This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 75.10 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 192,096 (6.7%) Reads that were too short: 121,282 (4.3%) Reads written (passing filters): 70,814 (2.5%) Total basepairs processed: 858,556,748 bp Total written (filtered): 17,120,865 bp (2.0%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 192096 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 845 696.4 0 845 7 687 696.4 0 687 8 18734 696.4 0 18734 9 21145 696.4 0 21145 10 109 696.4 0 109 11 108 696.4 0 108 12 48 696.4 0 48 13 84 696.4 0 84 14 85 696.4 0 85 15 53 696.4 0 53 16 47 696.4 0 47 17 72 696.4 0 72 18 722 696.4 0 722 19 48 696.4 0 48 20 69 696.4 0 69 21 103 696.4 0 103 22 32 696.4 0 32 23 29 696.4 0 29 24 64 696.4 0 64 25 39 696.4 0 39 26 49 696.4 0 49 27 48 696.4 0 48 28 57 696.4 0 57 29 29 696.4 0 29 30 72 696.4 0 72 31 74 696.4 0 74 32 30 696.4 0 30 33 46 696.4 0 46 34 39 696.4 0 39 35 47 696.4 0 47 36 41 696.4 0 41 37 49 696.4 0 49 38 84 696.4 0 84 39 40 696.4 0 40 40 51 696.4 0 51 41 38 696.4 0 38 42 41 696.4 0 41 43 63 696.4 0 63 44 51 696.4 0 51 45 69 696.4 0 69 46 1424 696.4 0 1424 47 190 696.4 0 190 48 119 696.4 0 119 49 47 696.4 0 47 50 51 696.4 0 51 51 42 696.4 0 42 52 68 696.4 0 68 53 36 696.4 0 36 54 214 696.4 0 214 55 94 696.4 0 94 56 44 696.4 0 44 57 67 696.4 0 67 58 78 696.4 0 78 59 31 696.4 0 31 60 35 696.4 0 35 61 52 696.4 0 52 62 49 696.4 0 49 63 31 696.4 0 31 64 54 696.4 0 54 65 40 696.4 0 40 66 52 696.4 0 52 67 37 696.4 0 37 68 63 696.4 0 63 69 51 696.4 0 51 70 55 696.4 0 55 71 59 696.4 0 59 72 65 696.4 0 65 73 34 696.4 0 34 74 61 696.4 0 61 75 69 696.4 0 69 76 50 696.4 0 50 77 57 696.4 0 57 78 81 696.4 0 81 79 59 696.4 0 59 80 35 696.4 0 35 81 45 696.4 0 45 82 69 696.4 0 69 83 55 696.4 0 55 84 64 696.4 0 64 85 67 696.4 0 67 86 78 696.4 0 78 87 80 696.4 0 80 88 72 696.4 0 72 89 64 696.4 0 64 90 58 696.4 0 58 91 66 696.4 0 66 92 64 696.4 0 64 93 72 696.4 0 72 94 85 696.4 0 85 95 85 696.4 0 85 96 70 696.4 0 70 97 136 696.4 0 136 98 71 696.4 0 71 99 135 696.4 0 135 100 59 696.4 0 59 101 42 696.4 0 42 102 45 696.4 0 45 103 61 696.4 0 61 104 53 696.4 0 53 105 57 696.4 0 57 106 92 696.4 0 92 107 100 696.4 0 100 108 68 696.4 0 68 109 47 696.4 0 47 110 50 696.4 0 50 111 62 696.4 0 62 112 76 696.4 0 76 113 69 696.4 0 69 114 77 696.4 0 77 115 80 696.4 0 80 116 102 696.4 0 102 117 112 696.4 0 112 118 107 696.4 0 107 119 170 696.4 0 170 120 178 696.4 0 178 121 110 696.4 0 110 122 85 696.4 0 85 123 117 696.4 0 117 124 120 696.4 0 120 125 998 696.4 0 998 126 748 696.4 0 748 127 827 696.4 0 827 128 100 696.4 0 100 129 83 696.4 0 83 130 81 696.4 0 81 131 76 696.4 0 76 132 58 696.4 0 58 133 76 696.4 0 76 134 105 696.4 0 105 135 178 696.4 0 178 136 181 696.4 0 181 137 87 696.4 0 87 138 106 696.4 0 106 139 93 696.4 0 93 140 66 696.4 0 66 141 79 696.4 0 79 142 83 696.4 0 83 143 93 696.4 0 93 144 81 696.4 0 81 145 492 696.4 0 492 146 458 696.4 0 458 147 520 696.4 0 520 148 58 696.4 0 58 149 86 696.4 0 86 150 84 696.4 0 84 151 96 696.4 0 96 152 112 696.4 0 112 153 79 696.4 0 79 154 106 696.4 0 106 155 110 696.4 0 110 156 117 696.4 0 117 157 125 696.4 0 125 158 120 696.4 0 120 159 106 696.4 0 106 160 95 696.4 0 95 161 99 696.4 0 99 162 116 696.4 0 116 163 103 696.4 0 103 164 138 696.4 0 138 165 195 696.4 0 195 166 158 696.4 0 158 167 154 696.4 0 154 168 109 696.4 0 109 169 113 696.4 0 113 170 106 696.4 0 106 171 183 696.4 0 183 172 209 696.4 0 209 173 1026 696.4 0 1026 174 2344 696.4 0 2344 175 1350 696.4 0 1350 176 528 696.4 0 528 177 75 696.4 0 75 178 113 696.4 0 113 179 154 696.4 0 154 180 156 696.4 0 156 181 379 696.4 0 379 182 490 696.4 0 490 183 376 696.4 0 376 184 177 696.4 0 177 185 230 696.4 0 230 186 608 696.4 0 608 187 607 696.4 0 607 188 667 696.4 0 667 189 175 696.4 0 175 190 100 696.4 0 100 191 146 696.4 0 146 192 150 