This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.76 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 169,953 (6.0%) Reads that were too short: 31,017 (1.1%) Reads written (passing filters): 138,936 (4.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,286,746 bp (4.6%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 169953 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2865 696.4 0 2865 7 1980 696.4 0 1980 8 65611 696.4 0 65611 9 55603 696.4 0 55603 10 107 696.4 0 107 11 43 696.4 0 43 12 109 696.4 0 109 13 71 696.4 0 71 14 41 696.4 0 41 15 37 696.4 0 37 16 33 696.4 0 33 17 60 696.4 0 60 18 34 696.4 0 34 19 63 696.4 0 63 20 44 696.4 0 44 21 35 696.4 0 35 22 37 696.4 0 37 23 68 696.4 0 68 24 41 696.4 0 41 25 32 696.4 0 32 26 37 696.4 0 37 27 27 696.4 0 27 28 33 696.4 0 33 29 37 696.4 0 37 30 26 696.4 0 26 31 42 696.4 0 42 32 39 696.4 0 39 33 36 696.4 0 36 34 45 696.4 0 45 35 39 696.4 0 39 36 35 696.4 0 35 37 43 696.4 0 43 38 36 696.4 0 36 39 48 696.4 0 48 40 71 696.4 0 71 41 52 696.4 0 52 42 47 696.4 0 47 43 35 696.4 0 35 44 42 696.4 0 42 45 39 696.4 0 39 46 44 696.4 0 44 47 54 696.4 0 54 48 40 696.4 0 40 49 32 696.4 0 32 50 34 696.4 0 34 51 78 696.4 0 78 52 87 696.4 0 87 53 178 696.4 0 178 54 57 696.4 0 57 55 36 696.4 0 36 56 27 696.4 0 27 57 24 696.4 0 24 58 46 696.4 0 46 59 39 696.4 0 39 60 30 696.4 0 30 61 45 696.4 0 45 62 48 696.4 0 48 63 34 696.4 0 34 64 71 696.4 0 71 65 36 696.4 0 36 66 43 696.4 0 43 67 48 696.4 0 48 68 60 696.4 0 60 69 37 696.4 0 37 70 34 696.4 0 34 71 45 696.4 0 45 72 44 696.4 0 44 73 30 696.4 0 30 74 50 696.4 0 50 75 57 696.4 0 57 76 70 696.4 0 70 77 57 696.4 0 57 78 45 696.4 0 45 79 62 696.4 0 62 80 50 696.4 0 50 81 57 696.4 0 57 82 61 696.4 0 61 83 61 696.4 0 61 84 41 696.4 0 41 85 67 696.4 0 67 86 65 696.4 0 65 87 82 696.4 0 82 88 41 696.4 0 41 89 60 696.4 0 60 90 60 696.4 0 60 91 60 696.4 0 60 92 63 696.4 0 63 93 126 696.4 0 126 94 70 696.4 0 70 95 44 696.4 0 44 96 56 696.4 0 56 97 65 696.4 0 65 98 39 696.4 0 39 99 94 696.4 0 94 100 120 696.4 0 120 101 115 696.4 0 115 102 49 696.4 0 49 103 50 696.4 0 50 104 36 696.4 0 36 105 46 696.4 0 46 106 41 696.4 0 41 107 50 696.4 0 50 108 33 696.4 0 33 109 56 696.4 0 56 110 41 696.4 0 41 111 48 696.4 0 48 112 51 696.4 0 51 113 52 696.4 0 52 114 47 696.4 0 47 115 41 696.4 0 41 116 72 696.4 0 72 117 90 696.4 0 90 118 98 696.4 0 98 119 49 696.4 0 49 120 57 696.4 0 57 121 65 696.4 0 65 122 52 696.4 0 52 123 43 696.4 0 43 124 43 696.4 0 43 125 57 696.4 0 57 126 62 696.4 0 62 127 53 696.4 0 53 128 48 696.4 0 48 129 47 696.4 0 47 130 52 696.4 0 52 131 75 696.4 0 75 132 94 696.4 0 94 133 81 696.4 0 81 134 43 696.4 0 43 135 67 696.4 0 67 136 81 696.4 0 81 137 677 696.4 0 677 138 718 696.4 0 718 139 695 696.4 0 695 140 318 696.4 0 318 141 41 696.4 0 41 142 73 696.4 0 73 143 65 696.4 0 65 144 97 696.4 0 97 145 73 696.4 0 73 146 60 696.4 0 60 147 50 696.4 0 50 148 53 696.4 0 53 149 49 696.4 0 49 150 45 696.4 0 45 151 51 696.4 0 51 152 56 696.4 0 56 153 68 696.4 0 68 154 59 696.4 0 59 155 48 696.4 0 48 156 50 696.4 0 50 157 67 696.4 0 67 158 63 696.4 0 63 159 57 696.4 0 57 160 54 696.4 0 54 161 46 696.4 0 46 162 46 696.4 0 46 163 43 696.4 0 43 164 52 696.4 0 52 165 66 696.4 0 66 166 52 696.4 0 52 167 40 696.4 0 40 168 49 696.4 0 49 169 71 696.4 0 71 170 56 696.4 0 56 171 64 696.4 0 64 172 59 696.4 0 59 173 62 696.4 0 62 174 54 696.4 0 54 175 33 696.4 0 33 176 60 696.4 0 60 177 43 696.4 0 43 178 53 696.4 0 53 179 103 696.4 0 103 180 95 696.4 0 95 181 51 696.4 0 51 182 61 696.4 0 61 183 62 696.4 0 62 184 60 696.4 0 60 185 60 696.4 0 60 186 68 696.4 0 68 187 98 696.4 0 98 188 84 696.4 0 84 189 103 696.4 0 103 190 48 696.4 0 48 191 59 696.4 0 59 192 47 696.4 0 47 193 45 696.4 0 45 194 56 696.4 0 56 195 39 696.4 0 39 196 52 696.4 0 52 197 55 696.4 0 55 198 74 696.4 0 74 199 69 696.4 0 69 200 41 696.4 0 41 201 58 696.4 0 58 202 64 