This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 75.13 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,852,348 Reads with adapters: 116,489 (4.1%) Reads that were too short: 25,348 (0.9%) Reads written (passing filters): 91,141 (3.2%) Total basepairs processed: 858,556,748 bp Total written (filtered): 24,961,516 bp (2.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 116489 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1520 696.4 0 1520 7 38306 696.4 0 38306 8 33782 696.4 0 33782 9 64 696.4 0 64 10 40 696.4 0 40 11 39 696.4 0 39 12 36 696.4 0 36 13 56 696.4 0 56 14 47 696.4 0 47 15 39 696.4 0 39 16 39 696.4 0 39 17 33 696.4 0 33 18 42 696.4 0 42 19 43 696.4 0 43 20 39 696.4 0 39 21 51 696.4 0 51 22 45 696.4 0 45 23 49 696.4 0 49 24 38 696.4 0 38 25 46 696.4 0 46 26 74 696.4 0 74 27 43 696.4 0 43 28 52 696.4 0 52 29 38 696.4 0 38 30 27 696.4 0 27 31 43 696.4 0 43 32 43 696.4 0 43 33 41 696.4 0 41 34 45 696.4 0 45 35 38 696.4 0 38 36 30 696.4 0 30 37 41 696.4 0 41 38 79 696.4 0 79 39 437 696.4 0 437 40 482 696.4 0 482 41 1415 696.4 0 1415 42 382 696.4 0 382 43 41 696.4 0 41 44 29 696.4 0 29 45 33 696.4 0 33 46 47 696.4 0 47 47 30 696.4 0 30 48 59 696.4 0 59 49 50 696.4 0 50 50 44 696.4 0 44 51 45 696.4 0 45 52 73 696.4 0 73 53 57 696.4 0 57 54 34 696.4 0 34 55 41 696.4 0 41 56 31 696.4 0 31 57 49 696.4 0 49 58 46 696.4 0 46 59 32 696.4 0 32 60 51 696.4 0 51 61 58 696.4 0 58 62 34 696.4 0 34 63 25 696.4 0 25 64 45 696.4 0 45 65 71 696.4 0 71 66 46 696.4 0 46 67 60 696.4 0 60 68 41 696.4 0 41 69 41 696.4 0 41 70 65 696.4 0 65 71 71 696.4 0 71 72 76 696.4 0 76 73 37 696.4 0 37 74 35 696.4 0 35 75 41 696.4 0 41 76 57 696.4 0 57 77 38 696.4 0 38 78 37 696.4 0 37 79 40 696.4 0 40 80 37 696.4 0 37 81 44 696.4 0 44 82 45 696.4 0 45 83 37 696.4 0 37 84 109 696.4 0 109 85 82 696.4 0 82 86 78 696.4 0 78 87 43 696.4 0 43 88 50 696.4 0 50 89 63 696.4 0 63 90 68 696.4 0 68 91 66 696.4 0 66 92 126 696.4 0 126 93 100 696.4 0 100 94 54 696.4 0 54 95 69 696.4 0 69 96 68 696.4 0 68 97 62 696.4 0 62 98 341 696.4 0 341 99 428 696.4 0 428 100 403 696.4 0 403 101 90 696.4 0 90 102 60 696.4 0 60 103 50 696.4 0 50 104 55 696.4 0 55 105 78 696.4 0 78 106 53 696.4 0 53 107 44 696.4 0 44 108 50 696.4 0 50 109 55 696.4 0 55 110 97 696.4 0 97 111 193 696.4 0 193 112 132 696.4 0 132 113 181 696.4 0 181 114 43 696.4 0 43 115 48 696.4 0 48 116 43 696.4 0 43 117 68 696.4 0 68 118 61 696.4 0 61 119 56 696.4 0 56 120 62 696.4 0 62 121 51 696.4 0 51 122 37 696.4 0 37 123 78 696.4 0 78 124 90 696.4 0 90 125 101 696.4 0 101 126 59 696.4 0 59 127 61 696.4 0 61 128 56 696.4 0 56 129 58 696.4 0 58 130 71 696.4 0 71 131 56 696.4 0 56 132 91 696.4 0 91 133 87 696.4 0 87 134 100 696.4 0 100 135 206 696.4 0 206 136 72 696.4 0 72 137 85 696.4 0 85 138 90 696.4 0 90 139 81 696.4 0 81 