696.4 0 150 193 163 696.4 0 163 194 139 696.4 0 139 195 104 696.4 0 104 196 114 696.4 0 114 197 64 696.4 0 64 198 67 696.4 0 67 199 105 696.4 0 105 200 124 696.4 0 124 201 127 696.4 0 127 202 127 696.4 0 127 203 142 696.4 0 142 204 139 696.4 0 139 205 113 696.4 0 113 206 234 696.4 0 234 207 249 696.4 0 249 208 426 696.4 0 426 209 160 696.4 0 160 210 109 696.4 0 109 211 137 696.4 0 137 212 219 696.4 0 219 213 186 696.4 0 186 214 273 696.4 0 273 215 257 696.4 0 257 216 159 696.4 0 159 217 132 696.4 0 132 218 179 696.4 0 179 219 218 696.4 0 218 220 275 696.4 0 275 221 238 696.4 0 238 222 193 696.4 0 193 223 198 696.4 0 198 224 328 696.4 0 328 225 4166 696.4 0 4166 226 3245 696.4 0 3245 227 5119 696.4 0 5119 228 222 696.4 0 222 229 458 696.4 0 458 230 1566 696.4 0 1566 231 1536 696.4 0 1536 232 1498 696.4 0 1498 233 701 696.4 0 701 234 210 696.4 0 210 235 168 696.4 0 168 236 148 696.4 0 148 237 131 696.4 0 131 238 114 696.4 0 114 239 125 696.4 0 125 240 104 696.4 0 104 241 128 696.4 0 128 242 136 696.4 0 136 243 141 696.4 0 141 244 140 696.4 0 140 245 172 696.4 0 172 246 647 696.4 0 647 247 336 696.4 0 336 248 566 696.4 0 566 249 157 696.4 0 157 250 473 696.4 0 473 251 405 696.4 0 405 252 973 696.4 0 973 253 1187 696.4 0 1187 254 832 696.4 0 832 255 377 696.4 0 377 256 113 696.4 0 113 257 106 696.4 0 106 258 119 696.4 0 119 259 136 696.4 0 136 260 142 696.4 0 142 261 121 696.4 0 121 262 117 696.4 0 117 263 155 696.4 0 155 264 229 696.4 0 229 265 637 696.4 0 637 266 230 696.4 0 230 267 230 696.4 0 230 268 285 696.4 0 285 269 243 696.4 0 243 270 202 696.4 0 202 271 378 696.4 0 378 272 318 696.4 0 318 273 447 696.4 0 447 274 350 696.4 0 350 275 918 696.4 0 918 276 597 696.4 0 597 277 215 696.4 0 215 278 262 696.4 0 262 279 553 696.4 0 553 280 1738 696.4 0 1738 281 2031 696.4 0 2031 282 1160 696.4 0 1160 283 584 696.4 0 584 284 647 696.4 0 647 285 1280 696.4 0 1280 286 690 696.4 0 690 287 606 696.4 0 606 288 550 696.4 0 550 289 552 696.4 0 552 290 712 696.4 0 712 291 562 696.4 0 562 292 424 696.4 0 424 293 385 696.4 0 385 294 608 696.4 0 608 295 978 696.4 0 978 296 1536 696.4 0 1536 297 2262 696.4 0 2262 298 3368 696.4 0 3368 299 5217 696.4 0 5217 300 10111 696.4 0 10111 301 47306 696.4 0 47306 Finished in 3.52 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 70,814 Reads with adapters: 22,376 (31.6%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 22,358 (31.6%) Total basepairs processed: 17,120,865 bp Total written (filtered): 6,012,095 bp (35.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 22376 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 9 0.0 3 0 0 0 1 8 18 10 0.0 3 0 0 10 19 241 0.0 3 0 237 3 0 1 20 21345 0.0 4 20952 381 11 1 21 64 0.0 4 10 50 4 22 6 0.0 4 2 0 3 0 1 23 19 0.0 4 0 0 0 17 2 24 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 39 1 0.0 4 1 41 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 1 69 2 0.0 4 0 0 0 0 2 96 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 1 110 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 635 0.0 4 0 0 0 0 635 128 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 130 7 0.0 4 0 0 0 0 7 143 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 1 188 2 0.0 4 2 196 1 0.0 4 0 0 1 203 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 22,358 Reads with adapters: 4,682 (20.9%) Reads written (passing filters): 22,358 (100.0%) Total basepairs processed: 6,012,095 bp Total written (filtered): 5,968,250 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4682 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.5% C: 2.2% G: 96.0% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 4 349.3 0 4 4 2 87.3 0 2 6 53 5.5 0 53 8 2 0.3 0 2 9 3216 0.1 0 97 3119 10 1278 0.0 1 36 1242 11 119 0.0 1 4 115 12 4 0.0 1 0 4 13 2 0.0 1 0 2 161 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.