This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.17 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 271,135 (10.5%) Reads that were too short: 11,217 (0.4%) Reads written (passing filters): 259,918 (10.1%) Total basepairs processed: 774,927,510 bp Total written (filtered): 73,007,548 bp (9.4%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 271135 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3444 628.5 0 3444 7 7094 628.5 0 7094 8 119685 628.5 0 119685 9 103498 628.5 0 103498 10 167 628.5 0 167 11 12 628.5 0 12 12 30 628.5 0 30 13 21 628.5 0 21 14 16 628.5 0 16 15 13 628.5 0 13 16 20 628.5 0 20 17 40 628.5 0 40 18 21 628.5 0 21 19 29 628.5 0 29 20 22 628.5 0 22 21 10 628.5 0 10 22 17 628.5 0 17 23 17 628.5 0 17 24 14 628.5 0 14 25 19 628.5 0 19 26 16 628.5 0 16 27 16 628.5 0 16 28 23 628.5 0 23 29 14 628.5 0 14 30 28 628.5 0 28 31 18 628.5 0 18 32 22 628.5 0 22 33 18 628.5 0 18 34 21 628.5 0 21 35 24 628.5 0 24 36 14 628.5 0 14 37 16 628.5 0 16 38 14 628.5 0 14 39 19 628.5 0 19 40 36 628.5 0 36 41 18 628.5 0 18 42 34 628.5 0 34 43 25 628.5 0 25 44 29 628.5 0 29 45 35 628.5 0 35 46 32 628.5 0 32 47 89 628.5 0 89 48 49 628.5 0 49 49 165 628.5 0 165 50 69 628.5 0 69 51 11 628.5 0 11 52 26 628.5 0 26 53 26 628.5 0 26 54 21 628.5 0 21 55 22 628.5 0 22 56 22 628.5 0 22 57 12 628.5 0 12 58 58 628.5 0 58 59 70 628.5 0 70 60 58 628.5 0 58 61 26 628.5 0 26 62 33 628.5 0 33 63 20 628.5 0 20 64 23 628.5 0 23 65 12 628.5 0 12 66 165 628.5 0 165 67 133 628.5 0 133 68 246 628.5 0 246 69 23 628.5 0 23 70 42 628.5 0 42 71 48 628.5 0 48 72 19 628.5 0 19 73 30 628.5 0 30 74 19 628.5 0 19 75 24 628.5 0 24 76 33 628.5 0 33 77 32 628.5 0 32 78 26 628.5 0 26 79 24 628.5 0 24 80 18 628.5 0 18 81 28 628.5 0 28 82 50 628.5 0 50 83 39 628.5 0 39 84 62 628.5 0 62 85 86 628.5 0 86 86 46 628.5 0 46 87 33 628.5 0 33 88 20 628.5 0 20 89 57 628.5 0 57 90 26 628.5 0 26 91 57 628.5 0 57 92 47 628.5 0 47 93 60 628.5 0 60 94 41 628.5 0 41 95 35 628.5 0 35 96 23 628.5 0 23 97 33 628.5 0 33 98 30 628.5 0 30 99 37 628.5 0 37 100 43 628.5 0 43 101 27 628.5 0 27 102 33 628.5 0 33 103 63 628.5 0 63 104 93 628.5 0 93 105 35 628.5 0 35 106 46 628.5 0 46 107 68 628.5 0 68 108 48 628.5 0 48 109 56 628.5 0 56 110 65 628.5 0 65 111 242 628.5 0 242 112 551 628.5 0 551 113 231 628.5 0 231 114 156 628.5 0 156 115 824 628.5 0 824 116 505 628.5 0 505 117 2282 628.5 0 2282 118 2527 628.5 0 2527 119 4996 628.5 0 4996 120 72 628.5 0 72 121 45 628.5 0 45 122 43 628.5 0 43 123 22 628.5 0 22 124 47 628.5 0 47 125 65 628.5 0 65 126 39 628.5 0 39 127 63 628.5 0 63 128 55 628.5 0 55 129 55 628.5 0 55 130 54 628.5 0 54 131 54 628.5 0 54 132 105 628.5 0 105 133 105 628.5 0 105 134 41 628.5 0 41 135 48 628.5 0 48 136 30 628.5 0 30 137 38 628.5 0 38 138 46 628.5 0 46 139 44 628.5 0 44 140 50 628.5 0 50 141 48 628.5 0 48 142 38 628.5 0 38 143 50 628.5 0 50 144 81 628.5 0 81 145 70 628.5 0 70 146 149 628.5 0 149 147 114 628.5 0 114 148 143 628.5 0 143 149 105 628.5 0 105 150 97 628.5 0 97 151 856 628.5 0 856 152 796 628.5 0 796 153 1099 628.5 0 1099 154 1116 628.5 0 1116 155 39 628.5 0 39 156 67 628.5 0 67 157 50 628.5 0 50 158 27 628.5 0 27 159 44 628.5 0 44 160 74 628.5 0 74 161 61 628.5 0 61 