This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.10 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 99,858 (3.5%) Reads that were too short: 8,978 (0.3%) Reads written (passing filters): 90,880 (3.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 25,167,591 bp (2.9%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 99858 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 995 696.4 0 995 7 1203 696.4 0 1203 8 43241 696.4 0 43241 9 33103 696.4 0 33103 10 83 696.4 0 83 11 88 696.4 0 88 12 14 696.4 0 14 13 27 696.4 0 27 14 21 696.4 0 21 15 31 696.4 0 31 16 18 696.4 0 18 17 8 696.4 0 8 18 19 696.4 0 19 19 17 696.4 0 17 20 18 696.4 0 18 21 34 696.4 0 34 22 29 696.4 0 29 23 17 696.4 0 17 24 14 696.4 0 14 25 16 696.4 0 16 26 11 696.4 0 11 27 20 696.4 0 20 28 16 696.4 0 16 29 8 696.4 0 8 30 21 696.4 0 21 31 24 696.4 0 24 32 7 696.4 0 7 33 34 696.4 0 34 34 20 696.4 0 20 35 52 696.4 0 52 36 21 696.4 0 21 37 35 696.4 0 35 38 45 696.4 0 45 39 15 696.4 0 15 40 16 696.4 0 16 41 41 696.4 0 41 42 9 696.4 0 9 43 24 696.4 0 24 44 21 696.4 0 21 45 22 696.4 0 22 46 34 696.4 0 34 47 364 696.4 0 364 48 411 696.4 0 411 49 363 696.4 0 363 50 696 696.4 0 696 51 50 696.4 0 50 52 15 696.4 0 15 53 11 696.4 0 11 54 10 696.4 0 10 55 12 696.4 0 12 56 16 696.4 0 16 57 13 696.4 0 13 58 8 696.4 0 8 59 18 696.4 0 18 60 19 696.4 0 19 61 11 696.4 0 11 62 31 696.4 0 31 63 9 696.4 0 9 64 10 696.4 0 10 65 15 696.4 0 15 66 7 696.4 0 7 67 16 696.4 0 16 68 4 696.4 0 4 69 10 696.4 0 10 70 11 696.4 0 11 71 14 696.4 0 14 72 7 696.4 0 7 73 14 696.4 0 14 74 25 696.4 0 25 75 22 696.4 0 22 76 20 696.4 0 20 77 17 696.4 0 17 78 31 696.4 0 31 79 11 696.4 0 11 80 28 696.4 0 28 81 77 696.4 0 77 82 118 696.4 0 118 83 81 696.4 0 81 84 57 696.4 0 57 85 26 696.4 0 26 86 23 696.4 0 23 87 186 696.4 0 186 88 45 696.4 0 45 89 38 696.4 0 38 90 100 696.4 0 100 91 36 696.4 0 36 92 17 696.4 0 17 93 32 696.4 0 32 94 15 696.4 0 15 95 20 696.4 0 20 96 36 696.4 0 36 97 81 696.4 0 81 98 88 696.4 0 88 99 15 696.4 0 15 100 11 696.4 0 11 101 10 696.4 0 10 102 19 696.4 0 19 103 14 696.4 0 14 104 19 696.4 0 19 105 32 696.4 0 32 106 15 696.4 0 15 107 33 696.4 0 33 108 26 696.4 0 26 109 18 696.4 0 18 110 22 696.4 0 22 111 22 696.4 0 22 112 23 696.4 0 23 113 74 696.4 0 74 114 48 696.4 0 48 115 94 696.4 0 94 116 27 696.4 0 27 117 57 696.4 0 57 118 234 696.4 0 234 119 135 696.4 0 135 120 257 696.4 0 257 121 21 696.4 0 21 122 27 696.4 0 27 123 15 696.4 0 15 124 14 696.4 0 14 125 37 696.4 0 37 126 19 696.4 0 19 127 21 696.4 0 21 128 26 696.4 0 26 129 15 696.4 0 15 130 23 696.4 0 23 131 29 696.4 0 29 132 34 696.4 0 34 133 65 696.4 0 65 134 35 696.4 0 35 135 37 696.4 0 37 136 219 696.4 0 219 