This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.90 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 293,172 (10.3%) Reads that were too short: 104,072 (3.6%) Reads written (passing filters): 189,100 (6.6%) Total basepairs processed: 858,556,748 bp Total written (filtered): 50,513,696 bp (5.9%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 293172 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1933 696.4 0 1933 7 75248 696.4 0 75248 8 1012 696.4 0 1012 9 71090 696.4 0 71090 10 80 696.4 0 80 11 26 696.4 0 26 12 18 696.4 0 18 13 27 696.4 0 27 14 31 696.4 0 31 15 42 696.4 0 42 16 44 696.4 0 44 17 37 696.4 0 37 18 33 696.4 0 33 19 33 696.4 0 33 20 25 696.4 0 25 21 20 696.4 0 20 22 31 696.4 0 31 23 20 696.4 0 20 24 34 696.4 0 34 25 24 696.4 0 24 26 32 696.4 0 32 27 523 696.4 0 523 28 67 696.4 0 67 29 25 696.4 0 25 30 31 696.4 0 31 31 26 696.4 0 26 32 28 696.4 0 28 33 32 696.4 0 32 34 28 696.4 0 28 35 38 696.4 0 38 36 30 696.4 0 30 37 28 696.4 0 28 38 24 696.4 0 24 39 35 696.4 0 35 40 38 696.4 0 38 41 34 696.4 0 34 42 55 696.4 0 55 43 34 696.4 0 34 44 42 696.4 0 42 45 27 696.4 0 27 46 30 696.4 0 30 47 29 696.4 0 29 48 32 696.4 0 32 49 33 696.4 0 33 50 60 696.4 0 60 51 34 696.4 0 34 52 36 696.4 0 36 53 31 696.4 0 31 54 65 696.4 0 65 55 76 696.4 0 76 56 99 696.4 0 99 57 1255 696.4 0 1255 58 1303 696.4 0 1303 59 757 696.4 0 757 60 31 696.4 0 31 61 28 696.4 0 28 62 28 696.4 0 28 63 33 696.4 0 33 64 41 696.4 0 41 65 30 696.4 0 30 66 23 696.4 0 23 67 32 696.4 0 32 68 27 696.4 0 27 69 21 696.4 0 21 70 20 696.4 0 20 71 21 696.4 0 21 72 35 696.4 0 35 73 21 696.4 0 21 74 25 696.4 0 25 75 20 696.4 0 20 76 21 696.4 0 21 77 34 696.4 0 34 78 43 696.4 0 43 79 70 696.4 0 70 80 85 696.4 0 85 81 62 696.4 0 62 82 43 696.4 0 43 83 63 696.4 0 63 84 87 696.4 0 87 85 50 696.4 0 50 86 80 696.4 0 80 87 74 696.4 0 74 88 220 696.4 0 220 89 127 696.4 0 127 90 206 696.4 0 206 91 115 696.4 0 115 92 137 696.4 0 137 93 67 696.4 0 67 94 60 696.4 0 60 95 58 696.4 0 58 96 63 696.4 0 63 97 43 696.4 0 43 98 63 696.4 0 63 99 71 696.4 0 71 100 58 696.4 0 58 101 65 696.4 0 65 102 68 696.4 0 68 103 68 696.4 0 68 104 94 696.4 0 94 105 147 696.4 0 147 106 124 696.4 0 124 107 101 696.4 0 101 108 99 696.4 0 99 109 84 696.4 0 84 110 64 696.4 0 64 111 42 696.4 0 42 112 56 696.4 0 56 113 56 696.4 0 56 114 77 696.4 0 77 115 96 696.4 0 96 116 62 696.4 0 62 117 79 696.4 0 79 118 50 696.4 0 50 119 63 696.4 0 63 120 79 696.4 0 79 121 154 696.4 0 154 122 787 696.4 0 787 123 546 696.4 0 546 124 456 696.4 0 456 125 86 696.4 0 86 126 62 696.4 0 62 127 133 696.4 0 133 128 3381 696.4 0 3381 129 78 696.4 0 78 130 117 696.4 0 117 131 110 696.4 0 110 132 73 696.4 0 73 133 58 696.4 0 58 134 90 696.4 0 90 135 122 696.4 0 122 136 71 696.4 0 71 137 74 696.4 0 74 138 75 696.4 0 75 139 72 696.4 0 72 140 85 696.4 0 85 141 322 696.4 0 322 142 363 696.4 0 363 143 257 696.4 0 257 144 94 696.4 0 94 145 92 696.4 0 92 146 133 696.4 0 133 147 206 696.4 0 206 148 231 696.4 0 231 149 324 696.4 0 324 150 315 696.4 0 315 151 4588 696.4 0 4588 152 6104 696.4 0 6104 153 4303 696.4 0 4303 154 124 696.4 0 124 155 106 696.4 0 106 156 93 696.4 0 93 157 420 696.4 0 420 158 249 696.4 0 249 159 249 696.4 0 249 160 81 696.4 0 81 161 141 696.4 0 141 162 258 696.4 0 258 163 162 696.4 0 162 164 92 696.4 0 92 165 68 696.4 0 68 166 77 696.4 0 77 167 100 696.4 0 100 168 95 696.4 0 95 169 92 696.4 0 92 170 152 696.4 0 152 171 102 696.4 0 102 172 105 696.4 0 105 173 40 696.4 0 40 174 75 696.4 0 75 175 102 696.4 0 102 176 166 696.4 0 166 177 98 696.4 0 98 178 78 696.4 0 78 179 86 696.4 0 86 180 68 696.4 0 68 181 85 696.4 0 85 182 254 696.4 0 254 183 200 696.4 0 200 184 163 696.4 0 163 185 90 696.4 0 90 186 197 696.4 0 197 187 190 696.4 0 190 188 155 696.4 0 155 189 120 696.4 0 120 190 70 696.4 0 70 191 69 696.4 0 69 192 51 696.4 0 51 193 74 696.4 0 74 194 119 696.4 0 119 195 171 696.4 0 171 196 234 696.4 0 234 197 132 696.4 