This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 74.84 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 152,878 (5.4%) Reads that were too short: 28,680 (1.0%) Reads written (passing filters): 124,198 (4.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 32,021,314 bp (3.7%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 152878 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3753 696.4 0 3753 7 43080 696.4 0 43080 8 43820 696.4 0 43820 9 69 696.4 0 69 10 36 696.4 0 36 11 29 696.4 0 29 12 23 696.4 0 23 13 24 696.4 0 24 14 19 696.4 0 19 15 25 696.4 0 25 16 14 696.4 0 14 17 16 696.4 0 16 18 16 696.4 0 16 19 24 696.4 0 24 20 26 696.4 0 26 21 18 696.4 0 18 22 16 696.4 0 16 23 29 696.4 0 29 24 22 696.4 0 22 25 25 696.4 0 25 26 24 696.4 0 24 27 22 696.4 0 22 28 19 696.4 0 19 29 31 696.4 0 31 30 61 696.4 0 61 31 21 696.4 0 21 32 23 696.4 0 23 33 17 696.4 0 17 34 30 696.4 0 30 35 26 696.4 0 26 36 20 696.4 0 20 37 47 696.4 0 47 38 15 696.4 0 15 39 30 696.4 0 30 40 41 696.4 0 41 41 21 696.4 0 21 42 23 696.4 0 23 43 32 696.4 0 32 44 37 696.4 0 37 45 29 696.4 0 29 46 43 696.4 0 43 47 57 696.4 0 57 48 63 696.4 0 63 49 121 696.4 0 121 50 1502 696.4 0 1502 51 870 696.4 0 870 52 1134 696.4 0 1134 53 39 696.4 0 39 54 39 696.4 0 39 55 14 696.4 0 14 56 19 696.4 0 19 57 14 696.4 0 14 58 29 696.4 0 29 59 29 696.4 0 29 60 47 696.4 0 47 61 59 696.4 0 59 62 42 696.4 0 42 63 43 696.4 0 43 64 72 696.4 0 72 65 47 696.4 0 47 66 56 696.4 0 56 67 43 696.4 0 43 68 69 696.4 0 69 69 95 696.4 0 95 70 22 696.4 0 22 71 65 696.4 0 65 72 58 696.4 0 58 73 138 696.4 0 138 74 651 696.4 0 651 75 437 696.4 0 437 76 1568 696.4 0 1568 77 105 696.4 0 105 78 212 696.4 0 212 79 53 696.4 0 53 80 91 696.4 0 91 81 26 696.4 0 26 82 37 696.4 0 37 83 38 696.4 0 38 84 44 696.4 0 44 85 40 696.4 0 40 86 38 696.4 0 38 87 28 696.4 0 28 88 33 696.4 0 33 89 44 696.4 0 44 90 32 696.4 0 32 91 84 696.4 0 84 92 67 696.4 0 67 93 44 696.4 0 44 94 55 696.4 0 55 95 30 696.4 0 30 96 19 696.4 0 19 97 38 696.4 0 38 98 39 696.4 0 39 99 23 696.4 0 23 100 20 696.4 0 20 101 33 696.4 0 33 102 22 696.4 0 22 103 16 696.4 0 16 104 26 696.4 0 26 105 20 696.4 0 20 106 24 696.4 0 24 107 34 696.4 0 34 108 21 696.4 0 21 109 21 696.4 0 21 110 20 696.4 0 20 111 26 696.4 0 26 112 55 696.4 0 55 113 56 696.4 0 56 114 62 696.4 0 62 115 135 696.4 0 135 116 81 696.4 0 81 117 125 696.4 0 125 118 76 696.4 0 76 119 80 696.4 0 80 120 72 696.4 0 72 121 53 696.4 0 53 122 57 696.4 0 57 123 61 696.4 0 61 124 85 696.4 0 85 125 1073 696.4 0 1073 126 489 696.4 0 489 127 845 696.4 0 845 128 71 696.4 0 71 129 68 696.4 0 68 130 344 696.4 0 344 131 142 696.4 0 142 132 194 696.4 0 194 133 51 696.4 0 51 134 64 696.4 0 64 135 62 696.4 0 62 136 71 696.4 0 71 137 113 696.4 0 113 138 31 696.4 0 31 139 28 696.4 0 28 140 31 696.4 0 31 141 38 696.4 0 38 142 43 696.4 0 43 143 45 696.4 0 45 144 53 696.4 0 53 145 41 696.4 0 41 146 56 696.4 0 56 147 48 696.4 0 48 148 40 696.4 0 40 149 38 696.4 0 38 150 42 696.4 0 42 151 51 696.4 0 51 152 40 696.4 0 40 153 59 696.4 