This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.12 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 411,828 (14.4%) Reads that were too short: 12,368 (0.4%) Reads written (passing filters): 399,460 (14.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 97,795,460 bp (11.4%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 411828 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5659 696.4 0 5659 7 86433 696.4 0 86433 8 1692 696.4 0 1692 9 78029 696.4 0 78029 10 108 696.4 0 108 11 60 696.4 0 60 12 63 696.4 0 63 13 57 696.4 0 57 14 41 696.4 0 41 15 64 696.4 0 64 16 67 696.4 0 67 17 63 696.4 0 63 18 80 696.4 0 80 19 61 696.4 0 61 20 68 696.4 0 68 21 63 696.4 0 63 22 71 696.4 0 71 23 46 696.4 0 46 24 83 696.4 0 83 25 51 696.4 0 51 26 59 696.4 0 59 27 57 696.4 0 57 28 47 696.4 0 47 29 47 696.4 0 47 30 52 696.4 0 52 31 43 696.4 0 43 32 55 696.4 0 55 33 52 696.4 0 52 34 42 696.4 0 42 35 70 696.4 0 70 36 59 696.4 0 59 37 60 696.4 0 60 38 38 696.4 0 38 39 57 696.4 0 57 40 61 696.4 0 61 41 54 696.4 0 54 42 66 696.4 0 66 43 67 696.4 0 67 44 65 696.4 0 65 45 78 696.4 0 78 46 66 696.4 0 66 47 66 696.4 0 66 48 46 696.4 0 46 49 64 696.4 0 64 50 40 696.4 0 40 51 78 696.4 0 78 52 69 696.4 0 69 53 81 696.4 0 81 54 100 696.4 0 100 55 97 696.4 0 97 56 132 696.4 0 132 57 102 696.4 0 102 58 69 696.4 0 69 59 54 696.4 0 54 60 89 696.4 0 89 61 92 696.4 0 92 62 151 696.4 0 151 63 100 696.4 0 100 64 81 696.4 0 81 65 81 696.4 0 81 66 156 696.4 0 156 67 89 696.4 0 89 68 164 696.4 0 164 69 207 696.4 0 207 70 253 696.4 0 253 71 308 696.4 0 308 72 685 696.4 0 685 73 1460 696.4 0 1460 74 439 696.4 0 439 75 491 696.4 0 491 76 1138 696.4 0 1138 77 382 696.4 0 382 78 421 696.4 0 421 79 962 696.4 0 962 80 952 696.4 0 952 81 1436 696.4 0 1436 82 896 696.4 0 896 83 1604 696.4 0 1604 84 1784 696.4 0 1784 85 3765 696.4 0 3765 86 72403 696.4 0 72403 87 52005 696.4 0 52005 88 57613 696.4 0 57613 89 246 696.4 0 246 90 63 696.4 0 63 91 36 696.4 0 36 92 29 696.4 0 29 93 37 696.4 0 37 94 41 696.4 0 41 95 43 696.4 0 43 96 82 696.4 0 82 97 28 696.4 0 28 98 49 696.4 0 49 99 72 696.4 0 72 100 58 696.4 0 58 101 57 696.4 0 57 102 53 696.4 0 53 103 91 696.4 0 91 104 42 696.4 0 42 105 68 696.4 0 68 106 39 696.4 0 39 107 30 696.4 0 30 108 58 696.4 0 58 109 34 696.4 0 34 110 48 696.4 0 48 111 56 696.4 0 56 112 52 696.4 0 52 113 43 696.4 0 43 114 46 696.4 0 46 115 34 696.4 0 34 116 49 696.4 0 49 117 57 696.4 0 57 118 49 696.4 0 49 119 52 696.4 0 52 120 62 696.4 0 62 121 61 696.4 0 61 122 61 696.4 0 61 123 232 696.4 0 232 124 375 696.4 0 375 125 300 696.4 0 300 126 425 696.4 0 425 127 150 696.4 0 150 128 61 696.4 0 61 129 172 696.4 0 172 130 94 696.4 0 94 131 109 