This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.33 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 199,585 (7.0%) Reads that were too short: 12,732 (0.4%) Reads written (passing filters): 186,853 (6.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 52,986,631 bp (6.2%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 199585 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4225 696.4 0 4225 7 91474 696.4 0 91474 8 74525 696.4 0 74525 9 114 696.4 0 114 10 24 696.4 0 24 11 26 696.4 0 26 12 35 696.4 0 35 13 34 696.4 0 34 14 25 696.4 0 25 15 15 696.4 0 15 16 29 696.4 0 29 17 27 696.4 0 27 18 47 696.4 0 47 19 28 696.4 0 28 20 71 696.4 0 71 21 49 696.4 0 49 22 40 696.4 0 40 23 23 696.4 0 23 24 27 696.4 0 27 25 35 696.4 0 35 26 30 696.4 0 30 27 84 696.4 0 84 28 32 696.4 0 32 29 200 696.4 0 200 30 56 696.4 0 56 31 158 696.4 0 158 32 29 696.4 0 29 33 37 696.4 0 37 34 26 696.4 0 26 35 27 696.4 0 27 36 18 696.4 0 18 37 32 696.4 0 32 38 20 696.4 0 20 39 30 696.4 0 30 40 46 696.4 0 46 41 40 696.4 0 40 42 25 696.4 0 25 43 30 696.4 0 30 44 30 696.4 0 30 45 38 696.4 0 38 46 39 696.4 0 39 47 44 696.4 0 44 48 41 696.4 0 41 49 24 696.4 0 24 50 25 696.4 0 25 51 18 696.4 0 18 52 48 696.4 0 48 53 34 696.4 0 34 54 117 696.4 0 117 55 426 696.4 0 426 56 105 696.4 0 105 57 22 696.4 0 22 58 37 696.4 0 37 59 35 696.4 0 35 60 33 696.4 0 33 61 188 696.4 0 188 62 175 696.4 0 175 63 247 696.4 0 247 64 37 696.4 0 37 65 53 696.4 0 53 66 31 696.4 0 31 67 103 696.4 0 103 68 87 696.4 0 87 69 109 696.4 0 109 70 62 696.4 0 62 71 198 696.4 0 198 72 73 696.4 0 73 73 63 696.4 0 63 74 61 696.4 0 61 75 37 696.4 0 37 76 26 696.4 0 26 77 47 696.4 0 47 78 48 696.4 0 48 79 73 696.4 0 73 80 67 696.4 0 67 81 61 696.4 0 61 82 61 696.4 0 61 83 129 696.4 0 129 84 99 696.4 0 99 85 64 696.4 0 64 86 89 696.4 0 89 87 116 696.4 0 116 88 58 696.4 0 58 89 51 696.4 0 51 90 45 696.4 0 45 91 39 696.4 0 39 92 56 696.4 0 56 93 29 696.4 0 29 94 36 696.4 0 36 95 50 696.4 0 50 96 55 696.4 0 55 97 48 696.4 0 48 98 60 696.4 0 60 99 31 696.4 0 31 100 30 696.4 0 30 101 17 696.4 0 17 102 23 696.4 0 23 103 28 696.4 0 28 104 22 696.4 0 22 105 27 696.4 0 27 106 23 696.4 0 23 107 32 696.4 0 32 108 38 696.4 0 38 109 27 696.4 0 27 110 29 696.4 0 29 111 34 696.4 0 34 112 56 696.4 0 56 113 50 696.4 0 50 114 54 696.4 0 54 115 63 696.4 0 63 116 33 696.4 0 33 117 41 696.4 0 41 118 32 696.4 0 32 119 55 696.4 0 55 120 56 696.4 0 56 121 38 696.4 0 38 122 63 696.4 0 63 123 145 696.4 0 145 124 122 696.4 0 122 125 190 696.4 0 190 126 431 696.4 0 431 127 340 696.4 0 340 128 948 696.4 0 948 129 657 696.4 0 657 130 587 696.4 0 587 131 221 696.4 0 221 132 97 696.4 0 97 133 58 696.4 0 58 134 149 696.4 0 149 135 102 696.4 0 102 136 119 696.4 0 119 137 29 696.4 0 29 138 68 696.4 0 68 139 56 696.4 0 56 140 383 696.4 0 383 141 42 696.4 0 42 142 37 696.4 0 37 143 100 696.4 0 100 144 52 696.4 0 52 145 89 696.4 0 89 146 75 696.4 0 75 147 60 696.4 0 60 148 76 696.4 0 76 149 62 696.4 0 62 150 63 696.4 0 63 151 52 696.4 0 52 152 39 696.4 0 39 153 43 696.4 0 43 154 39 696.4 0 39 155 52 696.4 0 52 156 31 696.4 0 31 157 35 696.4 0 35 158 32 696.4 0 32 159 59 696.4 0 59 160 43 696.4 0 43 161 46 696.4 0 46 162 55 696.4 0 55 163 40 696.4 0 40 164 49 696.4 0 49 165 76 696.4 0 76 166 404 696.4 0 404 167 232 696.4 0 232 168 213 696.4 0 213 169 58 696.4 0 58 170 39 696.4 0 39 171 50 696.4 0 50 172 43 696.4 0 43 173 51 696.4 0 51 174 65 696.4 0 65 175 75 696.4 0 75 176 71 696.4 0 71 177 52 696.4 0 52 178 35 696.4 0 35 179 31 696.4 0 31 180 50 696.4 0 50 181 28 696.4 0 28 182 62 696.4 0 62 183 64 696.4 0 64 184 71 696.4 0 71 185 68 696.4 0 68 186 68 696.4 0 68 187 62 696.4 0 62 188 50 696.4 0 50 189 54 696.4 0 54 190 63 696.4 0 63 191 61 696.4 0 61 192 84 696.4 0 84 193 68 696.4 0 68 194 83 696.4 0 83 195 89 696.4 0 89 196 209 696.4 0 209 197 489 696.4 0 489 198 398 696.4 0 398 199 399 696.4 0 399 200 70 696.4 0 70 201 53 696.4 0 53 202 66 696.4 0 66 203 57 696.4 0 57 204 38 696.4 0 38 205 61 696.4 0 61 206 63 696.4 0 63 207 72 696.4 0 72 