0 59 154 53 696.4 0 53 155 50 696.4 0 50 156 62 696.4 0 62 157 44 696.4 0 44 158 70 696.4 0 70 159 54 696.4 0 54 160 49 696.4 0 49 161 88 696.4 0 88 162 74 696.4 0 74 163 122 696.4 0 122 164 1535 696.4 0 1535 165 957 696.4 0 957 166 2321 696.4 0 2321 167 53 696.4 0 53 168 42 696.4 0 42 169 60 696.4 0 60 170 72 696.4 0 72 171 75 696.4 0 75 172 55 696.4 0 55 173 41 696.4 0 41 174 50 696.4 0 50 175 40 696.4 0 40 176 31 696.4 0 31 177 41 696.4 0 41 178 74 696.4 0 74 179 143 696.4 0 143 180 109 696.4 0 109 181 153 696.4 0 153 182 102 696.4 0 102 183 123 696.4 0 123 184 90 696.4 0 90 185 60 696.4 0 60 186 101 696.4 0 101 187 82 696.4 0 82 188 66 696.4 0 66 189 119 696.4 0 119 190 132 696.4 0 132 191 456 696.4 0 456 192 280 696.4 0 280 193 339 696.4 0 339 194 325 696.4 0 325 195 264 696.4 0 264 196 2509 696.4 0 2509 197 2159 696.4 0 2159 198 2735 696.4 0 2735 199 1266 696.4 0 1266 200 304 696.4 0 304 201 258 696.4 0 258 202 124 696.4 0 124 203 67 696.4 0 67 204 125 696.4 0 125 205 141 696.4 0 141 206 100 696.4 0 100 207 67 696.4 0 67 208 88 696.4 0 88 209 115 696.4 0 115 210 123 696.4 0 123 211 166 696.4 0 166 212 132 696.4 0 132 213 120 696.4 0 120 214 146 696.4 0 146 215 164 696.4 0 164 216 1049 696.4 0 1049 217 698 696.4 0 698 218 806 696.4 0 806 219 285 696.4 0 285 220 176 696.4 0 176 221 130 696.4 0 130 222 186 696.4 0 186 223 137 696.4 0 137 224 84 696.4 0 84 225 90 696.4 0 90 226 78 696.4 0 78 227 85 696.4 0 85 228 77 696.4 0 77 229 105 696.4 0 105 230 95 696.4 0 95 231 75 696.4 0 75 232 71 696.4 0 71 233 66 696.4 0 66 234 103 696.4 0 103 235 83 696.4 0 83 236 59 696.4 0 59 237 90 696.4 0 90 238 121 696.4 0 121 239 189 696.4 0 189 240 156 696.4 0 156 241 167 696.4 0 167 242 116 696.4 0 116 243 99 696.4 0 99 244 99 696.4 0 99 245 144 696.4 0 144 246 192 696.4 0 192 247 895 696.4 0 895 248 1375 696.4 0 1375 249 1541 696.4 0 1541 250 1249 696.4 0 1249 251 394 696.4 0 394 252 162 696.4 0 162 253 83 696.4 0 83 254 137 696.4 0 137 255 74 696.4 0 74 256 122 696.4 0 122 257 122 696.4 0 122 258 133 696.4 0 133 259 105 696.4 0 105 260 68 696.4 0 68 261 96 696.4 0 96 262 88 696.4 0 88 263 83 696.4 0 83 264 79 696.4 0 79 265 81 696.4 0 81 266 98 696.4 0 98 267 83 696.4 0 83 268 68 696.4 0 68 269 347 696.4 0 347 270 230 696.4 0 230 271 381 696.4 0 381 272 90 696.4 0 90 273 64 696.4 0 64 274 63 696.4 0 63 275 82 696.4 0 82 276 152 696.4 0 152 277 116 696.4 0 116 278 166 696.4 0 166 279 145 696.4 0 145 280 129 696.4 0 129 281 96 696.4 0 96 282 127 696.4 0 127 283 137 696.4 0 137 284 183 696.4 0 183 285 184 696.4 0 184 286 173 696.4 0 173 287 180 696.4 0 180 288 218 696.4 0 218 289 1487 696.4 0 1487 290 2035 696.4 0 2035 291 2884 696.4 0 2884 292 1598 696.4 0 1598 293 297 696.4 0 297 294 182 696.4 0 182 295 109 696.4 0 109 296 123 696.4 0 123 297 341 696.4 0 341 298 495 696.4 0 495 299 451 696.4 0 451 300 400 696.4 0 400 301 290 696.4 0 290 Finished in 5.59 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 124,198 Reads with adapters: 45,540 (36.7%) Reads that were too short: 287 (0.2%) Reads written (passing filters): 45,253 (36.4%) Total basepairs processed: 32,021,314 bp Total written (filtered): 12,477,816 bp (39.