This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCTTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.69 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 118,403 (4.2%) Reads that were too short: 19,496 (0.7%) Reads written (passing filters): 98,907 (3.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,901,433 bp (2.9%) === Adapter 1 === Sequence: TCTTGG; Type: regular 5'; Length: 6; Trimmed: 118403 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 18134 696.4 0 18134 7 12918 696.4 0 12918 8 15692 696.4 0 15692 9 15497 696.4 0 15497 10 74 696.4 0 74 11 82 696.4 0 82 12 44 696.4 0 44 13 54 696.4 0 54 14 53 696.4 0 53 15 46 696.4 0 46 16 54 696.4 0 54 17 73 696.4 0 73 18 55 696.4 0 55 19 74 696.4 0 74 20 58 696.4 0 58 21 68 696.4 0 68 22 46 696.4 0 46 23 59 696.4 0 59 24 83 696.4 0 83 25 52 696.4 0 52 26 83 696.4 0 83 27 68 696.4 0 68 28 79 696.4 0 79 29 60 696.4 0 60 30 69 696.4 0 69 31 68 696.4 0 68 32 64 696.4 0 64 33 80 696.4 0 80 34 84 696.4 0 84 35 90 696.4 0 90 36 80 696.4 0 80 37 723 696.4 0 723 38 994 696.4 0 994 39 1011 696.4 0 1011 40 66 696.4 0 66 41 72 696.4 0 72 42 75 696.4 0 75 43 69 696.4 0 69 44 80 696.4 0 80 45 75 696.4 0 75 46 73 696.4 0 73 47 69 696.4 0 69 48 69 696.4 0 69 49 70 696.4 0 70 50 59 696.4 0 59 51 64 696.4 0 64 52 58 696.4 0 58 53 72 696.4 0 72 54 67 696.4 0 67 55 52 696.4 0 52 56 63 696.4 0 63 57 67 696.4 0 67 58 77 696.4 0 77 59 55 696.4 0 55 60 53 696.4 0 53 61 61 696.4 0 61 62 67 696.4 0 67 63 70 696.4 0 70 64 61 696.4 0 61 65 75 696.4 0 75 66 53 696.4 0 53 67 57 696.4 0 57 68 63 696.4 0 63 69 70 696.4 0 70 70 72 696.4 0 72 71 72 696.4 0 72 72 64 696.4 0 64 73 58 696.4 0 58 74 78 696.4 0 78 75 248 696.4 0 248 76 177 696.4 0 177 77 59 696.4 0 59 78 91 696.4 0 91 79 56 696.4 0 56 80 83 696.4 0 83 81 90 696.4 0 90 82 54 696.4 0 54 83 69 696.4 0 69 84 43 696.4 0 43 85 49 696.4 0 49 86 48 696.4 0 48 87 52 696.4 0 52 88 61 696.4 0 61 89 48 696.4 0 48 90 117 696.4 0 117 91 152 696.4 0 152 92 273 696.4 0 273 93 337 696.4 0 337 94 152 696.4 0 152 95 145 696.4 0 145 96 65 696.4 0 65 97 67 696.4 0 67 98 53 696.4 0 53 99 65 696.4 0 65 100 65 696.4 0 65 101 53 696.4 0 53 102 58 696.4 0 58 103 75 696.4 0 75 104 82 696.4 0 82 105 52 696.4 0 52 106 70 696.4 0 70 107 74 696.4 0 74 108 65 696.4 0 65 109 65 696.4 0 65 110 81 696.4 0 81 111 59 696.4 0 59 112 186 696.4 0 186 113 187 696.4 0 187 114 130 696.4 0 130 115 113 696.4 0 113 116 112 696.4 0 112 117 89 696.4 0 89 118 94 696.4 0 94 119 53 696.4 0 53 120 57 696.4 0 57 121 65 696.4 0 65 122 79 696.4 0 79 123 76 696.4 0 76 124 55 696.4 0 55 125 68 696.4 0 68 126 95 696.4 0 95 127 125 696.4 0 125 128 133 696.4 0 133 129 263 696.4 0 263 130 497 696.4 0 497 131 247 696.4 0 247 132 193 696.4 0 193 133 116 696.4 0 116 134 107 696.4 0 107 135 143 696.4 0 143 136 185 696.4 0 185 137 174 696.4 0 174 138 142 696.4 0 142 139 189 696.4 0 189 140 217 696.4 0 217 141 352 696.4 0 352 142 4602 696.4 0 4602 143 6698 696.4 0 6698 144 6801 696.4 0 6801 145 91 696.4 0 91 146 53 696.4 0 53 147 61 696.4 0 61 148 57 696.4 0 57 149 134 696.4 0 134 150 145 696.4 0 145 151 154 696.4 0 154 152 63 696.4 0 63 153 69 696.4 0 69 154 48 696.4 0 48 155 61 696.4 0 61 156 58 696.4 0 58 157 59 696.4 0 59 158 60 696.4 0 60 159 51 696.4 0 51 160 40 696.4 0 40 161 49 696.4 0 49 162 53 696.4 0 53 163 72 696.4 0 72 164 66 696.4 0 66 165 52 696.4 0 52 166 62 696.4 0 62 167 40 696.4 0 40 168 56 696.4 0 56 169 72 696.4 0 72 170 78 696.4 0 78 171 72 696.4 0 72 172 56 696.4 0 56 173 71 696.4 0 71 174 53 696.4 0 53 175 59 696.4 0 59 176 66 696.4 0 66 177 61 696.4 0 61 178 94 696.4 0 94 179 93 696.4 0 93 180 67 696.4 0 67 181 43 696.4 0 43 182 220 696.4 0 220 183 55 696.4 0 55 184 51 696.4 0 51 185 51 696.4 0 51 186 44 696.4 0 44 187 46 696.4 0 46 