This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.99 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 196,090 (6.9%) Reads that were too short: 119,460 (4.2%) Reads written (passing filters): 76,630 (2.7%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,493,206 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 196090 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1075 696.4 0 1075 7 754 696.4 0 754 8 20465 696.4 0 20465 9 18252 696.4 0 18252 10 146 696.4 0 146 11 118 696.4 0 118 12 48 696.4 0 48 13 73 696.4 0 73 14 67 696.4 0 67 15 44 696.4 0 44 16 47 696.4 0 47 17 81 696.4 0 81 18 56 696.4 0 56 19 41 696.4 0 41 20 52 696.4 0 52 21 73 696.4 0 73 22 27 696.4 0 27 23 30 696.4 0 30 24 46 696.4 0 46 25 29 696.4 0 29 26 61 696.4 0 61 27 44 696.4 0 44 28 42 696.4 0 42 29 38 696.4 0 38 30 54 696.4 0 54 31 54 696.4 0 54 32 43 696.4 0 43 33 40 696.4 0 40 34 44 696.4 0 44 35 36 696.4 0 36 36 27 696.4 0 27 37 47 696.4 0 47 38 87 696.4 0 87 39 28 696.4 0 28 40 40 696.4 0 40 41 48 696.4 0 48 42 43 696.4 0 43 43 59 696.4 0 59 44 66 696.4 0 66 45 56 696.4 0 56 46 52 696.4 0 52 47 149 696.4 0 149 48 103 696.4 0 103 49 52 696.4 0 52 50 52 696.4 0 52 51 42 696.4 0 42 52 54 696.4 0 54 53 66 696.4 0 66 54 227 696.4 0 227 55 96 696.4 0 96 56 32 696.4 0 32 57 73 696.4 0 73 58 90 696.4 0 90 59 36 696.4 0 36 60 51 696.4 0 51 61 50 696.4 0 50 62 64 696.4 0 64 63 32 696.4 0 32 64 36 696.4 0 36 65 44 696.4 0 44 66 39 696.4 0 39 67 62 696.4 0 62 68 108 696.4 0 108 69 64 696.4 0 64 70 83 696.4 0 83 71 38 696.4 0 38 72 57 696.4 0 57 73 80 696.4 0 80 74 60 696.4 0 60 75 125 696.4 0 125 76 62 696.4 0 62 77 64 696.4 0 64 78 100 696.4 0 100 79 56 696.4 0 56 80 42 696.4 0 42 81 77 696.4 0 77 82 78 696.4 0 78 83 40 696.4 0 40 84 54 696.4 0 54 85 55 696.4 0 55 86 51 696.4 0 51 87 55 696.4 0 55 88 66 696.4 0 66 89 48 696.4 0 48 90 59 696.4 0 59 91 143 696.4 0 143 92 83 696.4 0 83 93 75 696.4 0 75 94 76 696.4 0 76 95 80 696.4 0 80 96 81 696.4 0 81 97 236 696.4 0 236 98 138 696.4 0 138 99 146 696.4 0 146 100 47 696.4 0 47 101 55 696.4 0 55 102 31 696.4 0 31 103 51 696.4 0 51 104 39 696.4 0 39 105 55 696.4 0 55 106 76 696.4 0 76 107 95 696.4 0 95 108 63 696.4 0 63 109 64 696.4 0 64 110 54 696.4 0 54 111 54 696.4 0 54 112 78 696.4 0 78 113 86 696.4 0 86 114 80 696.4 0 80 115 107 696.4 0 107 116 113 696.4 0 113 117 125 696.4 0 125 118 120 696.4 0 120 119 174 696.4 0 174 120 191 696.4 0 191 121 125 696.4 0 125 122 76 696.4 0 76 123 99 696.4 0 99 124 140 696.4 0 140 125 1157 696.4 0 1157 126 809 696.4 0 809 127 878 696.4 0 878 128 118 696.4 0 118 129 108 696.4 0 108 130 115 696.4 0 115 131 85 696.4 0 85 132 84 696.4 0 84 133 115 696.4 0 115 134 166 696.4 0 166 135 283 696.4 0 283 136 237 696.4 0 237 137 118 696.4 0 118 138 121 696.4 0 121 139 125 696.4 0 125 140 95 696.4 0 95 141 72 696.4 0 72 142 117 696.4 0 117 143 139 696.4 0 139 144 138 696.4 0 138 145 880 696.4 0 880 146 644 696.4 0 644 147 845 696.4 0 845 148 96 696.4 0 96 149 111 696.4 