This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 74.80 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 162,725 (5.7%) Reads that were too short: 33,470 (1.2%) Reads written (passing filters): 129,255 (4.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 34,979,129 bp (4.1%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 162725 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4013 696.4 0 4013 7 47359 696.4 0 47359 8 50798 696.4 0 50798 9 88 696.4 0 88 10 127 696.4 0 127 11 28 696.4 0 28 12 33 696.4 0 33 13 38 696.4 0 38 14 47 696.4 0 47 15 36 696.4 0 36 16 36 696.4 0 36 17 33 696.4 0 33 18 38 696.4 0 38 19 36 696.4 0 36 20 34 696.4 0 34 21 42 696.4 0 42 22 33 696.4 0 33 23 23 696.4 0 23 24 39 696.4 0 39 25 37 696.4 0 37 26 41 696.4 0 41 27 33 696.4 0 33 28 46 696.4 0 46 29 50 696.4 0 50 30 47 696.4 0 47 31 35 696.4 0 35 32 34 696.4 0 34 33 44 696.4 0 44 34 32 696.4 0 32 35 43 696.4 0 43 36 41 696.4 0 41 37 37 696.4 0 37 38 35 696.4 0 35 39 39 696.4 0 39 40 54 696.4 0 54 41 32 696.4 0 32 42 49 696.4 0 49 43 47 696.4 0 47 44 37 696.4 0 37 45 47 696.4 0 47 46 74 696.4 0 74 47 53 696.4 0 53 48 43 696.4 0 43 49 54 696.4 0 54 50 37 696.4 0 37 51 36 696.4 0 36 52 70 696.4 0 70 53 70 696.4 0 70 54 55 696.4 0 55 55 55 696.4 0 55 56 53 696.4 0 53 57 54 696.4 0 54 58 47 696.4 0 47 59 48 696.4 0 48 60 83 696.4 0 83 61 77 696.4 0 77 62 1867 696.4 0 1867 63 96 696.4 0 96 64 61 696.4 0 61 65 52 696.4 0 52 66 45 696.4 0 45 67 62 696.4 0 62 68 52 696.4 0 52 69 47 696.4 0 47 70 54 696.4 0 54 71 64 696.4 0 64 72 81 696.4 0 81 73 386 696.4 0 386 74 486 696.4 0 486 75 544 696.4 0 544 76 119 696.4 0 119 77 1302 696.4 0 1302 78 733 696.4 0 733 79 708 696.4 0 708 80 56 696.4 0 56 81 47 696.4 0 47 82 65 696.4 0 65 83 37 696.4 0 37 84 29 696.4 0 29 85 53 696.4 0 53 86 35 696.4 0 35 87 38 696.4 0 38 88 57 696.4 0 57 89 63 696.4 0 63 90 43 696.4 0 43 91 45 696.4 0 45 92 57 696.4 0 57 93 42 696.4 0 42 94 41 696.4 0 41 95 42 696.4 0 42 96 50 696.4 0 50 97 55 696.4 0 55 98 41 696.4 0 41 99 52 696.4 0 52 100 39 696.4 0 39 101 52 696.4 0 52 102 41 696.4 0 41 103 57 696.4 0 57 104 67 696.4 0 67 105 75 696.4 0 75 106 330 696.4 0 330 107 663 696.4 0 663 108 142 696.4 0 142 109 59 696.4 0 59 110 45 696.4 0 45 111 47 696.4 0 47 112 69 696.4 0 69 113 53 696.4 0 53 114 108 696.4 0 108 115 60 696.4 0 60 116 44 696.4 0 44 117 60 696.4 0 60 118 45 696.4 0 45 119 49 696.4 0 49 120 63 696.4 0 63 121 60 696.4 0 60 122 54 696.4 0 54 123 59 696.4 0 59 124 52 696.4 0 52 125 61 696.4 0 61 126 72 696.4 0 72 127 111 696.4 0 111 128 142 696.4 0 142 129 154 696.4 0 154 130 205 696.4 0 205 131 93 696.4 0 93 132 158 696.4 0 158 133 129 696.4 0 129 134 143 696.4 0 143 135 103 696.4 0 103 136 119 696.4 0 119 137 58 696.4 0 58 138 69 696.4 0 69 139 108 696.4 0 108 140 111 696.4 0 111 141 59 696.4 0 59 142 61 696.4 0 61 143 113 696.4 0 113 144 102 696.4 0 102 145 190 696.4 0 190 146 3334 696.4 0 3334 147 1817 696.4 0 1817 148 2357 696.4 0 2357 149 90 696.4 0 90 150 223 696.4 0 223 151 51 696.4 0 51 152 31 696.4 0 31 153 43 696.4 0 43 154 62 696.4 0 62 155 167 696.4 0 167 156 174 696.4 0 174 157 105 696.4 0 105 158 45 696.4 0 45 159 60 696.4 0 60 160 53 696.4 0 53 161 78 696.4 0 78 162 47 696.4 0 47 163 46 696.4 0 46 164 117 696.4 0 117 165 120 696.4 0 120 166 169 696.4 0 169 167 94 696.4 0 94 168 32 696.4 0 32 169 46 696.4 0 46 170 41 696.4 0 41 171 42 696.4 0 42 172 39 696.4 0 39 173 49 696.4 0 49 174 50 696.4 0 50 175 36 696.4 0 36 176 27 696.4 0 27 177 34 696.4 0 34 178 53 696.4 0 53 179 57 696.4 0 57 180 35 696.4 0 35 181 46 696.4 0 46 182 49 696.4 0 49 183 38 696.4 0 38 184 118 696.4 0 118 185 160 696.4 0 160 186 153 696.4 0 153 187 83 696.4 0 83 188 49 696.4 0 49 189 43 696.4 0 43 190 27 696.4 0 27 191 51 696.4 0 51 192 52 696.4 0 52 193 51 696.4 0 51 194 71 