This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.59 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 156,532 (5.5%) Reads that were too short: 8,544 (0.3%) Reads written (passing filters): 147,988 (5.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 39,547,968 bp (4.6%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 156532 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2475 696.4 0 2475 7 58851 696.4 0 58851 8 57179 696.4 0 57179 9 117 696.4 0 117 10 64 696.4 0 64 11 55 696.4 0 55 12 77 696.4 0 77 13 34 696.4 0 34 14 50 696.4 0 50 15 75 696.4 0 75 16 50 696.4 0 50 17 40 696.4 0 40 18 66 696.4 0 66 19 106 696.4 0 106 20 91 696.4 0 91 21 92 696.4 0 92 22 52 696.4 0 52 23 64 696.4 0 64 24 35 696.4 0 35 25 61 696.4 0 61 26 65 696.4 0 65 27 66 696.4 0 66 28 50 696.4 0 50 29 50 696.4 0 50 30 63 696.4 0 63 31 59 696.4 0 59 32 47 696.4 0 47 33 41 696.4 0 41 34 69 696.4 0 69 35 68 696.4 0 68 36 70 696.4 0 70 37 61 696.4 0 61 38 142 696.4 0 142 39 83 696.4 0 83 40 78 696.4 0 78 41 61 696.4 0 61 42 54 696.4 0 54 43 52 696.4 0 52 44 56 696.4 0 56 45 72 696.4 0 72 46 77 696.4 0 77 47 49 696.4 0 49 48 52 696.4 0 52 49 62 696.4 0 62 50 62 696.4 0 62 51 61 696.4 0 61 52 66 696.4 0 66 53 74 696.4 0 74 54 60 696.4 0 60 55 62 696.4 0 62 56 61 696.4 0 61 57 64 696.4 0 64 58 76 696.4 0 76 59 78 696.4 0 78 60 66 696.4 0 66 61 53 696.4 0 53 62 64 696.4 0 64 63 51 696.4 0 51 64 51 696.4 0 51 65 51 696.4 0 51 66 75 696.4 0 75 67 39 696.4 0 39 68 68 696.4 0 68 69 58 696.4 0 58 70 59 696.4 0 59 71 66 696.4 0 66 72 56 696.4 0 56 73 66 696.4 0 66 74 53 696.4 0 53 75 70 696.4 0 70 76 248 696.4 0 248 77 189 696.4 0 189 78 128 696.4 0 128 79 77 696.4 0 77 80 65 696.4 0 65 81 84 696.4 0 84 82 54 696.4 0 54 83 63 696.4 0 63 84 58 696.4 0 58 85 60 696.4 0 60 86 53 696.4 0 53 87 65 696.4 0 65 88 77 696.4 0 77 89 49 696.4 0 49 90 75 696.4 0 75 91 83 696.4 0 83 92 74 696.4 0 74 93 170 696.4 0 170 94 155 696.4 0 155 95 171 696.4 0 171 96 145 696.4 0 145 97 157 696.4 0 157 98 106 696.4 0 106 99 42 696.4 0 42 100 51 696.4 0 51 101 56 696.4 0 56 102 56 696.4 0 56 103 44 696.4 0 44 104 45 696.4 0 45 105 56 696.4 0 56 106 47 696.4 0 47 107 57 696.4 0 57 108 57 696.4 0 57 109 42 696.4 0 42 110 52 696.4 0 52 111 36 696.4 0 36 112 36 696.4 0 36 113 91 696.4 0 91 114 71 696.4 0 71 115 54 696.4 0 54 116 74 696.4 0 74 117 64 696.4 0 64 118 34 696.4 0 34 119 44 696.4 0 44 120 32 696.4 0 32 121 39 696.4 0 39 122 54 696.4 0 54 123 52 696.4 0 52 124 37 696.4 0 37 125 52 696.4 0 52 126 44 696.4 0 44 127 54 696.4 0 54 128 66 696.4 0 66 129 93 696.4 0 93 130 89 696.4 0 89 131 439 696.4 0 439 132 284 696.4 0 284 133 117 696.4 0 117 134 205 696.4 0 205 135 139 696.4 0 139 136 132 696.4 0 132 137 84 696.4 0 84 138 72 696.4 0 72 139 63 696.4 0 63 140 47 696.4 0 47 141 65 696.4 0 65 142 64 696.4 0 64 143 87 696.4 0 87 144 83 696.4 0 83 145 85 696.4 0 85 146 47 696.4 0 47 147 37 696.4 0 37 148 42 696.4 0 42 149 56 696.4 0 56 150 54 696.4 0 54 151 78 696.4 0 78 152 93 696.4 0 93 153 51 696.4 0 51 154 74 696.4 0 74 155 59 696.4 0 59 156 54 696.4 0 54 157 66 696.4 0 66 158 69 696.4 0 69 159 74 696.4 0 74 160 59 696.4 0 59 161 49 696.4 0 49 162 70 696.4 0 70 163 74 696.4 0 74 164 91 696.4 0 91 165 129 696.4 0 129 166 164 696.4 0 164 167 301 696.4 0 301 168 265 696.4 0 265 169 3569 696.4 0 3569 170 3948 696.4 0 3948 171 7778 696.4 0 7778 172 80 696.4 0 80 173 58 696.4 0 58 174 87 696.4 0 87 175 61 696.4 0 61 176 45 696.4 0 45 177 52 696.4 0 52 178 44 696.4 0 44 179 64 696.4 0 64 180 57 696.4 0 57 181 56 696.4 0 56 182 58 696.4 0 58 183 59 696.4 0 59 184 56 696.4 0 56 185 59 696.4 0 59 186 54 696.4 0 54 187 55 696.4 0 55 188 54 696.4 0 54 189 53 696.4 0 53 190 54 696.4 0 54 191 58 696.4 0 58 192 76 696.4 0 76 193 52 696.4 0 52 194 106 696.4 0 