This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 76.23 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 303,909 (10.7%) Reads that were too short: 9,632 (0.3%) Reads written (passing filters): 294,277 (10.3%) Total basepairs processed: 858,556,748 bp Total written (filtered): 66,934,980 bp (7.8%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 303909 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 738 696.4 0 738 7 1327 696.4 0 1327 8 24032 696.4 0 24032 9 24467 696.4 0 24467 10 61 696.4 0 61 11 58 696.4 0 58 12 49 696.4 0 49 13 41 696.4 0 41 14 40 696.4 0 40 15 42 696.4 0 42 16 34 696.4 0 34 17 50 696.4 0 50 18 68 696.4 0 68 19 63 696.4 0 63 20 68 696.4 0 68 21 50 696.4 0 50 22 70 696.4 0 70 23 80 696.4 0 80 24 84 696.4 0 84 25 31 696.4 0 31 26 52 696.4 0 52 27 51 696.4 0 51 28 54 696.4 0 54 29 377 696.4 0 377 30 121 696.4 0 121 31 54 696.4 0 54 32 57 696.4 0 57 33 62 696.4 0 62 34 49 696.4 0 49 35 63 696.4 0 63 36 105 696.4 0 105 37 97 696.4 0 97 38 151 696.4 0 151 39 100 696.4 0 100 40 96 696.4 0 96 41 56 696.4 0 56 42 122 696.4 0 122 43 95 696.4 0 95 44 95 696.4 0 95 45 149 696.4 0 149 46 222 696.4 0 222 47 353 696.4 0 353 48 662 696.4 0 662 49 1332 696.4 0 1332 50 371 696.4 0 371 51 447 696.4 0 447 52 893 696.4 0 893 53 322 696.4 0 322 54 367 696.4 0 367 55 677 696.4 0 677 56 806 696.4 0 806 57 840 696.4 0 840 58 604 696.4 0 604 59 1305 696.4 0 1305 60 1631 696.4 0 1631 61 2717 696.4 0 2717 62 54239 696.4 0 54239 63 49613 696.4 0 49613 64 51127 696.4 0 51127 65 1736 696.4 0 1736 66 47 696.4 0 47 67 41 696.4 0 41 68 43 696.4 0 43 69 35 696.4 0 35 70 55 696.4 0 55 71 224 696.4 0 224 72 377 696.4 0 377 73 190 696.4 0 190 74 79 696.4 0 79 75 126 696.4 0 126 76 1768 696.4 0 1768 77 1426 696.4 0 1426 78 936 696.4 0 936 79 61 696.4 0 61 80 64 696.4 0 64 81 31 696.4 0 31 82 43 696.4 0 43 83 61 696.4 0 61 84 38 696.4 0 38 85 56 696.4 0 56 86 45 696.4 0 45 87 53 696.4 0 53 88 51 696.4 0 51 89 50 696.4 0 50 90 45 696.4 0 45 91 48 696.4 0 48 92 42 696.4 0 42 93 57 696.4 0 57 94 43 696.4 0 43 95 57 696.4 0 57 96 42 696.4 0 42 97 41 696.4 0 41 98 58 696.4 0 58 99 37 696.4 0 37 100 42 696.4 0 42 101 33 696.4 0 33 102 36 696.4 0 36 103 61 696.4 0 61 104 52 696.4 0 52 105 94 696.4 0 94 106 53 696.4 0 53 107 191 696.4 0 191 108 140 696.4 0 140 109 169 696.4 0 169 110 105 696.4 0 105 111 70 696.4 0 70 112 105 696.4 0 105 113 50 696.4 0 50 114 39 696.4 0 39 115 56 696.4 0 56 116 59 696.4 0 59 117 80 696.4 0 80 118 50 696.4 0 50 119 50 696.4 0 50 120 50 696.4 0 50 121 98 696.4 0 98 122 56 696.4 0 56 123 26 696.4 0 26 124 70 696.4 0 70 125 62 696.4 0 62 126 121 696.4 0 121 127 180 696.4 0 180 128 119 696.4 0 119 129 84 696.4 