This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.44 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,574,510 Reads with adapters: 271,311 (10.5%) Reads that were too short: 3,723 (0.1%) Reads written (passing filters): 267,588 (10.4%) Total basepairs processed: 774,927,510 bp Total written (filtered): 76,319,943 bp (9.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 271311 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3327 628.5 0 3327 7 4785 628.5 0 4785 8 117908 628.5 0 117908 9 123673 628.5 0 123673 10 137 628.5 0 137 11 28 628.5 0 28 12 42 628.5 0 42 13 19 628.5 0 19 14 18 628.5 0 18 15 15 628.5 0 15 16 33 628.5 0 33 17 16 628.5 0 16 18 25 628.5 0 25 19 23 628.5 0 23 20 18 628.5 0 18 21 15 628.5 0 15 22 29 628.5 0 29 23 21 628.5 0 21 24 8 628.5 0 8 25 17 628.5 0 17 26 16 628.5 0 16 27 16 628.5 0 16 28 16 628.5 0 16 29 18 628.5 0 18 30 26 628.5 0 26 31 10 628.5 0 10 32 16 628.5 0 16 33 13 628.5 0 13 34 25 628.5 0 25 35 24 628.5 0 24 36 20 628.5 0 20 37 25 628.5 0 25 38 18 628.5 0 18 39 15 628.5 0 15 40 21 628.5 0 21 41 18 628.5 0 18 42 14 628.5 0 14 43 17 628.5 0 17 44 14 628.5 0 14 45 24 628.5 0 24 46 20 628.5 0 20 47 34 628.5 0 34 48 24 628.5 0 24 49 34 628.5 0 34 50 23 628.5 0 23 51 22 628.5 0 22 52 24 628.5 0 24 53 37 628.5 0 37 54 24 628.5 0 24 55 30 628.5 0 30 56 29 628.5 0 29 57 17 628.5 0 17 58 18 628.5 0 18 59 36 628.5 0 36 60 29 628.5 0 29 61 22 628.5 0 22 62 30 628.5 0 30 63 23 628.5 0 23 64 19 628.5 0 19 65 13 628.5 0 13 66 53 628.5 0 53 67 46 628.5 0 46 68 80 628.5 0 80 69 11 628.5 0 11 70 33 628.5 0 33 71 16 628.5 0 16 72 24 628.5 0 24 73 23 628.5 0 23 74 18 628.5 0 18 75 25 628.5 0 25 76 31 628.5 0 31 77 32 628.5 0 32 78 24 628.5 0 24 79 37 628.5 0 37 80 29 628.5 0 29 81 21 628.5 0 21 82 34 628.5 0 34 83 36 628.5 0 36 84 25 628.5 0 25 85 38 628.5 0 38 86 30 628.5 0 30 87 19 628.5 0 19 88 38 628.5 0 38 89 35 628.5 0 35 90 28 628.5 0 28 91 36 628.5 0 36 92 36 628.5 0 36 93 39 628.5 0 39 94 29 628.5 0 29 95 24 628.5 0 24 96 31 628.5 0 31 97 32 628.5 0 32 98 36 628.5 0 36 99 25 628.5 0 25 100 28 628.5 0 28 101 40 628.5 0 40 102 36 628.5 0 36 103 50 628.5 0 50 104 111 628.5 0 111 105 33 628.5 0 33 106 45 628.5 0 45 107 75 628.5 0 75 108 43 628.5 0 43 109 43 628.5 0 43 110 63 628.5 0 63 111 246 628.5 0 246 112 567 628.5 0 567 113 177 628.5 0 177 114 75 628.5 0 75 115 197 628.5 0 197 116 182 628.5 0 182 117 1435 628.5 0 1435 118 2616 628.5 0 2616 119 5636 628.5 0 5636 120 35 628.5 0 35 121 21 628.5 0 21 122 32 628.5 0 32 123 21 628.5 0 21 124 22 628.5 0 22 125 22 628.5 0 22 126 27 628.5 0 27 127 41 628.5 0 41 128 35 628.5 0 35 129 22 628.5 0 22 130 32 628.5 0 32 131 33 628.5 0 33 132 40 628.5 0 40 133 30 628.5 0 30 134 