This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 73.37 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 77,205 (2.7%) Reads that were too short: 48,153 (1.7%) Reads written (passing filters): 29,052 (1.0%) Total basepairs processed: 858,556,748 bp Total written (filtered): 6,134,734 bp (0.7%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 77205 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 121 696.4 0 121 7 4790 696.4 0 4790 8 62 696.4 0 62 9 4439 696.4 0 4439 10 14 696.4 0 14 11 21 696.4 0 21 12 21 696.4 0 21 13 14 696.4 0 14 14 12 696.4 0 12 15 20 696.4 0 20 16 14 696.4 0 14 17 24 696.4 0 24 18 15 696.4 0 15 19 14 696.4 0 14 20 17 696.4 0 17 21 19 696.4 0 19 22 13 696.4 0 13 23 18 696.4 0 18 24 19 696.4 0 19 25 10 696.4 0 10 26 16 696.4 0 16 27 17 696.4 0 17 28 9 696.4 0 9 29 12 696.4 0 12 30 20 696.4 0 20 31 6 696.4 0 6 32 20 696.4 0 20 33 21 696.4 0 21 34 101 696.4 0 101 35 19 696.4 0 19 36 21 696.4 0 21 37 17 696.4 0 17 38 18 696.4 0 18 39 24 696.4 0 24 40 12 696.4 0 12 41 33 696.4 0 33 42 803 696.4 0 803 43 169 696.4 0 169 44 13 696.4 0 13 45 13 696.4 0 13 46 16 696.4 0 16 47 17 696.4 0 17 48 16 696.4 0 16 49 10 696.4 0 10 50 11 696.4 0 11 51 12 696.4 0 12 52 16 696.4 0 16 53 10 696.4 0 10 54 15 696.4 0 15 55 18 696.4 0 18 56 17 696.4 0 17 57 15 696.4 0 15 58 12 696.4 0 12 59 18 696.4 0 18 60 15 696.4 0 15 61 15 696.4 0 15 62 19 696.4 0 19 63 14 696.4 0 14 64 22 696.4 0 22 65 25 696.4 0 25 66 21 696.4 0 21 67 23 696.4 0 23 68 5 696.4 0 5 69 25 696.4 0 25 70 24 696.4 0 24 71 25 696.4 0 25 72 24 696.4 0 24 73 12 696.4 0 12 74 17 696.4 0 17 75 21 696.4 0 21 76 16 696.4 0 16 77 24 696.4 0 24 78 72 696.4 0 72 79 71 696.4 0 71 80 93 696.4 0 93 81 471 696.4 0 471 82 289 696.4 0 289 83 258 696.4 0 258 84 104 696.4 0 104 85 158 696.4 0 158 86 153 696.4 0 153 87 129 696.4 0 129 88 90 696.4 0 90 89 74 696.4 0 74 90 77 696.4 0 77 91 73 696.4 0 73 92 78 696.4 0 78 93 77 696.4 0 77 94 69 696.4 0 69 95 114 696.4 0 114 96 68 696.4 0 68 97 80 696.4 0 80 98 84 696.4 0 84 99 43 696.4 0 43 100 45 696.4 0 45 101 104 696.4 0 104 102 199 696.4 0 199 103 224 696.4 0 224 104 154 696.4 0 154 105 84 696.4 0 84 106 66 696.4 0 66 107 114 696.4 0 114 108 84 696.4 0 84 109 119 696.4 0 119 110 156 696.4 0 156 111 149 696.4 0 149 112 140 696.4 0 140 113 124 696.4 0 124 114 115 696.4 0 115 115 125 696.4 0 125 116 78 696.4 0 78 117 128 696.4 0 128 118 129 696.4 0 129 119 229 696.4 0 229 120 127 696.4 0 127 121 77 696.4 0 77 122 100 696.4 0 100 123 117 696.4 0 117 124 187 696.4 0 187 125 177 696.4 0 177 126 128 696.4 0 128 127 157 696.4 0 157 128 93 696.4 0 93 129 108 696.4 0 108 130 89 696.4 0 89 131 128 696.4 0 128 132 200 696.4 0 200 133 396 696.4 0 396 134 258 696.4 0 258 135 313 696.4 0 313 136 118 696.4 0 118 137 121 696.4 0 121 138 219 696.4 0 219 139 223 696.4 0 223 140 227 696.4 0 227 141 153 696.4 0 153 142 91 696.4 0 91 143 104 696.4 0 104 144 143 696.4 0 143 145 142 696.4 0 142 146 341 696.4 0 341 147 232 696.4 0 232 148 266 696.4 0 266 149 136 696.4 0 136 150 86 696.4 0 86 151 159 696.4 0 159 152 140 696.4 0 140 153 159 696.4 0 159 154 134 696.4 0 134 155 143 696.4 0 143 156 209 696.4 0 209 157 160 696.4 0 160 158 246 696.4 0 246 159 139 696.4 0 139 160 150 696.4 0 150 161 124 696.4 0 124 162 119 696.4 0 119 163 116 696.4 0 116 164 163 696.4 0 163 165 151 696.4 0 151 166 149 696.4 0 149 167 107 696.4 0 107 168 114 696.4 0 114 169 112 696.4 0 112 170 126 696.4 0 126 171 119 696.4 0 119 172 149 696.4 0 149 173 132 696.4 0 132 174 124 696.4 0 124 175 90 696.4 0 90 176 83 696.4 0 83 177 102 696.4 0 102 178 