137 139 696.4 0 139 138 304 696.4 0 304 139 41 696.4 0 41 140 45 696.4 0 45 141 51 696.4 0 51 142 98 696.4 0 98 143 63 696.4 0 63 144 98 696.4 0 98 145 74 696.4 0 74 146 213 696.4 0 213 147 104 696.4 0 104 148 858 696.4 0 858 149 38 696.4 0 38 150 34 696.4 0 34 151 18 696.4 0 18 152 26 696.4 0 26 153 41 696.4 0 41 154 33 696.4 0 33 155 28 696.4 0 28 156 35 696.4 0 35 157 15 696.4 0 15 158 33 696.4 0 33 159 23 696.4 0 23 160 16 696.4 0 16 161 23 696.4 0 23 162 26 696.4 0 26 163 15 696.4 0 15 164 17 696.4 0 17 165 29 696.4 0 29 166 28 696.4 0 28 167 23 696.4 0 23 168 24 696.4 0 24 169 29 696.4 0 29 170 20 696.4 0 20 171 24 696.4 0 24 172 23 696.4 0 23 173 11 696.4 0 11 174 16 696.4 0 16 175 18 696.4 0 18 176 33 696.4 0 33 177 31 696.4 0 31 178 26 696.4 0 26 179 26 696.4 0 26 180 28 696.4 0 28 181 42 696.4 0 42 182 66 696.4 0 66 183 165 696.4 0 165 184 34 696.4 0 34 185 15 696.4 0 15 186 11 696.4 0 11 187 21 696.4 0 21 188 31 696.4 0 31 189 57 696.4 0 57 190 61 696.4 0 61 191 68 696.4 0 68 192 71 696.4 0 71 193 85 696.4 0 85 194 193 696.4 0 193 195 793 696.4 0 793 196 827 696.4 0 827 197 571 696.4 0 571 198 53 696.4 0 53 199 35 696.4 0 35 200 17 696.4 0 17 201 37 696.4 0 37 202 28 696.4 0 28 203 28 696.4 0 28 204 29 696.4 0 29 205 27 696.4 0 27 206 21 696.4 0 21 207 30 696.4 0 30 208 25 696.4 0 25 209 16 696.4 0 16 210 25 696.4 0 25 211 22 696.4 0 22 212 46 696.4 0 46 213 66 696.4 0 66 214 111 696.4 0 111 215 101 696.4 0 101 216 92 696.4 0 92 217 54 696.4 0 54 218 56 696.4 0 56 219 39 696.4 0 39 220 61 696.4 0 61 221 67 696.4 0 67 222 30 696.4 0 30 223 27 696.4 0 27 224 33 696.4 0 33 225 31 696.4 0 31 226 23 696.4 0 23 227 28 696.4 0 28 228 23 696.4 0 23 229 61 696.4 0 61 230 53 696.4 0 53 231 108 696.4 0 108 232 64 696.4 0 64 233 42 696.4 0 42 234 100 696.4 0 100 235 60 696.4 0 60 236 59 696.4 0 59 237 42 696.4 0 42 238 53 696.4 0 53 239 82 696.4 0 82 240 100 696.4 0 100 241 85 696.4 0 85 242 54 696.4 0 54 243 361 696.4 0 361 244 242 696.4 0 242 245 372 696.4 0 372 246 193 696.4 0 193 247 51 696.4 0 51 248 62 696.4 0 62 249 82 696.4 0 82 250 111 696.4 0 111 251 119 696.4 0 119 252 119 696.4 0 119 253 75 696.4 0 75 254 84 696.4 0 84 255 49 696.4 0 49 256 59 696.4 0 59 257 62 696.4 0 62 258 94 696.4 0 94 259 120 696.4 0 120 260 119 696.4 0 119 261 374 696.4 0 374 262 157 696.4 0 157 263 266 696.4 0 266 264 58 696.4 0 58 265 38 696.4 0 38 266 45 696.4 0 45 267 22 696.4 0 22 268 69 696.4 0 69 269 133 696.4 0 133 270 193 696.4 0 193 271 141 696.4 0 141 272 39 696.4 0 39 273 27 696.4 0 27 274 35 696.4 0 35 275 40 696.4 0 40 276 31 696.4 0 31 277 54 696.4 0 54 278 42 696.4 0 42 279 44 696.4 0 44 280 59 696.4 0 59 281 89 696.4 0 89 282 93 