This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGAAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.27 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 621,513 (24.1%) Reads that were too short: 13,807 (0.5%) Reads written (passing filters): 607,706 (23.6%) Total basepairs processed: 774,927,510 bp Total written (filtered): 129,522,087 bp (16.7%) === Adapter 1 === Sequence: AGAAGA; Type: regular 5'; Length: 6; Trimmed: 621513 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3003 628.5 0 3003 7 125166 628.5 0 125166 8 104424 628.5 0 104424 9 191 628.5 0 191 10 127 628.5 0 127 11 117 628.5 0 117 12 91 628.5 0 91 13 101 628.5 0 101 14 103 628.5 0 103 15 117 628.5 0 117 16 99 628.5 0 99 17 59 628.5 0 59 18 86 628.5 0 86 19 86 628.5 0 86 20 68 628.5 0 68 21 81 628.5 0 81 22 99 628.5 0 99 23 107 628.5 0 107 24 84 628.5 0 84 25 231 628.5 0 231 26 197 628.5 0 197 27 253 628.5 0 253 28 124 628.5 0 124 29 79 628.5 0 79 30 108 628.5 0 108 31 79 628.5 0 79 32 68 628.5 0 68 33 66 628.5 0 66 34 94 628.5 0 94 35 124 628.5 0 124 36 106 628.5 0 106 37 110 628.5 0 110 38 80 628.5 0 80 39 92 628.5 0 92 40 81 628.5 0 81 41 75 628.5 0 75 42 105 628.5 0 105 43 117 628.5 0 117 44 72 628.5 0 72 45 89 628.5 0 89 46 110 628.5 0 110 47 50 628.5 0 50 48 105 628.5 0 105 49 101 628.5 0 101 50 81 628.5 0 81 51 119 628.5 0 119 52 111 628.5 0 111 53 124 628.5 0 124 54 91 628.5 0 91 55 84 628.5 0 84 56 119 628.5 0 119 57 71 628.5 0 71 58 81 628.5 0 81 59 83 628.5 0 83 60 106 628.5 0 106 61 172 628.5 0 172 62 110 628.5 0 110 63 127 628.5 0 127 64 120 628.5 0 120 65 104 628.5 0 104 66 102 628.5 0 102 67 111 628.5 0 111 68 137 628.5 0 137 69 161 628.5 0 161 70 145 628.5 0 145 71 122 628.5 0 122 72 238 628.5 0 238 73 120 628.5 0 120 74 513 628.5 0 513 75 220 628.5 0 220 76 231 628.5 0 231 77 260 628.5 0 260 78 155 628.5 0 155 79 305 628.5 0 305 80 293 628.5 0 293 81 1200 628.5 0 1200 82 859 628.5 0 859 83 1837 628.5 0 1837 84 248 628.5 0 248 85 310 628.5 0 310 86 455 628.5 0 455 87 114 628.5 0 114 88 102 628.5 0 102 89 114 628.5 0 114 90 111 628.5 0 111 91 90 628.5 0 90 92 115 628.5 0 115 93 145 628.5 0 145 94 154 628.5 0 154 95 159 628.5 0 159 96 146 628.5 0 146 97 127 628.5 0 127 98 144 628.5 0 144 99 145 628.5 0 145 100 161 628.5 0 161 101 225 628.5 0 225 102 237 628.5 0 237 103 299 628.5 0 299 104 358 628.5 0 358 105 610 628.5 0 610 106 263 628.5 0 263 107 310 628.5 0 310 108 557 628.5 0 557 109 297 628.5 0 297 110 322 628.5 0 322 111 523 628.5 0 523 112 607 628.5 0 607 113 599 628.5 0 599 114 611 628.5 0 611 115 921 628.5 0 921 116 1082 628.5 0 1082 117 1243 628.5 0 1243 118 13343 628.5 0 13343 119 32457 628.5 0 32457 120 31896 628.5 0 31896 121 1927 628.5 0 1927 122 3215 628.5 0 3215 123 3960 628.5 0 3960 124 471 628.5 0 471 125 287 628.5 0 287 126 359 628.5 0 359 127 460 628.5 0 460 128 535 628.5 0 535 129 349 628.5 0 349 130 465 