78 696.4 0 78 179 149 696.4 0 149 180 236 696.4 0 236 181 185 696.4 0 185 182 166 696.4 0 166 183 111 696.4 0 111 184 140 696.4 0 140 185 164 696.4 0 164 186 144 696.4 0 144 187 122 696.4 0 122 188 101 696.4 0 101 189 109 696.4 0 109 190 128 696.4 0 128 191 130 696.4 0 130 192 131 696.4 0 131 193 105 696.4 0 105 194 144 696.4 0 144 195 88 696.4 0 88 196 106 696.4 0 106 197 80 696.4 0 80 198 139 696.4 0 139 199 174 696.4 0 174 200 140 696.4 0 140 201 124 696.4 0 124 202 94 696.4 0 94 203 141 696.4 0 141 204 127 696.4 0 127 205 190 696.4 0 190 206 226 696.4 0 226 207 206 696.4 0 206 208 352 696.4 0 352 209 439 696.4 0 439 210 905 696.4 0 905 211 10777 696.4 0 10777 212 12389 696.4 0 12389 213 5175 696.4 0 5175 214 1579 696.4 0 1579 215 169 696.4 0 169 216 83 696.4 0 83 217 106 696.4 0 106 218 136 696.4 0 136 219 171 696.4 0 171 220 161 696.4 0 161 221 111 696.4 0 111 222 80 696.4 0 80 223 127 696.4 0 127 224 258 696.4 0 258 225 173 696.4 0 173 226 193 696.4 0 193 227 111 696.4 0 111 228 114 696.4 0 114 229 136 696.4 0 136 230 123 696.4 0 123 231 115 696.4 0 115 232 111 696.4 0 111 233 105 696.4 0 105 234 140 696.4 0 140 235 145 696.4 0 145 236 150 696.4 0 150 237 124 696.4 0 124 238 130 696.4 0 130 239 144 696.4 0 144 240 131 696.4 0 131 241 124 696.4 0 124 242 124 696.4 0 124 243 142 696.4 0 142 244 295 696.4 0 295 245 202 696.4 0 202 246 714 696.4 0 714 247 146 696.4 0 146 248 92 696.4 0 92 249 84 696.4 0 84 250 81 696.4 0 81 251 107 696.4 0 107 252 110 696.4 0 110 253 107 696.4 0 107 254 117 696.4 0 117 255 103 696.4 0 103 256 165 696.4 0 165 257 139 696.4 0 139 258 139 696.4 0 139 259 129 696.4 0 129 260 164 696.4 0 164 261 116 696.4 0 116 262 141 696.4 0 141 263 116 696.4 0 116 264 123 696.4 0 123 265 114 696.4 0 114 266 154 696.4 0 154 267 177 696.4 0 177 268 164 696.4 0 164 269 103 696.4 0 103 270 211 696.4 0 211 271 185 696.4 0 185 272 345 696.4 0 345 273 213 696.4 0 213 274 261 696.4 0 261 275 233 696.4 0 233 276 152 696.4 0 152 277 208 696.4 0 208 278 164 696.4 0 164 279 209 696.4 0 209 280 137 696.4 0 137 281 113 696.4 0 113 282 128 696.4 0 128 283 96 696.4 0 96 284 130 696.4 0 130 285 139 696.4 0 139 286 128 696.4 0 128 287 249 696.4 0 249 288 158 696.4 0 158 289 143 696.4 0 143 290 115 696.4 0 115 291 156 696.4 0 156 292 681 696.4 0 681 293 601 696.4 0 601 294 679 696.4 0 679 295 341 696.4 0 341 296 121 696.4 0 121 297 120 696.4 0 120 298 119 696.4 0 119 299 165 696.4 0 165 300 213 696.4 0 213 301 601 696.4 0 601 Finished in 1.42 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 29,052 Reads with adapters: 4,837 (16.6%) Reads that were too short: 14 (0.0%) Reads written (passing filters): 4,823 (16.6%) Total basepairs processed: 6,134,734 bp Total written (filtered): 1,320,803 bp (21.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4837 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 0 0 1 19 32 0.0 3 0 30 0 0 2 20 4770 0.0 4 4660 102 8 21 10 0.0 4 1 8 0 0 1 22 1 0.0 4 1 23 4 0.0 4 0 0 0 3 1 39 2 0.0 4 0 0 0 0 2 49 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 1 97 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 4,823 Reads with adapters: 41 (0.9%) Reads written (passing filters): 4,823 (100.0%) Total basepairs processed: 1,320,803 bp Total written (filtered): 1,319,763 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 41 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 12.2% C: 17.1% G: 63.4% T: 7.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 7 75.4 0 7 4 1 18.8 0 1 9 1 0.0 0 0 1 10 1 0.0 1 0 1 11 16 0.0 1 0 16 12 11 0.0 1 1 10 13 1 0.0 1 0 1 225 3 0.0 1 3