This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 73.69 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 77,054 (2.7%) Reads that were too short: 48,702 (1.7%) Reads written (passing filters): 28,352 (1.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 5,977,307 bp (0.7%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 77054 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 170 696.4 0 170 7 4989 696.4 0 4989 8 85 696.4 0 85 9 3892 696.4 0 3892 10 21 696.4 0 21 11 10 696.4 0 10 12 25 696.4 0 25 13 16 696.4 0 16 14 11 696.4 0 11 15 5 696.4 0 5 16 11 696.4 0 11 17 16 696.4 0 16 18 15 696.4 0 15 19 11 696.4 0 11 20 9 696.4 0 9 21 9 696.4 0 9 22 12 696.4 0 12 23 11 696.4 0 11 24 23 696.4 0 23 25 13 696.4 0 13 26 9 696.4 0 9 27 6 696.4 0 6 28 5 696.4 0 5 29 8 696.4 0 8 30 10 696.4 0 10 31 10 696.4 0 10 32 19 696.4 0 19 33 15 696.4 0 15 34 10 696.4 0 10 35 16 696.4 0 16 36 24 696.4 0 24 37 13 696.4 0 13 38 15 696.4 0 15 39 17 696.4 0 17 40 9 696.4 0 9 41 22 696.4 0 22 42 15 696.4 0 15 43 96 696.4 0 96 44 13 696.4 0 13 45 6 696.4 0 6 46 12 696.4 0 12 47 8 696.4 0 8 48 16 696.4 0 16 49 10 696.4 0 10 50 10 696.4 0 10 51 12 696.4 0 12 52 7 696.4 0 7 53 12 696.4 0 12 54 18 696.4 0 18 55 1219 696.4 0 1219 56 52 696.4 0 52 57 13 696.4 0 13 58 12 696.4 0 12 59 15 696.4 0 15 60 9 696.4 0 9 61 17 696.4 0 17 62 11 696.4 0 11 63 8 696.4 0 8 64 11 696.4 0 11 65 15 696.4 0 15 66 20 696.4 0 20 67 19 696.4 0 19 68 18 696.4 0 18 69 23 696.4 0 23 70 44 696.4 0 44 71 47 696.4 0 47 72 44 696.4 0 44 73 20 696.4 0 20 74 28 696.4 0 28 75 52 696.4 0 52 76 103 696.4 0 103 77 79 696.4 0 79 78 94 696.4 0 94 79 50 696.4 0 50 80 135 696.4 0 135 81 379 696.4 0 379 82 223 696.4 0 223 83 131 696.4 0 131 84 36 696.4 0 36 85 37 696.4 0 37 86 32 696.4 0 32 87 35 696.4 0 35 88 19 696.4 0 19 89 36 696.4 0 36 90 36 696.4 0 36 91 22 696.4 0 22 92 30 696.4 0 30 93 27 696.4 0 27 94 34 696.4 0 34 95 393 696.4 0 393 96 58 696.4 0 58 97 30 696.4 0 30 98 61 696.4 0 61 99 31 696.4 0 31 100 44 696.4 0 44 101 229 696.4 0 229 102 294 696.4 0 294 103 345 696.4 0 345 104 151 696.4 0 151 105 69 696.4 0 69 106 72 696.4 0 72 107 100 696.4 0 100 108 102 696.4 0 102 109 131 696.4 0 131 110 157 696.4 0 157 111 171 696.4 0 171 112 144 696.4 0 144 113 137 696.4 0 137 114 103 696.4 0 103 115 118 696.4 0 118 116 97 696.4 0 97 117 102 696.4 0 102 118 147 696.4 0 147 119 240 696.4 0 240 120 98 696.4 0 98 121 62 696.4 0 62 122 118 696.4 0 118 123 141 696.4 0 141 124 173 696.4 0 173 125 139 696.4 0 139 126 116 696.4 0 116 127 133 696.4 0 133 128 70 696.4 0 70 129 90 696.4 0 90 130 101 696.4 0 101 131 139 696.4 0 139 132 244 696.4 0 244 133 553 696.4 0 553 134 250 696.4 0 250 135 300 696.4 0 300 136 110 696.4 0 110 137 113 696.4 0 113 138 202 696.4 0 202 139 209 696.4 0 209 140 218 696.4 0 218 141 150 696.4 0 150 142 94 696.4 0 94 143 102 696.4 0 102 144 163 696.4 0 163 145 150 696.4 0 150 146 300 696.4 0 300 147 221 696.4 0 221 148 255 696.4 0 255 149 119 696.4 0 119 150 76 696.4 0 76 151 121 696.4 0 121 152 107 696.4 0 107 153 159 696.4 0 159 154 158 696.4 0 158 155 127 696.4 0 127 156 180 696.4 0 180 157 183 696.4 0 183 158 238 696.4 0 238 159 140 696.4 0 140 160 151 696.4 0 151 161 142 696.4 0 142 162 110 696.4 0 110 163 96 696.4 0 96 164 119 696.4 0 119 165 147 696.4 0 147 166 130 696.4 0 130 167 106 696.4 0 106 168 94 696.4 0 94 169 89 696.4 0 89 170 111 696.4 0 111 171 121 696.4 0 121 172 155 696.4 0 155 173 134 696.4 0 134 174 106 696.4 0 106 175 80 696.4 0 80 176 81 696.4 0 81 177 86 696.4 0 86 178 85 696.4 0 85 179 120 696.4 0 120 180 259 696.4 0 259 181 256 696.4 0 256 182 218 696.4 0 218 183 90 696.4 0 90 184 124 696.4 0 124 185 162 696.4 0 162 186 101 696.4 0 101 187 102 696.4 0 102 188 116 696.4 0 116 189 96 696.4 0 96 