106 195 54 696.4 0 54 196 48 696.4 0 48 197 39 696.4 0 39 198 57 696.4 0 57 199 57 696.4 0 57 200 39 696.4 0 39 201 61 696.4 0 61 202 68 696.4 0 68 203 109 696.4 0 109 204 82 696.4 0 82 205 96 696.4 0 96 206 67 696.4 0 67 207 46 696.4 0 46 208 47 696.4 0 47 209 58 696.4 0 58 210 56 696.4 0 56 211 55 696.4 0 55 212 47 696.4 0 47 213 57 696.4 0 57 214 26 696.4 0 26 215 50 696.4 0 50 216 60 696.4 0 60 217 86 696.4 0 86 218 78 696.4 0 78 219 54 696.4 0 54 220 39 696.4 0 39 221 69 696.4 0 69 222 111 696.4 0 111 223 81 696.4 0 81 224 99 696.4 0 99 225 38 696.4 0 38 226 69 696.4 0 69 227 61 696.4 0 61 228 69 696.4 0 69 229 55 696.4 0 55 230 152 696.4 0 152 231 90 696.4 0 90 232 183 696.4 0 183 233 69 696.4 0 69 234 58 696.4 0 58 235 76 696.4 0 76 236 153 696.4 0 153 237 126 696.4 0 126 238 109 696.4 0 109 239 54 696.4 0 54 240 57 696.4 0 57 241 53 696.4 0 53 242 85 696.4 0 85 243 46 696.4 0 46 244 49 696.4 0 49 245 65 696.4 0 65 246 86 696.4 0 86 247 49 696.4 0 49 248 41 696.4 0 41 249 50 696.4 0 50 250 53 696.4 0 53 251 44 696.4 0 44 252 47 696.4 0 47 253 80 696.4 0 80 254 80 696.4 0 80 255 55 696.4 0 55 256 64 696.4 0 64 257 44 696.4 0 44 258 66 696.4 0 66 259 62 696.4 0 62 260 54 696.4 0 54 261 62 696.4 0 62 262 105 696.4 0 105 263 74 696.4 0 74 264 59 696.4 0 59 265 63 696.4 0 63 266 68 696.4 0 68 267 71 696.4 0 71 268 63 696.4 0 63 269 70 696.4 0 70 270 262 696.4 0 262 271 283 696.4 0 283 272 349 696.4 0 349 273 267 696.4 0 267 274 308 696.4 0 308 275 36 696.4 0 36 276 55 696.4 0 55 277 83 696.4 0 83 278 142 696.4 0 142 279 111 696.4 0 111 280 68 696.4 0 68 281 62 696.4 0 62 282 48 696.4 0 48 283 86 696.4 0 86 284 55 696.4 0 55 285 62 696.4 0 62 286 52 696.4 0 52 287 59 696.4 0 59 288 57 696.4 0 57 289 61 696.4 0 61 290 68 696.4 0 68 291 77 696.4 0 77 292 101 696.4 0 101 293 68 696.4 0 68 294 116 696.4 0 116 295 82 696.4 0 82 296 191 696.4 0 191 297 207 696.4 0 207 298 177 696.4 0 177 299 73 696.4 0 73 300 67 696.4 0 67 301 43 696.4 0 43 Finished in 7.49 s (51 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 147,988 Reads with adapters: 63,333 (42.8%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 63,313 (42.8%) Total basepairs processed: 39,547,968 bp Total written (filtered): 17,244,494 bp (43.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63333 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 2 7 18 17 0.0 3 0 0 14 1 2 19 609 0.0 3 0 591 18 20 61344 0.0 4 60209 1089 40 4 2 21 143 0.0 4 26 110 6 1 22 24 0.0 4 1 9 14 23 738 0.0 4 669 13 2 45 9 24 4 0.0 4 2 1 0 0 1 25 1 0.0 4 1 31 1 0.0 4 1 35 1 0.0 4 1 41 42 0.0 4 0 0 0 0 42 42 3 0.0 4 0 0 0 0 3 54 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 96 9 0.0 4 0 0 0 0 9 104 1 0.0 4 0 0 1 114 1 0.0 4 1 115 1 0.0 4 0 1 124 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 353 0.0 4 0 0 0 0 353 128 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 184 3 0.0 4 0 0 0 0 3 191 1 0.0 4 0 0 1 226 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 237 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.21 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 63,313 Reads with adapters: 3,938 (6.2%) Reads written (passing filters): 63,313 (100.0%) Total basepairs processed: 17,244,494 bp Total written (filtered): 17,199,384 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3938 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.4% C: 4.4% G: 91.6% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 53 989.3 0 53 4 4 247.3 0 4 9 157 0.2 0 9 148 10 1012 0.1 1 50 962 11 2673 0.0 1 22 2651 12 14 0.0 1 4 10 13 5 0.0 1 1 4 14 1 0.0 1 0 1 15 1 0.0 1 0 1 72 1 0.0 1 0 1 139 1 0.0 1 1 151 2 0.0 1 0 2 209 5 0.0 1 1 4 210 1 0.0 1 0 1 224 4 0.0 1 3 1 261 1 0.0 1 1 270 2 0.0 1 2 272 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.