140 51 696.4 0 51 141 62 696.4 0 62 142 63 696.4 0 63 143 122 696.4 0 122 144 82 696.4 0 82 145 73 696.4 0 73 146 129 696.4 0 129 147 75 696.4 0 75 148 58 696.4 0 58 149 64 696.4 0 64 150 61 696.4 0 61 151 62 696.4 0 62 152 68 696.4 0 68 153 83 696.4 0 83 154 60 696.4 0 60 155 60 696.4 0 60 156 77 696.4 0 77 157 85 696.4 0 85 158 305 696.4 0 305 159 315 696.4 0 315 160 166 696.4 0 166 161 55 696.4 0 55 162 83 696.4 0 83 163 85 696.4 0 85 164 73 696.4 0 73 165 54 696.4 0 54 166 54 696.4 0 54 167 72 696.4 0 72 168 74 696.4 0 74 169 105 696.4 0 105 170 127 696.4 0 127 171 183 696.4 0 183 172 255 696.4 0 255 173 67 696.4 0 67 174 52 696.4 0 52 175 51 696.4 0 51 176 80 696.4 0 80 177 163 696.4 0 163 178 238 696.4 0 238 179 189 696.4 0 189 180 120 696.4 0 120 181 77 696.4 0 77 182 65 696.4 0 65 183 146 696.4 0 146 184 159 696.4 0 159 185 208 696.4 0 208 186 96 696.4 0 96 187 92 696.4 0 92 188 134 696.4 0 134 189 173 696.4 0 173 190 96 696.4 0 96 191 85 696.4 0 85 192 70 696.4 0 70 193 56 696.4 0 56 194 72 696.4 0 72 195 92 696.4 0 92 196 84 696.4 0 84 197 62 696.4 0 62 198 75 696.4 0 75 199 64 696.4 0 64 200 64 696.4 0 64 201 74 696.4 0 74 202 79 696.4 0 79 203 93 696.4 0 93 204 89 696.4 0 89 205 63 696.4 0 63 206 76 696.4 0 76 207 85 696.4 0 85 208 71 696.4 0 71 209 75 696.4 0 75 210 167 696.4 0 167 211 213 696.4 0 213 212 140 696.4 0 140 213 150 696.4 0 150 214 604 696.4 0 604 215 736 696.4 0 736 216 1018 696.4 0 1018 217 147 696.4 0 147 218 57 696.4 0 57 219 62 696.4 0 62 220 68 696.4 0 68 221 78 696.4 0 78 222 92 696.4 0 92 223 557 696.4 0 557 224 992 696.4 0 992 225 467 696.4 0 467 226 112 696.4 0 112 227 147 696.4 0 147 228 89 696.4 0 89 229 72 696.4 0 72 230 57 696.4 0 57 231 62 696.4 0 62 232 63 696.4 0 63 233 76 696.4 0 76 234 79 696.4 0 79 235 85 696.4 0 85 236 72 696.4 0 72 237 82 696.4 0 82 238 134 696.4 0 134 239 155 696.4 0 155 240 118 696.4 0 118 241 114 696.4 0 114 242 122 696.4 0 122 243 109 696.4 0 109 244 125 696.4 0 125 245 95 696.4 0 95 246 86 696.4 0 86 247 75 696.4 0 75 248 64 696.4 0 64 249 75 696.4 0 75 250 70 696.4 0 70 251 85 696.4 0 85 252 79 696.4 0 79 253 97 696.4 0 97 254 75 696.4 0 75 255 84 696.4 0 84 256 208 696.4 0 208 257 254 696.4 0 254 258 297 696.4 0 297 259 216 696.4 0 216 260 105 696.4 0 105 261 106 696.4 0 106 262 119 696.4 0 119 263 648 696.4 0 648 264 340 696.4 0 340 265 327 696.4 0 327 266 86 696.4 0 86 267 67 696.4 0 67 268 103 696.4 0 103 269 100 696.4 0 100 270 181 696.4 0 181 271 413 696.4 0 413 272 3210 696.4 0 3210 273 2608 696.4 0 2608 274 1725 696.4 0 1725 275 148 696.4 0 148 276 275 696.4 0 275 277 285 696.4 0 285 278 379 696.4 0 379 279 116 696.4 0 116 280 87 696.4 0 87 281 66 696.4 0 66 282 140 696.4 0 140 283 262 696.4 0 262 284 417 696.4 0 417 285 427 696.4 0 427 286 297 696.4 0 297 287 134 696.4 0 134 288 122 