696.4 0 109 132 58 696.4 0 58 133 75 696.4 0 75 134 108 696.4 0 108 135 76 696.4 0 76 136 101 696.4 0 101 137 105 696.4 0 105 138 90 696.4 0 90 139 128 696.4 0 128 140 86 696.4 0 86 141 184 696.4 0 184 142 117 696.4 0 117 143 208 696.4 0 208 144 3222 696.4 0 3222 145 2648 696.4 0 2648 146 2666 696.4 0 2666 147 88 696.4 0 88 148 62 696.4 0 62 149 85 696.4 0 85 150 63 696.4 0 63 151 83 696.4 0 83 152 42 696.4 0 42 153 55 696.4 0 55 154 60 696.4 0 60 155 64 696.4 0 64 156 66 696.4 0 66 157 80 696.4 0 80 158 67 696.4 0 67 159 63 696.4 0 63 160 63 696.4 0 63 161 80 696.4 0 80 162 54 696.4 0 54 163 84 696.4 0 84 164 79 696.4 0 79 165 117 696.4 0 117 166 1156 696.4 0 1156 167 796 696.4 0 796 168 992 696.4 0 992 169 63 696.4 0 63 170 60 696.4 0 60 171 67 696.4 0 67 172 108 696.4 0 108 173 62 696.4 0 62 174 61 696.4 0 61 175 81 696.4 0 81 176 77 696.4 0 77 177 64 696.4 0 64 178 78 696.4 0 78 179 70 696.4 0 70 180 96 696.4 0 96 181 83 696.4 0 83 182 94 696.4 0 94 183 107 696.4 0 107 184 136 696.4 0 136 185 2716 696.4 0 2716 186 655 696.4 0 655 187 447 696.4 0 447 188 78 696.4 0 78 189 101 696.4 0 101 190 52 696.4 0 52 191 53 696.4 0 53 192 65 696.4 0 65 193 102 696.4 0 102 194 71 696.4 0 71 195 77 696.4 0 77 196 79 696.4 0 79 197 64 696.4 0 64 198 66 696.4 0 66 199 82 696.4 0 82 200 87 696.4 0 87 201 84 696.4 0 84 202 79 696.4 0 79 203 114 696.4 0 114 204 134 696.4 0 134 205 159 696.4 0 159 206 2247 696.4 0 2247 207 1253 696.4 0 1253 208 1654 696.4 0 1654 209 71 696.4 0 71 210 53 696.4 0 53 211 404 696.4 0 404 212 288 696.4 0 288 213 293 696.4 0 293 214 73 696.4 0 73 215 41 696.4 0 41 216 54 696.4 0 54 217 57 696.4 0 57 218 41 696.4 0 41 219 39 696.4 0 39 220 52 696.4 0 52 221 63 696.4 0 63 222 73 696.4 0 73 223 49 696.4 0 49 224 49 696.4 0 49 225 66 696.4 0 66 226 82 696.4 0 82 227 76 696.4 0 76 228 128 696.4 0 128 229 59 696.4 0 59 230 42 696.4 0 42 231 51 696.4 0 51 232 57 696.4 0 57 233 35 696.4 0 35 234 44 696.4 0 44 235 40 696.4 0 40 236 35 696.4 0 35 237 49 696.4 0 49 238 58 696.4 0 58 239 44 696.4 0 44 240 62 696.4 0 62 241 64 696.4 0 64 242 60 696.4 0 60 243 74 696.4 0 74 244 37 696.4 0 37 245 71 696.4 0 71 246 68 696.4 0 68 247 52 696.4 0 52 248 66 696.4 0 66 249 52 696.4 0 52 250 35 696.4 0 35 251 42 696.4 0 42 252 30 696.4 0 30 253 40 696.4 0 40 254 52 696.4 0 52 255 72 696.4 0 72 256 58 696.4 0 58 257 73 696.4 0 73 258 43 696.4 0 43 259 149 696.4 0 149 260 104 696.4 0 104 261 231 696.4 0 231 262 104 696.4 0 104 263 46 696.4 0 46 264 54 696.4 0 54 265 40 696.4 0 40 266 57 696.4 0 57 267 96 696.4 0 96 268 107 696.4 0 107 269 86 696.4 0 86 270 57 696.4 0 57 271 54 696.4 0 54 272 57 696.4 0 57 273 49 696.4 0 49 274 51 696.4 0 51 275 