This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 76.00 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 151,965 (5.3%) Reads that were too short: 10,277 (0.4%) Reads written (passing filters): 141,688 (5.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 38,131,935 bp (4.4%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 151965 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2764 696.4 0 2764 7 61927 696.4 0 61927 8 49219 696.4 0 49219 9 112 696.4 0 112 10 69 696.4 0 69 11 54 696.4 0 54 12 124 696.4 0 124 13 53 696.4 0 53 14 28 696.4 0 28 15 51 696.4 0 51 16 69 696.4 0 69 17 54 696.4 0 54 18 88 696.4 0 88 19 122 696.4 0 122 20 115 696.4 0 115 21 128 696.4 0 128 22 44 696.4 0 44 23 53 696.4 0 53 24 44 696.4 0 44 25 37 696.4 0 37 26 79 696.4 0 79 27 47 696.4 0 47 28 78 696.4 0 78 29 87 696.4 0 87 30 60 696.4 0 60 31 60 696.4 0 60 32 51 696.4 0 51 33 49 696.4 0 49 34 61 696.4 0 61 35 68 696.4 0 68 36 64 696.4 0 64 37 59 696.4 0 59 38 152 696.4 0 152 39 114 696.4 0 114 40 139 696.4 0 139 41 212 696.4 0 212 42 69 696.4 0 69 43 46 696.4 0 46 44 66 696.4 0 66 45 54 696.4 0 54 46 70 696.4 0 70 47 64 696.4 0 64 48 58 696.4 0 58 49 58 696.4 0 58 50 60 696.4 0 60 51 53 696.4 0 53 52 55 696.4 0 55 53 53 696.4 0 53 54 36 696.4 0 36 55 66 696.4 0 66 56 64 696.4 0 64 57 79 696.4 0 79 58 55 696.4 0 55 59 61 696.4 0 61 60 72 696.4 0 72 61 53 696.4 0 53 62 61 696.4 0 61 63 66 696.4 0 66 64 47 696.4 0 47 65 71 696.4 0 71 66 69 696.4 0 69 67 52 696.4 0 52 68 60 696.4 0 60 69 60 696.4 0 60 70 53 696.4 0 53 71 63 696.4 0 63 72 56 696.4 0 56 73 81 696.4 0 81 74 66 696.4 0 66 75 75 696.4 0 75 76 268 696.4 0 268 77 202 696.4 0 202 78 139 696.4 0 139 79 85 696.4 0 85 80 74 696.4 0 74 81 131 696.4 0 131 82 64 696.4 0 64 83 74 696.4 0 74 84 91 696.4 0 91 85 75 696.4 0 75 86 66 696.4 0 66 87 72 696.4 0 72 88 61 696.4 0 61 89 56 696.4 0 56 90 67 696.4 0 67 91 110 696.4 0 110 92 103 696.4 0 103 93 337 696.4 0 337 94 310 696.4 0 310 95 330 696.4 0 330 96 325 696.4 0 325 97 294 696.4 0 294 98 208 696.4 0 208 99 62 696.4 0 62 100 41 696.4 0 41 101 52 696.4 0 52 102 46 696.4 0 46 103 48 696.4 0 48 104 46 696.4 0 46 105 58 696.4 0 58 106 61 696.4 0 61 107 57 696.4 0 57 108 61 696.4 0 61 109 51 696.4 0 51 110 69 696.4 0 69 111 61 696.4 0 61 112 46 696.4 0 46 113 69 696.4 0 69 114 48 696.4 0 48 115 58 696.4 0 58 116 72 696.4 0 72 117 57 696.4 0 57 118 50 696.4 0 50 119 47 696.4 0 47 120 37 696.4 0 37 121 41 696.4 0 41 122 62 696.4 0 62 123 44 696.4 0 44 124 43 696.4 0 43 125 52 696.4 0 52 126 43 696.4 0 43 127 61 696.4 0 61 128 67 696.4 0 67 129 123 696.4 0 123 130 166 696.4 0 166 131 423 696.4 0 423 132 282 696.4 0 282 133 118 696.4 0 118 134 133 696.4 0 133 135 112 696.4 0 112 136 105 696.4 0 105 137 72 696.4 0 72 138 63 696.4 0 63 139 59 696.4 0 59 140 60 696.4 0 60 141 46 696.4 0 46 142 69 696.4 0 69 143 93 696.4 0 93 144 122 696.4 0 122 145 112 696.4 0 112 146 52 696.4 0 52 147 48 696.4 0 48 148 58 696.4 0 58 149 53 696.4 0 53 150 60 696.4 0 60 151 82 696.4 0 82 152 81 696.4 0 81 153 58 696.4 0 58 154 74 696.4 0 74 155 56 696.4 0 56 156 79 696.4 0 79 157 73 696.4 0 73 158 63 696.4 0 63 159 58 696.4 0 58 160 51 696.4 0 51 161 59 696.4 0 59 162 72 696.4 0 72 163 57 696.4 0 57 164 88 696.4 0 88 165 114 696.4 0 114 166 136 696.4 0 136 167 280 696.4 0 280 168 278 696.4 0 278 169 2857 696.4 0 2857 170 2929 696.4 0 2929 171 6242 696.4 0 6242 172 88 696.4 0 88 173 53 696.4 0 53 174 92 696.4 0 92 175 50 696.4 0 50 176 44 696.4 0 44 177 35 696.4 0 35 178 46 696.4 0 46 179 55 696.4 0 55 180 60 696.4 0 60 181 67 696.4 0 67 182 50 696.4 0 50 183 52 696.4 0 52 184 47 696.4 0 47 185 64 696.4 0 64 186 64 696.4 0 64 187 39 696.4 0 39 188 49 696.4 0 49 189 54 696.4 0 54 190 57 696.4 0 57 191 48 696.4 0 48 192 71 696.4 0 71 193 58 696.4 0 58 194 