This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGCGCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.28 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 519,679 (18.2%) Reads that were too short: 45,437 (1.6%) Reads written (passing filters): 474,242 (16.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 121,482,067 bp (14.1%) === Adapter 1 === Sequence: GGCGCA; Type: regular 5'; Length: 6; Trimmed: 519679 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1269 696.4 0 1269 7 32117 696.4 0 32117 8 26240 696.4 0 26240 9 275 696.4 0 275 10 355 696.4 0 355 11 169 696.4 0 169 12 206 696.4 0 206 13 508 696.4 0 508 14 477 696.4 0 477 15 525 696.4 0 525 16 331 696.4 0 331 17 731 696.4 0 731 18 878 696.4 0 878 19 2009 696.4 0 2009 20 30401 696.4 0 30401 21 20022 696.4 0 20022 22 28832 696.4 0 28832 23 330 696.4 0 330 24 214 696.4 0 214 25 324 696.4 0 324 26 149 696.4 0 149 27 120 696.4 0 120 28 80 696.4 0 80 29 160 696.4 0 160 30 107 696.4 0 107 31 208 696.4 0 208 32 376 696.4 0 376 33 452 696.4 0 452 34 519 696.4 0 519 35 1062 696.4 0 1062 36 2307 696.4 0 2307 37 609 696.4 0 609 38 854 696.4 0 854 39 1889 696.4 0 1889 40 685 696.4 0 685 41 680 696.4 0 680 42 1405 696.4 0 1405 43 1352 696.4 0 1352 44 1706 696.4 0 1706 45 1160 696.4 0 1160 46 2220 696.4 0 2220 47 2873 696.4 0 2873 48 6190 696.4 0 6190 49 91327 696.4 0 91327 50 67381 696.4 0 67381 51 115017 696.4 0 115017 52 505 696.4 0 505 53 51 696.4 0 51 54 65 696.4 0 65 55 49 696.4 0 49 56 69 696.4 0 69 57 60 696.4 0 60 58 83 696.4 0 83 59 60 696.4 0 60 60 107 696.4 0 107 61 71 696.4 0 71 62 191 696.4 0 191 63 1051 696.4 0 1051 64 1020 696.4 0 1020 65 1277 696.4 0 1277 66 28 696.4 0 28 67 43 696.4 0 43 68 35 696.4 0 35 69 36 696.4 0 36 70 34 696.4 0 34 71 44 696.4 0 44 72 43 696.4 0 43 73 32 696.4 0 32 74 41 696.4 0 41 75 48 696.4 0 48 76 34 696.4 0 34 77 27 696.4 0 27 78 33 696.4 0 33 79 33 696.4 0 33 80 46 696.4 0 46 81 30 696.4 0 30 82 29 696.4 0 29 83 28 696.4 0 28 84 61 696.4 0 61 85 32 696.4 0 32 86 51 696.4 0 51 87 57 696.4 0 57 88 141 696.4 0 141 89 50 696.4 0 50 90 72 696.4 0 72 91 116 696.4 0 116 92 49 696.4 0 49 93 42 696.4 0 42 94 45 696.4 0 45 95 31 696.4 0 31 96 36 696.4 0 36 97 29 696.4 0 29 98 44 696.4 0 44 99 26 696.4 0 26 100 23 696.4 0 23 101 33 696.4 0 33 102 31 696.4 0 31 103 20 696.4 0 20 104 36 696.4 0 36 105 31 696.4 0 31 106 42 696.4 0 42 107 35 696.4 0 35 108 20 696.4 0 20 109 23 696.4 0 23 110 33 696.4 0 33 111 41 696.4 0 41 112 29 696.4 0 29 113 45 696.4 0 45 114 26 696.4 0 26 115 34 696.4 0 34 116 45 696.4 0 45 117 309 696.4 0 309 118 384 696.4 0 384 119 474 696.4 0 474 120 72 696.4 0 72 121 72 696.4 0 72 122 23 696.4 0 23 123 36 696.4 0 36 124 99 696.4 0 99 125 79 696.4 0 79 126 137 696.4 0 137 127 44 696.4 0 44 128 64 696.4 0 64 129 34 696.4 0 34 130 53 696.4 0 53 131 39 696.4 0 39 132 41 696.4 0 41 133 33 696.4 0 33 134 36 696.4 0 36 135 47 696.4 0 47 136 28 696.4 0 28 137 30 696.4 