16 628.5 0 16 135 27 628.5 0 27 136 28 628.5 0 28 137 27 628.5 0 27 138 21 628.5 0 21 139 23 628.5 0 23 140 18 628.5 0 18 141 27 628.5 0 27 142 21 628.5 0 21 143 19 628.5 0 19 144 31 628.5 0 31 145 28 628.5 0 28 146 42 628.5 0 42 147 35 628.5 0 35 148 46 628.5 0 46 149 35 628.5 0 35 150 27 628.5 0 27 151 257 628.5 0 257 152 200 628.5 0 200 153 353 628.5 0 353 154 254 628.5 0 254 155 30 628.5 0 30 156 36 628.5 0 36 157 23 628.5 0 23 158 10 628.5 0 10 159 24 628.5 0 24 160 22 628.5 0 22 161 33 628.5 0 33 162 69 628.5 0 69 163 69 628.5 0 69 164 97 628.5 0 97 165 28 628.5 0 28 166 26 628.5 0 26 167 8 628.5 0 8 168 21 628.5 0 21 169 24 628.5 0 24 170 10 628.5 0 10 171 31 628.5 0 31 172 20 628.5 0 20 173 29 628.5 0 29 174 13 628.5 0 13 175 12 628.5 0 12 176 28 628.5 0 28 177 12 628.5 0 12 178 30 628.5 0 30 179 153 628.5 0 153 180 79 628.5 0 79 181 105 628.5 0 105 182 80 628.5 0 80 183 299 628.5 0 299 184 25 628.5 0 25 185 74 628.5 0 74 186 12 628.5 0 12 187 30 628.5 0 30 188 7 628.5 0 7 189 17 628.5 0 17 190 24 628.5 0 24 191 30 628.5 0 30 192 27 628.5 0 27 193 27 628.5 0 27 194 16 628.5 0 16 195 16 628.5 0 16 196 20 628.5 0 20 197 19 628.5 0 19 198 11 628.5 0 11 199 17 628.5 0 17 200 23 628.5 0 23 201 21 628.5 0 21 202 19 628.5 0 19 203 21 628.5 0 21 204 16 628.5 0 16 205 13 628.5 0 13 206 18 628.5 0 18 207 13 628.5 0 13 208 15 628.5 0 15 209 12 628.5 0 12 210 34 628.5 0 34 211 24 628.5 0 24 212 23 628.5 0 23 213 28 628.5 0 28 214 39 628.5 0 39 215 18 628.5 0 18 216 34 628.5 0 34 217 27 628.5 0 27 218 111 628.5 0 111 219 140 628.5 0 140 220 245 628.5 0 245 221 56 628.5 0 56 222 16 628.5 0 16 223 26 628.5 0 26 224 25 628.5 0 25 225 32 628.5 0 32 226 21 628.5 0 21 227 23 628.5 0 23 228 29 628.5 0 29 229 29 628.5 0 29 230 42 628.5 0 42 231 61 628.5 0 61 232 65 628.5 0 65 233 80 628.5 0 80 234 35 628.5 0 35 235 60 628.5 0 60 236 60 628.5 0 60 237 74 628.5 0 74 238 26 628.5 0 26 239 24 628.5 0 24 240 25 628.5 0 25 241 15 628.5 0 15 242 19 628.5 0 19 243 23 628.5 0 23 244 22 628.5 0 22 245 34 628.5 0 34 246 36 628.5 0 36 247 41 628.5 0 41 248 40 628.5 0 40 249 32 628.5 0 32 250 32 628.5 0 32 251 28 628.5 0 28 252 35 628.5 0 35 253 40 628.5 0 40 254 51 628.5 0 51 255 27 628.5 0 27 256 20 628.5 0 20 257 19 628.5 0 19 258 20 628.5 0 20 259 30 628.5 0 30 260 18 628.5 0 18 261 11 628.5 0 11 262 6 628.5 0 6 263 21 628.5 0 21 264 25 628.5 0 25 265 23 628.5 0 23 266 21 628.5 0 21 267 32 628.5 0 32 268 27 628.5 0 27 269 26 628.5 0 26 270 24 628.5 0 24 271 47 628.5 0 47 272 36 628.5 0 36 273 24 628.5 0 24 274 28 628.5 0 28 275 26 628.5 0 26 276 24 628.5 0 24 277 21 628.5 0 21 278 70 628.5 0 70 279 54 628.5 0 54 280 69 628.5 0 69 281 22 628.5 0 22 282 29 628.5 0 29 283 30 628.5 