This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.88 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 342,450 (12.0%) Reads that were too short: 17,603 (0.6%) Reads written (passing filters): 324,847 (11.4%) Total basepairs processed: 858,556,748 bp Total written (filtered): 71,888,748 bp (8.4%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 342450 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 803 696.4 0 803 7 1750 696.4 0 1750 8 25654 696.4 0 25654 9 20527 696.4 0 20527 10 72 696.4 0 72 11 62 696.4 0 62 12 64 696.4 0 64 13 48 696.4 0 48 14 62 696.4 0 62 15 60 696.4 0 60 16 48 696.4 0 48 17 51 696.4 0 51 18 70 696.4 0 70 19 82 696.4 0 82 20 94 696.4 0 94 21 85 696.4 0 85 22 116 696.4 0 116 23 186 696.4 0 186 24 174 696.4 0 174 25 67 696.4 0 67 26 73 696.4 0 73 27 84 696.4 0 84 28 69 696.4 0 69 29 75 696.4 0 75 30 103 696.4 0 103 31 87 696.4 0 87 32 90 696.4 0 90 33 88 696.4 0 88 34 97 696.4 0 97 35 102 696.4 0 102 36 141 696.4 0 141 37 128 696.4 0 128 38 171 696.4 0 171 39 109 696.4 0 109 40 130 696.4 0 130 41 91 696.4 0 91 42 151 696.4 0 151 43 95 696.4 0 95 44 119 696.4 0 119 45 199 696.4 0 199 46 225 696.4 0 225 47 363 696.4 0 363 48 703 696.4 0 703 49 1461 696.4 0 1461 50 415 696.4 0 415 51 532 696.4 0 532 52 988 696.4 0 988 53 541 696.4 0 541 54 498 696.4 0 498 55 748 696.4 0 748 56 893 696.4 0 893 57 996 696.4 0 996 58 714 696.4 0 714 59 1513 696.4 0 1513 60 2034 696.4 0 2034 61 3824 696.4 0 3824 62 57772 696.4 0 57772 63 51625 696.4 0 51625 64 51230 696.4 0 51230 65 1779 696.4 0 1779 66 80 696.4 0 80 67 57 696.4 0 57 68 67 696.4 0 67 69 80 696.4 0 80 70 95 696.4 0 95 71 390 696.4 0 390 72 513 696.4 0 513 73 328 696.4 0 328 74 140 696.4 0 140 75 186 696.4 0 186 76 1428 696.4 0 1428 77 1267 696.4 0 1267 78 853 696.4 0 853 79 65 696.4 0 65 80 94 696.4 0 94 81 66 696.4 0 66 82 71 696.4 0 71 83 113 696.4 0 113 84 50 696.4 0 50 85 88 696.4 0 88 86 71 696.4 0 71 87 81 696.4 0 81 88 54 696.4 0 54 89 65 696.4 0 65 90 65 696.4 0 65 91 88 696.4 0 88 92 72 696.4 0 72 93 126 696.4 0 126 94 83 696.4 0 83 95 65 696.4 0 65 96 50 696.4 0 50 97 45 696.4 0 45 98 83 696.4 0 83 99 71 696.4 0 71 100 63 696.4 0 63 101 55 696.4 0 55 102 65 696.4 0 65 103 84 696.4 0 84 104 93 696.4 0 93 105 139 696.4 0 139 106 101 696.4 0 101 107 440 696.4 0 440 108 464 696.4 0 464 109 499 696.4 0 499 110 233 696.4 0 233 111 139 696.4 0 139 112 181 696.4 0 181 113 58 696.4 0 58 114 68 696.4 0 68 115 55 696.4 0 55 116 67 696.4 0 67 117 98 696.4 0 98 118 70 696.4 0 70 119 65 696.4 0 65 120 69 696.4 0 69 121 77 696.4 0 77 122 53 696.4 0 53 123 52 696.4 0 52 124 72 696.4 0 72 125 91 696.4 0 91 126 156 696.4 0 156 127 270 696.4 0 270 128 169 696.4 0 169 129 98 696.4 0 98 130 86 696.4 0 86 131 107 696.4 0 107 132 89 696.4 0 89 133 95 696.4 0 95 134 107 696.4 0 107 135 102 696.4 0 102 136 73 696.4 0 73 137 143 696.4 0 143 138 134 696.4 0 134 139 201 696.4 0 201 140 201 696.4 0 201 141 288 696.4 0 288 142 532 696.4 0 532 143 1101 696.4 0 1101 144 11642 696.4 0 11642 145 10234 696.4 0 10234 146 5485 696.4 0 5485 147 192 696.4 0 192 148 91 696.4 0 91 149 168 696.4 0 168 150 97 696.4 0 97 151 156 696.4 0 156 152 156 696.4 0 156 153 124 696.4 0 124 154 185 696.4 0 185 155 223 696.4 0 223 156 2491 696.4 0 2491 157 1695 696.4 0 1695 158 1135 696.4 0 1135 159 219 696.4 0 219 160 218 696.4 0 218 161 290 696.4 0 290 