This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.82 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 174,378 (6.1%) Reads that were too short: 40,355 (1.4%) Reads written (passing filters): 134,023 (4.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 35,384,455 bp (4.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 174378 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4843 696.4 0 4843 7 48298 696.4 0 48298 8 45208 696.4 0 45208 9 113 696.4 0 113 10 182 696.4 0 182 11 35 696.4 0 35 12 31 696.4 0 31 13 35 696.4 0 35 14 48 696.4 0 48 15 43 696.4 0 43 16 46 696.4 0 46 17 83 696.4 0 83 18 125 696.4 0 125 19 75 696.4 0 75 20 29 696.4 0 29 21 44 696.4 0 44 22 26 696.4 0 26 23 24 696.4 0 24 24 38 696.4 0 38 25 40 696.4 0 40 26 55 696.4 0 55 27 49 696.4 0 49 28 25 696.4 0 25 29 54 696.4 0 54 30 36 696.4 0 36 31 37 696.4 0 37 32 39 696.4 0 39 33 45 696.4 0 45 34 39 696.4 0 39 35 39 696.4 0 39 36 43 696.4 0 43 37 36 696.4 0 36 38 27 696.4 0 27 39 43 696.4 0 43 40 41 696.4 0 41 41 43 696.4 0 43 42 52 696.4 0 52 43 45 696.4 0 45 44 33 696.4 0 33 45 26 696.4 0 26 46 62 696.4 0 62 47 75 696.4 0 75 48 79 696.4 0 79 49 116 696.4 0 116 50 51 696.4 0 51 51 48 696.4 0 48 52 69 696.4 0 69 53 102 696.4 0 102 54 67 696.4 0 67 55 60 696.4 0 60 56 45 696.4 0 45 57 65 696.4 0 65 58 54 696.4 0 54 59 54 696.4 0 54 60 108 696.4 0 108 61 66 696.4 0 66 62 75 696.4 0 75 63 68 696.4 0 68 64 80 696.4 0 80 65 55 696.4 0 55 66 67 696.4 0 67 67 91 696.4 0 91 68 58 696.4 0 58 69 52 696.4 0 52 70 129 696.4 0 129 71 118 696.4 0 118 72 163 696.4 0 163 73 624 696.4 0 624 74 690 696.4 0 690 75 901 696.4 0 901 76 378 696.4 0 378 77 3361 696.4 0 3361 78 1889 696.4 0 1889 79 1694 696.4 0 1694 80 36 696.4 0 36 81 40 696.4 0 40 82 56 696.4 0 56 83 51 696.4 0 51 84 51 696.4 0 51 85 48 696.4 0 48 86 105 696.4 0 105 87 42 696.4 0 42 88 75 696.4 0 75 89 60 696.4 0 60 90 70 696.4 0 70 91 55 696.4 0 55 92 45 696.4 0 45 93 47 696.4 0 47 94 42 696.4 0 42 95 38 696.4 0 38 96 44 696.4 0 44 97 52 696.4 0 52 98 34 696.4 0 34 99 59 696.4 0 59 100 42 696.4 0 42 101 56 696.4 0 56 102 32 696.4 0 32 103 56 696.4 0 56 104 45 696.4 0 45 105 64 696.4 0 64 106 266 696.4 0 266 107 507 696.4 0 507 108 145 696.4 0 145 109 41 696.4 0 41 110 56 696.4 0 56 111 37 696.4 0 37 112 81 696.4 0 81 113 69 696.4 0 69 114 117 696.4 0 117 115 73 696.4 0 73 116 75 696.4 0 75 117 56 696.4 0 56 118 40 696.4 0 40 119 48 696.4 0 48 120 48 696.4 0 48 121 47 696.4 0 47 122 45 696.4 0 45 123 44 696.4 0 44 124 59 696.4 0 59 125 68 696.4 0 68 126 74 696.4 0 74 127 118 696.4 0 118 128 197 696.4 0 197 129 206 696.4 0 206 130 232 696.4 0 232 131 110 696.4 0 110 132 150 696.4 0 150 133 176 696.4 0 176 134 183 696.4 0 183 135 119 696.4 0 119 136 143 696.4 0 143 137 67 696.4 0 67 138 90 696.4 0 90 139 93 696.4 0 93 140 148 696.4 0 148 141 89 696.4 0 89 142 69 696.4 0 69 143 139 696.4 0 139 144 147 696.4 0 147 145 235 696.4 0 235 146 3940 696.4 0 3940 147 2195 696.4 0 2195 148 2778 696.4 0 2778 149 