0 111 150 109 696.4 0 109 151 128 696.4 0 128 152 124 696.4 0 124 153 115 696.4 0 115 154 177 696.4 0 177 155 188 696.4 0 188 156 178 696.4 0 178 157 202 696.4 0 202 158 210 696.4 0 210 159 163 696.4 0 163 160 115 696.4 0 115 161 155 696.4 0 155 162 144 696.4 0 144 163 139 696.4 0 139 164 177 696.4 0 177 165 323 696.4 0 323 166 312 696.4 0 312 167 252 696.4 0 252 168 139 696.4 0 139 169 126 696.4 0 126 170 124 696.4 0 124 171 206 696.4 0 206 172 272 696.4 0 272 173 1184 696.4 0 1184 174 3218 696.4 0 3218 175 1772 696.4 0 1772 176 946 696.4 0 946 177 117 696.4 0 117 178 144 696.4 0 144 179 179 696.4 0 179 180 202 696.4 0 202 181 577 696.4 0 577 182 611 696.4 0 611 183 540 696.4 0 540 184 213 696.4 0 213 185 320 696.4 0 320 186 1352 696.4 0 1352 187 1010 696.4 0 1010 188 1350 696.4 0 1350 189 393 696.4 0 393 190 186 696.4 0 186 191 172 696.4 0 172 192 197 696.4 0 197 193 218 696.4 0 218 194 191 696.4 0 191 195 187 696.4 0 187 196 133 696.4 0 133 197 88 696.4 0 88 198 101 696.4 0 101 199 174 696.4 0 174 200 163 696.4 0 163 201 197 696.4 0 197 202 196 696.4 0 196 203 201 696.4 0 201 204 192 696.4 0 192 205 156 696.4 0 156 206 598 696.4 0 598 207 657 696.4 0 657 208 858 696.4 0 858 209 375 696.4 0 375 210 164 696.4 0 164 211 176 696.4 0 176 212 253 696.4 0 253 213 297 696.4 0 297 214 367 696.4 0 367 215 332 696.4 0 332 216 198 696.4 0 198 217 208 696.4 0 208 218 247 696.4 0 247 219 337 696.4 0 337 220 420 696.4 0 420 221 363 696.4 0 363 222 290 696.4 0 290 223 231 696.4 0 231 224 408 696.4 0 408 225 4007 696.4 0 4007 226 3194 696.4 0 3194 227 4250 696.4 0 4250 228 310 696.4 0 310 229 539 696.4 0 539 230 3440 696.4 0 3440 231 3299 696.4 0 3299 232 4132 696.4 0 4132 233 1517 696.4 0 1517 234 450 696.4 0 450 235 315 696.4 0 315 236 178 696.4 0 178 237 181 696.4 0 181 238 172 696.4 0 172 239 172 696.4 0 172 240 218 696.4 0 218 241 238 696.4 0 238 242 196 696.4 0 196 243 217 696.4 0 217 244 172 696.4 0 172 245 282 696.4 0 282 246 1003 696.4 0 1003 247 514 696.4 0 514 248 701 696.4 0 701 249 289 696.4 0 289 250 789 696.4 0 789 251 607 696.4 0 607 252 1488 696.4 0 1488 253 2330 696.4 0 2330 254 1650 696.4 0 1650 255 756 696.4 0 756 256 146 696.4 0 146 257 131 696.4 0 131 258 118 696.4 0 118 259 158 696.4 0 158 260 178 696.4 0 178 261 211 696.4 0 211 262 197 696.4 0 197 263 228 696.4 0 228 264 260 696.4 0 260 265 378 696.4 0 378 266 238 696.4 0 238 267 259 696.4 0 259 268 293 696.4 0 293 269 337 696.4 0 337 270 255 696.4 0 255 271 573 696.4 0 573 272 317 696.4 0 317 273 556 696.4 0 556 274 270 696.4 0 270 275 578 696.4 0 578 276 498 696.4 0 498 277 420 696.4 0 420 278 652 696.4 0 652 279 1963 696.4 0 1963 280 6074 696.4 0 6074 281 6629 696.4 0 6629 282 4055 696.4 0 4055 283 1207 696.4 0 1207 284 433 696.4 0 433 285 697 696.4 0 697 286 415 696.4 0 415 287 394 696.4 0 394 288 361 696.4 0 361 289 416 696.4 0 416 290 460 696.4 0 460 291 417 696.4 0 417 292 323 696.4 0 323 293 292 696.4 0 292 294 438 696.4 0 438 295 736 696.4 0 736 296 1006 696.4 0 1006 297 1374 696.4 0 1374 298 1773 696.4 0 1773 299 2744 696.4 0 2744 300 5778 696.4 0 5778 301 29094 696.4 0 29094 Finished in 3.40 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 76,630 Reads with adapters: 21,708 (28.3%) Reads that were too short: 180 (0.2%) Reads written (passing filters): 21,528 (28.1%) Total basepairs processed: 17,493,206 bp Total written (filtered): 5,914,145 bp (33.