162 463 696.4 0 463 163 713 696.4 0 713 164 6404 696.4 0 6404 165 6063 696.4 0 6063 166 9916 696.4 0 9916 167 264 696.4 0 264 168 193 696.4 0 193 169 1559 696.4 0 1559 170 2216 696.4 0 2216 171 2853 696.4 0 2853 172 2884 696.4 0 2884 173 973 696.4 0 973 174 642 696.4 0 642 175 130 696.4 0 130 176 70 696.4 0 70 177 127 696.4 0 127 178 116 696.4 0 116 179 251 696.4 0 251 180 162 696.4 0 162 181 190 696.4 0 190 182 125 696.4 0 125 183 122 696.4 0 122 184 249 696.4 0 249 185 244 696.4 0 244 186 313 696.4 0 313 187 134 696.4 0 134 188 225 696.4 0 225 189 1437 696.4 0 1437 190 2023 696.4 0 2023 191 1829 696.4 0 1829 192 604 696.4 0 604 193 72 696.4 0 72 194 70 696.4 0 70 195 55 696.4 0 55 196 115 696.4 0 115 197 157 696.4 0 157 198 238 696.4 0 238 199 119 696.4 0 119 200 80 696.4 0 80 201 62 696.4 0 62 202 73 696.4 0 73 203 103 696.4 0 103 204 95 696.4 0 95 205 88 696.4 0 88 206 127 696.4 0 127 207 94 696.4 0 94 208 131 696.4 0 131 209 90 696.4 0 90 210 63 696.4 0 63 211 69 696.4 0 69 212 112 696.4 0 112 213 123 696.4 0 123 214 120 696.4 0 120 215 212 696.4 0 212 216 292 696.4 0 292 217 317 696.4 0 317 218 275 696.4 0 275 219 88 696.4 0 88 220 77 696.4 0 77 221 83 696.4 0 83 222 58 696.4 0 58 223 92 696.4 0 92 224 116 696.4 0 116 225 228 696.4 0 228 226 223 696.4 0 223 227 193 696.4 0 193 228 187 696.4 0 187 229 513 696.4 0 513 230 379 696.4 0 379 231 181 696.4 0 181 232 85 696.4 0 85 233 81 696.4 0 81 234 73 696.4 0 73 235 81 696.4 0 81 236 124 696.4 0 124 237 133 696.4 0 133 238 224 696.4 0 224 239 126 696.4 0 126 240 95 696.4 0 95 241 65 696.4 0 65 242 70 696.4 0 70 243 108 696.4 0 108 244 106 696.4 0 106 245 196 696.4 0 196 246 176 696.4 0 176 247 256 696.4 0 256 248 174 696.4 0 174 249 97 696.4 0 97 250 78 696.4 0 78 251 101 696.4 0 101 252 98 696.4 0 98 253 76 696.4 0 76 254 96 696.4 0 96 255 68 696.4 0 68 256 80 696.4 0 80 257 79 696.4 0 79 258 90 696.4 0 90 259 170 696.4 0 170 260 378 696.4 0 378 261 474 696.4 0 474 262 407 696.4 0 407 263 237 696.4 0 237 264 206 696.4 0 206 265 170 696.4 0 170 266 244 696.4 0 244 267 180 696.4 0 180 268 147 696.4 0 147 269 66 696.4 0 66 270 92 696.4 0 92 271 138 696.4 0 138 272 127 696.4 0 127 273 129 696.4 0 129 274 115 696.4 0 115 275 98 696.4 0 98 276 94 696.4 0 94 277 225 696.4 0 225 278 212 696.4 0 212 279 170 696.4 0 170 280 184 696.4 0 184 281 130 696.4 0 130 282 98 696.4 0 98 283 79 696.4 0 79 284 205 696.4 0 205 285 275 696.4 0 275 286 381 696.4 0 381 287 1034 696.4 0 1034 288 902 696.4 0 902 289 620 696.4 0 620 290 226 696.4 0 226 291 125 696.4 0 125 292 117 696.4 0 117 293 184 696.4 0 184 294 113 696.4 0 113 295 156 696.4 0 156 296 178 696.4 0 178 297 154 696.4 0 154 298 180 696.4 0 180 299 148 696.4 0 148 300 82 696.4 0 82 301 115 696.4 0 115 Finished in 12.41 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 324,847 Reads with adapters: 27,439 (8.4%) Reads that were too short: 625 (0.2%) Reads written (passing filters): 26,814 (8.3%) Total basepairs processed: 71,888,748 bp Total written (filtered): 7,215,004 bp (10.