162 223 628.5 0 223 163 391 628.5 0 391 164 700 628.5 0 700 165 35 628.5 0 35 166 53 628.5 0 53 167 32 628.5 0 32 168 32 628.5 0 32 169 48 628.5 0 48 170 26 628.5 0 26 171 39 628.5 0 39 172 45 628.5 0 45 173 41 628.5 0 41 174 31 628.5 0 31 175 36 628.5 0 36 176 36 628.5 0 36 177 28 628.5 0 28 178 48 628.5 0 48 179 259 628.5 0 259 180 130 628.5 0 130 181 206 628.5 0 206 182 111 628.5 0 111 183 225 628.5 0 225 184 55 628.5 0 55 185 79 628.5 0 79 186 22 628.5 0 22 187 42 628.5 0 42 188 32 628.5 0 32 189 51 628.5 0 51 190 55 628.5 0 55 191 69 628.5 0 69 192 69 628.5 0 69 193 47 628.5 0 47 194 28 628.5 0 28 195 32 628.5 0 32 196 33 628.5 0 33 197 45 628.5 0 45 198 55 628.5 0 55 199 46 628.5 0 46 200 42 628.5 0 42 201 40 628.5 0 40 202 35 628.5 0 35 203 53 628.5 0 53 204 47 628.5 0 47 205 47 628.5 0 47 206 40 628.5 0 40 207 39 628.5 0 39 208 46 628.5 0 46 209 35 628.5 0 35 210 53 628.5 0 53 211 58 628.5 0 58 212 57 628.5 0 57 213 55 628.5 0 55 214 85 628.5 0 85 215 40 628.5 0 40 216 66 628.5 0 66 217 130 628.5 0 130 218 253 628.5 0 253 219 236 628.5 0 236 220 247 628.5 0 247 221 88 628.5 0 88 222 34 628.5 0 34 223 57 628.5 0 57 224 66 628.5 0 66 225 80 628.5 0 80 226 74 628.5 0 74 227 86 628.5 0 86 228 91 628.5 0 91 229 94 628.5 0 94 230 108 628.5 0 108 231 286 628.5 0 286 232 234 628.5 0 234 233 489 628.5 0 489 234 209 628.5 0 209 235 182 628.5 0 182 236 224 628.5 0 224 237 179 628.5 0 179 238 122 628.5 0 122 239 131 628.5 0 131 240 96 628.5 0 96 241 44 628.5 0 44 242 60 628.5 0 60 243 61 628.5 0 61 244 89 628.5 0 89 245 160 628.5 0 160 246 157 628.5 0 157 247 276 628.5 0 276 248 118 628.5 0 118 249 98 628.5 0 98 250 81 628.5 0 81 251 96 628.5 0 96 252 132 628.5 0 132 253 128 628.5 0 128 254 184 628.5 0 184 255 133 628.5 0 133 256 111 628.5 0 111 257 101 628.5 0 101 258 66 628.5 0 66 259 62 628.5 0 62 260 48 628.5 0 48 261 48 628.5 0 48 262 36 628.5 0 36 263 71 628.5 0 71 264 79 628.5 0 79 265 111 628.5 0 111 266 101 628.5 0 101 267 107 628.5 0 107 268 92 628.5 0 92 269 45 628.5 0 45 270 56 628.5 0 56 271 78 628.5 0 78 272 126 628.5 0 126 273 114 628.5 0 114 274 55 628.5 0 55 275 57 628.5 0 57 276 45 628.5 0 45 277 70 628.5 0 70 278 124 628.5 0 124 279 118 628.5 0 118 280 114 628.5 0 114 281 60 628.5 0 60 282 120 628.5 0 120 283 162 628.5 0 162 284 249 628.5 0 249 285 175 628.5 0 175 286 100 628.5 0 100 287 95 628.5 0 95 288 102 628.5 0 102 289 82 628.5 0 82 290 74 628.5 0 74 291 151 628.5 0 151 292 130 628.5 0 130 293 182 628.5 0 182 294 104 628.5 0 104 295 96 628.5 0 96 296 81 628.5 0 81 297 124 628.5 0 124 298 141 628.5 0 141 299 253 628.5 0 253 300 163 628.5 0 163 301 169 628.5 0 169 Finished in 11.63 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 259,918 Reads with adapters: 122,301 (47.1%) Reads that were too short: 352 (0.1%) Reads written (passing filters): 121,949 (46.9%) Total basepairs processed: 73,007,548 bp Total written (filtered): 33,590,776 bp (46.