This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.43 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 140,030 (4.9%) Reads that were too short: 36,135 (1.3%) Reads written (passing filters): 103,895 (3.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,857,620 bp (3.0%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 140030 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 19377 696.4 0 19377 7 11316 696.4 0 11316 8 15793 696.4 0 15793 9 14010 696.4 0 14010 10 118 696.4 0 118 11 120 696.4 0 120 12 72 696.4 0 72 13 80 696.4 0 80 14 74 696.4 0 74 15 107 696.4 0 107 16 85 696.4 0 85 17 73 696.4 0 73 18 81 696.4 0 81 19 89 696.4 0 89 20 75 696.4 0 75 21 73 696.4 0 73 22 72 696.4 0 72 23 88 696.4 0 88 24 97 696.4 0 97 25 73 696.4 0 73 26 85 696.4 0 85 27 139 696.4 0 139 28 120 696.4 0 120 29 66 696.4 0 66 30 90 696.4 0 90 31 81 696.4 0 81 32 109 696.4 0 109 33 113 696.4 0 113 34 80 696.4 0 80 35 132 696.4 0 132 36 150 696.4 0 150 37 1090 696.4 0 1090 38 1471 696.4 0 1471 39 1694 696.4 0 1694 40 290 696.4 0 290 41 95 696.4 0 95 42 75 696.4 0 75 43 110 696.4 0 110 44 113 696.4 0 113 45 92 696.4 0 92 46 82 696.4 0 82 47 99 696.4 0 99 48 98 696.4 0 98 49 94 696.4 0 94 50 82 696.4 0 82 51 96 696.4 0 96 52 69 696.4 0 69 53 110 696.4 0 110 54 92 696.4 0 92 55 86 696.4 0 86 56 85 696.4 0 85 57 95 696.4 0 95 58 87 696.4 0 87 59 92 696.4 0 92 60 69 696.4 0 69 61 69 696.4 0 69 62 92 696.4 0 92 63 65 696.4 0 65 64 62 696.4 0 62 65 77 696.4 0 77 66 86 696.4 0 86 67 89 696.4 0 89 68 87 696.4 0 87 69 98 696.4 0 98 70 116 696.4 0 116 71 73 696.4 0 73 72 96 696.4 0 96 73 86 696.4 0 86 74 104 696.4 0 104 75 281 696.4 0 281 76 200 696.4 0 200 77 100 696.4 0 100 78 133 696.4 0 133 79 136 696.4 0 136 80 129 696.4 0 129 81 117 696.4 0 117 82 104 696.4 0 104 83 92 696.4 0 92 84 87 696.4 0 87 85 65 696.4 0 65 86 86 696.4 0 86 87 69 696.4 0 69 88 95 696.4 0 95 89 99 696.4 0 99 90 185 696.4 0 185 91 255 696.4 0 255 92 562 696.4 0 562 93 501 696.4 0 501 94 325 696.4 0 325 95 205 696.4 0 205 96 86 696.4 0 86 97 104 696.4 0 104 98 87 696.4 0 87 99 65 696.4 0 65 100 77 696.4 0 77 101 85 696.4 0 85 102 74 696.4 0 74 103 95 696.4 0 95 104 90 696.4 0 90 105 79 696.4 0 79 106 85 696.4 0 85 107 93 696.4 0 93 108 91 696.4 0 91 109 111 696.4 0 111 110 117 696.4 0 117 111 81 696.4 0 81 112 242 696.4 0 242 113 206 696.4 0 206 114 246 696.4 0 246 115 173 696.4 0 173 116 112 696.4 0 112 117 114 696.4 0 114 118 117 696.4 0 117 119 90 696.4 0 90 120 103 696.4 0 103 121 84 696.4 0 84 122 122 696.4 0 122 123 105 696.4 0 105 124 100 696.4 0 100 125 112 696.4 0 112 126 103 696.4 0 103 127 131 696.4 0 131 128 170 696.4 0 170 129 336 696.4 0 336 130 484 696.4 0 484 131 289 696.4 0 289 132 176 696.4 0 176 133 166 696.4 0 166 134 145 696.4 0 145 135 161 696.4 0 161 136 213 696.4 0 213 137 217 696.4 0 217 138 175 696.4 0 175 139 224 696.4 0 224 140 296 696.4 0 296 141 451 696.4 0 451 142 4377 696.4 0 4377 143 6369 696.4 0 6369 144 6229 696.4 0 6229 145 108 696.4 0 108 146 64 696.4 0 64 147 91 696.4 0 91 148 116 696.4 0 116 149 241 696.4 0 241 150 224 696.4 0 224 151 231 696.4 0 231 152 68 696.4 0 68 153 85 696.4 0 85 154 76 696.4 0 76 155 87 696.4 0 87 156 94 696.4 0 94 157 108 696.4 0 108 158 81 696.4 0 81 159 82 696.4 0 82 160 78 696.4 0 78 161 58 696.4 0 58 162 83 696.4 0 83 163 78 696.4 0 78 164 89 696.4 0 89 165 90 696.4 0 90 166 84 696.4 0 84 167 78 696.4 0 78 168 76 696.4 0 76 169 105 696.4 0 105 170 107 696.4 0 107 171 92 696.4 0 92 172 72 696.4 0 72 173 103 696.4 0 103 174 83 696.4 0 83 175 88 696.4 0 88 176 82 696.4 0 82 177 114 696.4 0 114 178 137 696.4 0 137 179 140 696.4 0 140 180 82 696.4 0 82 181 52 696.4 0 52 182 215 696.4 0 215 183 75 696.4 0 75 184 73 696.4 0 73 185 69 696.4 0 69 186 72 696.4 0 72 187 63 696.4 0 63 188 81 696.4 0 81 189 85 696.4 0 85 190 96 696.4 0 96 191 74 696.4 0 74 192 78 696.4 0 78 193 61 696.4 0 61 194 70 696.4 0 70 195 64 696.4 0 64 196 67 696.4 0 67 197 85 696.4 0 85 198 92 696.4 0 92 199 71 696.4 0 71 200 85 696.4 0 85 201 104 696.4 0 104 202 104 696.4 0 104 203 125 696.4 0 125 204 121 