0 84 130 76 696.4 0 76 131 61 696.4 0 61 132 82 696.4 0 82 133 60 696.4 0 60 134 73 696.4 0 73 135 56 696.4 0 56 136 58 696.4 0 58 137 94 696.4 0 94 138 68 696.4 0 68 139 110 696.4 0 110 140 139 696.4 0 139 141 181 696.4 0 181 142 361 696.4 0 361 143 572 696.4 0 572 144 10082 696.4 0 10082 145 9119 696.4 0 9119 146 5067 696.4 0 5067 147 158 696.4 0 158 148 83 696.4 0 83 149 99 696.4 0 99 150 70 696.4 0 70 151 89 696.4 0 89 152 102 696.4 0 102 153 84 696.4 0 84 154 127 696.4 0 127 155 151 696.4 0 151 156 1235 696.4 0 1235 157 934 696.4 0 934 158 596 696.4 0 596 159 152 696.4 0 152 160 166 696.4 0 166 161 223 696.4 0 223 162 387 696.4 0 387 163 457 696.4 0 457 164 5916 696.4 0 5916 165 5817 696.4 0 5817 166 10686 696.4 0 10686 167 127 696.4 0 127 168 84 696.4 0 84 169 628 696.4 0 628 170 637 696.4 0 637 171 1135 696.4 0 1135 172 995 696.4 0 995 173 384 696.4 0 384 174 250 696.4 0 250 175 79 696.4 0 79 176 68 696.4 0 68 177 58 696.4 0 58 178 63 696.4 0 63 179 148 696.4 0 148 180 145 696.4 0 145 181 128 696.4 0 128 182 69 696.4 0 69 183 61 696.4 0 61 184 125 696.4 0 125 185 93 696.4 0 93 186 125 696.4 0 125 187 61 696.4 0 61 188 85 696.4 0 85 189 458 696.4 0 458 190 629 696.4 0 629 191 570 696.4 0 570 192 273 696.4 0 273 193 50 696.4 0 50 194 31 696.4 0 31 195 37 696.4 0 37 196 90 696.4 0 90 197 58 696.4 0 58 198 81 696.4 0 81 199 67 696.4 0 67 200 61 696.4 0 61 201 39 696.4 0 39 202 52 696.4 0 52 203 65 696.4 0 65 204 65 696.4 0 65 205 54 696.4 0 54 206 54 696.4 0 54 207 47 696.4 0 47 208 62 696.4 0 62 209 69 696.4 0 69 210 48 696.4 0 48 211 50 696.4 0 50 212 70 696.4 0 70 213 69 696.4 0 69 214 79 696.4 0 79 215 111 696.4 0 111 216 127 696.4 0 127 217 168 696.4 0 168 218 136 696.4 0 136 219 59 696.4 0 59 220 46 696.4 0 46 221 39 696.4 0 39 222 39 696.4 0 39 223 47 696.4 0 47 224 76 696.4 0 76 225 233 696.4 0 233 226 107 696.4 0 107 227 142 696.4 0 142 228 102 696.4 0 102 229 548 696.4 0 548 230 329 696.4 0 329 231 143 696.4 0 143 232 62 696.4 0 62 233 72 696.4 0 72 234 50 696.4 0 50 235 42 696.4 0 42 236 81 696.4 0 81 237 96 696.4 0 96 238 101 696.4 0 101 239 62 696.4 0 62 240 53 696.4 0 53 241 55 696.4 0 55 242 33 696.4 0 33 243 63 696.4 0 63 244 74 696.4 0 74 245 64 696.4 0 64 246 61 696.4 0 61 247 78 696.4 0 78 248 55 696.4 0 55 249 52 696.4 0 52 250 50 696.4 0 50 251 53 696.4 0 53 252 56 696.4 0 56 253 40 696.4 0 40 254 46 696.4 0 46 255 49 696.4 0 49 256 36 696.4 0 36 257 47 696.4 0 47 258 48 696.4 0 48 259 59 696.4 0 59 260 122 696.4 0 122 261 161 696.4 0 161 262 168 696.4 0 168 263 129 696.4 0 129 264 81 696.4 0 81 265 81 696.4 0 81 266 95 696.4 0 95 267 95 696.4 0 95 268 80 696.4 0 80 269 50 696.4 0 50 270 57 696.4 0 57 