0 30 284 37 628.5 0 37 285 47 628.5 0 47 286 29 628.5 0 29 287 39 628.5 0 39 288 34 628.5 0 34 289 31 628.5 0 31 290 28 628.5 0 28 291 46 628.5 0 46 292 31 628.5 0 31 293 41 628.5 0 41 294 33 628.5 0 33 295 37 628.5 0 37 296 35 628.5 0 35 297 59 628.5 0 59 298 48 628.5 0 48 299 96 628.5 0 96 300 61 628.5 0 61 301 54 628.5 0 54 Finished in 12.98 s (49 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 267,588 Reads with adapters: 132,097 (49.4%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 132,048 (49.3%) Total basepairs processed: 76,319,943 bp Total written (filtered): 35,888,419 bp (47.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 132097 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 52 0.0 3 0 0 52 19 1205 0.0 3 0 1181 19 1 4 20 127611 0.0 4 125445 2089 71 5 1 21 2650 0.0 4 2336 300 11 3 22 18 0.0 4 0 6 11 0 1 23 127 0.0 4 4 0 1 110 12 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 1 27 1 0.0 4 0 0 1 29 1 0.0 4 1 31 2 0.0 4 2 32 1 0.0 4 0 0 1 40 1 0.0 4 0 1 43 1 0.0 4 1 121 1 0.0 4 1 124 1 0.0 4 1 126 1 0.0 4 0 0 0 0 1 127 194 0.0 4 0 0 0 0 194 128 170 0.0 4 0 0 0 0 170 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 1 173 1 0.0 4 1 175 1 0.0 4 1 193 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 1 215 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 8 0.0 4 0 0 0 0 8 229 4 0.0 4 0 0 0 1 3 230 1 0.0 4 0 0 0 1 236 2 0.0 4 0 1 0 0 1 237 8 0.0 4 0 0 0 0 8 259 1 0.0 4 0 0 0 0 1 260 3 0.0 4 0 0 0 0 3 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 3 0.0 4 0 0 0 0 3 273 1 0.0 4 0 0 0 0 1 283 1 0.0 4 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.71 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 132,048 Reads with adapters: 20,184 (15.3%) Reads written (passing filters): 132,048 (100.0%) Total basepairs processed: 35,888,419 bp Total written (filtered): 35,674,818 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20184 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.1% G: 94.5% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 54 2063.2 0 54 4 36 515.8 0 36 5 17 129.0 0 17 6 6 32.2 0 6 7 5 8.1 0 5 8 5 2.0 0 5 9 9971 0.5 0 378 9593 10 7304 0.1 1 294 7010 11 2645 0.0 1 26 2619 12 23 0.0 1 0 23 13 9 0.0 1 0 9 18 1 0.0 1 0 1 19 4 0.0 1 3 1 21 1 0.0 1 1 23 1 0.0 1 0 1 37 1 0.0 1 1 39 1 0.0 1 0 1 43 1 0.0 1 1 61 1 0.0 1 1 67 1 0.0 1 1 94 1 0.0 1 1 122 1 0.0 1 1 150 5 0.0 1 0 5 151 1 0.0 1 0 1 172 1 0.0 1 1 188 1 0.0 1 1 189 3 0.0 1 0 3 190 1 0.0 1 0 1 195 1 0.0 1 1 208 33 0.0 1 20 13 209 2 0.0 1 1 1 210 1 0.0 1 0 1 223 33 0.0 1 33 224 1 0.0 1 1 227 1 0.0 1 1 228 1 0.0 1 1 235 5 0.0 1 4 1 257 1 0.0 1 1 259 1 0.0 1 1 262 2 0.0 1 2 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.