696.4 0 93 283 78 696.4 0 78 284 94 696.4 0 94 285 84 696.4 0 84 286 79 696.4 0 79 287 112 696.4 0 112 288 123 696.4 0 123 289 322 696.4 0 322 290 155 696.4 0 155 291 142 696.4 0 142 292 91 696.4 0 91 293 112 696.4 0 112 294 80 696.4 0 80 295 66 696.4 0 66 296 107 696.4 0 107 297 243 696.4 0 243 298 190 696.4 0 190 299 223 696.4 0 223 300 74 696.4 0 74 301 78 696.4 0 78 Finished in 4.74 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 90,880 Reads with adapters: 40,876 (45.0%) Reads that were too short: 111 (0.1%) Reads written (passing filters): 40,765 (44.9%) Total basepairs processed: 25,167,591 bp Total written (filtered): 11,228,270 bp (44.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 40876 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 18 0.0 3 0 0 13 5 16 350 0.0 3 0 295 38 17 17 39818 0.0 3 37332 2062 341 83 18 130 0.0 3 3 100 16 11 19 97 0.0 3 14 6 51 26 20 190 0.0 3 0 1 0 189 22 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 28 3 0.0 3 0 0 1 2 29 8 0.0 3 0 0 0 8 31 5 0.0 3 0 0 0 5 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 1 35 24 0.0 3 0 0 0 24 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 4 0.0 3 0 0 0 4 42 4 0.0 3 0 0 0 4 44 1 0.0 3 0 0 0 1 45 27 0.0 3 0 0 0 27 48 1 0.0 3 0 0 0 1 53 9 0.0 3 0 0 0 9 54 7 0.0 3 0 0 0 7 55 4 0.0 3 0 0 0 4 56 5 0.0 3 0 0 1 4 61 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 92 4 0.0 3 0 0 1 3 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 7 0.0 3 0 0 0 7 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 142 1 0.0 3 0 0 0 1 143 18 0.0 3 0 0 0 18 144 4 0.0 3 0 0 0 4 147 6 0.0 3 0 0 0 6 149 2 0.0 3 0 0 0 2 150 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 1 1 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 5 0.0 3 0 0 0 5 167 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 193 1 0.0 3 0 0 0 1 194 6 0.0 3 0 0 0 6 205 3 0.0 3 0 0 0 3 206 2 0.0 3 0 0 0 2 207 1 0.0 3 0 0 1 211 1 0.0 3 0 0 0 1 217 8 0.0 3 0 0 0 8 218 1 0.0 3 0 0 1 221 4 0.0 3 0 0 0 4 239 2 0.0 3 0 0 0 2 244 5 0.0 3 0 0 0 5 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 7 0.0 3 0 0 0 7 281 7 0.0 3 0 0 0 7 282 5 0.0 3 0 0 0 5 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.45 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 40,765 Reads with adapters: 225 (0.6%) Reads written (passing filters): 40,765 (100.0%) Total basepairs processed: 11,228,270 bp Total written (filtered): 11,227,553 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 225 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.4% C: 13.8% G: 36.4% T: 13.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 193 637.0 0 193 4 24 159.2 0 24 5 7 39.8 0 7 7 1 2.5 0 1