This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 77.53 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 113,999 (4.0%) Reads that were too short: 14,532 (0.5%) Reads written (passing filters): 99,467 (3.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 27,520,126 bp (3.2%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 113999 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1294 696.4 0 1294 7 47193 696.4 0 47193 8 667 696.4 0 667 9 36922 696.4 0 36922 10 31 696.4 0 31 11 16 696.4 0 16 12 8 696.4 0 8 13 10 696.4 0 10 14 5 696.4 0 5 15 272 696.4 0 272 16 35 696.4 0 35 17 12 696.4 0 12 18 10 696.4 0 10 19 5 696.4 0 5 20 8 696.4 0 8 21 9 696.4 0 9 22 12 696.4 0 12 23 22 696.4 0 22 24 11 696.4 0 11 25 9 696.4 0 9 26 7 696.4 0 7 27 7 696.4 0 7 28 3 696.4 0 3 29 9 696.4 0 9 30 15 696.4 0 15 31 10 696.4 0 10 32 12 696.4 0 12 33 25 696.4 0 25 34 12 696.4 0 12 35 17 696.4 0 17 36 15 696.4 0 15 37 8 696.4 0 8 38 23 696.4 0 23 39 23 696.4 0 23 40 10 696.4 0 10 41 216 696.4 0 216 42 36 696.4 0 36 43 20 696.4 0 20 44 15 696.4 0 15 45 23 696.4 0 23 46 16 696.4 0 16 47 17 696.4 0 17 48 7 696.4 0 7 49 11 696.4 0 11 50 15 696.4 0 15 51 17 696.4 0 17 52 18 696.4 0 18 53 6 696.4 0 6 54 7 696.4 0 7 55 8 696.4 0 8 56 14 696.4 0 14 57 16 696.4 0 16 58 9 696.4 0 9 59 14 696.4 0 14 60 10 696.4 0 10 61 14 696.4 0 14 62 5 696.4 0 5 63 11 696.4 0 11 64 11 696.4 0 11 65 11 696.4 0 11 66 15 696.4 0 15 67 16 696.4 0 16 68 11 696.4 0 11 69 92 696.4 0 92 70 113 696.4 0 113 71 91 696.4 0 91 72 14 696.4 0 14 73 16 696.4 0 16 74 13 696.4 0 13 75 7 696.4 0 7 76 23 696.4 0 23 77 16 696.4 0 16 78 15 696.4 0 15 79 19 696.4 0 19 80 28 696.4 0 28 81 28 696.4 0 28 82 35 696.4 0 35 83 33 696.4 0 33 84 33 696.4 0 33 85 44 696.4 0 44 86 44 696.4 0 44 87 109 696.4 0 109 88 47 696.4 0 47 89 76 696.4 0 76 90 87 696.4 0 87 91 68 696.4 0 68 92 49 696.4 0 49 93 45 696.4 0 45 94 36 696.4 0 36 95 39 696.4 0 39 96 22 696.4 0 22 97 52 696.4 0 52 98 49 696.4 0 49 99 45 696.4 0 45 100 304 696.4 0 304 101 308 696.4 0 308 102 1328 696.4 0 1328 103 175 696.4 0 175 104 52 696.4 0 52 105 35 696.4 0 35 106 23 696.4 0 23 107 41 696.4 0 41 108 23 696.4 0 23 109 27 696.4 0 27 110 35 696.4 0 35 111 41 696.4 0 41 112 31 696.4 0 31 113 22 696.4 0 22 114 21 696.4 0 21 115 33 696.4 0 33 116 38 696.4 0 38 117 35 696.4 0 35 118 71 696.4 0 71 119 32 696.4 0 32 120 37 696.4 0 37 121 30 696.4 0 30 122 63 696.4 0 63 123 80 696.4 0 80 124 84 696.4 0 84 125 53 696.4 0 53 126 50 696.4 0 50 127 26 696.4 0 26 128 33 696.4 0 33 129 31 696.4 0 31 130 47 696.4 0 47 131 195 696.4 0 195 132 159 696.4 0 159 133 108 696.4 0 108 134 66 696.4 0 66 135 37 696.4 0 37 136 42 696.4 0 42 137 70 696.4 0 70 138 69 696.4 0 69 139 66 696.4 0 66 140 76 696.4 0 76 141 57 696.4 0 57 142 122 696.4 0 122 143 595 696.4 0 595 144 143 696.4 0 143 145 185 696.4 0 185 146 66 696.4 0 66 147 53 696.4 0 53 148 57 696.4 0 57 149 60 696.4 0 60 150 50 696.4 0 50 151 64 696.4 0 64 152 63 696.4 0 63 153 57 696.4 0 57 154 57 696.4 0 57 155 51 696.4 0 51 156 54 696.4 0 54 157 116 696.4 0 116 158 184 696.4 0 184 159 164 696.4 0 164 160 282 696.4 0 282 161 233 696.4 0 233 162 194 696.4 0 194 163 59 696.4 0 59 164 63 696.4 0 63 165 45 696.4 0 45 166 36 696.4 0 36 167 54 696.4 0 54 168 62 696.4 0 62 169 82 696.4 0 82 170 73 696.4 0 73 171 69 696.4 0 69 172 51 696.4 0 51 173 57 696.4 0 57 174 44 696.4 0 44 175 67 696.4 0 67 176 398 696.4 0 398 177 404 696.4 0 404 178 338 696.4 0 338 179 73 696.4 0 73 180 64 696.4 0 64 181 70 696.4 0 70 182 45 696.4 0 45 183 90 696.4 0 90 184 106 696.4 0 106 185 75 696.4 0 75 186 58 696.4 0 58 187 92 696.4 0 92 188 153 696.4 0 153 189 166 696.4 0 166 190 90 696.4 0 90 191 55 696.4 0 55 192 43 696.4 0 43 193 30 696.4 0 30 194 