86 696.4 0 86 195 46 696.4 0 46 196 59 696.4 0 59 197 47 696.4 0 47 198 64 696.4 0 64 199 55 696.4 0 55 200 54 696.4 0 54 201 59 696.4 0 59 202 67 696.4 0 67 203 138 696.4 0 138 204 115 696.4 0 115 205 93 696.4 0 93 206 74 696.4 0 74 207 46 696.4 0 46 208 46 696.4 0 46 209 68 696.4 0 68 210 82 696.4 0 82 211 70 696.4 0 70 212 67 696.4 0 67 213 87 696.4 0 87 214 56 696.4 0 56 215 75 696.4 0 75 216 75 696.4 0 75 217 111 696.4 0 111 218 87 696.4 0 87 219 71 696.4 0 71 220 39 696.4 0 39 221 68 696.4 0 68 222 105 696.4 0 105 223 76 696.4 0 76 224 104 696.4 0 104 225 50 696.4 0 50 226 52 696.4 0 52 227 53 696.4 0 53 228 63 696.4 0 63 229 54 696.4 0 54 230 146 696.4 0 146 231 103 696.4 0 103 232 197 696.4 0 197 233 105 696.4 0 105 234 101 696.4 0 101 235 180 696.4 0 180 236 234 696.4 0 234 237 249 696.4 0 249 238 183 696.4 0 183 239 71 696.4 0 71 240 77 696.4 0 77 241 71 696.4 0 71 242 99 696.4 0 99 243 113 696.4 0 113 244 53 696.4 0 53 245 43 696.4 0 43 246 82 696.4 0 82 247 50 696.4 0 50 248 51 696.4 0 51 249 71 696.4 0 71 250 89 696.4 0 89 251 68 696.4 0 68 252 63 696.4 0 63 253 65 696.4 0 65 254 135 696.4 0 135 255 116 696.4 0 116 256 127 696.4 0 127 257 64 696.4 0 64 258 79 696.4 0 79 259 51 696.4 0 51 260 61 696.4 0 61 261 85 696.4 0 85 262 125 696.4 0 125 263 103 696.4 0 103 264 84 696.4 0 84 265 66 696.4 0 66 266 80 696.4 0 80 267 59 696.4 0 59 268 56 696.4 0 56 269 69 696.4 0 69 270 264 696.4 0 264 271 250 696.4 0 250 272 264 696.4 0 264 273 177 696.4 0 177 274 202 696.4 0 202 275 60 696.4 0 60 276 74 696.4 0 74 277 121 696.4 0 121 278 163 696.4 0 163 279 146 696.4 0 146 280 87 696.4 0 87 281 81 696.4 0 81 282 53 696.4 0 53 283 93 696.4 0 93 284 71 696.4 0 71 285 89 696.4 0 89 286 72 696.4 0 72 287 57 696.4 0 57 288 54 696.4 0 54 289 68 696.4 0 68 290 76 696.4 0 76 291 71 696.4 0 71 292 117 696.4 0 117 293 96 696.4 0 96 294 139 696.4 0 139 295 130 696.4 0 130 296 288 696.4 0 288 297 334 696.4 0 334 298 282 696.4 0 282 299 132 696.4 0 132 300 117 696.4 0 117 301 33 696.4 0 33 Finished in 6.72 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 141,688 Reads with adapters: 59,695 (42.1%) Reads that were too short: 70 (0.0%) Reads written (passing filters): 59,625 (42.1%) Total basepairs processed: 38,131,935 bp Total written (filtered): 16,504,223 bp (43.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 59695 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 33 0.0 3 0 0 18 15 16 600 0.0 3 0 507 57 36 17 58307 0.0 3 54828 2915 467 97 18 142 0.0 3 7 112 13 10 19 96 0.0 3 1 1 51 43 20 360 0.0 3 1 0 0 359 21 2 0.0 3 1 1 22 1 0.0 3 1 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 1 44 12 0.0 3 0 0 0 12 47 7 0.0 3 0 0 1 6 48 1 0.0 3 0 0 0 1 50 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 16 0.0 3 0 0 0 16 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 1 79 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 96 2 0.0 3 1 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 5 0.0 3 0 0 0 5 101 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 168 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 3 0.0 3 0 0 0 3 194 9 0.0 3 0 0 1 8 198 1 0.0 3 0 0 1 202 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 12 0.0 3 0 0 0 12 282 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.11 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 59,625 Reads with adapters: 472 (0.8%) Reads written (passing filters): 59,625 (100.0%) Total basepairs processed: 16,504,223 bp Total written (filtered): 16,502,729 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 472 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.8% C: 59.1% G: 12.5% T: 7.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 443 931.6 0 443 4 7 232.9 0 7 5 14 58.2 0 14 6 4 14.6 0 4 9 1 0.2 0 0 1 10 1 0.1 1 0 1 12 2 0.0 1 0 2