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 27439 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 20 0.0 3 0 0 13 7 16 244 0.0 3 0 214 21 9 17 25049 0.0 3 23461 1296 236 56 18 73 0.0 3 2 55 8 8 19 127 0.0 3 34 13 46 34 20 136 0.0 3 0 0 0 136 21 9 0.0 3 0 0 0 9 23 1 0.0 3 1 24 4 0.0 3 0 0 0 4 25 1 0.0 3 0 0 0 1 26 6 0.0 3 1 0 0 5 27 3 0.0 3 0 0 0 3 28 20 0.0 3 0 0 0 20 29 10 0.0 3 1 1 0 8 32 4 0.0 3 0 0 0 4 34 1 0.0 3 1 35 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 16 0.0 3 0 0 0 16 40 4 0.0 3 0 0 0 4 43 2 0.0 3 0 0 0 2 46 3 0.0 3 0 0 0 3 48 6 0.0 3 0 1 0 5 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 9 0.0 3 0 0 0 9 54 3 0.0 3 0 0 0 3 55 3 0.0 3 0 0 0 3 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 1 1 59 3 0.0 3 0 0 0 3 60 10 0.0 3 0 0 0 10 61 9 0.0 3 0 0 0 9 62 8 0.0 3 0 0 0 8 63 8 0.0 3 0 0 1 7 68 2 0.0 3 0 0 0 2 69 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 3 0.0 3 0 0 0 3 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 7 0.0 3 0 0 0 7 96 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 102 3 0.0 3 0 0 0 3 103 3 0.0 3 0 0 0 3 104 22 0.0 3 0 0 1 21 105 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 0 3 107 11 0.0 3 0 0 0 11 108 1 0.0 3 0 0 0 1 109 38 0.0 3 0 0 2 36 110 5 0.0 3 0 0 0 5 112 3 0.0 3 0 0 0 3 113 4 0.0 3 0 0 1 3 114 34 0.0 3 0 0 0 34 115 3 0.0 3 0 0 0 3 116 9 0.0 3 0 0 2 7 117 22 0.0 3 0 0 0 22 118 2 0.0 3 0 0 0 2 119 5 0.0 3 0 0 0 5 120 5 0.0 3 0 0 1 4 121 1 0.0 3 0 0 0 1 122 11 0.0 3 0 0 0 11 123 5 0.0 3 0 0 1 4 124 20 0.0 3 0 0 0 20 125 5 0.0 3 0 0 0 5 126 6 0.0 3 0 0 0 6 127 8 0.0 3 0 0 0 8 128 24 0.0 3 0 0 2 22 129 695 0.0 3 0 0 8 687 130 96 0.0 3 0 0 1 95 131 8 0.0 3 0 0 1 7 132 83 0.0 3 0 0 0 83 133 23 0.0 3 0 0 1 22 134 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 7 0.0 3 0 0 0 7 143 2 0.0 3 0 0 0 2 144 8 0.0 3 0 0 0 8 145 6 0.0 3 0 0 1 5 146 2 0.0 3 0 0 0 2 147 3 0.0 3 0 0 0 3 148 6 0.0 3 0 0 0 6 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 1 1 151 3 0.0 3 0 0 1 2 152 1 0.0 3 0 0 0 1 153 4 0.0 3 0 0 0 4 154 4 0.0 3 0 0 0 4 155 19 0.0 3 0 0 0 19 156 9 0.0 3 0 0 0 9 157 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 159 1 0.0 3 0 0 0 1 164 4 0.0 3 0 0 0 4 166 4 0.0 3 0 0 0 4 167 4 0.0 3 0 0 0 4 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 175 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 22 0.0 3 0 0 0 22 181 13 0.0 3 0 0 0 13 182 7 0.0 3 0 0 0 7 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 189 8 0.0 3 0 0 0 8 190 6 0.0 3 0 0 0 6 191 3 0.0 3 0 0 0 3 192 6 0.0 3 0 0 0 6 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 197 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 202 2 0.0 3 0 0 0 2 204 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 207 2 0.0 3 0 0 0 2 208 4 0.0 3 0 0 0 4 210 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 215 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 229 6 0.0 3 0 0 0 6 230 65 0.0 3 0 0 4 61 231 106 0.0 3 0 0 4 102 232 7 0.0 3 0 0 0 7 233 2 0.0 3 0 0 0 2 234 1 0.0 3 0 0 0 1 236 4 0.0 3 0 0 0 4 239 5 0.0 3 0 0 0 5 240 1 0.0 3 0 0 0 1 241 2 0.0 3 0 0 0 2 242 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 5 0.0 3 0 0 1 4 281 2 0.0 3 0 0 0 2 282 3 0.0 3 0 0 0 3 288 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 26,814 Reads with adapters: 192 (0.7%) Reads written (passing filters): 26,814 (100.0%) Total basepairs processed: 7,215,004 bp Total written (filtered): 7,214,342 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 192 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.6% C: 17.7% G: 43.8% T: 10.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 115 419.0 0 115 4 68 104.7 0 68 5 9 26.2 0 9