This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.44 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 171,906 (6.0%) Reads that were too short: 32,512 (1.1%) Reads written (passing filters): 139,394 (4.9%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,838,191 bp (4.6%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 171906 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2617 696.4 0 2617 7 1492 696.4 0 1492 8 60032 696.4 0 60032 9 66228 696.4 0 66228 10 84 696.4 0 84 11 31 696.4 0 31 12 43 696.4 0 43 13 58 696.4 0 58 14 34 696.4 0 34 15 25 696.4 0 25 16 21 696.4 0 21 17 39 696.4 0 39 18 21 696.4 0 21 19 37 696.4 0 37 20 35 696.4 0 35 21 34 696.4 0 34 22 34 696.4 0 34 23 30 696.4 0 30 24 50 696.4 0 50 25 39 696.4 0 39 26 28 696.4 0 28 27 34 696.4 0 34 28 26 696.4 0 26 29 30 696.4 0 30 30 35 696.4 0 35 31 37 696.4 0 37 32 26 696.4 0 26 33 36 696.4 0 36 34 30 696.4 0 30 35 35 696.4 0 35 36 40 696.4 0 40 37 27 696.4 0 27 38 44 696.4 0 44 39 28 696.4 0 28 40 179 696.4 0 179 41 40 696.4 0 40 42 42 696.4 0 42 43 24 696.4 0 24 44 41 696.4 0 41 45 33 696.4 0 33 46 44 696.4 0 44 47 42 696.4 0 42 48 29 696.4 0 29 49 27 696.4 0 27 50 38 696.4 0 38 51 39 696.4 0 39 52 38 696.4 0 38 53 63 696.4 0 63 54 40 696.4 0 40 55 41 696.4 0 41 56 28 696.4 0 28 57 22 696.4 0 22 58 26 696.4 0 26 59 41 696.4 0 41 60 24 696.4 0 24 61 31 696.4 0 31 62 39 696.4 0 39 63 27 696.4 0 27 64 45 696.4 0 45 65 45 696.4 0 45 66 60 696.4 0 60 67 43 696.4 0 43 68 30 696.4 0 30 69 37 696.4 0 37 70 28 696.4 0 28 71 30 696.4 0 30 72 32 696.4 0 32 73 39 696.4 0 39 74 28 696.4 0 28 75 37 696.4 0 37 76 36 696.4 0 36 77 40 696.4 0 40 78 40 696.4 0 40 79 38 696.4 0 38 80 29 696.4 0 29 81 41 696.4 0 41 82 34 696.4 0 34 83 47 696.4 0 47 84 34 696.4 0 34 85 42 696.4 0 42 86 33 696.4 0 33 87 35 696.4 0 35 88 38 696.4 0 38 89 34 696.4 0 34 90 40 696.4 0 40 91 38 696.4 0 38 92 53 696.4 0 53 93 58 696.4 0 58 94 53 696.4 0 53 95 40 696.4 0 40 96 35 696.4 0 35 97 34 696.4 0 34 98 47 696.4 0 47 99 36 696.4 0 36 100 68 696.4 0 68 101 67 696.4 0 67 102 47 696.4 0 47 103 41 696.4 0 41 104 28 696.4 0 28 105 32 696.4 0 32 106 42 696.4 0 42 107 52 696.4 0 52 108 34 696.4 0 34 109 49 696.4 0 49 110 50 696.4 0 50 111 33 696.4 0 33 112 36 696.4 0 36 113 42 696.4 0 42 114 28 696.4 0 28 115 33 696.4 0 33 116 49 696.4 0 49 117 46 696.4 0 46 118 46 696.4 0 46 119 49 696.4 0 49 120 32 696.4 0 32 121 44 696.4 0 44 122 48 696.4 0 48 123 34 696.4 0 34 124 24 696.4 0 24 125 53 696.4 0 53 126 35 696.4 0 35 127 33 696.4 0 33 128 37 696.4 0 37 129 41 696.4 0 41 130 44 696.4 0 44 131 61 696.4 