0 30 138 42 696.4 0 42 139 43 696.4 0 43 140 49 696.4 0 49 141 40 696.4 0 40 142 46 696.4 0 46 143 71 696.4 0 71 144 101 696.4 0 101 145 109 696.4 0 109 146 72 696.4 0 72 147 50 696.4 0 50 148 41 696.4 0 41 149 42 696.4 0 42 150 42 696.4 0 42 151 53 696.4 0 53 152 57 696.4 0 57 153 66 696.4 0 66 154 53 696.4 0 53 155 63 696.4 0 63 156 65 696.4 0 65 157 134 696.4 0 134 158 125 696.4 0 125 159 223 696.4 0 223 160 53 696.4 0 53 161 43 696.4 0 43 162 38 696.4 0 38 163 43 696.4 0 43 164 33 696.4 0 33 165 36 696.4 0 36 166 32 696.4 0 32 167 52 696.4 0 52 168 37 696.4 0 37 169 48 696.4 0 48 170 68 696.4 0 68 171 58 696.4 0 58 172 55 696.4 0 55 173 60 696.4 0 60 174 43 696.4 0 43 175 45 696.4 0 45 176 83 696.4 0 83 177 121 696.4 0 121 178 131 696.4 0 131 179 172 696.4 0 172 180 188 696.4 0 188 181 97 696.4 0 97 182 166 696.4 0 166 183 271 696.4 0 271 184 469 696.4 0 469 185 84 696.4 0 84 186 104 696.4 0 104 187 116 696.4 0 116 188 175 696.4 0 175 189 175 696.4 0 175 190 160 696.4 0 160 191 168 696.4 0 168 192 282 696.4 0 282 193 2238 696.4 0 2238 194 2222 696.4 0 2222 195 6607 696.4 0 6607 196 1381 696.4 0 1381 197 170 696.4 0 170 198 62 696.4 0 62 199 121 696.4 0 121 200 90 696.4 0 90 201 91 696.4 0 91 202 56 696.4 0 56 203 64 696.4 0 64 204 41 696.4 0 41 205 65 696.4 0 65 206 110 696.4 0 110 207 57 696.4 0 57 208 91 696.4 0 91 209 94 696.4 0 94 210 68 696.4 0 68 211 52 696.4 0 52 212 84 696.4 0 84 213 328 696.4 0 328 214 734 696.4 0 734 215 704 696.4 0 704 216 557 696.4 0 557 217 212 696.4 0 212 218 298 696.4 0 298 219 1221 696.4 0 1221 220 768 696.4 0 768 221 904 696.4 0 904 222 357 696.4 0 357 223 178 696.4 0 178 224 70 696.4 0 70 225 96 696.4 0 96 226 89 696.4 0 89 227 110 696.4 0 110 228 96 696.4 0 96 229 156 696.4 0 156 230 78 696.4 0 78 231 87 696.4 0 87 232 66 696.4 0 66 233 104 696.4 0 104 234 89 696.4 0 89 235 85 696.4 0 85 236 184 696.4 0 184 237 129 696.4 0 129 238 238 696.4 0 238 239 1048 696.4 0 1048 240 1127 696.4 0 1127 241 988 696.4 0 988 242 349 696.4 0 349 243 58 696.4 0 58 244 97 696.4 0 97 245 91 696.4 0 91 246 163 696.4 0 163 247 92 696.4 0 92 248 110 696.4 0 110 249 200 696.4 0 200 250 572 696.4 0 572 251 1474 696.4 0 1474 252 1252 696.4 0 1252 253 1277 696.4 0 1277 254 71 696.4 0 71 255 106 696.4 0 106 256 127 696.4 0 127 257 179 696.4 0 179 258 151 696.4 0 151 259 356 696.4 0 356 260 214 696.4 0 214 261 374 696.4 0 374 262 146 696.4 0 146 263 842 696.4 0 842 264 464 696.4 0 464 265 1139 696.4 0 1139 266 529 696.4 0 529 267 341 696.4 0 341 268 351 696.4 0 351 269 217 696.4 0 217 270 319 696.4 0 319 271 155 696.4 0 155 272 159 696.4 0 159 273 65 696.4 0 65 274 98 696.4 0 98 275 193 696.4 0 193 276 132 696.4 0 132 277 138 696.4 0 138 278 145 696.4 0 145 279 101 696.4 0 101 280 93 696.4 0 93 281 163 696.4 0 163 282 192 696.4 0 192 283 345 696.4 0 345 284 243 696.4 0 243 285 291 696.4 0 291 286 299 696.4 0 299 287 680 696.4 0 680 288 5732 696.4 0 5732 289 4319 696.4 