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 45540 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 21 0.0 3 0 0 13 8 16 389 0.0 3 0 334 30 25 17 44032 0.0 3 41104 2436 394 98 18 163 0.0 3 3 67 9 84 19 100 0.0 3 5 2 53 40 20 164 0.0 3 1 0 0 163 22 1 0.0 3 1 23 2 0.0 3 1 1 24 1 0.0 3 0 0 1 26 15 0.0 3 0 0 0 15 27 109 0.0 3 0 0 0 109 28 3 0.0 3 0 0 1 2 30 5 0.0 3 0 0 0 5 31 1 0.0 3 0 0 0 1 33 1 0.0 3 1 34 1 0.0 3 0 0 0 1 35 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 43 3 0.0 3 0 0 0 3 44 3 0.0 3 0 0 0 3 46 61 0.0 3 0 0 2 59 48 1 0.0 3 0 0 0 1 49 16 0.0 3 0 0 1 15 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 62 6 0.0 3 0 0 0 6 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 68 5 0.0 3 0 0 0 5 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 78 5 0.0 3 0 0 0 5 85 1 0.0 3 0 0 0 1 87 42 0.0 3 0 0 1 41 88 5 0.0 3 0 0 0 5 89 3 0.0 3 0 0 0 3 90 26 0.0 3 0 0 4 22 91 5 0.0 3 0 0 0 5 92 3 0.0 3 0 0 0 3 94 1 0.0 3 0 0 0 1 95 6 0.0 3 0 0 1 5 96 2 0.0 3 0 0 0 2 97 20 0.0 3 0 0 0 20 98 2 0.0 3 0 0 0 2 100 8 0.0 3 0 0 1 7 103 2 0.0 3 0 0 0 2 104 4 0.0 3 0 0 0 4 105 2 0.0 3 0 0 1 1 106 2 0.0 3 0 0 1 1 107 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 3 0.0 3 0 0 0 3 114 3 0.0 3 0 0 0 3 115 3 0.0 3 0 0 0 3 116 6 0.0 3 0 0 0 6 117 43 0.0 3 0 0 0 43 118 16 0.0 3 0 0 0 16 119 1 0.0 3 0 0 0 1 120 13 0.0 3 0 0 0 13 121 5 0.0 3 0 0 0 5 122 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 1 127 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 139 3 0.0 3 0 0 0 3 140 2 0.0 3 0 0 0 2 141 8 0.0 3 0 0 0 8 143 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 166 3 0.0 3 0 0 0 3 167 4 0.0 3 0 0 1 3 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 178 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 1 187 2 0.0 3 0 0 1 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 205 8 0.0 3 0 0 0 8 206 1 0.0 3 0 0 0 1 209 3 0.0 3 0 0 0 3 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 27 0.0 3 0 0 2 25 218 7 0.0 3 0 0 0 7 219 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 222 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 1 227 2 0.0 3 0 0 0 2 236 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 2 0.0 3 0 0 0 2 270 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 8 0.0 3 0 0 0 8 280 1 0.0 3 0 0 0 1 281 13 0.0 3 0 0 0 13 282 3 0.0 3 0 0 1 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.57 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 45,253 Reads with adapters: 241 (0.5%) Reads written (passing filters): 45,253 (100.0%) Total basepairs processed: 12,477,816 bp Total written (filtered): 12,477,048 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 241 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.1% C: 43.6% G: 27.4% T: 10.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 218 707.1 0 218 4 7 176.8 0 7 5 11 44.2 0 11 6 4 11.0 0 4 7 1 2.8 0 1