0 61 132 48 696.4 0 48 133 50 696.4 0 50 134 38 696.4 0 38 135 45 696.4 0 45 136 63 696.4 0 63 137 243 696.4 0 243 138 397 696.4 0 397 139 421 696.4 0 421 140 183 696.4 0 183 141 39 696.4 0 39 142 35 696.4 0 35 143 41 696.4 0 41 144 51 696.4 0 51 145 44 696.4 0 44 146 44 696.4 0 44 147 41 696.4 0 41 148 42 696.4 0 42 149 38 696.4 0 38 150 42 696.4 0 42 151 35 696.4 0 35 152 39 696.4 0 39 153 31 696.4 0 31 154 37 696.4 0 37 155 40 696.4 0 40 156 43 696.4 0 43 157 45 696.4 0 45 158 48 696.4 0 48 159 38 696.4 0 38 160 32 696.4 0 32 161 30 696.4 0 30 162 34 696.4 0 34 163 31 696.4 0 31 164 39 696.4 0 39 165 39 696.4 0 39 166 37 696.4 0 37 167 33 696.4 0 33 168 45 696.4 0 45 169 38 696.4 0 38 170 42 696.4 0 42 171 46 696.4 0 46 172 47 696.4 0 47 173 49 696.4 0 49 174 31 696.4 0 31 175 28 696.4 0 28 176 47 696.4 0 47 177 60 696.4 0 60 178 61 696.4 0 61 179 89 696.4 0 89 180 91 696.4 0 91 181 42 696.4 0 42 182 43 696.4 0 43 183 52 696.4 0 52 184 54 696.4 0 54 185 48 696.4 0 48 186 55 696.4 0 55 187 53 696.4 0 53 188 45 696.4 0 45 189 59 696.4 0 59 190 25 696.4 0 25 191 32 696.4 0 32 192 52 696.4 0 52 193 45 696.4 0 45 194 40 696.4 0 40 195 47 696.4 0 47 196 39 696.4 0 39 197 46 696.4 0 46 198 61 696.4 0 61 199 51 696.4 0 51 200 40 696.4 0 40 201 50 696.4 0 50 202 34 696.4 0 34 203 50 696.4 0 50 204 43 696.4 0 43 205 43 696.4 0 43 206 52 696.4 0 52 207 39 696.4 0 39 208 38 696.4 0 38 209 47 696.4 0 47 210 49 696.4 0 49 211 44 696.4 0 44 212 40 696.4 0 40 213 56 696.4 0 56 214 85 696.4 0 85 215 71 696.4 0 71 216 87 696.4 0 87 217 46 696.4 0 46 218 54 696.4 0 54 219 47 696.4 0 47 220 54 696.4 0 54 221 32 696.4 0 32 222 37 696.4 0 37 223 49 696.4 0 49 224 43 696.4 0 43 225 38 696.4 0 38 226 52 696.4 0 52 227 57 696.4 0 57 228 62 696.4 0 62 229 48 696.4 0 48 230 42 696.4 0 42 231 52 696.4 0 52 232 36 696.4 0 36 233 38 696.4 0 38 234 70 696.4 0 70 235 43 696.4 0 43 236 40 696.4 0 40 237 43 696.4 0 43 238 69 696.4 0 69 239 41 696.4 0 41 240 82 696.4 0 82 241 38 696.4 0 38 242 55 696.4 0 55 243 58 696.4 0 58 244 63 696.4 0 63 245 56 696.4 0 56 246 51 696.4 0 51 247 55 696.4 0 55 248 62 696.4 0 62 249 52 696.4 0 52 250 42 696.4 0 42 251 64 696.4 0 64 252 63 696.4 0 63 253 29 696.4 0 29 254 44 696.4 0 44 255 35 696.4 0 35 256 64 696.4 0 64 257 110 696.4 0 110 258 117 696.4 0 117 259 118 696.4 0 118 260 101 696.4 0 101 261 93 696.4 0 93 262 69 696.4 0 69 263 87 696.4 0 87 264 57 696.4 0 57 265 56 696.4 0 56 266 105 696.4 0 105 267 65 696.4 0 65 268 63 696.4 0 63 269 60 696.4 0 60 270 78 696.4 0 78 271 99 696.4 0 99 272 182 696.4 0 182 273 1390 696.4 0 1390 