696.4 0 121 205 101 696.4 0 101 206 72 696.4 0 72 207 89 696.4 0 89 208 126 696.4 0 126 209 506 696.4 0 506 210 261 696.4 0 261 211 361 696.4 0 361 212 199 696.4 0 199 213 256 696.4 0 256 214 1649 696.4 0 1649 215 1069 696.4 0 1069 216 2663 696.4 0 2663 217 336 696.4 0 336 218 255 696.4 0 255 219 82 696.4 0 82 220 92 696.4 0 92 221 142 696.4 0 142 222 115 696.4 0 115 223 135 696.4 0 135 224 150 696.4 0 150 225 91 696.4 0 91 226 154 696.4 0 154 227 192 696.4 0 192 228 241 696.4 0 241 229 696 696.4 0 696 230 453 696.4 0 453 231 665 696.4 0 665 232 323 696.4 0 323 233 470 696.4 0 470 234 4311 696.4 0 4311 235 4547 696.4 0 4547 236 5261 696.4 0 5261 237 1327 696.4 0 1327 238 79 696.4 0 79 239 68 696.4 0 68 240 70 696.4 0 70 241 81 696.4 0 81 242 69 696.4 0 69 243 86 696.4 0 86 244 83 696.4 0 83 245 66 696.4 0 66 246 67 696.4 0 67 247 78 696.4 0 78 248 87 696.4 0 87 249 71 696.4 0 71 250 73 696.4 0 73 251 73 696.4 0 73 252 88 696.4 0 88 253 238 696.4 0 238 254 291 696.4 0 291 255 305 696.4 0 305 256 244 696.4 0 244 257 81 696.4 0 81 258 78 696.4 0 78 259 81 696.4 0 81 260 101 696.4 0 101 261 107 696.4 0 107 262 362 696.4 0 362 263 270 696.4 0 270 264 235 696.4 0 235 265 103 696.4 0 103 266 55 696.4 0 55 267 66 696.4 0 66 268 47 696.4 0 47 269 66 696.4 0 66 270 112 696.4 0 112 271 88 696.4 0 88 272 119 696.4 0 119 273 70 696.4 0 70 274 58 696.4 0 58 275 85 696.4 0 85 276 118 696.4 0 118 277 144 696.4 0 144 278 155 696.4 0 155 279 91 696.4 0 91 280 63 696.4 0 63 281 78 696.4 0 78 282 100 696.4 0 100 283 75 696.4 0 75 284 151 696.4 0 151 285 111 696.4 0 111 286 74 696.4 0 74 287 51 696.4 0 51 288 49 696.4 0 49 289 58 696.4 0 58 290 43 696.4 0 43 291 93 696.4 0 93 292 84 696.4 0 84 293 114 696.4 0 114 294 65 696.4 0 65 295 202 696.4 0 202 296 429 696.4 0 429 297 693 696.4 0 693 298 435 696.4 0 435 299 342 696.4 0 342 300 52 696.4 0 52 301 124 696.4 0 124 Finished in 4.64 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 103,895 Reads with adapters: 33,641 (32.4%) Reads that were too short: 110 (0.1%) Reads written (passing filters): 33,531 (32.3%) Total basepairs processed: 25,857,620 bp Total written (filtered): 9,251,709 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 33641 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 6 0.0 3 0 0 3 3 16 148 0.0 3 0 113 22 13 17 15784 0.0 3 14516 1041 158 69 18 17143 0.0 3 16001 935 154 53 19 107 0.0 3 21 31 31 24 20 94 0.0 3 2 1 22 69 21 112 0.0 3 1 0 0 111 22 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 1 42 13 0.0 3 0 0 0 13 44 1 0.0 3 0 0 0 1 45 9 0.0 3 0 0 0 9 47 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 27 0.0 3 0 0 1 26 51 2 0.0 3 0 0 0 2 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 4 0.0 3 0 0 0 4 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 4 0.0 3 0 0 0 4 99 1 0.0 3 0 0 0 1 100 5 0.0 3 0 0 0 5 101 5 0.0 3 0 0 1 4 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 110 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 7 0.0 3 0 0 0 7 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 3 0.0 3 0 0 0 3 153 2 0.0 3 0 0 0 2 154 10 0.0 3 0 0 0 10 155 9 0.0 3 0 0 0 9 156 1 0.0 3 0 0 0 1 157 6 0.0 3 0 0 0 6 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 195 4 0.0 3 0 0 0 4 196 1 0.0 3 0 0 1 201 1 0.0 3 0 0 0 1 205 6 0.0 3 0 0 0 6 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 214 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 11 0.0 3 0 0 0 11 218 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 0 1 256 3 0.0 3 0 0 0 3 261 1 0.0 3 0 0 0 1 262 6 0.0 3 0 0 0 6 269 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.18 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 33,531 Reads with adapters: 268 (0.8%) Reads written (passing filters): 33,531 (100.0%) Total basepairs processed: 9,251,709 bp Total written (filtered): 9,250,869 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 268 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.5% C: 32.8% G: 31.0% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 247 523.9 0 247 4 14 131.0 0 14 5 5 32.7 0 5 6 1 8.2 0 1 12 1 0.0 1 0 1