696.4 0 122 289 126 696.4 0 126 290 69 696.4 0 69 291 71 696.4 0 71 292 67 696.4 0 67 293 79 696.4 0 79 294 74 696.4 0 74 295 78 696.4 0 78 296 63 696.4 0 63 297 123 696.4 0 123 298 266 696.4 0 266 299 417 696.4 0 417 300 373 696.4 0 373 301 337 696.4 0 337 Finished in 4.42 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 91,141 Reads with adapters: 38,945 (42.7%) Reads that were too short: 97 (0.1%) Reads written (passing filters): 38,848 (42.6%) Total basepairs processed: 24,961,516 bp Total written (filtered): 10,737,355 bp (43.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 38945 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 21 0.0 3 0 0 13 8 16 334 0.0 3 0 289 27 18 17 38060 0.0 3 35423 2167 373 97 18 124 0.0 3 19 93 7 5 19 87 0.0 3 0 0 42 45 20 45 0.0 3 3 1 0 41 21 2 0.0 3 0 0 0 2 23 2 0.0 3 1 0 1 24 3 0.0 3 0 0 0 3 27 1 0.0 3 1 28 1 0.0 3 0 0 0 1 30 3 0.0 3 0 0 0 3 32 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 1 1 59 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 62 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 73 13 0.0 3 0 0 0 13 74 2 0.0 3 0 0 0 2 76 5 0.0 3 0 0 0 5 77 2 0.0 3 0 0 0 2 79 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 1 0.0 3 0 0 0 1 83 13 0.0 3 0 0 1 12 84 5 0.0 3 0 0 0 5 85 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 117 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 130 3 0.0 3 0 0 0 3 132 47 0.0 3 0 0 0 47 133 6 0.0 3 0 0 0 6 134 2 0.0 3 0 0 0 2 135 15 0.0 3 0 0 1 14 136 2 0.0 3 0 0 0 2 137 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 5 0.0 3 0 0 0 5 163 1 0.0 3 0 0 0 1 167 4 0.0 3 0 0 2 2 168 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 217 3 0.0 3 0 0 0 3 226 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 3 0.0 3 0 0 0 3 235 2 0.0 3 0 0 0 2 243 1 0.0 3 0 0 0 1 244 4 0.0 3 0 0 0 4 245 2 0.0 3 0 0 0 2 246 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 1 248 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 252 3 0.0 3 0 0 0 3 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 257 2 0.0 3 0 0 1 1 260 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 1 1 263 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 4 0.0 3 0 0 0 4 281 5 0.0 3 0 0 0 5 282 7 0.0 3 0 0 1 6 283 2 0.0 3 0 0 0 2 287 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.34 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 38,848 Reads with adapters: 585 (1.5%) Reads written (passing filters): 38,848 (100.0%) Total basepairs processed: 10,737,355 bp Total written (filtered): 10,735,540 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 585 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 13.5% C: 71.8% G: 10.6% T: 4.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 556 607.0 0 556 4 13 151.8 0 13 5 9 37.9 0 9 6 6 9.5 0 6 14 1 0.0 1 0 1