628.5 0 465 131 753 628.5 0 753 132 1333 628.5 0 1333 133 624 628.5 0 624 134 693 628.5 0 693 135 972 628.5 0 972 136 673 628.5 0 673 137 803 628.5 0 803 138 1226 628.5 0 1226 139 2757 628.5 0 2757 140 9692 628.5 0 9692 141 10434 628.5 0 10434 142 8388 628.5 0 8388 143 2373 628.5 0 2373 144 4510 628.5 0 4510 145 31544 628.5 0 31544 146 49387 628.5 0 49387 147 69675 628.5 0 69675 148 7079 628.5 0 7079 149 4796 628.5 0 4796 150 1213 628.5 0 1213 151 921 628.5 0 921 152 874 628.5 0 874 153 755 628.5 0 755 154 699 628.5 0 699 155 742 628.5 0 742 156 440 628.5 0 440 157 822 628.5 0 822 158 1063 628.5 0 1063 159 1007 628.5 0 1007 160 410 628.5 0 410 161 454 628.5 0 454 162 569 628.5 0 569 163 837 628.5 0 837 164 988 628.5 0 988 165 7560 628.5 0 7560 166 12185 628.5 0 12185 167 14940 628.5 0 14940 168 1141 628.5 0 1141 169 294 628.5 0 294 170 301 628.5 0 301 171 140 628.5 0 140 172 112 628.5 0 112 173 152 628.5 0 152 174 192 628.5 0 192 175 119 628.5 0 119 176 95 628.5 0 95 177 110 628.5 0 110 178 119 628.5 0 119 179 99 628.5 0 99 180 86 628.5 0 86 181 140 628.5 0 140 182 124 628.5 0 124 183 124 628.5 0 124 184 136 628.5 0 136 185 112 628.5 0 112 186 139 628.5 0 139 187 101 628.5 0 101 188 129 628.5 0 129 189 115 628.5 0 115 190 161 628.5 0 161 191 129 628.5 0 129 192 176 628.5 0 176 193 259 628.5 0 259 194 357 628.5 0 357 195 270 628.5 0 270 196 103 628.5 0 103 197 128 628.5 0 128 198 128 628.5 0 128 199 175 628.5 0 175 200 143 628.5 0 143 201 141 628.5 0 141 202 113 628.5 0 113 203 101 628.5 0 101 204 136 628.5 0 136 205 154 628.5 0 154 206 205 628.5 0 205 207 157 628.5 0 157 208 129 628.5 0 129 209 150 628.5 0 150 210 134 628.5 0 134 211 139 628.5 0 139 212 143 628.5 0 143 213 117 628.5 0 117 214 104 628.5 0 104 215 109 628.5 0 109 216 336 628.5 0 336 217 428 628.5 0 428 218 604 628.5 0 604 219 388 628.5 0 388 220 262 628.5 0 262 221 126 628.5 0 126 222 98 628.5 0 98 223 118 628.5 0 118 224 112 628.5 0 112 225 165 628.5 0 165 226 386 628.5 0 386 227 171 628.5 0 171 228 167 628.5 0 167 229 90 628.5 0 90 230 82 628.5 0 82 231 102 628.5 0 102 232 107 628.5 0 107 233 92 628.5 0 92 234 83 628.5 0 83 235 92 628.5 0 92 236 86 628.5 0 86 237 78 628.5 0 78 238 95 628.5 0 95 239 102 628.5 0 102 240 109 628.5 0 109 241 111 628.5 0 111 242 101 628.5 0 101 243 109 628.5 0 109 244 150 628.5 0 150 245 119 628.5 0 119 246 121 628.5 0 121 247 131 628.5 0 131 248 113 628.5 0 113 249 85 628.5 0 85 250 89 628.5 0 89 251 72 628.5 0 72 252 94 628.5 0 94 253 109 628.5 0 109 254 144 628.5 0 144 255 133 628.5 0 133 256 109 628.5 0 109 257 128 628.5 0 128 258 111 628.5 0 111 259 113 628.5 0 113 260 106 628.5 0 106 261 97 628.5 0 97 262 157 628.5 0 157 263 133 628.5 0 133 264 133 628.5 0 133 265 142 628.5 0 142 266 261 628.5 0 261 267 162 628.5 0 162 268 108 628.5 0 108 269 111 628.5 0 111 270 93 628.5 0 93 271 112 628.5 0 112 272 105 628.5 0 105 273 