696.4 0 64 203 75 696.4 0 75 204 76 696.4 0 76 205 61 696.4 0 61 206 79 696.4 0 79 207 62 696.4 0 62 208 74 696.4 0 74 209 83 696.4 0 83 210 109 696.4 0 109 211 79 696.4 0 79 212 67 696.4 0 67 213 57 696.4 0 57 214 231 696.4 0 231 215 115 696.4 0 115 216 216 696.4 0 216 217 47 696.4 0 47 218 74 696.4 0 74 219 47 696.4 0 47 220 62 696.4 0 62 221 42 696.4 0 42 222 50 696.4 0 50 223 63 696.4 0 63 224 65 696.4 0 65 225 58 696.4 0 58 226 66 696.4 0 66 227 53 696.4 0 53 228 75 696.4 0 75 229 62 696.4 0 62 230 46 696.4 0 46 231 64 696.4 0 64 232 58 696.4 0 58 233 67 696.4 0 67 234 63 696.4 0 63 235 43 696.4 0 43 236 48 696.4 0 48 237 64 696.4 0 64 238 132 696.4 0 132 239 71 696.4 0 71 240 101 696.4 0 101 241 59 696.4 0 59 242 59 696.4 0 59 243 147 696.4 0 147 244 93 696.4 0 93 245 153 696.4 0 153 246 82 696.4 0 82 247 80 696.4 0 80 248 65 696.4 0 65 249 70 696.4 0 70 250 51 696.4 0 51 251 61 696.4 0 61 252 73 696.4 0 73 253 72 696.4 0 72 254 70 696.4 0 70 255 83 696.4 0 83 256 165 696.4 0 165 257 370 696.4 0 370 258 411 696.4 0 411 259 475 696.4 0 475 260 250 696.4 0 250 261 212 696.4 0 212 262 88 696.4 0 88 263 136 696.4 0 136 264 109 696.4 0 109 265 80 696.4 0 80 266 110 696.4 0 110 267 71 696.4 0 71 268 73 696.4 0 73 269 66 696.4 0 66 270 100 696.4 0 100 271 129 696.4 0 129 272 253 696.4 0 253 273 1296 696.4 0 1296 274 980 696.4 0 980 275 1295 696.4 0 1295 276 244 696.4 0 244 277 303 696.4 0 303 278 292 696.4 0 292 279 174 696.4 0 174 280 195 696.4 0 195 281 84 696.4 0 84 282 101 696.4 0 101 283 526 696.4 0 526 284 313 696.4 0 313 285 597 696.4 0 597 286 636 696.4 0 636 287 758 696.4 0 758 288 567 696.4 0 567 289 202 696.4 0 202 290 133 696.4 0 133 291 155 696.4 0 155 292 288 696.4 0 288 293 732 696.4 0 732 294 783 696.4 0 783 295 596 696.4 0 596 296 310 696.4 0 310 297 756 696.4 0 756 298 1879 696.4 0 1879 299 4441 696.4 0 4441 300 3841 696.4 0 3841 301 1255 696.4 0 1255 Finished in 6.50 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 138,936 Reads with adapters: 65,898 (47.4%) Reads that were too short: 117 (0.1%) Reads written (passing filters): 65,781 (47.3%) Total basepairs processed: 39,286,746 bp Total written (filtered): 18,143,141 bp (46.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 65898 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 41 0.0 3 0 0 23 18 16 580 0.0 3 0 472 78 30 17 64440 0.0 3 60623 3123 554 140 18 188 0.0 3 13 152 16 7 19 135 0.0 3 14 2 70 49 20 297 0.0 3 4 0 1 292 21 1 0.0 3 1 23 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 3 0.0 3 1 0 1 1 26 1 0.0 3 0 0 0 1 29 3 0.0 3 1 0 0 2 33 2 0.0 3 0 0 0 2 35 1 0.0 3 1 38 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 1 1 42 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 28 0.0 3 0 0 0 28 56 1 0.0 3 0 0 0 1 58 8 0.0 3 0 0 0 8 73 1 0.0 3 0 0 0 1 74 1 0.0 3 1 75 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 1 98 2 0.0 3 0 0 1 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 173 1 0.0 3 1 174 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 189 3 0.0 3 0 0 0 3 192 5 0.0 3 0 0 0 5 194 6 0.0 3 0 0 0 6 195 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 217 29 0.0 3 0 0 1 28 218 1 0.0 3 0 0 0 1 219 10 0.0 3 0 0 0 10 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 279 8 0.0 3 0 0 0 8 280 1 0.0 3 0 0 0 1 281 9 0.0 3 0 0 0 9 282 14 0.0 3 0 0 0 14 285 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.30 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 65,781 Reads with adapters: 265 (0.4%) Reads written (passing filters): 65,781 (100.0%) Total basepairs processed: 18,143,141 bp Total written (filtered): 18,142,246 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 265 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.2% C: 14.3% G: 32.8% T: 16.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 194 1027.8 0 194 4 50 257.0 0 50 5 17 64.2 0 17 6 3 16.1 0 3 10 1 0.1 1 0 1