88 696.4 0 88 150 203 696.4 0 203 151 53 696.4 0 53 152 47 696.4 0 47 153 63 696.4 0 63 154 71 696.4 0 71 155 196 696.4 0 196 156 183 696.4 0 183 157 138 696.4 0 138 158 77 696.4 0 77 159 100 696.4 0 100 160 101 696.4 0 101 161 102 696.4 0 102 162 56 696.4 0 56 163 58 696.4 0 58 164 227 696.4 0 227 165 222 696.4 0 222 166 351 696.4 0 351 167 171 696.4 0 171 168 91 696.4 0 91 169 61 696.4 0 61 170 59 696.4 0 59 171 52 696.4 0 52 172 53 696.4 0 53 173 56 696.4 0 56 174 66 696.4 0 66 175 41 696.4 0 41 176 43 696.4 0 43 177 59 696.4 0 59 178 71 696.4 0 71 179 75 696.4 0 75 180 78 696.4 0 78 181 97 696.4 0 97 182 69 696.4 0 69 183 70 696.4 0 70 184 243 696.4 0 243 185 285 696.4 0 285 186 361 696.4 0 361 187 117 696.4 0 117 188 74 696.4 0 74 189 53 696.4 0 53 190 56 696.4 0 56 191 61 696.4 0 61 192 84 696.4 0 84 193 81 696.4 0 81 194 100 696.4 0 100 195 631 696.4 0 631 196 451 696.4 0 451 197 556 696.4 0 556 198 252 696.4 0 252 199 133 696.4 0 133 200 100 696.4 0 100 201 85 696.4 0 85 202 61 696.4 0 61 203 51 696.4 0 51 204 91 696.4 0 91 205 106 696.4 0 106 206 92 696.4 0 92 207 66 696.4 0 66 208 68 696.4 0 68 209 103 696.4 0 103 210 70 696.4 0 70 211 80 696.4 0 80 212 80 696.4 0 80 213 84 696.4 0 84 214 89 696.4 0 89 215 209 696.4 0 209 216 137 696.4 0 137 217 145 696.4 0 145 218 87 696.4 0 87 219 162 696.4 0 162 220 80 696.4 0 80 221 98 696.4 0 98 222 77 696.4 0 77 223 65 696.4 0 65 224 90 696.4 0 90 225 79 696.4 0 79 226 97 696.4 0 97 227 96 696.4 0 96 228 83 696.4 0 83 229 81 696.4 0 81 230 87 696.4 0 87 231 128 696.4 0 128 232 151 696.4 0 151 233 229 696.4 0 229 234 376 696.4 0 376 235 761 696.4 0 761 236 6962 696.4 0 6962 237 7962 696.4 0 7962 238 4391 696.4 0 4391 239 161 696.4 0 161 240 92 696.4 0 92 241 134 696.4 0 134 242 82 696.4 0 82 243 73 696.4 0 73 244 94 696.4 0 94 245 142 696.4 0 142 246 220 696.4 0 220 247 336 696.4 0 336 248 499 696.4 0 499 249 294 696.4 0 294 250 156 696.4 0 156 251 254 696.4 0 254 252 382 696.4 0 382 253 408 696.4 0 408 254 293 696.4 0 293 255 319 696.4 0 319 256 166 696.4 0 166 257 91 696.4 0 91 258 70 696.4 0 70 259 85 696.4 0 85 260 66 696.4 0 66 261 58 696.4 0 58 262 68 696.4 0 68 263 88 696.4 0 88 264 84 696.4 0 84 265 101 696.4 0 101 266 99 696.4 0 99 267 80 696.4 0 80 268 98 696.4 0 98 269 87 696.4 0 87 270 118 696.4 0 118 271 100 696.4 0 100 272 99 696.4 0 99 273 119 696.4 0 119 274 111 696.4 0 111 275 97 696.4 0 97 276 135 696.4 0 135 277 187 696.4 0 187 278 277 696.4 0 277 279 200 696.4 0 200 280 382 696.4 0 382 281 518 696.4 0 518 282 647 696.4 0 647 283 694 696.4 0 694 284 610 696.4 0 610 285 434 696.4 0 434 286 412 696.4 0 412 287 275 696.4 0 275 288 166 696.4 0 166 289 153 696.4 0 153 290 102 696.4 0 102 291 128 696.4 0 128 292 165 696.4 0 165 293 286 696.4 0 286 294 305 696.4 0 305 295 490 696.4 0 490 296 1249 696.4 0 1249 297 1318 696.4 0 1318 298 741 696.4 0 741 299 376 696.4 0 376 300 239 696.4 0 239 301 368 696.4 0 368 Finished in 6.88 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 134,023 Reads with adapters: 50,663 (37.8%) Reads that were too short: 170 (0.1%) Reads written (passing filters): 50,493 (37.7%) Total basepairs processed: 35,384,455 bp Total written (filtered): 13,887,550 bp (39.