59 696.4 0 59 195 52 696.4 0 52 196 77 696.4 0 77 197 99 696.4 0 99 198 140 696.4 0 140 199 81 696.4 0 81 200 69 696.4 0 69 201 50 696.4 0 50 202 69 696.4 0 69 203 67 696.4 0 67 204 98 696.4 0 98 205 60 696.4 0 60 206 68 696.4 0 68 207 55 696.4 0 55 208 32 696.4 0 32 209 49 696.4 0 49 210 110 696.4 0 110 211 142 696.4 0 142 212 160 696.4 0 160 213 144 696.4 0 144 214 71 696.4 0 71 215 86 696.4 0 86 216 82 696.4 0 82 217 89 696.4 0 89 218 91 696.4 0 91 219 66 696.4 0 66 220 75 696.4 0 75 221 83 696.4 0 83 222 98 696.4 0 98 223 118 696.4 0 118 224 242 696.4 0 242 225 171 696.4 0 171 226 124 696.4 0 124 227 78 696.4 0 78 228 102 696.4 0 102 229 70 696.4 0 70 230 87 696.4 0 87 231 67 696.4 0 67 232 72 696.4 0 72 233 70 696.4 0 70 234 82 696.4 0 82 235 90 696.4 0 90 236 100 696.4 0 100 237 109 696.4 0 109 238 268 696.4 0 268 239 304 696.4 0 304 240 164 696.4 0 164 241 98 696.4 0 98 242 107 696.4 0 107 243 131 696.4 0 131 244 137 696.4 0 137 245 143 696.4 0 143 246 131 696.4 0 131 247 124 696.4 0 124 248 114 696.4 0 114 249 111 696.4 0 111 250 104 696.4 0 104 251 144 696.4 0 144 252 101 696.4 0 101 253 105 696.4 0 105 254 104 696.4 0 104 255 108 696.4 0 108 256 155 696.4 0 155 257 127 696.4 0 127 258 128 696.4 0 128 259 124 696.4 0 124 260 140 696.4 0 140 261 104 696.4 0 104 262 459 696.4 0 459 263 514 696.4 0 514 264 965 696.4 0 965 265 306 696.4 0 306 266 221 696.4 0 221 267 109 696.4 0 109 268 85 696.4 0 85 269 88 696.4 0 88 270 96 696.4 0 96 271 180 696.4 0 180 272 162 696.4 0 162 273 158 696.4 0 158 274 279 696.4 0 279 275 174 696.4 0 174 276 195 696.4 0 195 277 146 696.4 0 146 278 146 696.4 0 146 279 118 696.4 0 118 280 100 696.4 0 100 281 114 696.4 0 114 282 137 696.4 0 137 283 171 696.4 0 171 284 183 696.4 0 183 285 160 696.4 0 160 286 134 696.4 0 134 287 138 696.4 0 138 288 138 696.4 0 138 289 93 696.4 0 93 290 104 696.4 0 104 291 152 696.4 0 152 292 215 696.4 0 215 293 161 696.4 0 161 294 144 696.4 0 144 295 132 696.4 0 132 296 92 696.4 0 92 297 121 696.4 0 121 298 169 696.4 0 169 299 239 696.4 0 239 300 217 696.4 0 217 301 364 696.4 0 364 Finished in 5.00 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 99,467 Reads with adapters: 48,439 (48.7%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 48,421 (48.7%) Total basepairs processed: 27,520,126 bp Total written (filtered): 13,206,727 bp (48.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48439 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 16 0.0 3 0 0 15 1 19 382 0.0 3 0 372 10 20 46997 0.0 4 46204 762 29 2 21 115 0.0 4 11 102 1 0 1 22 9 0.0 4 0 4 5 23 346 0.0 4 304 4 0 32 6 24 2 0.0 4 0 1 0 0 1 26 2 0.0 4 0 1 0 0 1 28 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 1 125 3 0.0 4 0 0 0 0 3 126 22 0.0 4 0 0 0 0 22 127 514 0.0 4 0 0 0 0 514 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 134 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.67 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 48,421 Reads with adapters: 1,273 (2.6%) Reads written (passing filters): 48,421 (100.0%) Total basepairs processed: 13,206,727 bp Total written (filtered): 13,173,089 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1273 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 43.8% C: 4.2% G: 46.2% T: 5.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 756.6 0 52 4 9 189.1 0 9 5 3 47.3 0 3 6 3 11.8 0 3 8 3 0.7 0 3 9 51 0.2 0 1 50 10 21 0.0 1 2 19 11 512 0.0 1 8 504 12 38 0.0 1 1 37 13 3 0.0 1 0 3 15 3 0.0 1 0 3 18 1 0.0 1 0 1 19 3 0.0 1 1 2 20 428 0.0 1 334 94 21 23 0.0 1 15 8 23 1 0.0 1 0 1 24 2 0.0 1 2 25 36 0.0 1 30 6 26 1 0.0 1 1 28 3 0.0 1 3 124 1 0.0 1 0 1 131 2 0.0 1 2 152 2 0.0 1 0 2 158 4 0.0 1 3 1 191 2 0.0 1 0 2 210 16 0.0 1 3 13 225 49 0.0 1 45 4 260 1 0.0 1 1