0 4319 290 4915 696.4 0 4915 291 464 696.4 0 464 292 167 696.4 0 167 293 317 696.4 0 317 294 432 696.4 0 432 295 296 696.4 0 296 296 165 696.4 0 165 297 282 696.4 0 282 298 244 696.4 0 244 299 380 696.4 0 380 300 389 696.4 0 389 301 299 696.4 0 299 Finished in 19.77 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 474,242 Reads with adapters: 43,951 (9.3%) Reads that were too short: 3,294 (0.7%) Reads written (passing filters): 40,657 (8.6%) Total basepairs processed: 121,482,067 bp Total written (filtered): 9,816,029 bp (8.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 43951 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 12 0.0 3 0 0 9 3 16 246 0.0 3 0 213 23 10 17 30158 0.0 3 28300 1542 260 56 18 97 0.0 3 5 76 10 6 19 52 0.0 3 0 0 28 24 20 147 0.0 3 0 1 0 146 22 1 0.0 3 0 0 0 1 23 2 0.0 3 1 0 0 1 25 1 0.0 3 1 27 2 0.0 3 0 0 1 1 28 5 0.0 3 0 0 0 5 29 8 0.0 3 0 0 0 8 30 1 0.0 3 0 0 0 1 31 3 0.0 3 0 0 0 3 32 4 0.0 3 0 0 0 4 33 3 0.0 3 0 0 0 3 34 10 0.0 3 0 0 1 9 35 12 0.0 3 0 0 0 12 38 2 0.0 3 1 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 9 0.0 3 0 0 1 8 48 2 0.0 3 1 0 0 1 49 3 0.0 3 0 0 0 3 50 6 0.0 3 0 0 0 6 51 14 0.0 3 0 0 0 14 52 5 0.0 3 0 0 0 5 53 4 0.0 3 0 0 1 3 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 1 71 4 0.0 3 0 0 0 4 72 14 0.0 3 0 0 0 14 73 962 0.0 3 0 0 9 953 74 113 0.0 3 0 0 2 111 75 13 0.0 3 0 0 0 13 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 4 0.0 3 0 0 0 4 80 8 0.0 3 0 0 0 8 81 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 0 2 87 65 0.0 3 0 0 0 65 89 10 0.0 3 0 0 0 10 90 14 0.0 3 0 0 0 14 91 4 0.0 3 0 0 0 4 92 26 0.0 3 0 0 0 26 93 9 0.0 3 0 0 2 7 95 2 0.0 3 0 0 1 1 96 3 0.0 3 0 0 0 3 97 16 0.0 3 0 0 0 16 98 4 0.0 3 0 0 0 4 99 4 0.0 3 0 0 0 4 100 10 0.0 3 0 0 0 10 101 9 0.0 3 0 0 0 9 102 83 0.0 3 0 0 0 83 103 23 0.0 3 0 0 0 23 104 9 0.0 3 0 0 0 9 105 1 0.0 3 0 0 0 1 108 4 0.0 3 0 0 0 4 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 4 0.0 3 0 0 0 4 114 2 0.0 3 0 0 0 2 116 5 0.0 3 0 0 0 5 117 15 0.0 3 0 0 0 15 118 3 0.0 3 0 0 0 3 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 1 1 121 8 0.0 3 0 0 0 8 122 2547 0.0 3 0 0 35 2512 123 1372 0.0 3 0 0 11 1361 124 389 0.0 3 0 0 2 387 125 685 0.0 3 0 1 18 666 126 214 0.0 3 0 0 8 206 127 66 0.0 3 0 0 3 63 128 250 0.0 3 0 0 8 242 129 10 0.0 3 0 0 1 9 130 64 0.0 3 0 0 0 64 131 56 0.0 3 0 0 2 54 132 9 0.0 3 0 1 0 8 133 33 0.0 3 0 0 1 32 134 13 0.0 3 0 0 0 13 135 13 0.0 3 0 0 1 12 136 21 0.0 3 0 0 2 19 137 111 0.0 3 0 0 2 109 138 69 0.0 3 0 0 1 68 139 80 0.0 3 0 0 1 79 140 42 0.0 3 0 0 2 40 141 27 0.0 3 0 0 0 27 142 550 0.0 3 0 0 4 546 143 35 0.0 3 0 0 0 35 144 12 0.0 3 0 0 0 12 145 61 0.0 3 0 0 1 60 146 44 0.0 3 0 0 0 44 147 8 0.0 3 0 0 0 8 148 66 0.0 3 0 0 0 66 149 58 0.0 3 0 0 1 57 150 16 0.0 3 0 0 0 16 151 498 0.0 3 0 0 12 486 152 98 0.0 3 0 0 0 98 153 46 0.0 3 0 0 3 43 154 93 0.0 3 0 0 3 90 155 23 0.0 3 0 0 0 23 156 9 0.0 3 0 0 0 9 157 13 0.0 3 0 0 0 