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 21708 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 15 0.0 3 0 0 8 7 16 238 0.0 3 0 198 25 15 17 20468 0.0 3 19074 1143 211 40 18 65 0.0 3 1 45 9 10 19 95 0.0 3 5 12 47 31 20 469 0.0 3 400 20 5 44 21 4 0.0 3 0 4 23 1 0.0 3 0 0 0 1 24 2 0.0 3 0 0 1 1 26 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 1 1 31 2 0.0 3 0 0 0 2 32 3 0.0 3 0 0 0 3 35 2 0.0 3 0 0 0 2 36 7 0.0 3 0 1 0 6 37 4 0.0 3 1 0 0 3 38 5 0.0 3 0 0 0 5 39 5 0.0 3 0 0 0 5 44 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 46 51 0.0 3 0 0 1 50 47 5 0.0 3 0 0 0 5 49 12 0.0 3 0 0 0 12 50 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 53 3 0.0 3 0 0 0 3 55 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 0 4 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 66 45 0.0 3 0 0 0 45 68 1 0.0 3 0 0 0 1 69 5 0.0 3 0 0 0 5 72 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 3 0.0 3 0 0 0 3 87 1 0.0 3 0 0 0 1 88 2 0.0 3 0 0 0 2 94 5 0.0 3 0 0 0 5 95 3 0.0 3 0 0 0 3 97 23 0.0 3 0 0 0 23 98 2 0.0 3 0 0 0 2 99 2 0.0 3 0 0 0 2 100 10 0.0 3 0 0 0 10 103 2 0.0 3 0 0 0 2 105 4 0.0 3 0 0 0 4 106 3 0.0 3 0 0 1 2 110 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 114 2 0.0 3 0 0 0 2 115 4 0.0 3 0 0 0 4 116 8 0.0 3 0 0 1 7 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 119 4 0.0 3 0 0 0 4 122 1 0.0 3 0 0 0 1 123 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 131 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 1 1 147 8 0.0 3 0 0 0 8 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 155 3 0.0 3 0 0 0 3 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 160 3 0.0 3 0 0 0 3 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 0 5 165 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 177 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 1 1 182 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 1 1 188 1 0.0 3 1 195 2 0.0 3 0 0 0 2 208 1 0.0 3 0 1 211 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 8 0.0 3 0 0 0 8 283 4 0.0 3 0 0 0 4 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.76 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 21,528 Reads with adapters: 430 (2.0%) Reads written (passing filters): 21,528 (100.0%) Total basepairs processed: 5,914,145 bp Total written (filtered): 5,912,794 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 430 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.3% C: 15.3% G: 19.1% T: 3.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 377 336.4 0 377 4 46 84.1 0 46 5 6 21.0 0 6 6 1 5.3 0 1