696.4 0 71 195 445 696.4 0 445 196 295 696.4 0 295 197 286 696.4 0 286 198 114 696.4 0 114 199 88 696.4 0 88 200 49 696.4 0 49 201 52 696.4 0 52 202 46 696.4 0 46 203 50 696.4 0 50 204 68 696.4 0 68 205 80 696.4 0 80 206 70 696.4 0 70 207 34 696.4 0 34 208 33 696.4 0 33 209 85 696.4 0 85 210 39 696.4 0 39 211 32 696.4 0 32 212 47 696.4 0 47 213 46 696.4 0 46 214 50 696.4 0 50 215 129 696.4 0 129 216 111 696.4 0 111 217 99 696.4 0 99 218 66 696.4 0 66 219 106 696.4 0 106 220 80 696.4 0 80 221 57 696.4 0 57 222 42 696.4 0 42 223 53 696.4 0 53 224 60 696.4 0 60 225 46 696.4 0 46 226 49 696.4 0 49 227 37 696.4 0 37 228 52 696.4 0 52 229 55 696.4 0 55 230 71 696.4 0 71 231 120 696.4 0 120 232 122 696.4 0 122 233 214 696.4 0 214 234 321 696.4 0 321 235 569 696.4 0 569 236 7869 696.4 0 7869 237 9892 696.4 0 9892 238 5232 696.4 0 5232 239 79 696.4 0 79 240 44 696.4 0 44 241 56 696.4 0 56 242 38 696.4 0 38 243 33 696.4 0 33 244 35 696.4 0 35 245 65 696.4 0 65 246 88 696.4 0 88 247 142 696.4 0 142 248 176 696.4 0 176 249 90 696.4 0 90 250 56 696.4 0 56 251 78 696.4 0 78 252 105 696.4 0 105 253 107 696.4 0 107 254 72 696.4 0 72 255 82 696.4 0 82 256 54 696.4 0 54 257 36 696.4 0 36 258 44 696.4 0 44 259 40 696.4 0 40 260 29 696.4 0 29 261 40 696.4 0 40 262 30 696.4 0 30 263 47 696.4 0 47 264 44 696.4 0 44 265 54 696.4 0 54 266 45 696.4 0 45 267 48 696.4 0 48 268 54 696.4 0 54 269 56 696.4 0 56 270 36 696.4 0 36 271 68 696.4 0 68 272 47 696.4 0 47 273 52 696.4 0 52 274 43 696.4 0 43 275 41 696.4 0 41 276 73 696.4 0 73 277 103 696.4 0 103 278 164 696.4 0 164 279 98 696.4 0 98 280 186 696.4 0 186 281 372 696.4 0 372 282 401 696.4 0 401 283 452 696.4 0 452 284 228 696.4 0 228 285 176 696.4 0 176 286 135 696.4 0 135 287 74 696.4 0 74 288 66 696.4 0 66 289 83 696.4 0 83 290 77 696.4 0 77 291 65 696.4 0 65 292 83 696.4 0 83 293 161 696.4 0 161 294 134 696.4 0 134 295 231 696.4 0 231 296 457 696.4 0 457 297 462 696.4 0 462 298 276 696.4 0 276 299 164 696.4 0 164 300 116 696.4 0 116 301 247 696.4 0 247 Finished in 7.20 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 129,255 Reads with adapters: 53,863 (41.7%) Reads that were too short: 40 (0.0%) Reads written (passing filters): 53,823 (41.6%) Total basepairs processed: 34,979,129 bp Total written (filtered): 14,676,988 bp (42.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53863 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 35 0.0 3 0 0 33 1 1 19 610 0.0 3 0 595 14 1 20 51503 0.0 4 50552 918 31 1 1 21 157 0.0 4 31 119 6 0 1 22 1277 0.0 4 1241 28 8 23 59 0.0 4 1 3 0 48 7 24 2 0.0 4 0 0 0 0 2 25 2 0.0 4 0 1 0 0 1 26 1 0.0 4 0 0 0 0 1 57 29 0.0 4 0 0 0 0 29 61 10 0.0 4 0 0 0 0 10 74 2 0.0 4 0 0 0 0 2 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 124 0.0 4 0 0 0 0 124 135 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 1 153 2 0.0 4 0 0 0 0 2 162 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 20 0.0 4 0 0 0 0 20 176 1 0.0 4 1 207 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 1 3 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 1 236 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.87 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 53,823 Reads with adapters: 3,256 (6.0%) Reads written (passing filters): 53,823 (100.0%) Total basepairs processed: 14,676,988 bp Total written (filtered): 14,641,747 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3256 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 3.8% G: 91.5% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 50 841.0 0 50 4 13 210.2 0 13 5 3 52.6 0 3 6 6 13.1 0 6 8 1 0.8 0 1 9 54 0.2 0 1 53 10 980 0.1 1 30 950 11 2130 0.0 1 15 2115 12 9 0.0 1 0 9 13 3 0.0 1 0 3 37 1 0.0 1 0 1 112 1 0.0 1 1 151 1 0.0 1 0 1 190 1 0.0 1 0 1 209 3 0.0 1 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.