116 696.4 0 116 276 66 696.4 0 66 277 62 696.4 0 62 278 36 696.4 0 36 279 79 696.4 0 79 280 68 696.4 0 68 281 87 696.4 0 87 282 104 696.4 0 104 283 56 696.4 0 56 284 47 696.4 0 47 285 42 696.4 0 42 286 36 696.4 0 36 287 56 696.4 0 56 288 53 696.4 0 53 289 56 696.4 0 56 290 36 696.4 0 36 291 41 696.4 0 41 292 42 696.4 0 42 293 56 696.4 0 56 294 92 696.4 0 92 295 101 696.4 0 101 296 91 696.4 0 91 297 83 696.4 0 83 298 47 696.4 0 47 299 46 696.4 0 46 300 62 696.4 0 62 301 108 696.4 0 108 Finished in 17.90 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 399,460 Reads with adapters: 89,401 (22.4%) Reads that were too short: 86 (0.0%) Reads written (passing filters): 89,315 (22.4%) Total basepairs processed: 97,795,460 bp Total written (filtered): 24,432,325 bp (25.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 89401 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 39 0.0 3 0 0 38 1 19 1700 0.0 3 0 1668 25 4 3 20 85204 0.0 4 83676 1489 35 3 1 21 161 0.0 4 30 128 3 22 1802 0.0 4 1754 37 8 0 3 23 40 0.0 4 3 3 0 30 4 25 2 0.0 4 0 0 0 2 27 1 0.0 4 0 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 31 1 0.0 4 1 36 1 0.0 4 0 1 42 1 0.0 4 1 48 10 0.0 4 0 0 0 0 10 51 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 74 15 0.0 4 0 0 0 0 15 86 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 104 1 0.0 4 1 106 2 0.0 4 2 107 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 298 0.0 4 0 0 0 0 298 128 3 0.0 4 0 0 0 0 3 129 24 0.0 4 0 0 0 0 24 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 36 0.0 4 0 0 0 0 36 150 3 0.0 4 0 0 0 0 3 151 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 154 2 0.0 4 0 0 0 0 2 155 3 0.0 4 0 0 0 1 2 156 2 0.0 4 0 0 0 0 2 179 1 0.0 4 0 0 0 0 1 181 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 1 198 1 0.0 4 1 200 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 242 1 0.0 4 1 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.07 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 89,315 Reads with adapters: 1,956 (2.2%) Reads written (passing filters): 89,315 (100.0%) Total basepairs processed: 24,432,325 bp Total written (filtered): 24,405,006 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1956 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.0% C: 5.3% G: 85.6% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 126 1395.5 0 126 4 11 348.9 0 11 5 5 87.2 0 5 8 1 1.4 0 1 9 182 0.3 0 6 176 10 116 0.1 1 3 113 11 1422 0.0 1 22 1400 12 44 0.0 1 3 41 13 4 0.0 1 0 4 14 1 0.0 1 0 1 15 4 0.0 1 0 4 20 3 0.0 1 2 1 110 1 0.0 1 0 1 145 1 0.0 1 1 152 6 0.0 1 0 6 191 1 0.0 1 0 1 210 20 0.0 1 5 15 269 8 0.0 1 8 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.