This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGTGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.11 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 200,098 (7.0%) Reads that were too short: 9,597 (0.3%) Reads written (passing filters): 190,501 (6.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 54,373,671 bp (6.3%) === Adapter 1 === Sequence: AAGTGT; Type: regular 5'; Length: 6; Trimmed: 200098 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4533 696.4 0 4533 7 85209 696.4 0 85209 8 87567 696.4 0 87567 9 113 696.4 0 113 10 29 696.4 0 29 11 22 696.4 0 22 12 31 696.4 0 31 13 26 696.4 0 26 14 26 696.4 0 26 15 35 696.4 0 35 16 34 696.4 0 34 17 22 696.4 0 22 18 42 696.4 0 42 19 34 696.4 0 34 20 29 696.4 0 29 21 38 696.4 0 38 22 26 696.4 0 26 23 23 696.4 0 23 24 20 696.4 0 20 25 16 696.4 0 16 26 16 696.4 0 16 27 24 696.4 0 24 28 17 696.4 0 17 29 22 696.4 0 22 30 22 696.4 0 22 31 22 696.4 0 22 32 20 696.4 0 20 33 45 696.4 0 45 34 30 696.4 0 30 35 26 696.4 0 26 36 27 696.4 0 27 37 36 696.4 0 36 38 22 696.4 0 22 39 31 696.4 0 31 40 63 696.4 0 63 41 44 696.4 0 44 42 21 696.4 0 21 43 25 696.4 0 25 44 33 696.4 0 33 45 45 696.4 0 45 46 52 696.4 0 52 47 34 696.4 0 34 48 33 696.4 0 33 49 26 696.4 0 26 50 27 696.4 0 27 51 31 696.4 0 31 52 51 696.4 0 51 53 28 696.4 0 28 54 82 696.4 0 82 55 410 696.4 0 410 56 65 696.4 0 65 57 22 696.4 0 22 58 18 696.4 0 18 59 46 696.4 0 46 60 35 696.4 0 35 61 158 696.4 0 158 62 164 696.4 0 164 63 198 696.4 0 198 64 32 696.4 0 32 65 56 696.4 0 56 66 36 696.4 0 36 67 85 696.4 0 85 68 58 696.4 0 58 69 87 696.4 0 87 70 54 696.4 0 54 71 22 696.4 0 22 72 25 696.4 0 25 73 26 696.4 0 26 74 34 696.4 0 34 75 29 696.4 0 29 76 58 696.4 0 58 77 40 696.4 0 40 78 63 696.4 0 63 79 46 696.4 0 46 80 68 696.4 0 68 81 38 696.4 0 38 82 28 696.4 0 28 83 49 696.4 0 49 84 38 696.4 0 38 85 38 696.4 0 38 86 36 696.4 0 36 87 63 696.4 0 63 88 59 696.4 0 59 89 51 696.4 0 51 90 51 696.4 0 51 91 56 696.4 0 56 92 40 696.4 0 40 93 25 696.4 0 25 94 37 696.4 0 37 95 50 696.4 0 50 96 60 696.4 0 60 97 64 696.4 0 64 98 81 696.4 0 81 99 43 696.4 0 43 100 31 696.4 0 31 101 43 696.4 0 43 102 30 696.4 0 30 103 27 696.4 0 27 104 38 696.4 0 38 105 43 696.4 0 43 106 20 696.4 0 20 107 38 696.4 0 38 108 38 696.4 0 38 109 29 696.4 0 29 110 28 696.4 0 28 111 36 696.4 0 36 112 54 696.4 0 54 113 49 696.4 0 49 114 38 696.4 0 38 115 56 696.4 0 56 116 36 696.4 0 36 117 30 696.4 0 30 118 31 696.4 0 31 119 41 696.4 0 41 120 42 696.4 0 42 121 54 696.4 0 54 122 53 696.4 0 53 123 123 696.4 0 123 124 78 696.4 0 78 125 114 696.4 0 114 126 255 696.4 0 255 127 168 696.4 0 168 128 429 696.4 0 429 129 331 696.4 0 331 130 351 696.4 0 351 131 173 696.4 0 173 132 110 696.4 0 110 133 43 696.4 0 43 134 88 696.4 0 88 135 77 696.4 0 77 136 97 696.4 0 97 137 42 696.4 0 42 138 51 696.4 0 51 139 42 696.4 0 42 140 404 696.4 0 404 141 47 696.4 0 47 142 29 696.4 0 29 143 93 696.4 0 93 144 35 696.4 0 35 145 63 696.4 0 63 146 39 696.4 0 39 147 48 696.4 0 48 148 49 696.4 0 49 149 59 696.4 0 59 150 44 696.4 0 44 151 57 696.4 0 57 152 36 696.4 0 36 153 20 696.4 0 20 154 37 696.4 0 37 155 44 696.4 0 44 156 41 696.4 0 41 157 24 696.4 0 24 158 33 696.4 0 33 159 45 696.4 0 45 160 40 696.4 0 40 161 30 696.4 0 30 162 35 696.4 0 35 163 39 696.4 0 39 164 51 696.4 0 51 165 56 696.4 0 56 166 163 696.4 0 163 167 135 696.4 0 135 168 118 696.4 0 118 169 35 696.4 0 35 170 33 696.4 0 33 171 61 696.4 0 61 172 47 696.4 0 47 173 39 696.4 0 39 174 68 696.4 0 68 175 55 696.4 0 55 176 58 696.4 0 58 177 45 696.4 0 45 178 37 696.4 0 37 179 40 696.4 0 40 180 43 696.4 0 43 181 31 696.4 0 31 182 42 696.4 0 42 183 77 696.4 0 77 184 77 696.4 0 77 185 69 696.4 0 69 186 49 696.4 0 49 187 55 696.4 0 55 188 40 696.4 0 40 189 52 696.4 0 52 190 36 696.4 0 36 191 46 696.4 0 46 192 77 696.4 0 77 193 61 696.4 0 61 194 71 696.4 0 71 195 80 696.4 0 80 196 237 696.4 0 237 197 547 