0 132 198 105 696.4 0 105 199 73 696.4 0 73 200 100 696.4 0 100 201 172 696.4 0 172 202 140 696.4 0 140 203 110 696.4 0 110 204 113 696.4 0 113 205 156 696.4 0 156 206 167 696.4 0 167 207 209 696.4 0 209 208 387 696.4 0 387 209 869 696.4 0 869 210 1083 696.4 0 1083 211 1296 696.4 0 1296 212 1794 696.4 0 1794 213 23954 696.4 0 23954 214 38946 696.4 0 38946 215 18640 696.4 0 18640 216 450 696.4 0 450 217 133 696.4 0 133 218 114 696.4 0 114 219 116 696.4 0 116 220 263 696.4 0 263 221 1355 696.4 0 1355 222 1560 696.4 0 1560 223 1392 696.4 0 1392 224 156 696.4 0 156 225 87 696.4 0 87 226 90 696.4 0 90 227 84 696.4 0 84 228 78 696.4 0 78 229 68 696.4 0 68 230 57 696.4 0 57 231 111 696.4 0 111 232 113 696.4 0 113 233 125 696.4 0 125 234 111 696.4 0 111 235 107 696.4 0 107 236 86 696.4 0 86 237 73 696.4 0 73 238 75 696.4 0 75 239 70 696.4 0 70 240 86 696.4 0 86 241 85 696.4 0 85 242 101 696.4 0 101 243 125 696.4 0 125 244 178 696.4 0 178 245 149 696.4 0 149 246 105 696.4 0 105 247 73 696.4 0 73 248 64 696.4 0 64 249 86 696.4 0 86 250 88 696.4 0 88 251 79 696.4 0 79 252 76 696.4 0 76 253 103 696.4 0 103 254 91 696.4 0 91 255 125 696.4 0 125 256 133 696.4 0 133 257 344 696.4 0 344 258 404 696.4 0 404 259 360 696.4 0 360 260 186 696.4 0 186 261 194 696.4 0 194 262 175 696.4 0 175 263 528 696.4 0 528 264 254 696.4 0 254 265 208 696.4 0 208 266 114 696.4 0 114 267 63 696.4 0 63 268 86 696.4 0 86 269 176 696.4 0 176 270 249 696.4 0 249 271 161 696.4 0 161 272 148 696.4 0 148 273 184 696.4 0 184 274 124 696.4 0 124 275 165 696.4 0 165 276 175 696.4 0 175 277 159 696.4 0 159 278 129 696.4 0 129 279 104 696.4 0 104 280 90 696.4 0 90 281 204 696.4 0 204 282 177 696.4 0 177 283 229 696.4 0 229 284 179 696.4 0 179 285 159 696.4 0 159 286 149 696.4 0 149 287 83 696.4 0 83 288 86 696.4 0 86 289 104 696.4 0 104 290 156 696.4 0 156 291 146 696.4 0 146 292 96 696.4 0 96 293 85 696.4 0 85 294 111 696.4 0 111 295 135 696.4 0 135 296 157 696.4 0 157 297 140 696.4 0 140 298 129 696.4 0 129 299 110 696.4 0 110 300 97 696.4 0 97 301 75 696.4 0 75 Finished in 8.92 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 189,100 Reads with adapters: 76,444 (40.4%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 76,420 (40.4%) Total basepairs processed: 50,513,696 bp Total written (filtered): 20,896,524 bp (41.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 76444 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 20 0.0 3 0 0 19 1 19 584 0.0 3 0 563 18 2 1 20 75196 0.0 4 73890 1253 43 8 2 21 175 0.0 4 26 147 2 22 14 0.0 4 4 0 10 23 48 0.0 4 1 0 0 43 4 24 1 0.0 4 0 0 0 0 1 27 40 0.0 4 0 0 0 0 40 28 2 0.0 4 1 0 0 0 1 29 1 0.0 4 1 42 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 85 2 0.0 4 2 104 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 322 0.0 4 0 0 0 0 322 128 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 137 1 0.0 4 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 182 1 0.0 4 1 187 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 1 1 235 2 0.0 4 0 0 0 0 2 237 1 0.0 4 0 0 0 0 1 262 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.62 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 76,420 Reads with adapters: 1,731 (2.3%) Reads written (passing filters): 76,420 (100.0%) Total basepairs processed: 20,896,524 bp Total written (filtered): 20,735,208 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1731 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 38.5% C: 4.6% G: 54.8% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 110 1194.1 0 110 4 14 298.5 0 14 5 1 74.6 0 1 6 1 18.7 0 1 8 1 1.2 0 1 9 5 0.3 0 0 5 10 25 0.1 1 2 23 11 929 0.0 1 14 915 12 56 0.0 1 2 54 13 3 0.0 1 0 3 14 1 0.0 1 0 1 15 2 0.0 1 0 2 16 1 0.0 1 0 1 20 4 0.0 1 3 1 123 1 0.0 1 1 152 2 0.0 1 0 2 153 1 0.0 1 0 1 191 1 0.0 1 0 1 193 1 0.0 1 0 1 210 8 0.0 1 4 4 211 2 0.0 1 0 2 225 5 0.0 1 5 226 1 0.0 1 1 260 2 0.0 1 2 261 539 0.0 1 504 35 262 15 0.0 1 15