188 41 696.4 0 41 189 51 696.4 0 51 190 50 696.4 0 50 191 52 696.4 0 52 192 56 696.4 0 56 193 50 696.4 0 50 194 56 696.4 0 56 195 50 696.4 0 50 196 47 696.4 0 47 197 45 696.4 0 45 198 55 696.4 0 55 199 35 696.4 0 35 200 43 696.4 0 43 201 79 696.4 0 79 202 76 696.4 0 76 203 72 696.4 0 72 204 58 696.4 0 58 205 76 696.4 0 76 206 46 696.4 0 46 207 57 696.4 0 57 208 77 696.4 0 77 209 214 696.4 0 214 210 121 696.4 0 121 211 166 696.4 0 166 212 90 696.4 0 90 213 117 696.4 0 117 214 594 696.4 0 594 215 463 696.4 0 463 216 946 696.4 0 946 217 174 696.4 0 174 218 109 696.4 0 109 219 42 696.4 0 42 220 73 696.4 0 73 221 84 696.4 0 84 222 71 696.4 0 71 223 71 696.4 0 71 224 74 696.4 0 74 225 74 696.4 0 74 226 97 696.4 0 97 227 118 696.4 0 118 228 150 696.4 0 150 229 461 696.4 0 461 230 259 696.4 0 259 231 406 696.4 0 406 232 151 696.4 0 151 233 201 696.4 0 201 234 1886 696.4 0 1886 235 2515 696.4 0 2515 236 2674 696.4 0 2674 237 952 696.4 0 952 238 67 696.4 0 67 239 49 696.4 0 49 240 49 696.4 0 49 241 65 696.4 0 65 242 39 696.4 0 39 243 54 696.4 0 54 244 58 696.4 0 58 245 55 696.4 0 55 246 36 696.4 0 36 247 55 696.4 0 55 248 41 696.4 0 41 249 51 696.4 0 51 250 50 696.4 0 50 251 52 696.4 0 52 252 37 696.4 0 37 253 89 696.4 0 89 254 115 696.4 0 115 255 127 696.4 0 127 256 101 696.4 0 101 257 27 696.4 0 27 258 55 696.4 0 55 259 45 696.4 0 45 260 57 696.4 0 57 261 82 696.4 0 82 262 503 696.4 0 503 263 265 696.4 0 265 264 245 696.4 0 245 265 71 696.4 0 71 266 44 696.4 0 44 267 48 696.4 0 48 268 66 696.4 0 66 269 45 696.4 0 45 270 81 696.4 0 81 271 62 696.4 0 62 272 81 696.4 0 81 273 40 696.4 0 40 274 43 696.4 0 43 275 46 696.4 0 46 276 79 696.4 0 79 277 122 696.4 0 122 278 134 696.4 0 134 279 93 696.4 0 93 280 57 696.4 0 57 281 57 696.4 0 57 282 47 696.4 0 47 283 57 696.4 0 57 284 63 696.4 0 63 285 74 696.4 0 74 286 55 696.4 0 55 287 47 696.4 0 47 288 47 696.4 0 47 289 31 696.4 0 31 290 44 696.4 0 44 291 84 696.4 0 84 292 58 696.4 0 58 293 87 696.4 0 87 294 59 696.4 0 59 295 137 696.4 0 137 296 294 696.4 0 294 297 396 696.4 0 396 298 222 696.4 0 222 299 116 696.4 0 116 300 32 696.4 0 32 301 93 696.4 0 93 Finished in 4.96 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 98,907 Reads with adapters: 30,475 (30.8%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 30,443 (30.8%) Total basepairs processed: 24,901,433 bp Total written (filtered): 8,263,211 bp (33.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30475 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 1 2 18 7 0.0 3 0 0 6 1 19 129 0.0 3 0 125 4 20 16237 0.0 4 15758 458 18 2 1 21 12704 0.0 4 12425 262 16 0 1 22 64 0.0 4 19 39 6 23 985 0.0 4 957 12 3 12 1 24 14 0.0 4 1 2 0 8 3 26 1 0.0 4 0 0 0 1 27 1 0.0 4 1 42 41 0.0 4 0 0 0 0 41 67 1 0.0 4 0 0 0 0 1 97 43 0.0 4 0 0 0 0 43 105 1 0.0 4 0 0 1 126 2 0.0 4 0 0 0 1 1 127 107 0.0 4 0 0 0 0 107 128 102 0.0 4 0 0 0 0 102 130 1 0.0 4 0 0 0 0 1 154 2 0.0 4 0 0 0 0 2 181 1 0.0 4 1 188 20 0.0 4 0 0 0 0 20 198 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 261 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.09 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 30,443 Reads with adapters: 3,482 (11.4%) Reads written (passing filters): 30,443 (100.0%) Total basepairs processed: 8,263,211 bp Total written (filtered): 8,227,050 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3482 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.5% C: 2.8% G: 93.8% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 475.7 0 16 4 4 118.9 0 4 5 1 29.7 0 1 9 1285 0.1 0 50 1235 10 1348 0.0 1 59 1289 11 812 0.0 1 11 801 12 5 0.0 1 0 5 13 1 0.0 1 0 1 22 1 0.0 1 1 70 1 0.0 1 0 1 150 1 0.0 1 0 1 223 1 0.0 1 1 224 1 0.0 1 0 1 270 3 0.0 1 3 271 1 0.0 1 1 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.