This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.84 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 131,921 (4.6%) Reads that were too short: 14,934 (0.5%) Reads written (passing filters): 116,987 (4.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 31,356,400 bp (3.7%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 131921 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3975 696.4 0 3975 7 43642 696.4 0 43642 8 45454 696.4 0 45454 9 56 696.4 0 56 10 39 696.4 0 39 11 48 696.4 0 48 12 30 696.4 0 30 13 34 696.4 0 34 14 30 696.4 0 30 15 25 696.4 0 25 16 19 696.4 0 19 17 22 696.4 0 22 18 18 696.4 0 18 19 23 696.4 0 23 20 22 696.4 0 22 21 23 696.4 0 23 22 23 696.4 0 23 23 31 696.4 0 31 24 23 696.4 0 23 25 26 696.4 0 26 26 26 696.4 0 26 27 30 696.4 0 30 28 42 696.4 0 42 29 25 696.4 0 25 30 82 696.4 0 82 31 19 696.4 0 19 32 27 696.4 0 27 33 46 696.4 0 46 34 25 696.4 0 25 35 27 696.4 0 27 36 32 696.4 0 32 37 43 696.4 0 43 38 24 696.4 0 24 39 42 696.4 0 42 40 58 696.4 0 58 41 30 696.4 0 30 42 31 696.4 0 31 43 54 696.4 0 54 44 32 696.4 0 32 45 34 696.4 0 34 46 27 696.4 0 27 47 56 696.4 0 56 48 54 696.4 0 54 49 82 696.4 0 82 50 1363 696.4 0 1363 51 709 696.4 0 709 52 946 696.4 0 946 53 31 696.4 0 31 54 26 696.4 0 26 55 18 696.4 0 18 56 12 696.4 0 12 57 27 696.4 0 27 58 23 696.4 0 23 59 22 696.4 0 22 60 34 696.4 0 34 61 36 696.4 0 36 62 20 696.4 0 20 63 58 696.4 0 58 64 45 696.4 0 45 65 48 696.4 0 48 66 39 696.4 0 39 67 40 696.4 0 40 68 56 696.4 0 56 69 96 696.4 0 96 70 28 696.4 0 28 71 32 696.4 0 32 72 52 696.4 0 52 73 59 696.4 0 59 74 332 696.4 0 332 75 252 696.4 0 252 76 771 696.4 0 771 77 41 696.4 0 41 78 80 696.4 0 80 79 41 696.4 0 41 80 52 696.4 0 52 81 29 696.4 0 29 82 24 696.4 0 24 83 26 696.4 0 26 84 31 696.4 0 31 85 31 696.4 0 31 86 30 696.4 0 30 87 32 696.4 0 32 88 27 696.4 0 27 89 21 696.4 0 21 90 31 696.4 0 31 91 38 696.4 0 38 92 22 696.4 0 22 93 23 696.4 0 23 94 19 696.4 0 19 95 22 696.4 0 22 96 22 696.4 0 22 97 29 696.4 0 29 98 40 696.4 0 40 99 38 696.4 0 38 100 39 696.4 0 39 101 37 696.4 0 37 102 32 696.4 0 32 103 49 696.4 0 49 104 30 696.4 0 30 105 27 696.4 0 27 106 38 696.4 0 38 107 40 696.4 0 40 108 31 696.4 0 31 109 27 696.4 0 27 110 30 696.4 0 30 111 43 696.4 0 43 112 46 696.4 0 46 113 39 696.4 0 39 114 59 696.4 0 59 115 91 696.4 0 91 116 76 696.4 0 76 117 79 696.4 0 79 118 136 696.4 0 136 119 64 696.4 0 64 120 48 696.4 0 48 121 46 696.4 0 46 122 60 696.4 0 60 123 77 696.4 0 77 124 75 696.4 0 75 125 865 696.4 0 865 126 343 696.4 0 343 127 561 696.4 0 561 128 60 696.4 0 60 129 72 696.4 0 72 130 187 696.4 0 187 131 102 696.4 0 102 132 126 696.4 0 126 133 49 696.4 0 49 134 41 696.4 0 41 135 71 696.4 0 71 136 43 696.4 0 43 137 113 696.4 0 113 138 48 696.4 0 48 139 40 696.4 0 40 140 21 696.4 0 21 141 37 696.4 0 37 142 47 696.4 0 47 143 26 696.4 0 26 144 36 696.4 0 36 145 45 696.4 0 45 146 42 696.4 0 42 147 35 696.4 0 35 148 35 696.4 0 35 149 38 696.4 0 38 150 45 696.4 0 45 151 43 696.4 0 43 152 33 696.4 0 33 153 52 696.4 0 52 154 50 696.4 0 50 155 33 696.4 0 33 156 36 696.4 0 36 157 58 696.4 0 58 158 65 696.4 0 65 159 53 696.4 0 53 160 47 696.4 0 47 161 80 696.4 0 80 162 76 696.4 0 76 163 109 696.4 0 109 164 1467 696.4 0 1467 165 888 696.4 0 888 166 2213 696.4 0 2213 167 40 696.4 0 40 168 53 696.4 0 53 169 33 696.4 0 33 170 30 696.4 0 30 171 47 696.4 0 47 172 30 696.4 0 30 173 28 696.4 0 28 174 38 696.4 0 38 175 32 696.4 0 32 176 43 696.4 0 43 177 53 696.4 0 53 178 57 696.4 0 57 179 53 696.4 0 53 180 36 696.4 0 36 181 82 696.4 0 82 182 57 696.4 0 57 183 88 696.4 0 88 184 52 696.4 0 52 185 46 696.4 0 46 186 74 696.4 0 74 187 65 696.4 0 65 188 41 696.4 0 41 189 75 696.4 0 75 190 77 696.4 0 77 191 264 696.4 0 264 192 130 696.4 0 130 193 206 696.4 0 206 194 123 696.4 0 123 195 144 