This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.37 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 91,001 (3.2%) Reads that were too short: 3,434 (0.1%) Reads written (passing filters): 87,567 (3.1%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,901,128 bp (2.9%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 91001 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 932 696.4 0 932 7 1014 696.4 0 1014 8 41276 696.4 0 41276 9 38396 696.4 0 38396 10 71 696.4 0 71 11 26 696.4 0 26 12 15 696.4 0 15 13 8 696.4 0 8 14 7 696.4 0 7 15 6 696.4 0 6 16 3 696.4 0 3 17 8 696.4 0 8 18 6 696.4 0 6 19 9 696.4 0 9 20 5 696.4 0 5 21 4 696.4 0 4 22 8 696.4 0 8 23 13 696.4 0 13 24 8 696.4 0 8 25 4 696.4 0 4 26 5 696.4 0 5 27 12 696.4 0 12 28 8 696.4 0 8 29 15 696.4 0 15 30 13 696.4 0 13 31 15 696.4 0 15 32 7 696.4 0 7 33 6 696.4 0 6 34 9 696.4 0 9 35 15 696.4 0 15 36 8 696.4 0 8 37 20 696.4 0 20 38 24 696.4 0 24 39 13 696.4 0 13 40 16 696.4 0 16 41 15 696.4 0 15 42 7 696.4 0 7 43 12 696.4 0 12 44 8 696.4 0 8 45 11 696.4 0 11 46 11 696.4 0 11 47 26 696.4 0 26 48 179 696.4 0 179 49 115 696.4 0 115 50 252 696.4 0 252 51 12 696.4 0 12 52 9 696.4 0 9 53 11 696.4 0 11 54 6 696.4 0 6 55 16 696.4 0 16 56 11 696.4 0 11 57 7 696.4 0 7 58 3 696.4 0 3 59 4 696.4 0 4 60 11 696.4 0 11 61 9 696.4 0 9 62 12 696.4 0 12 63 14 696.4 0 14 64 11 696.4 0 11 65 8 696.4 0 8 66 4 696.4 0 4 67 9 696.4 0 9 68 6 696.4 0 6 69 12 696.4 0 12 70 8 696.4 0 8 71 10 696.4 0 10 72 7 696.4 0 7 73 9 696.4 0 9 74 9 696.4 0 9 75 11 696.4 0 11 76 9 696.4 0 9 77 10 696.4 0 10 78 9 696.4 0 9 79 9 696.4 0 9 80 16 696.4 0 16 81 21 696.4 0 21 82 33 696.4 0 33 83 40 696.4 0 40 84 27 696.4 0 27 85 24 696.4 0 24 86 11 696.4 0 11 87 14 696.4 0 14 88 9 696.4 0 9 89 19 696.4 0 19 90 14 696.4 0 14 91 5 696.4 0 5 92 12 696.4 0 12 93 6 696.4 0 6 94 8 696.4 0 8 95 7 696.4 0 7 96 14 696.4 0 14 97 43 696.4 0 43 98 32 696.4 0 32 99 10 696.4 0 10 100 5 696.4 0 5 101 9 696.4 0 9 102 10 696.4 0 10 103 10 696.4 0 10 104 15 696.4 0 15 105 22 696.4 0 22 106 14 696.4 0 14 107 13 696.4 0 13 108 21 696.4 0 21 109 18 696.4 0 18 110 6 696.4 0 6 111 13 696.4 0 13 112 6 696.4 0 6 113 25 696.4 0 25 114 17 696.4 0 17 115 21 696.4 0 21 116 10 696.4 0 10 117 23 696.4 0 23 118 108 696.4 0 108 119 67 696.4 0 67 120 132 696.4 0 132 121 22 696.4 0 22 122 19 696.4 0 19 123 13 696.4 0 13 124 11 696.4 0 11 125 21 696.4 0 21 126 19 696.4 0 19 127 21 696.4 0 21 128 10 696.4 0 10 129 15 696.4 0 15 130 16 696.4 0 16 131 14 696.4 0 14 132 23 696.4 0 23 133 44 696.4 0 44 134 18 696.4 0 18 135 26 696.4 0 26 136 69 696.4 0 69 137 46 696.4 0 46 138 77 696.4 0 77 139 21 696.4 0 21 140 21 696.4 0 21 141 28 696.4 0 28 142 32 696.4 0 32 143 34 696.4 0 34 144 21 696.4 0 21 145 29 696.4 0 29 146 201 696.4 0 201 147 79 696.4 0 79 148 876 696.4 0 876 149 18 696.4 0 18 150 17 696.4 0 17 151 16 696.4 0 16 152 19 696.4 0 19 153 22 696.4 0 22 154 30 696.4 0 30 155 8 696.4 0 8 156 20 696.4 0 20 157 16 696.4 0 16 158 19 696.4 0 19 159 9 696.4 0 9 160 9 696.4 0 9 161 9 696.4 0 9 162 10 696.4 0 10 163 11 696.4 0 11 164 12 696.4 0 12 165 20 696.4 0 20 166 15 696.4 0 15 167 13 696.4 0 13 168 13 696.4 0 13 169 9 696.4 0 9 170 9 696.4 0 9 171 18 696.4 0 18 172 9 696.4 0 9 173 15 696.4 0 15 174 10 696.4 0 10 175 12 696.4 0 12 176 15 696.4 0 15 177 15 696.4 0 15 178 14 696.4 0 14 179 29 696.4 0 29 180 14 696.4 0 14 181 49 696.4 0 49 182 54 696.4 0 54 183 164 696.4 0 164 184 9 696.4 0 9 185 7 696.4 0 7 186 15 696.4 0 15 187 11 696.4 0 11 188 23 696.4 0 23 189 31 696.4 0 31 190 25 696.4 0 25 191 67 696.4 0 67 192 30 