696.4 0 547 198 532 696.4 0 532 199 574 696.4 0 574 200 87 696.4 0 87 201 57 696.4 0 57 202 62 696.4 0 62 203 43 696.4 0 43 204 58 696.4 0 58 205 48 696.4 0 48 206 49 696.4 0 49 207 51 696.4 0 51 208 62 696.4 0 62 209 47 696.4 0 47 210 52 696.4 0 52 211 91 696.4 0 91 212 70 696.4 0 70 213 79 696.4 0 79 214 60 696.4 0 60 215 70 696.4 0 70 216 54 696.4 0 54 217 141 696.4 0 141 218 123 696.4 0 123 219 146 696.4 0 146 220 279 696.4 0 279 221 153 696.4 0 153 222 184 696.4 0 184 223 79 696.4 0 79 224 63 696.4 0 63 225 62 696.4 0 62 226 66 696.4 0 66 227 54 696.4 0 54 228 61 696.4 0 61 229 46 696.4 0 46 230 46 696.4 0 46 231 45 696.4 0 45 232 52 696.4 0 52 233 68 696.4 0 68 234 55 696.4 0 55 235 59 696.4 0 59 236 74 696.4 0 74 237 52 696.4 0 52 238 147 696.4 0 147 239 135 696.4 0 135 240 124 696.4 0 124 241 108 696.4 0 108 242 141 696.4 0 141 243 65 696.4 0 65 244 74 696.4 0 74 245 104 696.4 0 104 246 136 696.4 0 136 247 115 696.4 0 115 248 104 696.4 0 104 249 167 696.4 0 167 250 70 696.4 0 70 251 105 696.4 0 105 252 54 696.4 0 54 253 59 696.4 0 59 254 320 696.4 0 320 255 257 696.4 0 257 256 168 696.4 0 168 257 72 696.4 0 72 258 71 696.4 0 71 259 57 696.4 0 57 260 47 696.4 0 47 261 52 696.4 0 52 262 303 696.4 0 303 263 322 696.4 0 322 264 443 696.4 0 443 265 54 696.4 0 54 266 56 696.4 0 56 267 252 696.4 0 252 268 292 696.4 0 292 269 287 696.4 0 287 270 158 696.4 0 158 271 33 696.4 0 33 272 54 696.4 0 54 273 108 696.4 0 108 274 136 696.4 0 136 275 130 696.4 0 130 276 83 696.4 0 83 277 60 696.4 0 60 278 39 696.4 0 39 279 44 696.4 0 44 280 55 696.4 0 55 281 84 696.4 0 84 282 83 696.4 0 83 283 59 696.4 0 59 284 41 696.4 0 41 285 29 696.4 0 29 286 39 696.4 0 39 287 40 696.4 0 40 288 85 696.4 0 85 289 64 696.4 0 64 290 49 696.4 0 49 291 52 696.4 0 52 292 39 696.4 0 39 293 60 696.4 0 60 294 54 696.4 0 54 295 68 696.4 0 68 296 45 696.4 0 45 297 46 696.4 0 46 298 38 696.4 0 38 299 46 696.4 0 46 300 51 696.4 0 51 301 60 696.4 0 60 Finished in 9.36 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 190,501 Reads with adapters: 95,574 (50.2%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 95,526 (50.1%) Total basepairs processed: 54,373,671 bp Total written (filtered): 26,033,286 bp (47.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 95574 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 29 0.0 3 0 0 27 1 1 19 981 0.0 3 0 952 24 4 1 20 90276 0.0 4 88705 1509 54 4 4 21 225 0.0 4 33 182 7 3 22 3446 0.0 4 3364 55 22 1 4 23 89 0.0 4 4 5 1 74 5 24 7 0.0 4 1 0 2 2 2 25 2 0.0 4 0 0 0 2 27 1 0.0 4 1 29 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 1 41 1 0.0 4 1 44 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 448 0.0 4 0 0 0 0 448 128 2 0.0 4 0 0 0 0 2 139 7 0.0 4 0 0 0 0 7 147 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 1 177 1 0.0 4 0 0 0 0 1 196 1 0.0 4 1 222 2 0.0 4 0 0 0 0 2 228 13 0.0 4 0 0 0 0 13 229 4 0.0 4 0 0 0 0 4 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 294 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.38 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 95,526 Reads with adapters: 6,651 (7.0%) Reads written (passing filters): 95,526 (100.0%) Total basepairs processed: 26,033,286 bp Total written (filtered): 25,955,337 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6651 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 3.6% G: 92.1% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 72 1492.6 0 72 4 15 373.1 0 15 5 6 93.3 0 6 6 1 23.3 0 1 7 1 5.8 0 1 8 5 1.5 0 5 9 275 0.4 0 4 271 10 2051 0.1 1 62 1989 11 4161 0.0 1 28 4133 12 16 0.0 1 0 16 13 2 0.0 1 0 2 14 1 0.0 1 0 1 15 1 0.0 1 0 1 28 1 0.0 1 1 106 1 0.0 1 0 1 107 5 0.0 1 4 1 108 3 0.0 1 2 1 109 2 0.0 1 1 1 151 4 0.0 1 0 4 190 1 0.0 1 0 1 209 5 0.0 1 3 2 224 5 0.0 1 3 2 258 1 0.0 1 1 259 12 0.0 1 10 2 260 1 0.0 1 1 263 2 0.0 1 1 1 265 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.