274 944 696.4 0 944 275 1492 696.4 0 1492 276 77 696.4 0 77 277 86 696.4 0 86 278 117 696.4 0 117 279 100 696.4 0 100 280 112 696.4 0 112 281 74 696.4 0 74 282 89 696.4 0 89 283 300 696.4 0 300 284 162 696.4 0 162 285 315 696.4 0 315 286 309 696.4 0 309 287 415 696.4 0 415 288 268 696.4 0 268 289 105 696.4 0 105 290 94 696.4 0 94 291 96 696.4 0 96 292 221 696.4 0 221 293 852 696.4 0 852 294 991 696.4 0 991 295 801 696.4 0 801 296 203 696.4 0 203 297 745 696.4 0 745 298 1859 696.4 0 1859 299 5768 696.4 0 5768 300 7070 696.4 0 7070 301 3213 696.4 0 3213 Finished in 7.21 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 139,394 Reads with adapters: 71,004 (50.9%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 70,982 (50.9%) Total basepairs processed: 39,838,191 bp Total written (filtered): 19,257,985 bp (48.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71004 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 32 0.0 3 0 0 30 2 19 710 0.0 3 0 691 14 1 4 20 67855 0.0 4 66497 1292 58 6 2 21 152 0.0 4 26 120 5 1 22 1649 0.0 4 1619 20 9 1 23 54 0.0 4 0 3 1 41 9 24 4 0.0 4 1 0 1 0 2 26 1 0.0 4 1 27 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 64 1 0.0 4 1 74 1 0.0 4 0 1 76 1 0.0 4 1 83 2 0.0 4 0 0 0 1 1 125 2 0.0 4 0 0 0 0 2 126 4 0.0 4 0 0 0 0 4 127 502 0.0 4 0 0 0 0 502 132 2 0.0 4 0 0 0 0 2 136 1 0.0 4 1 137 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 2 0.0 4 1 0 0 0 1 183 1 0.0 4 1 221 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 3 0.0 4 0 0 0 0 3 236 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 1 264 2 0.0 4 0 0 0 0 2 283 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.53 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 70,982 Reads with adapters: 10,356 (14.6%) Reads written (passing filters): 70,982 (100.0%) Total basepairs processed: 19,257,985 bp Total written (filtered): 19,090,628 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 10356 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.5% G: 92.5% T: 3.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 16 1109.1 0 16 4 10 277.3 0 10 5 6 69.3 0 6 6 12 17.3 0 12 7 2 4.3 0 2 8 1 1.1 0 1 9 4771 0.3 0 130 4641 10 4544 0.1 1 150 4394 11 692 0.0 1 7 685 12 8 0.0 1 0 8 13 7 0.0 1 0 7 18 5 0.0 1 3 2 22 11 0.0 1 7 4 150 5 0.0 1 1 4 156 2 0.0 1 2 157 1 0.0 1 1 169 1 0.0 1 0 1 179 1 0.0 1 1 189 1 0.0 1 0 1 191 1 0.0 1 0 1 208 7 0.0 1 0 7 209 1 0.0 1 0 1 210 1 0.0 1 1 223 9 0.0 1 8 1 224 6 0.0 1 6 265 1 0.0 1 1 269 1 0.0 1 1 270 221 0.0 1 211 10 271 7 0.0 1 6 1 272 4 0.0 1 4 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.