696.4 0 30 193 38 696.4 0 38 194 58 696.4 0 58 195 315 696.4 0 315 196 269 696.4 0 269 197 219 696.4 0 219 198 13 696.4 0 13 199 15 696.4 0 15 200 7 696.4 0 7 201 10 696.4 0 10 202 12 696.4 0 12 203 15 696.4 0 15 204 8 696.4 0 8 205 11 696.4 0 11 206 18 696.4 0 18 207 7 696.4 0 7 208 8 696.4 0 8 209 16 696.4 0 16 210 12 696.4 0 12 211 8 696.4 0 8 212 11 696.4 0 11 213 16 696.4 0 16 214 31 696.4 0 31 215 26 696.4 0 26 216 57 696.4 0 57 217 25 696.4 0 25 218 42 696.4 0 42 219 12 696.4 0 12 220 21 696.4 0 21 221 26 696.4 0 26 222 13 696.4 0 13 223 13 696.4 0 13 224 29 696.4 0 29 225 16 696.4 0 16 226 15 696.4 0 15 227 9 696.4 0 9 228 6 696.4 0 6 229 21 696.4 0 21 230 32 696.4 0 32 231 29 696.4 0 29 232 31 696.4 0 31 233 14 696.4 0 14 234 78 696.4 0 78 235 51 696.4 0 51 236 19 696.4 0 19 237 19 696.4 0 19 238 27 696.4 0 27 239 39 696.4 0 39 240 30 696.4 0 30 241 24 696.4 0 24 242 23 696.4 0 23 243 116 696.4 0 116 244 90 696.4 0 90 245 113 696.4 0 113 246 47 696.4 0 47 247 17 696.4 0 17 248 23 696.4 0 23 249 26 696.4 0 26 250 42 696.4 0 42 251 52 696.4 0 52 252 48 696.4 0 48 253 37 696.4 0 37 254 20 696.4 0 20 255 16 696.4 0 16 256 31 696.4 0 31 257 29 696.4 0 29 258 55 696.4 0 55 259 62 696.4 0 62 260 78 696.4 0 78 261 126 696.4 0 126 262 51 696.4 0 51 263 129 696.4 0 129 264 19 696.4 0 19 265 18 696.4 0 18 266 18 696.4 0 18 267 13 696.4 0 13 268 11 696.4 0 11 269 33 696.4 0 33 270 52 696.4 0 52 271 37 696.4 0 37 272 20 696.4 0 20 273 14 696.4 0 14 274 18 696.4 0 18 275 18 696.4 0 18 276 11 696.4 0 11 277 12 696.4 0 12 278 17 696.4 0 17 279 14 696.4 0 14 280 15 696.4 0 15 281 27 696.4 0 27 282 41 696.4 0 41 283 17 696.4 0 17 284 27 696.4 0 27 285 31 696.4 0 31 286 34 696.4 0 34 287 58 696.4 0 58 288 60 696.4 0 60 289 148 696.4 0 148 290 41 696.4 0 41 291 59 696.4 0 59 292 44 696.4 0 44 293 25 696.4 0 25 294 30 696.4 0 30 295 17 696.4 0 17 296 36 696.4 0 36 297 68 696.4 0 68 298 52 696.4 0 52 299 87 696.4 0 87 300 33 696.4 0 33 301 31 696.4 0 31 Finished in 5.05 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 87,567 Reads with adapters: 42,566 (48.6%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 42,548 (48.6%) Total basepairs processed: 24,901,128 bp Total written (filtered): 11,519,457 bp (46.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42566 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 18 0.0 3 0 0 18 19 657 0.0 3 0 637 18 0 2 20 41177 0.0 4 40327 816 30 3 1 21 90 0.0 4 17 72 1 22 16 0.0 4 5 1 10 23 32 0.0 4 0 0 0 29 3 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 77 1 0.0 4 1 86 4 0.0 4 0 0 0 0 4 92 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 6 0.0 4 0 0 0 0 6 127 536 0.0 4 0 0 0 0 536 128 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 258 1 0.0 4 0 0 0 0 1 283 3 0.0 4 0 0 0 0 3 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 42,548 Reads with adapters: 6,712 (15.8%) Reads written (passing filters): 42,548 (100.0%) Total basepairs processed: 11,519,457 bp Total written (filtered): 11,423,478 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6712 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 2.6% G: 93.3% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 664.8 0 9 4 3 166.2 0 3 5 4 41.6 0 4 6 1 10.4 0 1 8 1 0.6 0 1 9 3606 0.2 0 111 3495 10 2383 0.0 1 82 2301 11 564 0.0 1 5 559 12 8 0.0 1 0 8 13 5 0.0 1 0 5 149 1 0.0 1 0 1 150 5 0.0 1 0 5 151 1 0.0 1 0 1 152 1 0.0 1 0 1 208 2 0.0 1 0 2 211 1 0.0 1 0 1 229 1 0.0 1 1 257 1 0.0 1 1 260 1 0.0 1 1 269 104 0.0 1 100 4 270 6 0.0 1 6 271 2 0.0 1 2 272 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.