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 122301 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 37 0.0 2 0 0 0 37 15 96 0.0 3 0 0 54 42 16 1165 0.0 3 0 830 217 118 17 115283 0.0 3 105191 7987 1688 417 18 3923 0.0 3 3198 514 117 94 19 292 0.0 3 1 6 161 124 20 670 0.0 3 3 0 6 661 21 15 0.0 3 0 0 0 15 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 0 1 24 2 0.0 3 1 0 0 1 27 5 0.0 3 0 1 0 4 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 30 4 0.0 3 0 0 0 4 31 2 0.0 3 1 0 0 1 33 4 0.0 3 1 0 0 3 34 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 2 0.0 3 1 0 0 1 40 48 0.0 3 1 0 0 47 42 3 0.0 3 0 0 0 3 43 14 0.0 3 0 0 0 14 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 51 2 0.0 3 0 0 0 2 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 54 172 0.0 3 0 0 1 171 55 1 0.0 3 0 0 0 1 56 20 0.0 3 0 0 0 20 57 23 0.0 3 0 0 0 23 58 39 0.0 3 0 0 0 39 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 3 0.0 3 0 0 0 3 62 4 0.0 3 0 0 0 4 63 3 0.0 3 0 0 0 3 64 8 0.0 3 0 0 0 8 66 3 0.0 3 0 0 0 3 67 6 0.0 3 0 0 0 6 70 2 0.0 3 0 0 1 1 71 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 5 0.0 3 0 0 0 5 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 1 2 91 11 0.0 3 0 0 0 11 93 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 1 105 12 0.0 3 0 0 0 12 106 4 0.0 3 0 0 0 4 107 4 0.0 3 0 0 1 3 108 5 0.0 3 0 0 0 5 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 6 0.0 3 0 1 0 5 116 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 127 3 0.0 3 0 0 0 3 129 3 0.0 3 1 0 0 2 130 2 0.0 3 0 0 0 2 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 6 0.0 3 0 0 0 6 142 5 0.0 3 0 0 0 5 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 155 2 0.0 3 0 0 0 2 156 5 0.0 3 0 0 0 5 158 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 3 0.0 3 0 0 0 3 164 8 0.0 3 0 0 0 8 165 1 0.0 3 0 0 0 1 166 24 0.0 3 0 0 1 23 167 7 0.0 3 0 0 2 5 168 4 0.0 3 0 0 0 4 169 26 0.0 3 0 0 3 23 170 5 0.0 3 0 0 0 5 171 6 0.0 3 0 0 0 6 172 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 7 0.0 3 0 0 0 7 184 3 0.0 3 0 0 0 3 187 2 0.0 3 0 0 0 2 188 1 0.0 3 0 0 1 190 2 0.0 3 0 0 0 2 192 12 0.0 3 0 0 0 12 193 1 0.0 3 0 0 0 1 194 20 0.0 3 0 0 2 18 195 3 0.0 3 0 0 0 3 196 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 3 0.0 3 0 0 0 3 205 12 0.0 3 0 0 0 12 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 214 1 0.0 3 0 0 1 216 3 0.0 3 0 0 0 3 217 30 0.0 3 0 0 3 27 220 1 0.0 3 0 0 0 1 221 5 0.0 3 0 0 0 5 222 4 0.0 3 0 0 0 4 224 1 0.0 3 0 0 0 1 235 2 0.0 3 0 0 0 2 237 3 0.0 3 0 0 0 3 238 2 0.0 3 0 0 0 2 239 3 0.0 3 0 0 0 3 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 263 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 270 4 0.0 3 0 0 0 4 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 14 0.0 3 0 0 0 14 280 13 0.0 3 0 0 0 13 281 21 0.0 3 0 0 0 21 282 8 0.0 3 0 0 1 7 283 4 0.0 3 0 0 0 4 285 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 290 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.31 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 121,949 Reads with adapters: 438 (0.4%) Reads written (passing filters): 121,949 (100.0%) Total basepairs processed: 33,590,776 bp Total written (filtered): 33,589,223 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 438 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.4% C: 10.7% G: 45.4% T: 8.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 272 1905.5 0 272 4 142 476.4 0 142 5 10 119.1 0 10 6 6 29.8 0 6 7 1 7.4 0 1 10 1 0.1 1 0 1 11 6 0.0 1 0 6