13 158 4 0.0 3 0 0 0 4 159 8 0.0 3 0 0 0 8 160 5 0.0 3 0 0 1 4 161 4 0.0 3 0 0 0 4 162 5 0.0 3 0 0 0 5 163 9 0.0 3 0 0 0 9 164 11 0.0 3 0 0 1 10 165 8 0.0 3 0 0 0 8 166 19 0.0 3 0 0 0 19 167 10 0.0 3 0 1 1 8 168 28 0.0 3 0 0 1 27 169 9 0.0 3 0 0 0 9 170 14 0.0 3 0 0 0 14 171 456 0.0 3 0 0 9 447 172 151 0.0 3 0 0 2 149 173 51 0.0 3 0 0 2 49 174 101 0.0 3 0 0 2 99 175 51 0.0 3 0 0 0 51 176 9 0.0 3 0 0 0 9 177 49 0.0 3 0 0 0 49 178 8 0.0 3 0 0 0 8 179 4 0.0 3 0 0 1 3 180 10 0.0 3 0 0 0 10 181 9 0.0 3 0 0 0 9 182 2 0.0 3 0 0 0 2 183 20 0.0 3 0 0 1 19 184 4 0.0 3 0 0 0 4 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 188 7 0.0 3 0 0 1 6 189 5 0.0 3 0 0 0 5 190 5 0.0 3 0 0 0 5 191 7 0.0 3 0 0 0 7 192 3 0.0 3 0 0 0 3 193 21 0.0 3 0 0 1 20 194 9 0.0 3 0 0 1 8 195 9 0.0 3 0 0 0 9 196 4 0.0 3 0 0 0 4 197 11 0.0 3 0 0 0 11 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 14 0.0 3 0 0 0 14 203 5 0.0 3 0 0 0 5 204 8 0.0 3 0 0 0 8 205 13 0.0 3 0 0 1 12 206 8 0.0 3 0 0 0 8 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 211 3 0.0 3 0 0 0 3 212 3 0.0 3 0 0 0 3 213 1 0.0 3 0 0 0 1 214 4 0.0 3 0 0 1 3 215 9 0.0 3 0 0 0 9 216 1 0.0 3 0 0 0 1 217 13 0.0 3 0 0 0 13 220 5 0.0 3 0 0 0 5 221 3 0.0 3 0 0 0 3 222 12 0.0 3 0 0 1 11 223 12 0.0 3 0 0 1 11 224 9 0.0 3 0 0 0 9 225 14 0.0 3 0 0 1 13 226 3 0.0 3 0 0 0 3 227 9 0.0 3 0 0 0 9 228 3 0.0 3 0 0 0 3 229 9 0.0 3 0 0 0 9 230 2 0.0 3 0 0 0 2 231 18 0.0 3 0 0 0 18 232 1 0.0 3 0 0 1 233 10 0.0 3 0 0 0 10 234 770 0.0 3 0 0 6 764 235 310 0.0 3 0 0 4 306 236 141 0.0 3 0 0 5 136 237 231 0.0 3 0 0 9 222 238 344 0.0 3 0 1 10 333 239 84 0.0 3 0 0 1 83 240 71 0.0 3 0 0 5 66 241 19 0.0 3 0 0 1 18 242 33 0.0 3 0 0 2 31 243 85 0.0 3 0 0 5 80 244 72 0.0 3 0 0 5 67 245 18 0.0 3 0 0 1 17 246 9 0.0 3 0 0 0 9 247 7 0.0 3 0 0 0 7 248 8 0.0 3 0 0 1 7 249 4 0.0 3 0 0 0 4 250 18 0.0 3 0 0 0 18 251 12 0.0 3 0 0 1 11 252 44 0.0 3 0 0 3 41 253 10 0.0 3 0 0 0 10 254 16 0.0 3 0 0 0 16 255 2 0.0 3 0 0 0 2 256 3 0.0 3 0 0 0 3 259 3 0.0 3 0 0 0 3 260 2 0.0 3 0 0 0 2 262 8 0.0 3 0 0 1 7 263 77 0.0 3 0 0 0 77 264 35 0.0 3 0 0 1 34 265 17 0.0 3 0 0 0 17 266 26 0.0 3 0 0 2 24 267 25 0.0 3 0 0 0 25 268 10 0.0 3 0 0 2 8 269 3 0.0 3 0 0 0 3 270 3 0.0 3 0 0 0 3 271 6 0.0 3 0 0 0 6 272 30 0.0 3 0 0 1 29 273 18 0.0 3 0 0 0 18 274 4 0.0 3 0 0 0 4 275 1 0.0 3 0 0 0 1 278 3 0.0 3 0 0 0 3 279 6 0.0 3 0 0 0 6 280 2 0.0 3 0 0 0 2 281 16 0.0 3 0 0 1 15 282 4 0.0 3 0 0 0 4 288 1 0.0 3 0 0 0 1 291 3 0.0 3 0 0 0 3 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 40,657 Reads with adapters: 724 (1.8%) Reads written (passing filters): 40,657 (100.0%) Total basepairs processed: 9,816,029 bp Total written (filtered): 9,813,795 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 724 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.0% C: 30.4% G: 11.9% T: 3.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 691 635.3 0 691 4 16 158.8 0 16 5 11 39.7 0 11 6 5 9.9 0 5 12 1 0.0 1 0 1