696.4 0 144 196 1334 696.4 0 1334 197 1111 696.4 0 1111 198 1346 696.4 0 1346 199 627 696.4 0 627 200 146 696.4 0 146 201 141 696.4 0 141 202 79 696.4 0 79 203 58 696.4 0 58 204 85 696.4 0 85 205 132 696.4 0 132 206 92 696.4 0 92 207 58 696.4 0 58 208 73 696.4 0 73 209 86 696.4 0 86 210 89 696.4 0 89 211 90 696.4 0 90 212 84 696.4 0 84 213 110 696.4 0 110 214 85 696.4 0 85 215 88 696.4 0 88 216 528 696.4 0 528 217 385 696.4 0 385 218 386 696.4 0 386 219 159 696.4 0 159 220 54 696.4 0 54 221 59 696.4 0 59 222 85 696.4 0 85 223 78 696.4 0 78 224 77 696.4 0 77 225 77 696.4 0 77 226 40 696.4 0 40 227 60 696.4 0 60 228 52 696.4 0 52 229 56 696.4 0 56 230 58 696.4 0 58 231 51 696.4 0 51 232 42 696.4 0 42 233 36 696.4 0 36 234 57 696.4 0 57 235 60 696.4 0 60 236 50 696.4 0 50 237 59 696.4 0 59 238 64 696.4 0 64 239 125 696.4 0 125 240 114 696.4 0 114 241 89 696.4 0 89 242 70 696.4 0 70 243 50 696.4 0 50 244 52 696.4 0 52 245 40 696.4 0 40 246 84 696.4 0 84 247 369 696.4 0 369 248 761 696.4 0 761 249 867 696.4 0 867 250 880 696.4 0 880 251 173 696.4 0 173 252 75 696.4 0 75 253 51 696.4 0 51 254 57 696.4 0 57 255 52 696.4 0 52 256 60 696.4 0 60 257 56 696.4 0 56 258 68 696.4 0 68 259 64 696.4 0 64 260 55 696.4 0 55 261 43 696.4 0 43 262 58 696.4 0 58 263 50 696.4 0 50 264 57 696.4 0 57 265 34 696.4 0 34 266 56 696.4 0 56 267 66 696.4 0 66 268 57 696.4 0 57 269 406 696.4 0 406 270 187 696.4 0 187 271 373 696.4 0 373 272 55 696.4 0 55 273 40 696.4 0 40 274 41 696.4 0 41 275 54 696.4 0 54 276 70 696.4 0 70 277 61 696.4 0 61 278 71 696.4 0 71 279 101 696.4 0 101 280 61 696.4 0 61 281 63 696.4 0 63 282 65 696.4 0 65 283 76 696.4 0 76 284 79 696.4 0 79 285 74 696.4 0 74 286 78 696.4 0 78 287 71 696.4 0 71 288 77 696.4 0 77 289 511 696.4 0 511 290 770 696.4 0 770 291 1268 696.4 0 1268 292 726 696.4 0 726 293 142 696.4 0 142 294 103 696.4 0 103 295 50 696.4 0 50 296 81 696.4 0 81 297 182 696.4 0 182 298 155 696.4 0 155 299 182 696.4 0 182 300 132 696.4 0 132 301 114 696.4 0 114 Finished in 5.81 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 116,987 Reads with adapters: 47,106 (40.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 47,072 (40.2%) Total basepairs processed: 31,356,400 bp Total written (filtered): 12,838,462 bp (40.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 47106 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 0 4 18 25 0.0 3 0 0 25 19 476 0.0 3 0 450 10 4 12 20 46270 0.0 4 45518 708 23 2 19 21 135 0.0 4 26 107 2 22 13 0.0 4 5 1 5 2 23 35 0.0 4 1 0 0 29 5 24 2 0.0 4 1 0 0 0 1 30 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 35 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 1 62 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 84 7 0.0 4 0 0 0 0 7 89 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 1 126 3 0.0 4 0 0 0 0 3 127 95 0.0 4 0 0 0 0 95 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 185 2 0.0 4 0 0 0 0 2 186 5 0.0 4 0 0 0 0 5 188 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 1 221 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 244 2 0.0 4 0 0 0 0 2 262 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.63 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 47,072 Reads with adapters: 3,376 (7.2%) Reads written (passing filters): 47,072 (100.0%) Total basepairs processed: 12,838,462 bp Total written (filtered): 12,800,763 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3376 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 3.7% G: 92.6% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 41 735.5 0 41 4 21 183.9 0 21 5 1 46.0 0 1 6 1 11.5 0 1 9 74 0.2 0 1 73 10 702 0.0 1 14 688 11 2518 0.0 1 35 2483 12 6 0.0 1 1 5 14 1 0.0 1 0 1 15 1 0.0 1 0 1 115 1 0.0 1 1 209 9 0.0 1 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.