208 80 696.4 0 80 209 74 696.4 0 74 210 87 696.4 0 87 211 131 696.4 0 131 212 120 696.4 0 120 213 122 696.4 0 122 214 70 696.4 0 70 215 85 696.4 0 85 216 57 696.4 0 57 217 116 696.4 0 116 218 145 696.4 0 145 219 172 696.4 0 172 220 370 696.4 0 370 221 183 696.4 0 183 222 201 696.4 0 201 223 79 696.4 0 79 224 62 696.4 0 62 225 61 696.4 0 61 226 63 696.4 0 63 227 64 696.4 0 64 228 64 696.4 0 64 229 62 696.4 0 62 230 41 696.4 0 41 231 85 696.4 0 85 232 65 696.4 0 65 233 97 696.4 0 97 234 71 696.4 0 71 235 53 696.4 0 53 236 81 696.4 0 81 237 72 696.4 0 72 238 126 696.4 0 126 239 127 696.4 0 127 240 168 696.4 0 168 241 161 696.4 0 161 242 175 696.4 0 175 243 63 696.4 0 63 244 97 696.4 0 97 245 114 696.4 0 114 246 138 696.4 0 138 247 139 696.4 0 139 248 150 696.4 0 150 249 170 696.4 0 170 250 103 696.4 0 103 251 115 696.4 0 115 252 69 696.4 0 69 253 76 696.4 0 76 254 225 696.4 0 225 255 179 696.4 0 179 256 136 696.4 0 136 257 96 696.4 0 96 258 68 696.4 0 68 259 71 696.4 0 71 260 82 696.4 0 82 261 93 696.4 0 93 262 238 696.4 0 238 263 248 696.4 0 248 264 263 696.4 0 263 265 80 696.4 0 80 266 103 696.4 0 103 267 700 696.4 0 700 268 756 696.4 0 756 269 748 696.4 0 748 270 331 696.4 0 331 271 41 696.4 0 41 272 97 696.4 0 97 273 155 696.4 0 155 274 284 696.4 0 284 275 243 696.4 0 243 276 151 696.4 0 151 277 118 696.4 0 118 278 61 696.4 0 61 279 69 696.4 0 69 280 82 696.4 0 82 281 141 696.4 0 141 282 107 696.4 0 107 283 108 696.4 0 108 284 51 696.4 0 51 285 36 696.4 0 36 286 58 696.4 0 58 287 78 696.4 0 78 288 116 696.4 0 116 289 104 696.4 0 104 290 95 696.4 0 95 291 73 696.4 0 73 292 51 696.4 0 51 293 83 696.4 0 83 294 68 696.4 0 68 295 95 696.4 0 95 296 70 696.4 0 70 297 65 696.4 0 65 298 41 696.4 0 41 299 61 696.4 0 61 300 82 696.4 0 82 301 50 696.4 0 50 Finished in 8.79 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 186,853 Reads with adapters: 88,082 (47.1%) Reads that were too short: 153 (0.1%) Reads written (passing filters): 87,929 (47.1%) Total basepairs processed: 52,986,631 bp Total written (filtered): 24,330,603 bp (45.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 88082 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 42 0.0 3 0 0 15 27 16 840 0.0 3 0 729 74 37 17 86224 0.0 3 81233 4171 658 162 18 170 0.0 3 6 146 12 6 19 190 0.0 3 3 0 106 81 20 282 0.0 3 1 0 0 281 21 2 0.0 3 1 0 0 1 25 2 0.0 3 2 26 2 0.0 3 2 28 1 0.0 3 0 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 21 0.0 3 0 0 0 21 45 8 0.0 3 0 0 0 8 48 6 0.0 3 0 0 0 6 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 51 4 0.0 3 0 0 0 4 52 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 61 4 0.0 3 0 0 0 4 62 1 0.0 3 0 0 0 1 63 39 0.0 3 0 0 1 38 64 3 0.0 3 0 0 0 3 65 18 0.0 3 0 0 0 18 66 12 0.0 3 0 0 0 12 67 1 0.0 3 0 0 0 1 68 5 0.0 3 0 0 0 5 69 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 3 0.0 3 0 0 0 3 116 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 137 7 0.0 3 0 0 0 7 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 9 0.0 3 0 0 0 9 165 1 0.0 3 0 0 0 1 166 4 0.0 3 0 0 0 4 167 2 0.0 3 0 0 0 2 172 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 194 8 0.0 3 0 0 0 8 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 205 10 0.0 3 0 0 1 9 206 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 21 0.0 3 0 0 1 20 218 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 271 2 0.0 3 0 0 0 2 278 2 0.0 3 0 0 0 2 279 9 0.0 3 0 0 0 9 280 8 0.0 3 0 0 1 7 281 14 0.0 3 0 0 0 14 282 8 0.0 3 0 0 0 8 283 3 0.0 3 0 0 0 3 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.09 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 87,929 Reads with adapters: 757 (0.9%) Reads written (passing filters): 87,929 (100.0%) Total basepairs processed: 24,330,603 bp Total written (filtered): 24,328,252 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 757 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.1% C: 59.7% G: 15.5% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 709 1373.9 0 709 4 26 343.5 0 26 5 15 85.9 0 15 6 6 21.5 0 6 9 1 0.3 0 0 1