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50663 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 24 0.0 3 0 0 10 14 16 362 0.0 3 0 296 45 21 17 48990 0.0 3 45616 2756 490 128 18 136 0.0 3 14 102 13 7 19 84 0.0 3 0 0 45 39 20 258 0.0 3 1 0 0 257 22 2 0.0 3 0 0 1 1 23 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 1 0 0 1 32 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 0 4 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 39 36 0.0 3 1 0 0 35 42 1 0.0 3 0 0 0 1 43 11 0.0 3 0 0 0 11 44 3 0.0 3 0 0 0 3 45 184 0.0 3 0 0 1 183 46 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 21 0.0 3 0 0 0 21 49 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 52 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 1 57 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 4 0.0 3 0 0 0 4 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 4 0.0 3 0 0 0 4 89 4 0.0 3 0 0 0 4 90 1 0.0 3 0 0 0 1 91 5 0.0 3 0 0 0 5 92 2 0.0 3 0 0 0 2 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 13 0.0 3 0 0 0 13 99 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 105 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 1 1 114 199 0.0 3 0 0 1 198 115 55 0.0 3 0 0 2 53 116 1 0.0 3 0 0 0 1 117 16 0.0 3 0 0 1 15 118 18 0.0 3 0 1 0 17 119 3 0.0 3 0 0 0 3 120 5 0.0 3 0 0 0 5 121 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 123 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 5 0.0 3 0 0 0 5 146 11 0.0 3 0 0 0 11 150 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 2 0.0 3 0 0 0 2 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 165 5 0.0 3 0 0 0 5 166 2 0.0 3 0 0 0 2 167 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 177 2 0.0 3 0 0 0 2 178 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 9 0.0 3 0 0 1 8 195 2 0.0 3 0 0 0 2 197 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 1 205 2 0.0 3 0 0 0 2 206 5 0.0 3 0 0 0 5 210 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 213 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 215 5 0.0 3 0 0 0 5 216 2 0.0 3 0 0 0 2 217 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 1 1 219 2 0.0 3 0 0 1 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 4 0.0 3 0 0 0 4 281 9 0.0 3 0 0 1 8 283 1 0.0 3 0 0 1 284 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 50,493 Reads with adapters: 365 (0.7%) Reads written (passing filters): 50,493 (100.0%) Total basepairs processed: 13,887,550 bp Total written (filtered): 13,886,382 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 365 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.8% C: 48.5% G: 24.7% T: 9.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 329 789.0 0 329 4 15 197.2 0 15 5 15 49.3 0 15 6 4 12.3 0 4 10 1 0.0 1 0 1 12 1 0.0 1 0 1