271 84 696.4 0 84 272 57 696.4 0 57 273 78 696.4 0 78 274 80 696.4 0 80 275 72 696.4 0 72 276 58 696.4 0 58 277 144 696.4 0 144 278 119 696.4 0 119 279 77 696.4 0 77 280 77 696.4 0 77 281 85 696.4 0 85 282 47 696.4 0 47 283 58 696.4 0 58 284 65 696.4 0 65 285 126 696.4 0 126 286 161 696.4 0 161 287 518 696.4 0 518 288 457 696.4 0 457 289 306 696.4 0 306 290 133 696.4 0 133 291 66 696.4 0 66 292 59 696.4 0 59 293 71 696.4 0 71 294 55 696.4 0 55 295 127 696.4 0 127 296 115 696.4 0 115 297 72 696.4 0 72 298 76 696.4 0 76 299 66 696.4 0 66 300 58 696.4 0 58 301 72 696.4 0 72 Finished in 13.52 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 294,277 Reads with adapters: 41,742 (14.2%) Reads that were too short: 132 (0.0%) Reads written (passing filters): 41,610 (14.1%) Total basepairs processed: 66,934,980 bp Total written (filtered): 9,730,357 bp (14.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41742 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 11 0.0 3 0 0 10 1 19 276 0.0 3 0 263 11 2 20 26157 0.0 4 25649 487 21 21 56 0.0 4 12 43 1 22 5 0.0 4 0 0 5 23 23 0.0 4 0 0 1 20 2 24 1 0.0 4 0 1 34 44 0.0 4 0 0 0 0 44 35 1 0.0 4 0 0 0 0 1 38 1 0.0 4 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 1 51 1 0.0 4 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 24 0.0 4 0 0 0 0 24 71 31 0.0 4 0 0 0 0 31 72 14680 0.0 4 0 0 0 0 14680 73 1 0.0 4 0 0 0 0 1 75 7 0.0 4 0 0 0 0 7 76 1 0.0 4 0 0 0 0 1 80 3 0.0 4 0 0 0 0 3 85 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 280 0.0 4 0 0 0 0 280 128 2 0.0 4 0 0 0 0 2 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 1 166 4 0.0 4 0 0 0 0 4 167 2 0.0 4 0 0 0 0 2 168 1 0.0 4 0 0 0 1 173 66 0.0 4 0 0 0 1 65 174 9 0.0 4 0 0 0 0 9 175 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 3 0.0 4 0 0 0 1 2 180 7 0.0 4 0 0 0 0 7 181 15 0.0 4 0 0 0 0 15 183 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 227 3 0.0 4 0 0 0 0 3 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 1 280 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.37 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 41,610 Reads with adapters: 5,159 (12.4%) Reads written (passing filters): 41,610 (100.0%) Total basepairs processed: 9,730,357 bp Total written (filtered): 9,681,933 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5159 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.4% C: 2.3% G: 93.0% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 40 650.2 0 40 4 114 162.5 0 114 5 3 40.6 0 3 6 4 10.2 0 4 9 3137 0.2 0 95 3042 10 1557 0.0 1 57 1500 11 290 0.0 1 2 288 12 7 0.0 1 0 7 13 3 0.0 1 0 3 18 1 0.0 1 1 208 2 0.0 1 0 2 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.