115 628.5 0 115 274 121 628.5 0 121 275 131 628.5 0 131 276 97 628.5 0 97 277 114 628.5 0 114 278 143 628.5 0 143 279 142 628.5 0 142 280 123 628.5 0 123 281 124 628.5 0 124 282 103 628.5 0 103 283 103 628.5 0 103 284 91 628.5 0 91 285 91 628.5 0 91 286 76 628.5 0 76 287 97 628.5 0 97 288 147 628.5 0 147 289 118 628.5 0 118 290 115 628.5 0 115 291 106 628.5 0 106 292 98 628.5 0 98 293 102 628.5 0 102 294 118 628.5 0 118 295 116 628.5 0 116 296 106 628.5 0 106 297 114 628.5 0 114 298 125 628.5 0 125 299 130 628.5 0 130 300 174 628.5 0 174 301 335 628.5 0 335 Finished in 22.12 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 607,706 Reads with adapters: 133,151 (21.9%) Reads that were too short: 1,616 (0.3%) Reads written (passing filters): 131,535 (21.6%) Total basepairs processed: 129,522,087 bp Total written (filtered): 35,207,210 bp (27.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 133151 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 27 0.0 2 0 0 0 27 15 94 0.0 3 0 0 34 60 16 781 0.0 3 0 602 106 73 17 121009 0.0 3 110616 8351 1638 404 18 546 0.0 3 17 203 37 289 19 245 0.0 3 9 8 80 148 20 888 0.0 3 10 4 1 873 21 1 0.0 3 0 0 0 1 23 81 0.0 3 0 0 3 78 24 3 0.0 3 0 0 0 3 25 7 0.0 3 0 0 1 6 26 4482 0.0 3 0 0 64 4418 27 13 0.0 3 2 0 1 10 28 6 0.0 3 1 0 0 5 29 604 0.0 3 1 0 25 578 30 4 0.0 3 0 0 0 4 31 2 0.0 3 1 0 0 1 32 10 0.0 3 0 0 1 9 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 2 0.0 3 1 0 0 1 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 1 0.0 3 1 41 1 0.0 3 0 0 0 1 42 18 0.0 3 0 0 1 17 43 2 0.0 3 0 0 0 2 44 3 0.0 3 0 0 0 3 45 5 0.0 3 0 0 0 5 46 6 0.0 3 0 0 0 6 47 31 0.0 3 0 0 2 29 48 31 0.0 3 0 0 0 31 49 21 0.0 3 0 0 0 21 50 297 0.0 3 0 0 7 290 51 7 0.0 3 0 0 0 7 52 90 0.0 3 0 0 1 89 53 126 0.0 3 0 0 3 123 54 7 0.0 3 0 0 0 7 55 6 0.0 3 0 0 0 6 56 6 0.0 3 0 0 1 5 57 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 60 2 0.0 3 0 0 1 1 61 3 0.0 3 0 0 0 3 63 3 0.0 3 0 0 0 3 64 2 0.0 3 0 0 0 2 65 3 0.0 3 0 0 0 3 66 2 0.0 3 0 0 0 2 68 7 0.0 3 0 0 1 6 69 2 0.0 3 0 0 0 2 70 13 0.0 3 0 0 1 12 72 38 0.0 3 0 0 1 37 73 364 0.0 3 0 0 4 360 74 1362 0.0 3 0 0 5 1357 75 6 0.0 3 0 0 2 4 76 57 0.0 3 0 0 1 56 77 224 0.0 3 0 0 9 215 78 9 0.0 3 0 0 0 9 79 25 0.0 3 0 0 2 23 80 4 0.0 3 0 0 1 3 85 4 0.0 3 0 0 0 4 86 43 0.0 3 0 0 0 43 87 5 0.0 3 0 0 0 5 88 1 0.0 3 0 0 0 1 89 8 0.0 3 0 0 0 8 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 92 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 95 6 0.0 3 0 0 1 5 96 8 0.0 3 0 1 0 7 97 5 0.0 3 0 0 1 4 98 14 0.0 3 0 0 0 14 99 13 0.0 3 0 0 0 13 100 7 0.0 3 0 0 0 7 101 8 0.0 3 0 0 0 8 103 5 0.0 3 0 0 1 4 104 4 0.0 3 0 0 0 4 105 3 0.0 3 0 0 0 3 106 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 108 6 0.0 3 0 0 0 6 110 21 0.0 3 0 0 0 21 111 32 0.0 3 0 0 0 32 112 2 0.0 3 0 0 0 2 113 7 0.0 3 0 0 1 6 114 10 0.0 3 0 0 1 9 115 2 0.0 3 0 0 0 2 116 4 0.0 3 0 