190 134 696.4 0 134 191 108 696.4 0 108 192 98 696.4 0 98 193 105 696.4 0 105 194 134 696.4 0 134 195 94 696.4 0 94 196 93 696.4 0 93 197 99 696.4 0 99 198 130 696.4 0 130 199 142 696.4 0 142 200 130 696.4 0 130 201 118 696.4 0 118 202 80 696.4 0 80 203 142 696.4 0 142 204 124 696.4 0 124 205 165 696.4 0 165 206 245 696.4 0 245 207 234 696.4 0 234 208 303 696.4 0 303 209 443 696.4 0 443 210 1250 696.4 0 1250 211 10456 696.4 0 10456 212 11624 696.4 0 11624 213 4691 696.4 0 4691 214 1399 696.4 0 1399 215 121 696.4 0 121 216 96 696.4 0 96 217 91 696.4 0 91 218 162 696.4 0 162 219 153 696.4 0 153 220 126 696.4 0 126 221 97 696.4 0 97 222 85 696.4 0 85 223 138 696.4 0 138 224 234 696.4 0 234 225 176 696.4 0 176 226 190 696.4 0 190 227 121 696.4 0 121 228 131 696.4 0 131 229 126 696.4 0 126 230 111 696.4 0 111 231 98 696.4 0 98 232 90 696.4 0 90 233 91 696.4 0 91 234 129 696.4 0 129 235 103 696.4 0 103 236 102 696.4 0 102 237 132 696.4 0 132 238 132 696.4 0 132 239 133 696.4 0 133 240 137 696.4 0 137 241 118 696.4 0 118 242 99 696.4 0 99 243 120 696.4 0 120 244 275 696.4 0 275 245 216 696.4 0 216 246 737 696.4 0 737 247 109 696.4 0 109 248 70 696.4 0 70 249 80 696.4 0 80 250 96 696.4 0 96 251 104 696.4 0 104 252 124 696.4 0 124 253 109 696.4 0 109 254 118 696.4 0 118 255 146 696.4 0 146 256 196 696.4 0 196 257 173 696.4 0 173 258 185 696.4 0 185 259 135 696.4 0 135 260 218 696.4 0 218 261 135 696.4 0 135 262 119 696.4 0 119 263 90 696.4 0 90 264 97 696.4 0 97 265 132 696.4 0 132 266 192 696.4 0 192 267 271 696.4 0 271 268 205 696.4 0 205 269 120 696.4 0 120 270 292 696.4 0 292 271 249 696.4 0 249 272 519 696.4 0 519 273 237 696.4 0 237 274 390 696.4 0 390 275 270 696.4 0 270 276 196 696.4 0 196 277 253 696.4 0 253 278 187 696.4 0 187 279 262 696.4 0 262 280 179 696.4 0 179 281 135 696.4 0 135 282 129 696.4 0 129 283 120 696.4 0 120 284 99 696.4 0 99 285 119 696.4 0 119 286 137 696.4 0 137 287 205 696.4 0 205 288 194 696.4 0 194 289 156 696.4 0 156 290 138 696.4 0 138 291 201 696.4 0 201 292 864 696.4 0 864 293 924 696.4 0 924 294 1020 696.4 0 1020 295 493 696.4 0 493 296 182 696.4 0 182 297 108 696.4 0 108 298 152 696.4 0 152 299 161 696.4 0 161 300 256 696.4 0 256 301 625 696.4 0 625 Finished in 1.15 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 28,352 Reads with adapters: 4,123 (14.5%) Reads that were too short: 56 (0.2%) Reads written (passing filters): 4,067 (14.3%) Total basepairs processed: 5,977,307 bp Total written (filtered): 1,110,222 bp (18.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4123 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 6 0.0 3 0 0 4 2 16 64 0.0 3 0 56 6 2 17 3890 0.0 3 3638 216 31 5 18 21 0.0 3 1 10 3 7 19 21 0.0 3 11 5 4 1 20 8 0.0 3 0 1 0 7 21 6 0.0 3 0 0 0 6 29 1 0.0 3 0 0 0 1 33 8 0.0 3 0 0 0 8 34 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 1 0 1 38 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 59 8 0.0 3 0 0 0 8 60 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 70 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 75 1 0.0 3 0 0 0 1 76 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 3 0.0 3 0 0 0 3 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 1 3 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 1 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 179 2 0.0 3 0 0 0 2 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 4,067 Reads with adapters: 103 (2.5%) Reads written (passing filters): 4,067 (100.0%) Total basepairs processed: 1,110,222 bp Total written (filtered): 1,109,910 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 103 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 66.0% C: 20.4% G: 10.7% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 100 63.5 0 100 4 3 15.9 0 3