5 0 31 282 22 628.5 0 22 283 20 628.5 0 20 284 23 628.5 0 23 285 22 628.5 0 22 286 28 628.5 0 28 287 20 628.5 0 20 288 28 628.5 0 28 289 28 628.5 0 28 290 35 628.5 0 35 291 27 628.5 0 27 292 36 628.5 0 36 293 44 628.5 0 44 294 34 628.5 0 34 295 46 628.5 0 46 296 48 628.5 0 48 297 84 628.5 0 84 298 35 628.5 0 35 299 35 628.5 0 35 300 27 628.5 0 27 301 84 628.5 0 84 Finished in 5.98 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 115,676 Reads with adapters: 56,100 (48.5%) Reads that were too short: 25 (0.0%) Reads written (passing filters): 56,075 (48.5%) Total basepairs processed: 32,847,948 bp Total written (filtered): 15,236,887 bp (46.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 56100 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 17 0.0 3 0 0 17 19 466 0.0 3 0 459 7 20 55206 0.0 4 54318 860 21 6 1 21 137 0.0 4 34 101 2 22 13 0.0 4 2 2 9 23 44 0.0 4 0 0 0 41 3 24 3 0.0 4 1 0 0 1 1 25 1 0.0 4 1 29 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 89 19 0.0 4 1 0 0 0 18 109 1 0.0 4 0 0 1 126 1 0.0 4 0 0 0 0 1 127 162 0.0 4 0 0 0 0 162 130 1 0.0 4 0 0 0 0 1 151 2 0.0 4 1 0 1 153 3 0.0 4 1 0 1 0 1 168 1 0.0 4 0 0 0 0 1 178 1 0.0 4 1 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 237 7 0.0 4 0 0 0 0 7 253 1 0.0 4 1 257 1 0.0 4 0 0 0 1 262 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.02 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 56,075 Reads with adapters: 8,784 (15.7%) Reads written (passing filters): 56,075 (100.0%) Total basepairs processed: 15,236,887 bp Total written (filtered): 15,147,264 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8784 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 3.3% G: 94.1% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 876.2 0 14 4 9 219.0 0 9 5 1 54.8 0 1 6 3 13.7 0 3 8 3 0.9 0 3 9 4524 0.2 0 178 4346 10 3124 0.1 1 128 2996 11 1045 0.0 1 7 1038 12 12 0.0 1 0 12 13 6 0.0 1 0 6 19 1 0.0 1 1 23 10 0.0 1 9 1 70 1 0.0 1 1 107 1 0.0 1 1 108 1 0.0 1 1 120 1 0.0 1 1 150 9 0.0 1 0 9 151 1 0.0 1 0 1 169 1 0.0 1 1 189 2 0.0 1 0 2 207 1 0.0 1 1 208 10 0.0 1 6 4 209 4 0.0 1 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.