0 0 4 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 1 119 4 0.0 3 0 0 1 3 121 3 0.0 3 0 0 0 3 123 3 0.0 3 0 1 0 2 124 14 0.0 3 0 0 2 12 125 24 0.0 3 0 0 1 23 126 16 0.0 3 0 0 0 16 127 73 0.0 3 0 0 4 69 128 113 0.0 3 0 0 7 106 129 44 0.0 3 0 0 0 44 130 2 0.0 3 0 0 0 2 131 3 0.0 3 0 0 0 3 132 2 0.0 3 0 0 0 2 133 36 0.0 3 0 0 0 36 134 15 0.0 3 0 0 0 15 135 12 0.0 3 0 0 0 12 136 3 0.0 3 0 0 0 3 137 8 0.0 3 0 0 0 8 138 180 0.0 3 0 0 2 178 139 19 0.0 3 0 0 0 19 140 20 0.0 3 0 0 0 20 141 92 0.0 3 0 0 5 87 142 7 0.0 3 0 0 0 7 143 4 0.0 3 0 0 0 4 144 2 0.0 3 0 0 0 2 145 2 0.0 3 0 0 0 2 146 54 0.0 3 0 0 1 53 147 41 0.0 3 0 0 0 41 148 10 0.0 3 0 0 0 10 149 6 0.0 3 0 0 0 6 150 4 0.0 3 0 0 0 4 151 14 0.0 3 0 0 0 14 152 4 0.0 3 0 0 1 3 153 3 0.0 3 0 0 0 3 154 3 0.0 3 0 0 0 3 155 10 0.0 3 0 0 0 10 156 106 0.0 3 0 0 5 101 157 4 0.0 3 0 0 0 4 158 6 0.0 3 0 0 0 6 159 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 161 5 0.0 3 0 0 0 5 162 6 0.0 3 0 0 0 6 163 3 0.0 3 0 0 0 3 164 8 0.0 3 0 0 0 8 165 11 0.0 3 0 0 1 10 166 32 0.0 3 0 0 1 31 167 7 0.0 3 0 0 0 7 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 8 0.0 3 0 0 1 7 171 4 0.0 3 0 0 0 4 172 2 0.0 3 0 1 0 1 173 3 0.0 3 0 0 0 3 174 16 0.0 3 0 0 0 16 175 3 0.0 3 0 0 0 3 176 10 0.0 3 0 0 0 10 177 3 0.0 3 0 0 0 3 178 3 0.0 3 0 0 0 3 179 2 0.0 3 0 0 0 2 180 3 0.0 3 0 0 0 3 181 2 0.0 3 0 0 0 2 182 4 0.0 3 0 0 0 4 183 6 0.0 3 0 0 0 6 184 5 0.0 3 0 0 0 5 187 5 0.0 3 0 0 1 4 189 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 2 0.0 3 0 0 0 2 192 14 0.0 3 0 0 0 14 193 3 0.0 3 0 0 0 3 194 18 0.0 3 0 0 1 17 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 198 2 0.0 3 0 0 0 2 204 3 0.0 3 0 0 1 2 205 5 0.0 3 0 0 0 5 206 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 212 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 217 18 0.0 3 0 0 0 18 218 2 0.0 3 0 0 0 2 219 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 237 2 0.0 3 0 0 0 2 238 2 0.0 3 0 0 1 1 239 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 259 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 271 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 276 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 279 11 0.0 3 0 0 0 11 280 8 0.0 3 0 0 0 8 281 21 0.0 3 0 0 0 21 282 16 0.0 3 0 0 1 15 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 293 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.51 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 131,535 Reads with adapters: 267 (0.2%) Reads written (passing filters): 131,535 (100.0%) Total basepairs processed: 35,207,210 bp Total written (filtered): 35,206,312 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 267 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.5% C: 21.0% G: 44.9% T: 11.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 215 2055.2 0 215 4 30 513.8 0 30 5 11 128.5 0 11 6 9 32.1 0 9 12 2 0.0 1 0 2